Wyniki wyszukiwania - Biblioteka Nauki

Nowa wersja platformy, zawierająca wyłącznie zasoby pełnotekstowe, jest już dostępna.
Przejdź na https://bibliotekanauki.pl

Ograniczanie wyników

Znaleziono wyników: 3

Liczba wyników na stronie

Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych: mycolic acids

Sortuj według:

Ogranicz wyniki do:

Mycolic Acids as Markers of Osseous Tuberculosis in the Neolithic Skeleton from Kujawy Region (Central Poland)

100%

Borowska-Strugiń B. , Druszczyńska M. , Lorkiewicz W. , Szewczyk R. , Ządzińska E.

Anthropological Review

2014

tom 77

nr 2

137-149

The subject of analysis is the male skeleton from a double burial of the Globular Amphora Culture, derived from the Neolithic site at Brześć Kujawski in Kujawy region (central Poland). Within the spine of the individual advanced lesions are observed (destruction of the vertebral bodies, symptoms of the periostitis in the thoracic region) which are characteristic of skeletal tuberculosis. To check whether the observed morphological changes resulted from infection with Mycobacterium tuberculosis (M.tb), the bone material was tested positively for the presence of mycolic acids, the specific components of the cell wall of pathogenic M.tb bacilli, by mass spectrometry.

Computerized "mycobacterium" type recognition using numerical data base of mycolic acids elution profiles

63%

Glapiński J. , Walkiewicz R. , Safianowska A. , Grubek-Jaworska H.

Biocybernetics and Biomedical Engineering

2004

tom Vol. 24, no. 1

25-38

The aim of this study was to create a standarized numerical data base of high pressure liquid chromatography elution (HPLC) profiles of mycolic acids extracted from the "Mycobacterium" cell wall, with 28 mycobacterial American Type Culture Collection (ATCC) strains taken as a reference for the study. The final result of our work is a notebook of standarized elution profiles and a computer program (MYCAM) for highly effective and specific automatic "Mycobacterium" species identification.

HPLC analysis of mycolic acids in evaluation of drug susceptibility of Mycobacterium tuberculosis strains - comparison with conventional methods

63%

Walkiewicz R. , Chazan R. , Grubek-Jaworska H.

Biocybernetics and Biomedical Engineering

2010

tom Vol. 30, no. 2

3-14

The commonly used phenotypic tests of M. tuberculosis drug susceptibility evaluate drug effects on quantity of colony forming units (CFU) on solid media or CO2 secretion/O2 con-sumption by cultures in liquid media. These tests are not precise enough and need a long time for the growth of the bacilli, so new more accurate and rapid methods are highly needed. The aim of this study was to determine the utility of quantitative HPLC analysis of mycolic acids for drug susceptibility of M. tuberculosis. In 119/120 (99.16%) of the performed tests the HPLC methods showed excellent agreement with the conventional phenotypic tests. The quantitative HPLC analysis of mycolic acids is a fairly exact indicator of tubercle bacilli growth and may be used as a quick and reliable measure of their drug sensitivity.