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1

Zmiany zawartości ergosterolu podczas przechowywania pszenicy durum

100%

Stuper K. , Gawrysiak-Witulska M. , Szwed G. , Perkowski J.

Aparatura Badawcza i Dydaktyczna

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2011

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tom T. 16, nr 2

107-111

PL

Celem przedstawionych badań, było monitorowanie zmian zawartości grzybów mikroskopowych mierzonej stężeniem ERG w czasie przechowywania ziarna pszenicy twardej w warunkach różnej wilgotności ziarna, w warunkach niskiej temperatury, przy wysokiej wilgotności oraz wysokiej temperaturze i niskiej wilgotności. W pierwszym doświadczeniu materiał badany stanowiło ziarno krajowej pszenicy ozimej, hodowlanej linii Desf. Triticulum durum LGR 626b/99/4, wyprowadzonej w Instytucie Genetyki i Hodowli Roślin AR w Lublinie. Ziarno o wilgotności 9%, 14% oraz 18%, przechowywano w temperaturze 20 stopni Celsjusza przez okres 3 miesięcy. Pobraną próbę rozdrabniano i oznaczano stężenie ERG. Zawartość ERG w próbie kontrolnej wynosiła 1,72 mg/kg. W przypadku wszystkich badanych prób o różnej, przedstawionej powyżej wilgotności liczebność grzybów zmieniała się w czasie. Po 3 miesiącach przechowywania stężenie ERG (w mg/kg) wynosiło odpowiednio dla 9% 2,27,14% 5,88, dla 18% natomiast 11,15. W drugim doświadczeniu badano ziarno pochodzące z punktów skupu zbóż znajdujących się na terenie zachodnio- południowej Polski. Ziarno o wilgotności 14% przechowywano w różnych warunkach. Stanowisko A: temperatura 18 stopni Celsjusza oraz wilgotność względna powietrza 45% oraz stanowisko B: temperatura 10 stopni Celsjusza oraz wilgotność względna powietrza 80%. Na podstawie otrzymanych wyników stwierdzono, iż nawet niewielki wzrost wilgotności ziarna zbóż, przy zachowaniu stałej temperatury, wpływa w istotny sposób na poziom grzybów mikroskopowych w czasie przechowywania prób pszenicy durum. Stwierdzono również, iż wilgotność względna powietrza w miejscu przechowywania istotniej niż temperatura wpływa na rozwój pleśni.

EN

The aim of the presented investigations was to monitor changes in contents of mycoflora measured by ERG concentration during storage of durum wheat grain under conditions of different grain moisture content, maintaining constant temperature, and under low temperature conditions at high moisture content and low humidity. In the first experiment the experimental material consisted of spring wheat grain grown in Poland, of a breeding line Desf. Triticulum durum LGR 626b/99/4, produced at the Institute of Genetics and Plant Breeding, the Agricultural University in Lublin. Grain with moisture content of 9%, 14% and 18% was stored at 20 degrees of Celsius for 3 months. Collected samples were milled and ERG concentration was determined. The content of ERG in the control was 1.72 mg/kg. In case of all tested samples with different moisture contents, presented above, the level of mycoflora changed in time. After 3-month storage ERG concentration (in mg/kg) for 9% moisture content was 2.27, for 14% it was 5.88, while for 18% it was 11.15 mg/kg. 108 In the second experiment analyses were conducted on grain coming from grain purchasing centres in southwestern Poland. Grain with moisture content of 14% was stored under different conditions. Station A: temperature 18 degrees of Celsius and relative humidity 45%, and station B: temperature 10 degrees of Celsius and relative humidity 80%. Based on results it was found that even a slight increase in moisture content of grain when maintaining constant temperature has a significant effect on the level of mycoflora during storage of durum wheat samples. It was also found that relative humidity in the storage site has a more significant effect on the development of mycoflora than temperature does.

2

Impact of industrial waste water effluents on mycoflora of the shore sediments of the 3rd oxidation pond, with reference to biosorption of heavy metals

75%

Sharaf E. F.

Acta Microbiologica Polonica

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2002

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tom 51

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nr 3

3

Effect of crop rotation on stalk rot development and composition of mycoflora in maize stalk

75%

Palaversic B. , Pucaric A. , Jukic M. , Brekalo J.

Genetica Polonica

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1994

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tom 35B

4

The effect of long-term mineral fertilization on the mycoflora and chitinolytic activity of agricultural soils

75%

Popowska-Nowak E. , Koval E. Z. , Kharkevich E. S.

Polish Ecological Studies

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1995

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tom 21

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nr 1

5

Fungi inhabiting cancerations on apple shoots and their application against Nectria galligena Bres.

63%

Grabowski M. , Les B.

Phytopathologia Polonica

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1996

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tom 11

6

New aquatic sites of the fungus Sommerstorffia spinosa

63%

Czeczuga B. , Mazalska B. , Orlowska M.

Acta Mycologica

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2000

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tom 35

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nr 2

7

Volvariella surrecta - a new species in the mycoflora of Poland

63%

Celka D.

Acta Mycologica

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2000

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tom 35

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nr 2

8

Mycological flora on tree fruits, crust, leaves and pollen Sorbus domestica L.

63%

Kacaniova M. , Fikselova M.

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nr 2

9

Prevalence of microscopic filamentous fungi in poultry processing plant and concentration of aflatoxin B1 and ochratoxin A in eggs

63%

Laciakova A. , Mate D. , Pipova M. , Pastorova B. , Laciak V. , Bakosova A.

Bulletin of the Veterinary Institute in Puławy

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2001

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tom 45

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nr 1

EN

The composition of mycoflora in storage rooms, and other rooms in a poultry-processing plant, as well as on the surfaces of egg shells was observed. The concentrations of both aflatoxin B₁ and ochratoxin A were determined in the shell eggs at room temperature and humidity, at a higher temperature and humidity, and in the eggs previously contaminated by Aspergillus flavus. We found that there was a reciprocal correlation between the presence of microscopic filamentous fungi in the air and on the working tables (Cladosporium spp. 45.5%, Penicillium spp. 36.4%, Mucor spp. 9.0%). The penetration of mycotoxins through the egg shell was relatively low and the residue limit of aflatoxin B₁ allowed (5 μg.kg⁻¹) was not exceeded in any sample of egg tested. However, the residue limit of ochratoxin A (20 μg.kg⁻¹) was exceeded in one case.

10

The effect of pea [Pisum sativum L.] seed mycoflora on their germination capacity and electroconductivity

63%

Tylkowska K. , Ratajczak K.

Folia Horticulturae

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1993

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tom 05

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nr 1

11

Mycological analysis of cereal samples and screening of Fusarium strains' ability to form deoxynivalenole [DON] and zearalenone [ZEA] mycotoxins - a pilot study

63%

Klyszejko A. , Kubus Z. , Zakowska Z.

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nr Suppl.

EN

Filamentous fungi are cosmopolitan microorganisms found in almost all environments. It should be pointed out that occurance of moulds on food or feed may cause health disorders in humans and animals. Mycoflora appears as a source of toxic methabolites, mycotoxins, which hepatotoxic, genotoxic, nefrotoxic and carcinogenic abilities were already proven in several studies. Hense mycological analysis of cereal grains raises as an important manner in evaluation of food and feed health features. Among the most frequent cereal contaminants Alternaria, Aspergillus, Fusarium and Penicillium strains are mentioned. Due to their ability to grow on cereals during both its field growth and storage, Fusarium moulds occure to be an important contamination factors in food and feed industry. In this study Fusarium strains isolates from wheat and maize were examined in order to recognize their abilities to produce two toxins: zearalenon (ZEA) and deoxynivalenole (DON). Mycological analysis shown differentiation within fungal microflora occuring in samples of different storage conditions, where Fusarium strains represented aproximately 20-70% of all mould species present. In purpose of Fusarium strains species evaluation, isolates were mycologically analysed. In the second step of the project, toxicological screening of isolates was performed using Thin Liquid Chromatography (TLC) evaluating toxigenic potential of single strains' production of ZEA and DON. This data gives the possibility of pointing the most toxigenic strains and also shows differentiations in their occurance in cereals. This paper presents introductory research data, which can be useful in recognition of cereal contamination with moulds and their toxic methabolites.

12

Dynamics of human respiratory system mycoflora

63%

Biedunkiewicz A.

Acta Mycologica

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2001

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tom 36

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nr 2

13

Zoosporic fungi growing on the eggs of Coregonus lavaretus maraena [Bloch, 1779] from Lake Miedwie in Pomerania

63%

Czeczuga B. , Pietrucha M. , Muszynska E.

Acta Ichthyologica et Piscatoria

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2001

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tom 31

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nr 1

EN

The authors investigated of the mycoflora developing on the eggs of Miedwie whitefish (Coregonus lavaretus maraena). 32 aquatic zoosporic fungus species developed on the eggs, including 24 of the order Saprolegniales and 8 of the Peronosporales.

PL

Badano w warunkach laboratoryjnych występowanie pływkowych grzybów wodnych na ikrze siei miedwieńskiej (Coregonus lavaretus maraena) z jeziora Miedwie. Do doświadczeń używano wody z 6 różnych limnologicznie zbiorników, uwzględniając w niej poszczególne parametry hydrochemiczne. Ogólnie stwierdzono na ikrze siei miedwieńskiej rozwój 32 gatunków pływkowych grzybów wodnych, w tym dwa gatunki nowe dla hydromikologii Polski (Pythium acanthicum i Pythium catacearum).Wśród stwierdzonych gatunków 24 należały do rzędu Saprolegniales, zaś 8 do Peronosporales. Najmniej gatunków rozwijało się w najbardziej eutroficznej wodzie stawu Akcent (5), najwięcej -w wodzie jeziora Komosa (14).

14

Contamination of feed mixtures with mycoflora in South-Western Poland

51%

Kubizna J. , Jamroz D. , Kubizna J. K.

Electronic Journal of Polish Agricultural Universities. Series Veterinary Medicine

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2011

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tom 14

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nr 2

15

Occurrence of fungi in water distribution system

51%

Grabinska-Loniewska A. , Konillowicz-Kowalska T. , Wardzynska G. , Boryn K.

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nr 4

539-547

EN

It is demonstrated that contamination of water distribution systems with fungi is determined by the number and species composition of the mycoflora of waters supplying a given Water Treatment Plant (WTP) and the effectiveness of their removal in the unit processes of water treatment used. In the water distribution system examined, a significant number of microorganisms occurring in waters supplying the WTP was reduced in the water supplied to the system to 200 cfu l-1 following sorption, coagulation, filtration and disinfection processes. Their number did not exceed 267 cfu l-1 in the water phase in the distribution system while it was as many as 1000-5000-times greater in the biomass "suspended" in it. These organisms occurred sporadically in pipe sediments. Moulds, including species pathogenic and potentially pathogenic to humans and warm-blooded animals, constituted the mycoflora.

16

Pallasea quadrispinosa G.O.Sars specimens as vectors of aquatic zoosporic fungi parasiting on fish

51%

Czeczuga B. , Kiziewicz B. , Gruszka P.

Polish Journal of Environmental Studies

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2004

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tom 13

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nr 4

EN

The authors investigated the mycoflora developing on the dead specimens of Pallasea quadrispinosa from Lake Hańcza. Water for experiments was collected from four different bodies of water: Hańcza Lake, Wigry Lake, Supraśl River and Fosa Pond. A total of 41 zoosporic fungus species were found to grow on the dead of Pallasea quadrispinosa specimens, including 17 species inducing mycosis in a number of freshwater specimens, including 17 species inducing mycosis in a number of freshwater fish species: Achlya debaryana, A. diffusa, A. dubia, A. klebsiana, A. orion, A. polyandra, A. prolifera, A. proliferoides, Aphanomyces laevis, Dictyuchus monosporus, Leptolegnia caudata, Saprolegnia delica, S. ferax, S. hypogyna, S monoica, S. parasitica and and Thraustotheca clavata. Aphanomyces astaci as a parasite of noble as a parasite of noble crayfish, causing the so called crayfish plague, was also observed on Pallasea quadrispinosa.

17

Effect of gamma irradiation on elimination of aflatoxins produced by apple mycoflora in apple fruits

51%

Afifi A. F. , Foaad M. A. , Fawzi E. M.

Acta Microbiologica Polonica

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2003

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tom 52

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nr 4

18

Macromycetes of the proposed nature reserve Wilcze Uroczysko-Olszanka in the Odra estuary

51%

Friedrich S.

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nr 2

19

Micromycetes on Pisum sativum var. arvense

51%

Marcinkowska J.

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nr 1

20

Aquatic fungi growing on feathers of wild and domestic bird species in limnologically different water bodies

44%

Czeczuga B. , Godlewska A. , Kiziewicz B.

Polish Journal of Environmental Studies

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2004

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tom 13

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nr 1

EN

The mycoflora developing on the feathers of wild and domestic bird species in the water of 6 limnologically different water bodies was investigated under laboratory conditions. 97 zoosporic fungus species were found to grow on the feathers investigated, including 21 Chytridiomycetes, 1 Hyphochytriomycetes, 74 Oomycetes and 1 Zygomycetes fungus. The most common fungus species included Chytriomycetes annulatus, Rhizophydium keratinophilum, Blastocladiopsis parva, Catenaria anguillulae, Catenophlyctis variabilis, Aphanomyces helicoides, Aphanomyces irregularis, Leptolegniella piligena, Pythium afertile, Pythium aquatile, Pythium echinulatum, Pythium intermedium and Pythium tenue. The most fungi were noted growing in water from Cypisek spring (64), the fewest in the ponds Akcent (45) and Fosa (47 species). Out of these 97 species, 17 are known as parasites or necrotrophs of fish. 13 fungus species were recorded for the first time in Poland.