Wyniki wyszukiwania - Biblioteka Nauki

1

Magnolol inhibits Streptococcus suis-induced inflammation and ROS formation via TLR2/MAPK/NF-κB signaling in RAW264.7 cells

100%

Wang J. , Sun Y. , Zhang L. , Xu W. , You J. , Lu H. , Song Y. , Wei J. , Li L.

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2

Elements of signalling pathways in the response of Solanum species to Phytophthora infestans

100%

Polkowska-Kowalczyk L. , Olszak K. , Tarwacka J. , Szczegielniak J. , Muszynska G. , Wielgat B.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

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tom 94

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nr 2

3

The effect of concomitant stimulation with cholecystokinin and epidermal growth factor on extracellular signal-regulated kinase (ERK) activity in pancreatic acinar cells

100%

Daniluk J. , Dabrowski A.

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tom 58

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nr 3

EN

The transmission of extracellular proliferation and differentiation signals into their intracellular targets is mediated by a signaling cascade culminating in mitogen-activated protein kinase (MAPK) also known as ERK. In pancreatic acinar cells both cholecystokinin (CCK) and epidermal growth factor (EGF) are known to stimulate ERK. Regulatory interactions among individual receptor-coupled signaling cascades are critically important for establishing cellular responses in the face of multiple stimuli. The aim of our study was to evaluate the effect of concomitant stimulation of G protein-coupled receptors (GPCR) and EGF receptors on ERK activity in isolated pancreatic acinar cells. ERK activity was determined by means of Western-blotting, with the use of the antibody which recognizes active, tyrosine-phosphorylated kinase (pY-ERK). pY-ERK level was strongly elevated by 10 nM CCK-8, 100 µM carbachol (CAR), or 100 nM EGF. The addition of EGF to 60 min-lasting incubations of acini with CCK-8 or CAR caused abrupt decrease of pY-ERK level to 56 and 59% of control, respectively. Similar phenomenon was observed when short stimulation with CCK-8 or CAR was superimposed on the effect of EGF. After the addition of EGF to acini incubated previously with phorbol ester TPA, strong decrease in pY-ERK level was also observed. In conclusion, in pancreatic acinar cells, concomitant stimulation with CCK or CAR and EGF has strong inhibitory effect on ERK cascade. This inhibitory cross-talk may be mediated, at least partially, by protein kinase C (PKC). These mutual inhibitory interactions demonstrate novel mechanism for integration of multiple signals generated by activation of G-protein-coupled and growth factor receptors in pancreatic acinar cells.

4

Peptide inhibitor of MAPK pathway

100%

Fukami Y. , Tokmakov A. A. , Konaka K. , Sato K. I.

Cellular and Molecular Biology Letters

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1998

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tom 03

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nr 3

5

The influence of JNK and P38 MAPK inhibition on IL-12P40 and IL-23 production depending on IL12B promoter polymorphism

84%

Dobreva Z. G. , Stanilova S. A. , Miteva L. D.

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2009

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tom 14

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nr 4

609-621

EN

The interleukin-12p40 gene (IL12B) encodes the p40 polypeptide chain, which, together with p19, composes IL-23. A bi-allelic promoter polymorphism (IL12Bpro) located at −2703 bp of the transcription initiation site has been reported to show associations with IL-12p40 production. To elucidate the dependence of IL-12p40 and IL-23 production on IL12Bpro polymorphism in relation to MAPK signal transduction pathways, we examined the effect of JNK and p38 inhibition on the secretion of these cytokines by stimulated peripheral blood mononuclear cells (PBMC) from healthy donors with 1.1 and 1.2/2.2 IL12Bpro genotypes. Stimulation with LPS and C3bgp resulted in approximately equal IL-12p40 production from PBMC with the 1.1 and 1.2/2.2 genotypes. The inhibition of JNK and p38 before stimulation significantly upregulated IL-12p40 production by PBMC with the 1.1 genotype, but did not influence IL-12p40 production from PBMC with the 1.2/2.2 genotype. Cultures of PBMC with the 1.1 genotype produced significantly more IL-12p40 than PBMC with the 1.2/2.2 genotype after stimulation with PHA. Inhibition of p38 kinase upregulated p40 production only in cultures with the 1.1 genotype. Decreased IL-23 production was observed in C3bgp-stimulated cultures after the inhibition of p38 regardless of the genotype of the tested cells. We concluded that IL-12p40 and IL-23 expression, which is mediated by the p38 and JNK intracellular signaling pathways, is influenced by IL12Bpro polymorphism.

6

Identification, phylogenetic analyses and subcellular localization of protein phosphatases 2c type (PP2Cs) in Populus trichocarpa

84%

Betlinski B. , Vashutina K. , Gawronski P. , Karpinski S.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

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tom 94

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nr 2

7

Expression of some genes in response to cadmium stress in Triticum aestivum

84%

Karimi J. , Mohsenzadeh S.

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tom 63

EN

Heavy metal toxicity has become a universal threat to all life forms, including plants. The main purpose of this study was to identify the gene expression profiling of MAPK, Thioredoxin, and MnSOD genes in wheat seedlings as affected by cadmium treatment. For this experiment, the quantitative Real-Time PCR on RNA isolated from shoots of wheat exposed to CdCl₂ at a concentration of 100 mg/L was used. Results showed that in wheat seedling that exposed to cadmium stress for six days of beginning constant cadmium stress, Thioredoxin gene expression showed a large rise compared with the control sample, MnSOD gene expression increased compared with non-treated wheat seedling at the same times, but unlike the Thioredoxin and MnSOD genes, MAPK gene expression has no significant changes. Of course, it is possible that other times of beginning treatments (instead of six days) cause a change in this gene expression.

8

Thy28 partially prevents apoptosis induction following engagement of membrane immunoglobulin in WEHI-231 B lymphoma cells

84%

Toyota H. , Jiang X. Z. , Asakura H. , Mizuguchi J.

Cellular and Molecular Biology Letters

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2012

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tom 17

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nr 1

EN

Thy28 protein is conserved among plants, bacteria, and mammalian cells. Nuclear Thy28 protein is substantially expressed in testis, liver, and immune cells such as lymphocytes. Lymphocyte apoptosis plays a crucial role in homeostasis and formation of a diverse lymphocyte repertoire. In this study, we examined whether Thy28 affects induction of apoptosis in WEHI-231 B lymphoma cells following engagement of membrane immunoglobulin (mIg). Once they were established, the Thy28-overexpressing WEHI-231 cells showed similar expression levels of IgM and class I major histocompatibility complex (MHC) molecule compared with controls. The Thy28-overexpressing cells were considerably resistant to loss of mitochondrial membrane potential (ΔΨm), caspase-3 activation, and increase in annexin-positive cells upon mIg engagement. These changes were concomitant with an increase in G1 phase associated with upregulation of p27Kip1. The anti-IgM-induced sustained activation of c-Jun N-terminal kinase (JNK), which was associated with late-phase hydrogen peroxide (H2O2) production, was partially reduced in the Thy28-expressing cells relative to controls. Taken together, the data suggest that in WEHI-231 B lymphoma cells, Thy28 regulates mIg-mediated apoptotic events through the JNK-H2O2 activation pathway, concomitant with an accumulation of cells in G1 phase associated with upregulation of p27Kip1 in WEHI-231 B lymphoma cells.

9

Oxidative stress responses are regulated by heat shock factor HSFA4A in Arabidopsis

84%

Perez Salamo I. , Papdi C. , Rigo G. , Zsigmond L. , Vilela B. , Pages M. , Nagy I. , Domoki M. , Darula Z. , Koncz C. , Szabados L.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

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tom 94

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nr 2

10

The role of the oxidative signal-inducible kinase (OXI1) in metal-induced molecular responses in Arabidopsis thaliana

84%

Cuypers A. , Smeets K. , Kelly O. , Hirt H. , Vangronsveld J.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

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tom 94

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nr 2

11

The transcriptional regulation and cell-specific expression of the MAPK-activated protein kinase MK5

84%

Gerits N. , Shiryaev A. , Kostenko S. , Klenow H. , Shiryaeva O. , Johannessen M. , Moens U.

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2009

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tom 14

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nr 4

548-574

EN

The mitogen-activated protein kinase (MAPK) cascades regulate important cellular processes, including growth, differentiation, apoptosis, embryogenesis, motility and gene expression. Although MAPKs mostly appear to be constitutively expressed, the transcript levels of some MAPK-encoding genes increase upon treatment with specific stimuli. This applies to the MAPKactivated protein kinases MK2 and MK3. By contrast, the transcriptional regulation of the related MK5 has not yet been studied. The MK5 promoters of mouse, rat and human contain a plethora of putative transcription factor sites, and the spatio-temporal expression of MK5 suggests inducible transcription of the gene. We examined the transcription pattern of MK5 in different tissues, and studied the kinetics of MK5 expression at the transcriptional and/or translation level in PC12 cells exposed to arsenite, forskolin, KCl, lipopolysaccharide, spermine NONOate, retinoic acid, serum, phorbol ester, temperature shock, and vanadate. Cells exposed to forskolin display a transient increase in MK5 mRNA, despite their unaltered MK5 protein levels. The MK5 promoters of human, mouse and rat contain a cAMP-responsive element that binds the cAMPresponsive element-binding protein (CREB) in vitro. Luciferase reporter constructs containing an 850-base pair human MK5 promoter fragment encompassing the CRE showed a basal activity that was 10-fold higher than the corresponding construct in which the CRE motif was deleted. siRNA-mediated depletion of CREB had no effect on the endogenous MK5 protein levels. Several binding motifs for heat shock factor are dispersed in the mouse and rat promoter, and temperature shock transiently enhanced the MK5 transcript levels. None of the other tested stimuli had an effect on the MK5 mRNA or protein levels. Our results indicate an inducible regulation of MK5 transcription in response to specific stimuli. However, the MK5 protein levels remained unaffected by all the stimuli tested. There is still no explanation for the discrepancy between the increased mRNA and unchanged MK5 protein levels.

12

p-ERK involvement in the neuroprotection exerted by ischemic preconditioning in rat hippocampus subjected to four vessel occlusion

84%

Kovalska M. , Kovalska L. , Mikuskova K. , Tatarkova Z. , Lehotsky J.

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nr 6

13

In silico and in vitro cytotoxic effect of prodigiosin-conjugated silver nanoparticles on liver cancer cells (HepG2)

84%

El-Batal A. I. , El-Hendawy H. H. , Faraag A. H. I.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2017

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tom 98

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nr 3

14

Evidence for differential effects of glucose and cycloheximide on mRNA levels of peroxisome proliferator-activated receptor- (PPAR-) machinery members: Superinduction of PPAR-gamma1 and -gamma2 mRNAs

84%

Rypka M. , Vesely J.

Acta Biochimica Polonica

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2010

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tom 57

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nr 2

EN

Quantitative real-time RT-PCR study was conducted to reveal the effects of normal (5 mmol/l) and high (30 mmol/l) glucose without or with oleate (0.3 mmol/l) on mRNA levels of peroxisome proliferator-activated receptor- (PPAR-)α, -γ1, -γ2, and peroxisome proliferator-activated receptor-γ coactivator- (PGC-)1α and -1β in commercial human hepatoma-derived HepG2 cells maintained under low-serum condition. Significant decrease in PPAR-γ1 and PGC-1α mRNA levels to about 50 % was observed during the first 4 h incubation period. During the next 4 h period, both PPAR-γ1 and PGC-1α mRNAs were partly but significantly restored in high glucose batches. In this period, the presence of the transcriptional inhibitor actinomycin D revealed a significant protective effect of excess glucose on mature PPAR-γ1 and PGC-1α mRNAs. Furthermore, PPAR-γ1 and -γ2 mRNAs were differentially superinduced 1.2-2.5 fold in cells upon the administration of the translational inhibitor cycloheximide. When the cells were co-treated with the combination of cycloheximide and actinomycin D, superinduction was completely suppressed, however. Altogether, the experiments revealed, first, an unexpected protective effect of abundant glucose on PPAR-γ1 and PGC-1α mRNAs in HepG2 cells. Second, we demonstrated cycloheximide-induced, transcription-dependent upregulation of mature PPAR-γ1 and -γ2 mRNAs in HepG2 cells associated with preferential expression of the PPAR-γ2 mRNA variant. The results draw attention to as yet unexplored mechanisms involved in the control of PPAR and PGC genes.

15

Opioids, neutral endopeptidase, its inhibitors and cancer: Is there a relationship among them?

84%

Mizerska-Dudka M. , Kandefer-Szerszen M.

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tom 63

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nr 3

16

Inhibition of p38 kinase mimics survival signal-linked protection against apoptosis in rat cerebellar granule neurons

84%

Nath R. , McGinnis K. , Dutta S. , Shivers B. , Wang K. K. W.

Cellular and Molecular Biology Letters

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2001

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tom 06

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nr 2

17

Time-dependent effect of leptin on renal Naplus, Kplus-ATPase activity

84%

Marciniak A. , Jamroz-Wisniewska A. , Borkowska E. , Beltowski J.

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2005

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tom 52

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nr 4

EN

Leptin, secreted by adipose tissue, is involved in the pathogenesis of arterial hypertension, however, the mechanisms through which leptin increases blood pressure are incompletely elucidated. We investigated the effect of leptin, administered for different time periods, on renal Na+,K+-ATPase activity in the rat. Leptin was infused under anesthesia into the abdominal aorta proximally to the renal arteries for 0.5-3 h. Leptin administered at doses of 1 and 10 μg/min per kg for 30 min decreased the Na+,K+-ATPase activity in the renal medulla. This effect disappeared when the hormone was infused for ≥1 h. Leptin infused for 3 h increased the Na+,K+-ATPase activity in the renal cortex and medulla. The stimulatory effect was abolished by a specific inhibitor of Janus kinases (JAKs), tyrphostin AG490, as well as by an NAD(P)H oxidase inhibitor, apocynin. Leptin increased urinary excretion of hydrogen peroxide (H2O2) between 2 and 3 h of infusion. The effect of leptin on renal Na+,K+-ATPase and urinary H2O2 was augmented by a superoxide dismutase mimetic, tempol, and was abolished by catalase. In addition, infusion of H2O2 for 30 min increased the Na+,K+-ATPase activity. Inhibitors of extracellular signal regulated kinases (ERKs), PD98059 or U0126, prevented Na+,K+-ATPase stimulation by leptin and H2O2. These data indicate that leptin, by acting directly within the kidney, has a delayed stimulatory effect on Na+,K+-ATPase, mediated by JAKs, H2O2 and ERKs. This mechanism may contribute to the abnormal renal Na+ handling in diseases associated with chronic hyperleptinemia such as diabetes and obesity.

18

Recent discoveries in the genetics of malanoma and their therapeutic implications

84%

Marquette A. , Bagot M. , Bensussan A. , Dumaz N.

Archivum Immunologiae et Therapiae Experimentalis

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2007

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tom 55

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nr 6

19

ATP-noncompetitive inhibitors of the c-Jun N-terminal kinase [JNK] subfamily - what can they offer?

84%

Bogoyevitch M. A. , Barr R. K.

Cellular and Molecular Biology Letters

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2005

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tom 10

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nr Suppl.2

20

Screening ERK2 protein and peptide associations by fluorescence anisotropy: insights into ERK2 function and regulation by protein-protein interactions

84%

Callaway K. , Rainey M. , Dalby K. N.

Cellular and Molecular Biology Letters

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2005

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tom 10

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nr Suppl.2