Wyniki wyszukiwania - Biblioteka Nauki

Nowa wersja platformy, zawierająca wyłącznie zasoby pełnotekstowe, jest już dostępna.
Przejdź na https://bibliotekanauki.pl

Ograniczanie wyników

1 Biocybernetics and Biomedical Engineering

1 2006

Znaleziono wyników: 1

Liczba wyników na stronie

Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych: monolayer

Sortuj według:

Ogranicz wyniki do:

3D matrigel culture improves differentiated functions of HepG2 cells in vitro

100%

Kinasiewicz J. , Kawiak J. , Weryński A.

tom Vol. 26, no. 4

47-54

In our study, the influence of culture conditions (monolayer and 3D Matrigel) of hepatocyte cell line (buman hepatoma cell line, HepG2) on their metabolic activity was assessed. Albumin synthesis by HepG2 cells was significantly higher in Matrigel culture as compared to monolayer culture of the cells. 3D culture improved urea production by HepG2 cells. The detoxification experiments revealed that HepG2 cells do not metabolize lidocaine neither in monolayer nor in 3D culture. 3D culture significantly improved cell polarity analyzed by F-actin staining and carboxyfluorescein excretion. In conclusion, the 3D Matrigel culture improves highly differentiated hepatic functions of HepG2 cells.