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EN
The effect of inoculating single cultures of plant growth-promoting rhizobacteria (PGPR) on development of white beans seedling (Phaseolus vulgaris) was analyzed. Five PGPR were isolated from the rhizosphere of Okra plants and were assessed for abilities to solubilise phosphate and produce indole acetic acid (IAA). The phosphate solubilising index ranged from 6-10 while the concentration of IAA ranged from 17.48mg/l to 27.43mg/l. Serratia sp. produced the highest concentration of IAA (27.43mg/l) and had the highest solubilisation index (10mm). Bacillus sp. produced the least amount of IAA (17.48mg/l) while Staphylococcus sp. had the least solubilisation index (6mm). The highest percentage germination of 83.3% was observed in the seedling inoculated with Bacillus sp. The effect of PGPR on root and shoot elongation was studied hydroponically for 7 days. Significant increases (P< 0.05) in root elongation were observed.. The highest seedling root length (18.47cm) and shoot length (19.17cm) were observed with inoculation of Staphylococcus sp. and Bacillus sp. respectively. The use of these bacteria as bio-inoculants could be a sustainable practice to facilitate nutrient supply to white beans seedlings.
EN
Two inoculums: Effective Microorganisms (EM) specimen available on the market and microbiological BAF1 inoculum, were applied in the experiment. The plants were cultivated in the growth chamber equipped with shelves with fluorescent or LED lamps. The highest number of inflorescences was under the influence of white color of light emitted by fluorescent lamps and blue color of light emitted by LED lamps, especially after application of BAF1 inoculum. Irrespective of microbiological inoculum, no significant effect of the color of light and type of lamps on such traits as height of leaves layer, number of leaves, greening index of leaves (SPAD) and length of inflorescences, was found. The white color light emitted by fluorescent lamps stimulated actinobacteria multiplication, especially after EM application. Regardless of the inoculum application, it was the blue color light emitted by LED lamps that stimulated the multiplication of moldy fungi. After the use of fluorescent lamps, the increase in dehydrogenase activity was observed, especially after the application of BAF1 inoculum. The activity of acid phosphatase was stimulated by blue and white+blue light emitted by LED lamps. The increase in the activity of urease was observed under fluorescent lamps emitting the green, blue and white color of light, after the application of EM.
PL
Praca przedstawia charakterystykę mikrobiologiczną osadu ściekowego kompostowanego w warunkach laboratoryjnych wraz ze słomą i trocinami, w pojemnikach o pojemności 5000 cm3. Celem przeprowadzonych badań było określenie wpływu kompostowanego osadu ściekowego, inokulowanego wybranymi szczepionkami mikroorganizmów na dynamikę wzrostu i rozwoju wybranych grup drobnoustrojów oraz przeżywalność drobnoustrojów z rodziny Enterobacteriaceae. Analizy mikrobiologiczne przeprowadzono na wybiórczych podłożach, metodą płytkową, oznaczając liczbę heterotroficznych bakterii mezofilnych i grzybów pleśniowych oraz mikroorganizmów z rodziny Enterobacteriaceae. Uzyskane rezultaty badań wykazały, że proces kompostowania nie wyeliminował bakterii z rodziny Enterobacteriaceae. Natomiast dynamika wzrostu i rozwoju liczby bakterii heterotroficznych i grzybów pleśniowych przebiegała zgodnie z następstwem zmian faz kompostowania. Odpowiednio dla obu grup mikroorganizmów następowała redukcja ich liczby wraz ze wzrostem temperatury podczas fazy termofilnej, a następnie w fazie schładzania i dojrzewania kompostu ponowne ich namnażanie. Inokulacja kompostów szczepionkami mikrobiologicznymi nie przynosiła efektu w postaci intensyfikacji przebiegu procesu kompostowania. Aktywność dehydrogenaz wraz ze wzrostem temperatury podczas procesu kompostowania zmniejszała się. Natomiast w fazie schładzania i dojrzewania kompostu zwiększała się. We wszystkich kompostach występowała dodatnia korelacja pomiędzy liczebnością badanych grup drobnoustrojów, a poziomem aktywności dehydrogenaz.
EN
The presented paper shows a microbiological characteristics of sewage sludge composted in laboratory conditions with straw and sawdust, placed in containers of 5000 cm3. The objective of studies was the determination of the effect of composted sewage sludge inoculated with selected microorganisms inoculum on the dynamics of growth and development of selected microorganism groups and on the survival of microorganisms from the Enterobacteriaceae family. Microbiological analyses were performed on selective medium with the plate method to determine the number of heterotrophic mezophilic bacteria and molds, as well as microorganisms from Enterobacteriaceae family. Obtained results of studies showed that the composting process did not eliminated the bacteria of the Enterobacteriaceae family. On the other hand, the dynamics of growth and development of the number of the heterotrophic mezophilic bacteria and moulds showed a course running according to the regular sequence of changes in the composting phases. In both groups, the number of microorganisms was gradually reduced with the temperature increase during the thermophilous phase and subsequently, in the phases of compost cooling and ripening, a repeated proliferation of the microorganisms occurred. The inoculation of compost with microbiological inoculum did not intensify the course of the composting process. Dehydrogenase activity in the composting process decreased when the temperature increased during the composting process. On the other hand, in the phases of compost cooling and ripening, the enzymatic activity increased. In all composts, a positive correlation occurred between the number of the studied microorganism groups and dehydrogenase activity.
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