Wyniki wyszukiwania - Biblioteka Nauki

1

Pharmacokinetics and bioavailability of hapepunine in mice by ultra-performance liquid chromatography–tandem mass spectrometry

100%

Chen L. , Zhang Q. , Lin Y. , Lu X. , Zhong Z. , Ma J. , Wen C. , Ding C.

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tom Vol. 32, no. 1

44--48

EN

An ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) method was established to determine the hapepunine in mouse blood, and the pharmacokinetics of hapepunine after intravenous (1.0 mg/kg) and intragastric (2.5, 5, and 10 mg/kg) administrations was studied. Delavinone was used as an internal standard. The UPLC ethylene bridged hybrid (BEH) C18 column was used for chromatographic separation. The mobile phase consisted of acetonitrile and 0.1% formic acid with a gradient elution flow rate of 0.4 mL/min. Multiple reaction monitoring (MRM) mode was used for quantitative analysis of hapepunine in electrospray ionization (ESI) positive interface. Proteins from mouse blood were removed by acetonitrile precipitation. The verification method was established in accordance with the US Food and Drug Administration (FDA) bioanalytical method validation guidelines. In the concentration range of 1–1000 ng/mL, the hapepunine in the mouse blood was linear (r2 > 0.995), and the lower limit of quantification was 1.0 ng/mL. In the mouse blood, the intra-day precision coefficient of variation (CV) was less than 12%, the inter-day precision CV was less than 14%. The accuracy ranged from 91.7% to 109.3%. The average recovery was higher than 76.7%, and the matrix effect was between 86.0% and 106.4%. The UPLC–MS/MS method was sensitive, rapid, and selective and was successfully applied to the pharmacokinetic study of hapepunine in mice. The absolute bioavailability of hapepunine was 22.0%.

2

Pharmacokinetics of ebeiedinone in mouse blood by UPLC–MS/MS

100%

Jin Y. , Chen Y. , Liu J. , Bao X. , Zhi Y. , Wen C. , Zhu W.

Acta Chromatographica

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2020

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tom Vol. 32, no. 3

194--198

EN

An ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) method was established to determine ebeiedinone in mouse blood, and the pharmacokinetics of ebeiedinone after intravenous (0.5 mg/kg) and oral (2, 4, and 8 mg/kg) administration was studied. Twenty-four mice were randomly divided into 4 groups, 1 group was for intravenous administration (0.5 mg/kg), and other 3 groups were for oral administration (2, 4, and 8 mg/kg), with 6 rats in each group. Yubeinine was used as an internal standard. Multiple reaction monitoring (MRM) mode was used to quantitatively analyzed ebeiedinone m/z 414.4 → 91.1 and the internal standard m/z 430.4 → 412.3 in the electrospray ionization (ESI) positive interface. In the concentration range of 1–2000 ng/mL, the ebeiedinone in the mouse blood was linear (r2 > 0.995), and the lower limit of quantification was 1.0 ng/mL. In the mouse blood, the intra-day precision coefficient of variation (CV) was less than 15%, and the inter-day precision CV was less than 15%. The accuracy ranged from 85.4% to 114.6%, and the average recovery was higher than 61.3%. The matrix effect was between 87.0% and 106.5%. These data met the pharmacokinetic study requirements of ebeiedinone. The UPLC–MS/MS method was sensitive, rapid, and selective and was successfully applied to the pharmacokinetic study of ebeiedinone in mice. The absolute bioavailability of ebeiedinone was 30.6%.

3

Mouse Leydig cell culture on microcarriers

100%

Kmicikiewicz I. , Koziel E. , Pelczar U. , Pierscinski A. , Bilinska B.

Folia Histochemica et Cytobiologica. Supplement

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1997

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tom 35

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nr 2

4

The graft-versus-host [GvH] reaction and experimental trichinellosis. II. The effect of Trichinella spiralis on the local GvH reaction in the mouse popliteal lymph node

100%

Bany J.

Acta Parasitologica

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1992

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tom 37

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nr 2

EN

Activity of spleen lymphocytes derived from T. spiralis-infected C3H/w mice in the local GvH reaction was assessed using the popliteal lymph node GvH assay. It was found that splenocytes obtained on day 10 of the infection were substantially more active in the reaction than the spleen cells collected from uninfected donors. This effect correlated inversely with the infectious dose of the parasite, i.e. cells obtained from mice infected with 500 larvae per mouse were less efficient stimulators of the GvH reaction than splenocytes isolated from mice infected with 200 larvae per mouse. On day 30 of the infection activity of spleen T cells in the GvH reaction was suppressed in comparison to the control splenocytes but on day 60 post infection this activity returned to the baseline level. The above variations in the activity of splenic T lymphocytes in the local GvH reaction were readily quantitated by comparison of the three appropriate parameters assessed in the popliteal lymph nodes of both the infected and control animals and expressed as the mass, cellularity, and proliferation coefficients, respectively.

5

Pharmacokinetics and bioavailability of curdione in mice by UPLC-MS/MS

88%

Liang Q. , Chen T. , Luo L. , Ma Y. , Wen C. , Huang X.

Acta Chromatographica

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2023

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tom Vol. 35, no. 2

144--148

EN

A UPLC-MS/MS method was developed to determinate curdione in the mouse blood, and the pharmacokinetics of curdione in mice after intravenous (5 mg kg⁻¹) and oral (20 mg kg⁻¹) administration were studied. The HSS T3 column was used for separation, and column temperature was set at 40 °C. Multiple reaction monitoring (MRM) mode were used for determination of curdione. Blood samples were taken from the caudal vein of Institute of Cancer Research (ICR) mice after administration of curdione. It showed a good linear relationship in the range of 1–500 ng mL⁻¹ (r > 0.998); the intra-day precision was <13%, the inter-day precision was <15%, and the accuracy was 90%–105%, the recovery was >77%, and the matrix effect was 97%–107%. The half-life was relatively short, and the bioavailability was 6.5%. The developed method was suitable for the pharmacokinetics of curdione in mice.

6

Pharmacokinetics of 8-O-acetylharpagide in mouse blood by UPLC–MS/MS

88%

Chen S. , Huang M. , Yu Z. , He J. , Huang B. , Wang X. , Ma J. , Wen C.

Acta Chromatographica

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2019

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tom Vol. 31, no. 3

183--188

EN

8-O-Acetylharpagide is the main active component of the herb Ajuga decumbens, which possesses anti-tumor, anti-virus, and anti-inflammation properties. In this study, ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) was used to measure the concentration of 8-O-acetylharpagide in mouse blood, with subsequent investigation of the pharmacokinetics of the drug after intravenous or oral administration. Shanzhiside methyl ester was used as an internal standard, and the acetonitrile precipitation method was used to process the blood samples. Chromatographic separation was achieved using an ultra-performance liquid chromatography ethylene-bridged hybrid (UPLC BEH) column (2.1 mm × 50 mm, 1.7 μm) with a gradient methanol–water mobile phase (containing 0.1% formic acid). The flow rate was 0.4 mL/min, and the elution time was 5.0 min. 8-O-Acetylharpagide was quantitatively measured using electrospray ionization (ESI) tandem mass spectrometry in multiple reaction monitoring (MRM) mode with positive ionization. The result indicated that, within the range of 5–500 ng/mL, the linearity of 8-O-acetylharpagide in mouse blood was satisfactory (r > 0.995), and the lower limit of quantification (LLOQ) was 5 ng/mL. Intra-day precision relative standard deviation (RSD) of 8-O-acetylharpagide in blood was lower than 9%, and the inter-day precision RSD was lower than 13%. The accuracy range was between 94.3% and 107.1%, average recovery was higher than 91.3%, and the matrix effect was between 100.8% and 110.8%. This analytical method was sensitive and fast with good selectivity and was successfully applied to perform pharmacokinetic studies of 8-O-acetylharpagide in mice. The bioavailability of 8-O-acetylharpagide was 10.8%, and the analysis of the primary pharmacokinetic parameters after oral and intravenous administration indicated that 8-O-acetylharpagide had a significant first pass effect after oral administration.

7

Determination of eugenitin in mouse blood by ultra-performance liquid chromatography-tandem mass spectrometry and its application to a pharmacokinetic study

88%

Lin C. , Song D. , Jiang H. , Luo L. , Bao X. , Yu X. , Ma J. , Wang X. , Jiang X.

Acta Chromatographica

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2022

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tom Vol. 34, no. 3

253--257

EN

Eugenitin is a non-volatile chromone derivative which is always found in dried flower buds of Syzygium aromaticum L. (Merr.) & L.M. Perry. Until now, there were no reports about the pharmacokinetics of eugenitin in biological fluids. A UPLC-MS/MS method developed to determine eugenitin in mouse blood. The blood samples were prepared by protein precipitation with acetonitrile. Chrysin (internal standard, IS) and eugenitin were gradient eluted by mobile phase of acetonitrile and water (0.1% formic acid) in a BEH C18 column. The multiple reaction monitoring (MRM) of m/z 221.1→206.0 for eugenitin and m/z 255.1→152.9 for IS with an electrospray ionization (ESI) source was used for quantitative detection. The calibration curve ranged from 0.5 to 500 ng/mL (r > 0.995). The accuracy ranged from 98 to 113%, the precision was less than 12%, and the matrix effect was between 86 and 94%, the recovery was better than 81%. The developed method was successfully used for pharmacokinetics of eugenitin in mice after intravenous (5 mg/kg) and oral (20 mg/kg) administration, and the absolute availability of eugenitin was 12%.

8

Research on the possibility of treating streptococcal infections with hyperbaric oxygenation

88%

Wolański W.

Polish Hyperbaric Research

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2020

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tom nr 1(70)

43--46

EN

The aim of the study was to determine the effect of the application of hyperbaric oxygen therapy on the course of an infection with group A type T-3 hemolytic β streptococcus. Experiments were carried out on Porton white mice and in vitro blood plates. General and local infections with streptococci were induced in animals. The infected animals were treated with hyperbaric oxygenation. The lethal effect of infection was significantly inhibited using hyperbaric oxygenation on the first and second day following the infection.

9

IgA responses of various mouse strains to enteral infection with Trichinella spiralis

88%

Sinski E. , Szklarczyk J. , Bernat A. , Sztakowska I.

Acta Parasitologica

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1993

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tom 38

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nr 4

EN

The present study has examined the level of total IgA and antigen-specific IgA antibodies in serum and bile in various inbred strains of mice infected with Trichinella spiralis. These strains of mice differ in the speed at which they expel the adult worms from the gut. BALB/c and CBA mice expel adult worms faster than C3H and C57BL/6 mice. However, the CBA strain of mice is more resistant to the establishment of an initial infection of T. spiralis than BALB/c mice. While total serum IgA and bile sIgA concentrations correlated with the time course of the expulsion of adult worms, there was no similar correlation between IgA concentrations and the intensity of T. spiralis infection during the muscle phases of infection in any of the strains of mice investigated. Specific IgA antibodies in sera and sIgA antibodies in bile were measured by ELISA in C57BL/6 mice using crude somatic L1 muscle larvae (AgL1) and crude adult worm (AgAd) antigens. A pronounced increase in sIgA antibodies to AgL1 antigen was found by day 9 of infection in bile. However, a gradual increase in IgA in serum to AgAd antigen was observed from 6 till 24 DAI. Specific IgA response in serum to AgAd was much higher than to AgL1 and, in contrast the sIgA response in bile, was more pronounced to AgL1 than to AgAd. This result suggests that bile may also provide a valuable source of sIgA.

10

NMDA-glutamate mechanism of magnesium-induced anxiolytic-like activity

88%

Poleszak E. , Wlaz P. , Wrobel A. , Fidecka S. , Nowak G.

Journal of Elementology

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2008

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tom 13

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nr 3 Suppl.

11

Differences between C57BL-6 and C57BL-10 inbred mouse strains in chromosomes 2,4,13,17 and 18

88%

Gajewska M. , Rutkowski R. , Lukasiewicz D. , Wirth-Dzieciolowska E.

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tom 20

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nr 3

12

Ultrastructure of neurons of the otic ganglion in mouse

88%

Kuder T. , Slovakova D. , Mraz P.

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tom 40

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nr 1-4

13

Mice infected with Toxocara canis are resistant to challenge with the non-related helminth, Trichinella spiralis

88%

Ferens W. A. , Kayes S. G.

Acta Parasitologica

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1994

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tom 39

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nr 1

EN

Cross-resistance between Toxocara canis and Trichinella spiralis was studied in CBA/J mice exposed to varying doses of T. canis and 14 days later challenged with 400 larvae of T. spiralis. Intestinal burden of T. spiralis on day 7 post infection (PI) in mice given 25 ova of T. canis was 70% of challenge control burden, but in mice given 250 ova the burden was consistently below 20% of the control value. Male worms were preferentially expelled from mice exposed to T. canis. Recovery of muscle larvae was reduced in mice given 250 ova, but not in mice given 25 ova. Intestinal burdens of T. spiralis in T. canis-sensitized mice (250 ova) was 58% of the control values at 36 h PI, and most of the remaining worms were expelled between 5 and 7 days PI. Worms from mice given 250 ova released lower numbers of newborn larvae in vitro.

14

Morphometric analysis of the small intestine in wild type mice C57BL-6J - a developmental study

88%

Gulbinowicz M. , Berdel B. , Wojcik S. , Dziewiatkowski J. , Oikarinen S. , Mutanen M. , Kosma V. M. , Mykkanen H. , Morys J.

Folia Morphologica

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2004

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tom 63

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nr 4

EN

Recently the increasing prevalence of gastrointestinal diseases, including neoplasm, has resulted in the necessity of characterising not only the tumours, but also healthy mucosa. Research into the morphological changes of healthy mucosa under different experimental conditions, including drugs, special diets and the use of probiotic bacteria, is greatly facilitated by the availability of animal models. In spite of the widespread use of mice in gastrointestinal research, there is a lack of information on the qualitative and quantitative histological characteristics of the intestinal mucosa of the mouse. The aim of this study was to assess the morphological characteristics and the postnatal development of the small intestine of wild type mice — C57BL/6J. The mice were aged either 5 weeks or 12 weeks. The 12-week-old mice had been weaned at the age of 5 weeks. After dissection the small intestine was divided into 5 equal portions and randomly chosen microscopical sections from each were stained with haematoxylin and eosin. The parameters describing the morphology of the small intestine (villus height, depth of the crypt, villus width near the crypt, width of the villus connective tissue near the crypt, thickness of the muscular layer and the height of the enterocytes and their nuclei) were evaluated under a light microscope. In both age groups the height and width of the villi decreased, while the thickness of the muscular layer increased in the distal direction. The height of the enterocytes decreased and the height of the enterocyte nucleus increased towards the colon in both age groups. The depth of the crypts was greater in the younger animals than in the older ones. Our data provides the baseline morphological description of the small intestinal mucosa in wild type mice, strain C57BL/6J, which can be used as a reference for testing the influence of drugs, toxins, nutrients and inborn mutations on the mouse intestine.

15

Effect of Ascaris trypsin inhibitor on fetal development of mice

88%

Blaszkowska J.

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2005

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tom 51

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nr 2

EN

In this study, maternal toxicity and developmental effects of exposure to Ascaris trypsin inhibitor were evaluated in mice. Pregnant BALB/c females were injected intraperitoneally by Ascaris inhibitor /AIT/ at 200, 300, and 400 mg/kg body weight/day, on days 12 to 15 of gestation (stage of fetal development). At day 19 of pregnancy, uterine contents were inspected for implantation sites, early resorptions (moles), living fetuses and dead fetuses. The living fetuses were weighed and examined for external, internal and skeletal abnormalities. The results showed that AIT induced maternal toxicity, evidenced by maternal deaths, abortions, bleeding from uterus and reduced body weight gain as compared to control (p <0.01). There were no differences between the control group and the rest of all groups investigated for total implantation sites and early resorptions. Fetotoxicity was observed as shown by the decrease in the number of living fetuses and mean fetal weight, a high rate of intrauterine fetal deaths, delayed skeletal ossification, occurrence of pathological changes of fetal organs and tissues. Only one type of congenital malformations (hydronephrosis) was noted in fetuses after injection of higher doses of AIT.

16

Morphological heterogeneity of peroxisomes in cultured mouse Leydig cells

88%

Litwin J. A. , Bilinska B.

Folia Histochemica et Cytobiologica

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1995

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tom 33

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nr 4

17

An increased level of sperm abnormalities in mice with abnormal copper metabolism

88%

Kowal M.

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tom 42

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nr 1

18

Morphometric characteristic of the parameters of intestinal crypts during the postnatal development of Mus musculus L.

88%

Wilczynska B. , Przystalski A.

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nr 1

19

Immunomodulatory effects of Panax ginseng preparations on the mouse

88%

Rogala E. , Sommer E. , Radomska-Lesniewska D. , Sawicka T. , Prosinska J. , Drozd J. , Bialas-Chromiec B. , Filewska M. , Skopinska-Rozewska E.

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tom 50

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nr 1

EN

The immunomodulatory effect of two preparations from Panax ginseng radix, namely Ginsengcha (granulated mass of radix ginseng) and Ginsengpian (ginseng radix flakes) on mice has been investigated. The examined immune parameters were the following: the production of anti-SRBC antibodies, the chemokinesis of mouse spleen cells and the graft-versus-host reaction induced by mouse spleen cells. Additionally, the influence of ginseng preparations on the angiogenic activity of syngeneic LI sarcoma cells, LI sarcoma tumour growth and tumour blood supply (haemoglobin content) was examined. The production of antibodies was enhanced, particularly in the group with lower control parameters (pcO.OOl). Chemokinesis proved to be augmented (p<0.001) after 10 days of administering ginseng preparations to mice. The ability of mouse spleen cells to produce immunological mediators in the GvH reaction (immunological angiogenesis) turned out to increase significantly (p<0.001) under the influence of Ginsengcha. Neither Ginsengpian nor Ginsengcha stimulated the angiogenic activity of the LI sarcoma cells. Ginsengpian sligtly decreased this activity (p<0.02). The LI sarcoma tumour volume was significantly reduced by Ginsengcha (p<0.05), but no influence on tumour blood supply was observed. Panax ginseng preparations possess immunomodulatory properties and show potent antitumour activity.

PL

Badano wpływ dwóch preparatów z korzenia Panax ginseng: Ginsengcha (granulat) i Ginsengpian (płatki) na układ immunologiczny myszy. Oceniano produkcję przeciwciał anty-SRBC, aktywność chemo- kinetyczną komórek śledzion myszy, reakcję przeszczep przeciwko gospodarzowi (GvH) indukowaną komórkami śledzion mysich. Oceniano także wpływ preparatów żeń-szenia na aktywność angiogenną syngenicznych komórek LI sarcoma, wzrost i ukrwienie (zawartość hemoglobiny) guzów LI sarcoma. Uzyskane wyniki wykazały podwyższenie produkcji przeciwciał, zwłaszcza w grupie z niższymi wartościami kontrolnymi (p<0,001). Stwierdzono istotne (p<0,001) zwiększenie aktywności chemo- kinetycznej komórek mysich śledzion po 10 dniach podawania myszom preparatów żeń-szenia. Zdolność splenocytów do produkcji immunologicznych mediatorów oceniana w reakcji GvH (angiogene- za immunologiczna) ulegała istotnemu (p<0,001) podwyższeniu w wypadku zwierząt karmionych Ginsengcha. Oba badane preparaty nie pobudzały aktywności komórek LI sarcoma, a w wypadku Ginsengpian obserwowano obniżenie tej aktywności (p<0,02). Ginsengcha znacząco (p<0,05) zmniejszał objętość guzów LI sarcoma, ale nie wpływał na ukrwienie guzów. Preparaty Panax ginseng są im- munomodulatorami i wykazują aktywność przeciwnowotworową.

20

Echinococcus multilocularis: the effect of different numbers of pre-inoculation washings on the rate of development and amyloidogenesis of the metacestode in laboratory mice

88%

Alkarmi T. , Dar F. K. , Ali-Khan Z.

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nr 2

EN

The weight of the larval cyst mass (LCM), splenomegaly and the amount of splenic amyloid deposition, was shown to be dependant on a putative alveolar hydatid cyst (AHC) soluble component. The increase in LCM, spleen weight, and splenic amyloid was inversely proportional to the number of cyst washings during the preparation of the inoculum. A minimum of 8 washings were found necessary to induce significant reduction in parasite growth and amyloid deposition. With twenty four washings progressive phase of infection was aborted; while the restrictive phase was extended to 8 weeks. This finding was further confirmed by surgical transplantation of cysts obtained from infected mice at 12 weeks post infection to normal mice. The amount of splenic amyloid deposits, LCM and spleen weight after one week post-transplant was statistically not different from those in mice infected with AHC following 24 washings at 12 weeks post infection. The restrictive phase was eliminated following transplantation. AHC-extract (AHC-EXT) prepared from AHC before or after 24 washings was shown to retain its antigenicity and react with infected mice sera in the gel diffusion test. The reaction intensity and the number of bands seen were less after washing.