Wyniki wyszukiwania - Biblioteka Nauki

Nowa wersja platformy, zawierająca wyłącznie zasoby pełnotekstowe, jest już dostępna.
Przejdź na https://bibliotekanauki.pl

Ograniczanie wyników

2 Medycyna Weterynaryjna

Znaleziono wyników: 2

Liczba wyników na stronie

Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych: metoda PCR-REA

Sortuj według:

Ogranicz wyniki do:

Wewnatrzgatunkowe roznicowanie szczepow Malassezia pachydermatis z wykorzystaniem PCR-REA

100%

Nowakiewicz A. , Ziolkowska G.

tom 64

nr 06

822-827

The aim of the study was to make an attempt at showing the intraspecies heterogenecity of Malassezia pachydermatis strains with regards to their origin (strains isolated from healthy dogs and with otitis externa symptoms). The study included 41 strains of Malassezia pachydermatis species isolated in a pure culture from dogs with clinical otitis externa symptoms (n = 20), clinically healthy dogs (n = 20) and a reference strain, M. pachydermatis (CBS7925). In order to isolate the genetic material from the fungal cells, the following four procedures were selected: mechanical, enzymatic, thermal and chemical. Considering the yield and repeatability of a method for the genomic DNA extraction, a mechanical method was applied. The genetic material research of each strain was performed according to PCR-REA technique with the amplification of three genome regions: ITS, LSU rRNA and a gene encoding beta-tubuline. The ITS and LSU rRNA regions were amplified employing the standard PCR reagents, whereas the region coding beta-tubuline with the so called touch down. The obtained amplification products were subjected to restrictive analysis by means of the following enzymes: EcoRI, Ncol, Hinfl, Alul, and Eco881 (Aval). The performed investigations made it possible to reveal the genotypic differentiation within M.pachydermatis species as well as some correlation between a genotypic profile and the origin of a strain (from healthy animals or with otitis externa symptoms), which may imply the existence of genetic conditioning of the Malassezia strains’ pathogenicity.

Izolacja lipidozaleznych szczepow Malassezia od psow i kotow

84%

Ziolkowska G. , Nowakiewicz A.

tom 62

nr 08

913-916

The study investigated 180 clinically healthy dogs and 35 cats with symptoms of otitis externa. 96 strains of Malassezia were isolated, including 13.5% (13 strains) of the lipid-dependent species, and the remainder was classified as M. pachydermatis. Ten lipophilic isolates came from diseased animals, two of which were isolated from dogs. M. globosa (5 strains), M. sympodialis (5 strains), M. furfur (one strain) were isolated within the lipophilic strain pool by using phenotype classification and two isolate species remained unidentified. Genotype identification was performed by PCR-REA (ITS, 26S,Bt) and biochemical identification results for all M. sympodialis and M. globosa strains were confirmed. The M. furfur strain and two isolates of an unrecognizable species were reclassified to M. pachydermatis. Isolating lipid-dependent Malassezia strains from animals having otitis externa is a unique phenomena, and, it seems, that this is the first time in which their isolation and identification from dogs by the use of molecular biology techniques has been described.