Wyniki wyszukiwania - Biblioteka Nauki

1

SOCS3 is epigenetically up-regulated in steroid resistant nephrotic children

100%

Zaorska K. , Zawierucha P. , Ostalska-Nowicka D. , Nowicki M.

Acta Biochimica Polonica

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2016

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tom 63

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nr 1

131-138

EN

Background. The mechanism of steroid resistance in children with the nephrotic syndrome is yet unknown. About 20% of patients demonstrate steroid unresponsiveness and progress to end stage renal disease. Aberrant SOCS3 and SOCS5 expression in steroid resistant and sensitive patients has previously been demonstrated. Here, we investigate genetic and epigenetic mechanisms of regulation of SOCS3 and SOCS5 transcription in nephrotic children. Methods. 76 patients with the nephrotic syndrome (40 steroid resistant and 36 steroid sensitive) and 33 matched controls were included in this study. We performed genotyping of a total of 34 single nucleotide polymorphisms for SOCS3 and SOCS5 promoters and evaluated their methylation status using MS-PCR and QMSP methods. Results. Steroid resistant patients had a significantly lower methylation of one region of SOCS3 promoter in comparison with steroid sensitive patients and controls (p < 0.0001). However, the relative methylation level in the steroid sensitive patients and controls differed significantly even before the first steroid dose (p = 0.001758). Other SOCS3 and SOCS5 promoter regions displayed no differences in methylation or were fully methylated/unmethylated in all study groups, showing site-specific methylation. The allele and genotype distribution for SOCS3 and SOCS5 markers did not differ statistically between the groups. Conclusions. We demonstrate an epigenetic mechanism of SOCS3 up-regulation in steroid resistant children with the nephrotic syndrome. The assessment of methylation/unmethylation of SOCS3 promoter might be an early marker for steroid responsiveness in NS patients.

2

Introduction of Selenium to Heterocyclic Compounds : Part VII: Synthesis of 3-Alkyl-5-benzylidene- and 3-Alkyl-5-cinnamylidene-2-selenorhodanines

100%

Tejchman W. , Korohoda M.

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1999

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tom Vol. 73, nr 8

1315-1322

EN

Five new 3-alkyl-5-benzylidene- and five new 3-alkyl-5-cinnamylidene-2-selenorhodanines were obtained by treatment of methylation products of appropriate rhodanines with H2Se. The stability of 2-thiazolinium salts with SCH3 or RNCH3 group formed during methylation is determined by substituents at C-5 and N-3 atoms.

3

A novel repetitive DNA sequence in lemon [Citrus limon [L.] Burm.] and related species

100%

De Felice B. , Wilson R. R. , Ciarmiello L. , Conicella C.

Journal of Applied Genetics

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2004

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tom 45

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nr 3

4

Supplementary material : Lei et al., ABCbot 63(1) 2021, LaCl3 induces genomic DNA instability and increases DNA methylation levels in wheat roots

88%

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2021

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tom 63

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nr 1

5

Genetyka zachowania: co wnosi do wiedzy o człowieku?

88%

Oniszczenko W.

Polskie Forum Psychologiczne

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2017

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tom XXII

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nr 1

5-19

EN

The aim of this article is to highlight the achievements of human behavioural genetics. It begins with a brief overview of the field of contemporary human behaviour genetics. Then, the general principles of behavioural genetics, research methods used, the concept of heritability and areas of rapid advancement in the field are identified. While classical twin studies have been a powerful tool to find heritability or the genetic correlation between different human behaviours, new tools are now available to help identify the genes responsible for individual differences. In particular, association studies and DNA methylation studies are crucial to advancing knowledge on the genetic basis of human behaviour as well as on the epigenetic factors that mediate genetic and environmental effects on behaviour. Several results on the heritability of human behaviour, relationships between genetic polymorphisms and behaviour as well as the consequences of DNA methylation are reported in this article.

6

Using capillary electrophoresis to study methylation effect on RNA-peptide interaction.

88%

Mucha P. , Szyk A. , Rekowski P. , Agris P. F.

Acta Biochimica Polonica

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2003

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tom 50

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nr 3

857-864

EN

Methylation of RNA and proteins is one of a broad spectrum of post-transcriptional/translational mechanisms of gene expression regulation. Its functional signification is only beginning to be understood. A sensitive capillary electrophoresis mobility shift assay (CEMSA) for qualitative study of the methylation effect on biomolecules interaction is presented. Two RNA-peptide systems were chosen for the study. The first one consists of a 17-nucleotide analogue (+27-+43) of the yeast tRNAPhe anticodon stem and loop domain (ASLPhe) containing three of the five naturally occurring modifications (2'-O-methylcytidine (Cm32), 2'-O-methylguanine (Gm34) and 5-methylcytidine (m5C40)) (ASLPhe-Cm32,Gm34,m5C40) and a 15-amino-acid peptide (named tF2 : Ser1-Ile-Ser-Pro-Trp5-Gly-Phe-Ser-Gly-Leu10-Leu- Arg-Trp-Ser-Tyr15) selected from a random phage display library (RPL). A peptide-concentration-dependent formation of an RNA-peptide complex was clearly observable by CEMSA. In the presence of the peptide the capillary electrophoresis (CE) peak for triply methylated ASLPhe shifted from 18.16 to 20.90 min. Formation of the complex was not observed when an unmethylated version of ASLPhe was used. The second system studied consisted of the (+18)-(+44) fragment of the trans-activation response element of human immunodeficiency virus type 1 (TAR RNA HIV-1) and a 9-amino-acid peptide of the trans-activator of transcription protein (Tat HIV-1) Tat(49-57)-NH2 (named Tat1 : Arg49-Lys-Lys-Arg52-Arg-Gln-Arg-Arg- Arg57-NH2). In the presence of Tat(49-57)-NH2 a significant shift of migration time of TAR from 18.66 min to 20.12 min was observed. Methylation of a residue Arg52→Arg(Me)2, crucial for TAR binding, strongly disrupted formation of the complex. Only at a high micromolar peptide concentration a poorly shaped, broad peak of the complex was observed. CE was found to be an efficient and sensitive method for the analysis of methylation effects on interaction of biomolecules.

7

Using capillary electrophoresis to study methylation effect on RNA-peptide interaction

88%

Mucha P. , Szyk A. , Rekowski P. , Agris P. F.

Acta Biochimica Polonica

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2003

|

tom 50

|

nr 3

EN

Methylation of RNA and proteins is one of a broad spectrum of post-trans- criptional/translational mechanisms of gene expression regulation. Its functional signification is only beginning to be understood. A sensitive capillary electrophoresis mobility shift assay (CEMSA) for qualitative study of the methylation effect on biomolecules interaction is presented. Two RNA-peptide systems were chosen for the study. The first one consists of a 17-nucleotide analogue (+27-+43) of the yeast tRNA Phe anticodon stem and loop domain (ASL Phe) containing three of the five naturally occurring modifications (2'-O-methylcytidine (Cm32), 2'-O-methylguanine (Gm34) and 5-methylcytidine (m5 C40)) (ASL Phe -Cm32,Gm34,m5 C40) and a 15-amino-acid peptide (named tF 2: Ser1 -Ile-Ser-Pro-Trp5 -Gly-Phe-Ser-Gly-Leu10 -Leu- Arg-Trp-Ser-Tyr15 ) selected from a random phage display library (RPL). A pep- tide-concentration-dependent formation of an RNA-peptide complex was clearly observable by CEMSA. In the presence of the peptide the capillary electrophoresis (CE) peak for triply methylated ASL Phe shifted from 18.16 to 20.90 min. Formation of the complex was not observed when an unmethylated version of ASL Phe was used. The second system studied consisted of the (+18)-(+44) fragment of the trans-activation response element of human immunodeficiency virus type 1 (TAR RNA HIV-1) and a 9-amino-acid peptide of the trans-activator of transcription protein (Tat HIV-1) Tat(49–57)-NH2 (named Tat1: Arg49-Lys-Lys-Arg52-Arg-Gln-Arg-Arg- Arg57-NH2). In the presence of Tat(49–57)-NH2 a significant shift of migration time of TAR from 18.66 min to 20.12 min was observed. Methylation of a residue Arg52->Arg(Me)2, crucial for TAR binding, strongly disrupted formation of the complex. Only at a high micromolar peptide concentration a poorly shaped, broad peak of the complex was observed. CE was found to be an efficient and sensitive method for the analysis of methylation effects on interaction of biomolecules.

8

Genomic imprinting in mammals

88%

Wrzeska M. , Rejduch B.

Journal of Applied Genetics

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2004

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tom 45

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nr 4

9

Rumex acetosa Y chromosomes: constitutive or facultative heterochromatin?

88%

Mosiolek M. , Pasierbek P. , Malarz J. , Mos M. , Joachimiak A. J.

Folia Histochemica et Cytobiologica

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2005

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tom 43

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nr 3

10

Sperm epigenetic profile and risk of cancer

75%

Wdowiak A.

Journal of Pre-Clinical and Clinical Research

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2014

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tom 08

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nr 2

EN

Introduction and objective. The integrity, stability and composition of sperm chromatin are of great importance in the fertilizing potential of male gametes and their capacity to support normal embryonic development. In this study, the author presents the current state of knowledge about the sperm epigenetic profile and risk of cancer. Abbreviated description of the state of knowledge. The obtaining of pregnancy and the state of health of the baby depends on the quality of the genetic material of both the female and the male. Health behaviours and environmental factors directly affect the quality of sperm, as well as the human egg cell and, consequently, on the reproductive capabilities, the course of pregnancy and the state of the newborn. There exist two thoroughly investigated epigenetic modifications: DNA methylation and histone modifications. The process of DNA methylation can be also a fundamental factor contributing to the development of cancer, where epigenotype undergoes significant modifications. When considering numerous DNA aberrations in the male gamete, the most commonly encountered is DNA fragmentation, particularly in infertile subjects. Surprisingly, an intracytoplasmatic sperm injection study of mice oocytes, using spermatozoa with a high DNA Fragmentation Index (DFI), revealed that a considerable percentage of adults born as a result of this method, showed a significant increase in the incidence of abnormal behavioural tests, malformations, cancer and signs of premature aging. Summary. The issue of assisted procreation raises the need to look for an appropriate treatment for males with sperm chromatin abnormalities. As a result, the fight against smoking addiction becomes the obvious necessity. Moreover, the reasonable solution nowadays seems to be supplementation with micronutrients and folic acid. It has been proved that the process of DNA fragmentation is a phenomenon that intensifies over time. Therefore, there should be a pursuance for, as close as possible, to the moment of ejaculation, application of semen to reproductive techniques. Finally, epigenetic changes are suspected of being one of the factors responsible for the deterioration of male sperm parameters observed in recent decades.

11

LaCl3 induces genomic DNA instability and increases DNA methylation levels in wheat roots

75%

Lei X. , Ma K. , Zhang F.

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2021

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tom 63

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nr 1

12

Methylation of the arginine-glycine-rich region in the fragile X mental retardation protein FMRP differentially affects RNA binding

75%

Denman R. B.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr 3

EN

The C-terminal end of the fragile X mental retardation protein contains a stretch of amino acid residues that are enriched in arginine and glycine. Recent studies using recombinant FMRPs have demonstrated that this region participates in RNA binding in vitro, with calculated KdS ranging from 1-10 nM depending on the RNA. It is known that other arginine glycine-rich proteins are subject to site-specific methylation by protein arginine methyltransferases (PRMTs) that are particularly abundant in most cells. We have demonstrated that the interaction of homoribopolymer mimetic RNAs with human FMRP (hFMRP) made in PRMT-containing cell-free lysates is more sensitive to increasing salt concentrations than recombinant hFMRP expressed in bacteria. We have also shown that blocking methylation with adenosine-2', 3'-dialdehyde (AdOx) alters homoribopolymer binding and hFMRP target mRNA binding; both increases and decreases are observed as a function of methylation. These data suggest that changes in PRMT activity that occur during development, or arise via signal transduction may be a means of regulating the binding of hFMRP to mRNA in vivo.

13

Novel procedure for the detection of 5-methylcytosine

75%

Radlinska M. , Skowronek K.

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1998

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tom 47

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nr 4

14

Methylation-sensitive restriction endonuclease digestion patterns revealed in Vicia faba L. chromosomes by in situ nick-translation

75%

Olszewska M. J. , Gernand D. , Sakowicz T.

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nr 4

15

Prognostic significance of DAPK and RASSF1A promoter hypermethylation in non-small cell lung cancer [NSCLC]

63%

Niklinska W. , Naumnik W. , Sulewska A. , Kozlowski M. , Pankiewicz W. , Milewski R.

Folia Histochemica et Cytobiologica

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2009

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tom 47

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nr 2

275-280

16

Epigenetic basis of molecular changes in animal cells with particular regard to embryonic development - a review

63%

Romanek J.

Annals of Animal Science

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2013

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tom 13

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nr 4

17

TGF-beta1 immunohistochemistry and promoter methylation in chronic renal failure rats treated with uremic clearance granules

63%

Miao X.-H. , Wang C.-G. , Hu B.-Q. , Li A. , Chen C.-B. , Song W.-Q.

Folia Histochemica et Cytobiologica

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2010

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tom 48

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nr 2

284-291

18

Comparison of epigenetic changes induced by particular phytohormones and oligonucleotide sequences in flax

63%

Dzialo M. , Zuk M. , Szopa-Skorkowski J.

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2015

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tom 96

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nr 1

19

Wybrane procesy z udziałem metanu jako czynnika metylującego

63%

Czechowicz D.

Przemysł Chemiczny

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2005

|

tom T. 84, nr 6

454-457

PL

Dokonano przeglądu wybranych procesów metylowania z udziałem metanu jako czynnika metylującego.

EN

A review with 41 refs. covering oxidative coupling of CH4, oxidative methylation of toluene to ethylbenzene and styrene, and oxidative methylation of acetonitrile to acrylonitrile.

20

A stable density approach to probe selection for a custom aCGH design

63%

Gambin T. , Stankiewicz P. , Gambin A.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2011

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tom 92

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nr 3