Wyniki wyszukiwania - Biblioteka Nauki

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Differences between C57BL-6 and C57BL-10 inbred mouse strains in chromosomes 2,4,13,17 and 18

100%

Gajewska M. , Rutkowski R. , Lukasiewicz D. , Wirth-Dzieciolowska E.

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tom 20

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nr 3

2

Genetic variation in nine European cattle breeds as determined on the basis of microsatellite markers. I. Within-breed variation

100%

Lubieniecka J. , Grzybowski G. , Lubieniecki K.

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nr 4

3

Preliminary investigations on the polymorphism of microsatellite markers in Mazurian red deer [Cervus elaphus]

100%

Niemczewski C. , Rutkowski J. , Zurkowski M.

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tom 20

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nr 3

4

A microsatellite marker based study of chromosomal regions and gene effects on yield and yield components in maize

86%

Mohammadi S. A. , Prasanna B. M. , Sudan C. , Singh N. N.

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nr 2A

EN

The genetic basis of different traits and the effect of chromosomal regions on the expression of such traits is the primary interest of this study. In the present investigation, the effect of chromosomal regions on yield and its two primary components was studied, and two genetic parameters, additive effect and degree of dominance, were estimated based on a single-locus model using markers with significant effects on the traits (informative markers). Eight inbred lines from diverse geographical regions of India were crossed in all possible pairs, and F1S were evaluated for the yield and yield components in a replicated trial in two environments. Sequential path analysis was employed to find those yield components showing significant direct effects on yield with negligible multicollinearity. The parental lines were profiled using 56 polymorphic SSR (Simple Sequence Repeat) markers covering 10 chromosomes of maize. Stepwise multiple regression analysis was used to determine the informative markers on yield and its primary components detected through path analysis. Fourteen markers were found to have association with chromosomal regions showing significant effects on the total grain yield, 100-grain weight and total number of kernels per ear. Chromosome 1 with four informative markers revealed the highest genic effects on yield and its components. Markers bnlg594 and bnlg1360 on chromosome 10, and bnlg147 on chromosome 1 revealed the highest additive effects on the total grain yield, 100-grain weight and total number of kernels per ear, respectively. For the analyzed traits, overdominance occurred in all the loci and d/a values, varying from 8.60 for 100-grain weight to 1.40 for total grain yield.

5

Fingerprinting of on-farm conserved local tunisian orange cultivars (Citrus sinensis (L.) Osbeck) using microsatellite markers

86%

Abdelaali S. B. , Debbabi O. S. , Abdelaali N. B. , Hajlaoui M. R. , Mars M.

Acta Biologica Cracoviensia. Series Botanica

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2018

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tom 60

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nr 1

6

Application of microsatellite markers developed for arvicoline species in a population genetic study of the root vole Microtus oeconomus

86%

Czajkowska M. , Borkowska A. , Wieczorek M. , Zub K.

Acta Theriologica

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2010

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tom 55

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nr 2

123-128

EN

Using a root vole Microtus oeconomus (Pallas, 1776) population in NE Poland we applied 31 microsatellite markers previously developed for root voles and closely related species, with the aim to improve the population genetic tools in this species. Here we present 16 polymorphic microsatellite markers grouped into four sets suitable for simultaneous amplification and genetically sex identification in M. oeconomus. The number of alleles per locus in 227 individuals varied from 7 to 26 with a low frequency of null alleles, expected heterozygosity ranged from 0.758 to 0.927, and observed heterozygosity from 0.722 to 0.947. Two loci showed significant deviation from Hardy-Weinberg equilibrium (p<0.05) and all loci showed independent inheritance. We expect these markers to be useful for studies of genetic population structure and kinship of M. oeconomus populations.

7

Identification of fecundity gene [FecB] carriers using microsatellite markers and its effect on sheep weight

86%

Nowak Z. , Charon K. M.

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tom 42

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nr 1

8

Chromosome 7q11 controls sperm beat cross frequency [BCF] in mice

86%

Golas A. , Grzmil P. , Muller C. , Styrna J.

Folia Biologica

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2004

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tom 52

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nr 3-4

9

Development of microsatellite markers in rye: map construction

86%

Hackauf B. , Wehling P.

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nr 2

10

Analysis of candidate genes for genotypic diagnosis in the long QT syndrome

86%

Haack B. , Kupka S. , Ebauer M. , Siemiatkowska A. , Pfister M. , Kwiatkowska J. , Erecinski J. , Limon J. , Ochman K. , Blin N.

Journal of Applied Genetics

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2004

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tom 45

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nr 3

11

Porownanie pieciu miar zroznicowania genetycznego polskich odmian pszenicy ocenianych na podstawie danych z analiz markerow mikrosatelitarnych

72%

Bocianowski J. , Stepien L.

Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin

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2006

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nr 242

27-32

12

Does genotype determine habitat preferences? – Studies on forest and field roe deer

72%

Kamieniarz R. , Dobek A. , Molinski K. , Lisowski M. , Szwaczkowski T.

Folia Biologica

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2017

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tom 65

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nr 4

13

Cross-species amplification of microsatellite markers in the invasive spiny-cheek crayfish [Orconectes limosus]: assessment and application

72%

Hulak M. , Kaspar V. , Kozak P. , Buric M. , Filipova L. , Petrusek A.

Journal of Applied Genetics

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2010

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tom 51

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nr 1

73-78

EN

The North American spiny-cheek crayfish, Orconectes limosus (Cambaridae), endangered in its native range, is a widespread invasive species in European waters and conservationally important carrier of crayfish plague. However, its population structure is poorly known, and no informative genetic markers for the species are available. We tested cross-species transfer of microsatellite loci to spiny-cheek crayfish from 5 other crayfish species. Variability of 10 successfully amplifying loci derived from 4 species was then tested in 60 individuals of O. limosus originating from 3 natural populations: the river Danube at Bogyiszló in Hungary, a pond in Starý Klíčov, and the brook Černovický, both in the Czech Republic. The allele number within the populations ranged from 4 to 10 alleles per locus, while heterozygosity levels varied from 0.650 to 0.900 for Ho and from 0.660 to 0.890 for He. No linkage disequilibrium and no null alleles were detected. The selected markers are useful for assessing population structure, intraspecific variation, and paternity studies in spiny-cheek crayfish.

14

Detection of quantitative trait loci affecting carcass traits and internal organs on chromosome 3 in an F2 intercross of Japanese quail

72%

Charati H. , Koshkoiyeh A. E. , Ori R. J. , Moradian H. , Mehrgardi A. A.

Animal Science Papers and Reports

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2014

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tom 32

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nr 4

EN

Molecular technologies based on markers indicating differences among individuals at the DNA level can play an important role in genetic improvement of carcass traits through marker or gene assisted selection. The purpose of this study was to map quantitative trait loci (QTL) of chromosome 3 affecting carcass traits on Japanese quail using microsatellite markers. Two white and wild strains of Japanese quail were crossed reciprocally and the F1 generation was created. The F2 generation was generated by intercrossing F1 birds. Phenotypic data including weights of hot and cold carcasses,carcass parts and internal organs were collected from 422 F2 birds. The total mapping population (472 birds) was genotyped for microsatellite markers. QTL analysis was performed using the least squares regression interval mapping method. Significant QTL were identified for hot and chilled carcass weights, liver weight, head percentage, uropygial gland percentage, intestine percentage, ovary weight, uropygial gland weight, pancreas percentage (0-36 cM with an additive effect),proventriculus percentage, head weight (6-20 cM with a dominance effect), and gizzard percentage (0 cM with an imprinting effect).

15

Characterisation of cross-amplified microsatellite markers in the red-breasted flycatcher Ficedula parva

72%

Mitrus J. , Mitrus C. , Rutkowski R. , Sikora M. , Suchecka E.

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tom 63

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nr 4

EN

We tested 24 microsatellite markers for the red-breasted flycatcher, Ficedula parva, using the primers for the PCR reaction described for other species from genus Ficedula. The amplification efficiency, specificity of the products, and polymorphism of cross-amplified microsatellites were determined based on the genotypes of 65 adult males from a natural population breeding in Białowieża Forest (Eastern Poland). Based on obtained results, we identified 9 highly polymorphic microsatellites, consistently amplifying in majority of individuals. Among those markers between 4 and 26 alleles per locus (mean 15.4) were found and the observed heterozygosity ranged from 0.393 to 0.939. The majority of loci (except for Fhy350 and Fhy458) were in Hardy-Weinberg equilibrium. Accordingly, the values of F IS did not significantly differ from zero 0, except for the locus Fhy350. We suggest that this locus could be loaded with high frequency of null alleles. The polymorphic information content (PIC) for the selected loci set was high and in all cases exceeded 0.82. In addition, we calculated, for each locus, the probability of excluding an improper parent. In majority of loci this parameter distinctly exceeded 0.5. These results demonstrate that tested microsatellite markers can be used to estimate the genetic variability within- and between populations and to establish paternity and parenthood in red-breasted flycatcher populations.

16

Assessment of genetic variability in common whitefish from the catchment area of the Oder river using microsatellite markers

72%

Archem M. , Skuza L. , Kirczuk L. , Domagala J. , Pilecka-Rapacz M. , Czerniawski R.

Acta Biologica

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2017

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tom 24

17

Landscape pattern and genetic structure of a yellow-necked mouse Apodemus flavicollis population in north-eastern Poland

72%

Gortat T. , Gryczynska-Siemiatkowska A. , Rutkowski R. , Kozakiewicz A. , Mikoszewski A. , Kozakiewicz M.

Acta Theriologica

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2010

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tom 55

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nr 2

109-121

EN

Reduced connectivity among local populations inhabiting a spatially heterogeneous landscape may restrict gene flow and thus contribute to diminished genetic variation within a population. The aim of this study was to determine the role of geographic distance and habitat barriers in developing genetic structure of a yellow-necked mouse Apodemus flavicollis (Melchior, 1834) population, taking into consideration the spatial organization of the landscape. A field study was carried out in two plots located in NE Poland that differed considerably in terms of the scale of habitat fragmentation: (1) a continuous forest complex, and (2) a mosaic of smaller forest habitats. The plots were separated by a water barrier comprised of a chain of lakes. DNA samples from a total of 654 individuals were examined by microsatellite analysis (5 loci). The results showed that the yellow-necked mouse population was characterized by a poorly pronounced genetic structure throughout the study area, although the statistical significance of F ST for most location pairs indicated that gene flow in the area was not free. The division of the mouse population into three genetically distinct groups clearly demonstrated the significant role of water bodies as a natural barrier effectively hindering free movement of animals and thus gene flow. Analysis of the genetic structure of the mouse population throughout the study area and also within the distinguished groups indicated that the entire study population may be considered as a single metapopulation. Our results suggest that geographic distance alone is not the predominant factor affecting the genetic structure of population, but in the mosaic landscape the relative isolation of individual forest fragments, and barriers hindering movements of individuals and limiting gene flow among local populations played a much more important role.

18

The cytogenetics and molecular characteristics of a translocated chromosome 1AS.1AL-1DL with a Glu-D1 locus in durum wheat

72%

Blanco A. , Cenci A. , Simeone R. , Gadaleta A. , Pignone D. , Galasso I.

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nr 2A

EN

Wheat quality depends directly on the grain protein content and protein composition. High and low molecular weight glutenin subunits play an important role in determining the visco-elastic properties of gluten. In an attempt to improve the breadmaking quality of hexaploid triticale, a fragment of wheat chromosome 1D, containing the Glu-D1 allele encoding the 5+10 subunits, was translocated to the long arm of chromosome 1A by Lukaszewski and Curtis [1], The 1A.1D translocation chromosome was transferred to tetraploid wheat [2], making the Glu-D1 locus available for the improvement of durum wheat. The goal of this study was to evaluate using cytogenetics and molecular approaches the amount of chromatin introgressed in durum wheat. Fluorescence in situ hybridization with total genomic DNA (GISH) of Aegilops squarrosa L. indicated that the translocated chromosome 1A.1D had a terminal 1DL segment of about 35-40% of the recombinant arm length. Several pairs of microsatellite primers from chromosome 1A and 1D were used to genetically characterize the recombinant chromosome. The mapping data indicated that a 1AL segment, at least 150 cM long, was substituted by a 1DL segment with a minimal length of 72 cM, and that the translocation breakpoint was near the 1A centromeric region. The genetic and physical data highlight a substantial discrepancy between the recombinational and physical map distances. We are using a targeted strategy via the Ph pairing manipulation system to generate smali intercalary 1D chromosome segments in a durum wheat background.

19

Ocena wybranych metod izolacji DNA pod wzgledem ich przydatnosci w hodowli pszenicy

72%

Pietrusinska A. , Czembor P. C.

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nr 244

59-68

20

Genetic variation in nine European cattle breeds as determined on the basis of microsatellite markers. III. Genetic integrity of the Polish Red cattle included in the breed preservation programme

72%

Grzybowski G. , Prusak B.

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tom 22

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nr 1

EN

On the basis of DNA microsatellite polymorphism at 26 loci tested within the European Concerted Action AIRE 2066 for the Analysis of Genetic Diversity to Preserve Future Breeding Options, a determination was made of the genetic structure of 147 Polish Red (PR) cattle, included in the National Rare Livestock Breeds Preservation Programme (NRLBPP). The examined PR cattle population was characterized by a high genetic variation (a total of 193 alleles identified, Ho = 0.695, He = 0.703,mean number of alleles per locus = 7.4). An analysis of the genetic distance (Dps), including information on the presence (or absence) in the genome of alleles specific for the breed, confirmed that 80% of PR animals included in the NRLBPP comprised a separate genetic group, differing from populations of other European cattle breeds. The results show the uniqueness of the gene pool of PR cattle included in the NRLBPP. Despite the crossing with other breeds widely applied in the past, the present PR material does, to a considerable degree, remain genetically distinct. Thus, it is anticipated that basing on the existing preserved population the reconstruction of a pure, or almost pure PR cattle can be achieved.

PL

Na podstawie polimorfizmu mikrosatelitów DNA w 26 loci wybranych do zunifikowanych analiz w europejskim programie ochrony zasobów genetycznych (European Concerted Action AIRE2066 for the Analysis of Genetic Diversity to Preserve Future Breeding Options), określono strukturę genetyczną bydła pc (PR) objętego programem hodowli zachowawczej oraz umiejscowiono ten materiał w drzewie filogenetycznym bydła. Wykazano, że na tle ras europejskich, badaną populację bydła pc charakteryzuje wysoka zmienność genetyczna (ogółem zidentyfikowano 193 allele, Ho = 0,695, He = 0,703, średnia liczba alleli w locus = 7,4). Analizy dystansu genetycznego (Dps), w których wykorzystano informacje dotyczące obecności (lub nieobecności) w genomie tzw. alleli specyficznych dla rasy dowodzą, że 80% osobników rasy pc tworzy odrębną grupę genetyczną, odbiegającą od innych populacji bydła europejskiego. Jedynie mała grupa bydła pc grupuje się w drzewie filogenetycznym wspólnie z osobnikami rasy angler lub niemiecki simental. Wyniki wskazują na unikalność puli genów bydła pc objętego programem hodowli zachowawczej.Mimo licznych krzyżowań z innymi rasami, materiał ten w znacznym stopniu zachował swą rasową odrębność. Wydaje się więc możliwe, że korzystając z utworzonej stawki zwierząt (grupa północna i grupa południowa), uda się odtworzyć czystorasową bądź zbliżoną do czystorasowej, populację tego bydła.