Wyniki wyszukiwania - Biblioteka Nauki

Nowa wersja platformy, zawierająca wyłącznie zasoby pełnotekstowe, jest już dostępna.
Przejdź na https://bibliotekanauki.pl

Ograniczanie wyników

Znaleziono wyników: 2

Liczba wyników na stronie

Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych: molecular screening

Sortuj według:

Ogranicz wyniki do:

Molecular screening of apple [Malus domestica] cultivars and breeding clones for their resistance to fire blight

100%

Keller-Przybylkowicz S. , Lewandowski M. , Korbin M.

2009

tom 17

nr 2

31-43

Three SCAR and two SSR molecular markers located on Lg 3 and Lg7 were used for early selection of apple genotypes. The purpose of the selection was to find a potential donor for resistance to fire blight (FB) in an apple breeding programme. This study was carried out on 35 breeding clones, and registered cultivars originating from seven countries. They were all characterized as having different levels of susceptibility to FB in field conditions. The number of generated markers varied from 1-2 to 4-5, depending on the genotype. For the majority of the tested genotypes, strong interactions were observed between data concerning plant behaviour in the field and the presence/number of DNA markers. For nine genotypes, however, correlations between phenotypic and molecular study were not found with selected QTLs.

Trzy markery SCAR i 2 markery SSR z grup sprzężeń Lg 3 i 7 wykorzystano do wczesnej selekcji genotypów jabłoni, będących potencjalnymi donorami genów odporno ści na zarazę ogniową. Badania prowadzono na 35 genotypach (klony hodowlane i zarejestrowane odmiany) z siedmiu krajów, różniących się stopniem podatności na zaraz ę ogniową. Liczba amplikonów generowanych na matrycy DNA pochodzących z testowanych genotypów wynosiła od 1-2 do 4-5 fragmentów. Dla większości testowanych roślin obserwowano ścisłe interakcje między liczbą uzyskanych markerów a opisanym przez różnych autorów stopniem podatności tych genotypów na zarazę ogniową w warunkach polowych. Dla 9 genotypów nie znaleziono korelacji między danymi fenotypowymi a wynikami badań molekularnych, opartych na wybranych QTL

Apicomplexa primers amplify Proteromonas (Stramenopiles, Slopalinida, Proteromonadidae) in tissue and blood samples from lizards

100%

Maia J. P. M. C. , Gomez-Diaz E. , Harris D. J.

nr 4

Microscopy has traditionally been the most common method in parasitological studies, but in recent years molecular screening has become increasingly frequent to detect protozoan parasites in a wide range of vertebrate hosts and vectors. During routine molecular screening of apicomplexan parasites in reptiles using the 18S rRNA gene, we have amplified and sequenced Proteromonas parasites from three lizard hosts (less than 1% prevalence). We conducted phylogenetic analysis to confirm the taxonomic position and infer their relationships with other stramenopiles. Although our phylogeny is limited due to scarcity of molecular data on these protists, our results confirm they are closely related to Proteromonas lacertae. Our findings show that unexpected parasites can be amplified from host samples (blood and tissue) using general procedures to detect hemoparasites, and stress that positive PCR amplifications alone should not be considered as definitive proof of infection by particular parasites. Further validation by sequence confirmation and thorough phylogenetic assessment will not only avoid false positives and biased prevalence estimates but also provide valuable information on the biodiversity and phylogenetic relationships of other parasitic organisms. More generally, our results illustrate the perils of general diagnosis protocols in parasitological studies and the need of cross-validation procedures.