Wyniki wyszukiwania - Biblioteka Nauki

1

Evaluation of phytochemical constituents of Methanol extract of Moringa oleifera Lam. whole leaf by Gas Chromatography-Mass Spectrometry and Fourier transform infrared spectroscopy analysis

100%

Okechukwu V. U. , Eze S. O. , Omokpariola D. O. , Okereke J. C.

World News of Natural Sciences

|

2021

|

tom 37

18-30

EN

The research study evaluated the phytochemical constituents of Methanol extracts of Moringa oleifera whole leaf by Gas Chromatography-Mass Spectrometry (GC-MS) and Fourier transform infrared spectroscopy (FT/IR) analysis. The leaves was washed, air dried for 2 weeks, then ground into a fine powder and extracted using methanol by maceration for 24 hours using standard procedures. After the contact time elapsed, the solvent filtered to recover the extract. The functional groups and the chemical constituents of the methanol extract of Moringa oleifera leaves investigated using Fourier transform infrared spectroscopy (FT/IR) and Gas Chromatography-Mass Spectrometry (GC-MS) respectively. The FT/IR analysis confirmed the presence of O-H, C=C, C-H, C-O, CH3 and C=C-H bond stretching functional groups, which indicates that the substance is an aliphatic alcohol, ester, aldehyde and carboxylic acid. However, the fingerprint region had a pattern that is specific for every molecule, the presence of -OH function and N-O stretch; suggest that or alcohols and nitrogen or aromatic or aliphatic phenols containing molecules are major components of the Moringa oleifera leaf studied. GC-MS analysis of the extract reveals the identification of twenty compounds, in which two compounds were identified in each peak. N,N'-Pentamethylenebis[s-3-aminopropyl thiosulfuric acid and 2-Myristynoyl pantetheine (100%), 2-Myristynoyl pantetheine and Deoxyspergualin (92.05%), 5-Octadecenal and 9-Hexadecenoic acid (27.94%). N,N'-Pentamethylenebis[s-3-aminopropyl thiosulfuric acid and Pentetic Acid are the major phytoconstituents. Most of the compounds in the list are bioactive and possess medicinal properties, which further justify the application of Moringa oleifera traditional plant in the discovery of novel therapeutics.

2

In vitro antibacterial activity of Eclipta alba (L.) Hassk

86%

Selvamani S. , Balamurugan S.

International Letters of Natural Sciences

|

2014

|

tom 16

EN

The antibacterial activity of Eclipta alba against three gram positive and five gram negative bacterial strains was investigated. The fresh whole plants were collected from Chidambaram, Tamil Nadu. Petroleum ether, chloroform, ethyl acetate, acetone, methanol and aqueous extracts at different concentrations (1, 2, 5, 10 mg/ml) were used to investigate the antibacterial activity. NCCL standards were strictly followed to perform antibacterial disc susceptibility test using disc diffusion method. The extracts showed varying degree of inhibitory potential against all the tested bacteria. Methanol extract of the plant had higher inhibitory action against Staphylococcus aureus, Bacillus subtilis, Proteus mirabilis and Pseudomonas fluroescens.

3

Phenolic acids in extracts obtained from the flowering herbs of Cirsium vulgare (Savi) Ten. growing in Poland

86%

Kozyra M. , Glowniak K.

Acta Societatis Botanicorum Poloniae

|

2013

|

tom 82

|

nr 4

EN

In this work the phenolic acids in the methanol extract from the flowering herbs of Cirsium vulgare (Savi) Ten. growing in Poland were isolated and identified. The samples containing free phenolic acids and those released after acid and alkaline hydrolyses were investigated by 2D TLC on cellulose. After purification by SPE, samples were also analyzed by RP-HPLC. Six phenolic acids such as gallic, protokatechuic, gentisic, hydrobenzoic, vanillic and caffeic acids were detected in the fraction of free phenolic acids of the methanol extract, irrespectively of the method used.

4

Antibacterial activity of Ulva fasciata against multidrug resistant bacterial strains

86%

Chandrasekaran M. , Venkatesalu V. , Raj G. A. , Krishnamoorthy S.

International Letters of Natural Sciences

|

2014

|

tom 14

EN

The present study was conducted to evaluate the antibacterial activity of different organic solvent increasing polarity viz., hexane, chloroform, ethyl acetate, acetone and methanol extracts of Ulva fasciata (Chlorophyceae) were collected from Kanniyakummari, Gulf of Mannar biosphere Reserve, Tamilnadu, India. Marine green algae extracts of U. fasciata against multi-drug resistant standard and clinical bacterial strains viz., Bacillus subtilis, Streptococcus pyogenes, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Salmonella typhimurium, Vibrio cholerae, Shigella flexneri, Proteus mirabilis and P. vulgaris. The ethyl acetate extracts of U. fasciata showed highest antibacterial activity against all the bacterial strains tested. The mean zone of inhibition produced by the extracts in disc diffusion assays were ranged from 7.1 mm to 15.0 mm. The Minimum Inhibitory Concentrations (MIC) were between 125 μg/ml and 500 μg/ml, while the Minimum Bactericidal Concentrations (MBC) were between 250 μg/ml and 1000 μg/ml. The highest mean of zone inhibition (15.0 mm) and lowest MIC (125 μg/ml) and MBC (250 μg/ml) values were observed in ethyl acetate extract of U. fasciata against B. subtilis. The ethyl acetate extract of the U. fasciata showed the presence of phytochemicals, terpenoids, tannins and phenolic compounds in U. fasciata than the other solvents extracts. The present results of the ethyl acetate extract of U. fasciata can be used as an antibacterial substance for the treatment of multi drug resistant bacterial infections.

5

Phytochemical investigation of the roots of Janakia arayalpatra Joseph et Chandrasekharan

86%

Chacko S. , Sethuraman M. G. , George V.

Herba Polonica

|

2000

|

tom 46

|

nr 4

PL

Z wyciągu heksanowego, chloroformowego i metanolowego z korzeni Janakia arayalpatra wyizolowano 8 związków. Związki te zostały określone jako octan a-amyryny, aldehyd 4-meto- ksy-salicylowy, aldehyd 3-hydroksy-anyżowy, magnifikol (12,20(29)-lupadien-3-ol), ß-sitoste- rol, naryngenina, kemferol, aromadendryna.

EN

From the hexane, chloroform and methanol extracts of the roots of Janakia arayalpatra eight compounds have been isolated. These compounds have been characterized as a-amyrin acetate, 4-methoxy salicylaldehyde, magnificol (12,20(29)-lupadien-3-ol), ß-sitosterol, 3-hydroxy- -p-anisaldehyde, naringenin, kaempferol and aromadendrin.

6

Cucumis sativus and Cucurbita maxima extract attenuate diabetes-induced hepatic and pancreatic injury in a rat model

72%

Atta A. H. , Saad S. A. , Atta S. A. , Mouneir S. M. , Nasr S. M. , Desouky H. M. , Shaker H. M.

Journal of Physiology and Pharmacology

|

2020

|

tom 71

|

nr 4

7

Antioxidant activities of the essential oil and extracts of Biebersteinia multifida DC

72%

Amiri H.

Herba Polonica

|

2009

|

tom 55

|

nr 2

59-68

EN

In present study the essential oils composition and antioxidant activities of the essential oils as well as methanolic extracts of leaves, fruits and roots of Biebersteinia multifida were evaluated. The GC-MS analysis of the essential oils resulted in the identification of 36 compounds. Thymol (16.5–38.4%), β-caryophyllene (9.8–15.5%), 1,8-cineol (5.8–18.4%), α-pinene (0.9–14.3%) and β-pinene (2.3–12.4%) were the main components. The samples were subjected to screening for their possible antioxidant activity by using DPPH and β-carotene-linoleic acid assay. In the first case, the radical scavenging activity of the essential oil and methanolic extract of fruits were superior to all other essential oils and extracts (IC50=16.7). In the case of the linoleic acid system, oxidation of the linoleic acid was effectively inhibited by essential oils and extracts of different parts. The fruit extracts showed 95.4±2.15% inhibition, which is comparible to synthetic antioxidant BHT, curcumine and ascorbic acid.

PL

W niniejszej pracy badano skład i właściwości przeciwutleniające olejków eterycznych, a także wyciągów metanolowych z liści, owoców i korzeni Biebersteinia multifida. Analiza GC/MS olejków eterycznych pozwoliła na identyfikację 36 składników. Główne z nich to tymol (16,5–38,4%), β-kariofilen (9,8–15,5%), 1,8-cyneol (5,8–18,4%), α-pinen (0,9–14,3%) i β-pinen (2,3–12,4%). Próbki zostały poddane skriningowi na ich możliwe właściwości przeciwutleniające za pomocą testu DPPH i testu β-karoten-kwas linolenowy. W teście DPPH aktywność jako zmiatacza wolnych rodników dla olejku eterycznego i wyciągu alkoholowego z owoców była wyższa niż dla innych olejków eterycznych i wyciągów (IC50=16,7). W przypadku testu z kwasem linolenowym utlenienie kwasu linolenowego zostało efektywnie zahamowane przez olejki eteryczne i wyciągi z różnych części rośliny. Wyciągi z owoców powodowały zahamowanie o 95,4±2,15%, a więc porównywalne z syntetycznym antyoksydantem BHT, kurkuminą i kwasem askorbowym.

8

Screening of different leaf extracts of Cassia fistula Linn for investigation of hypolipidemic activity in two different rat models

72%

Sutar G. V. , Dass K. , Einstein J. W.

International Letters of Natural Sciences

|

2015

|

tom 03

EN

The present investigation was carried out to evaluate the hypolipidemic activity of methanolic and aqueous leaf extracts of Cassia fistula (CF) Linn (Family: Fabaceae) on atherogenic diet (2% cholesterol, 1% Choline Chloride and 2% Lard) induced Male Sprauge-Dawley rats for the period of 21 days. The rats receiving the treatment with leaf methanol and aqueous extract of CF showed significant (P < 0.001) reduction in total cholesterol (TC), triglycerides (TG), High density lipoprotein (HDL), lipid peroxidation (LPO) and other parameters at dose dependent manner but the effect was less than the standard drug Atorvastatin. The results revealed the effectiveness of CF plant against hyperlipidemic activity.

9

Antimicrobial and antioxidant activity of the essential oil and methanol extract of Nepeta cataria

72%

Adiguzel A. , Ozer H. , Sokmen M. , Gulluce M. , Sokmen A. , Kilic H. , Sahin F. , Baris O.

Polish Journal of Microbiology

|

2009

|

tom 58

|

nr 1

69-76

EN

Catnip (Nepeta cataria) is an important medicinal herb belonging to the mint family, Lamiaceae. In this study, the in vitro antimicrobial and antioxidant activities of the essential oil and methanol extract from Nepeta cataria, and its essential oil composition were investigated. The essential oil, which has 4aα,7α,7aβ-nepetalactone (70.4%), 4aα,7α,7aβ-nepetalactone (6.0%), thymol (2.3%), and 4aα,7α, 7aβ-nepetalactone (2.5%), as main components, exhibited activity against eleven bacteria, and twelve fungi and a yeast, C. albicans; with Minimum Inhibitory Concentrations (MIC) values ranging from 12.50 to 250 μl/ml; the methanol extract showed weaker activity. The samples were also subjected to a screening for their possible antioxidant activities by using 2.2-diphenyl-1-picrylhydrazyl (DPPH) and β-carotene/linoleic acid assays. In DPPH assay, the extract showed slight antioxidant activity whereas the essential oil remained inactive. In the latter case, both the extract and the essential oil exerted weak activity having inhibiton ratios of linoleic acid oxidation at 16.4% and 27.0%, respectively. The weak antioxidative nature of the extract could be attributed to the low phenolic content, estimated as gallic acid equivalent at 22.6 ± 2.07 μg/ml or 2.26%. In both systems, antioxidant capacity of BHT was determined in parallel experiments.

10

Evaluation of antidote potential of methanol leaf extract of Bauhinia monandra on heparin-induced thrombocytopenia in mice

72%

Nworie K. M. , Okorie A. N. , Ebere D. N. , Obike O. C.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

|

2019

|

tom 100

|

nr 1

11

Effect methanolic extract of Achyranthus aspera on biochemical status of Albino mice

72%

Reddy C. V. , Kamble A.

International Letters of Natural Sciences

|

2014

|

tom 09

EN

The effect of administration of Methanolic extract of Achyranthsu asper on biochemical parameter of mice were investigated. This drug induced significant reduction in the cholesterol level and amount of RNA. But significant increase in the amount of glycogen at 25 mg/kg and no significant reduction in the amount of glycogen at 50 mg/kg. This drug also induced increase in the level of alkaline phospatese and decrease in the level of acid phosphatase, protein and DNA, but these changes are statistically not significant. Protein 25 mg shows 4.22 ±0.112 and 50 mg shows 4.05 ±0.085. Cholesterol 25mg shows 0.5 ±0.04 and 50 mg shows 0.39 ±0.038. Glycogen 25mg shows 0.288 ±0.011 and 50mg shows 0.248 ±0.177. DNA 25mg shows 0.109 ±0.039 and 50 mg shows 0.085 ±0.035, RNA 25mg shows 0.38 ±0.036 and 50 mg shows 0.25 ±0.031

12

Toxicity study of Achyranthus aspera

72%

Reddy C. V. , Kamble A.

International Letters of Natural Sciences

|

2014

|

tom 09

EN

The objectives of present work focuses to check the acute and sub acute toxicity studies for the Achyranthes aspera L methanol extract as per OECD guideline in Swiss mice. The dose of 100, 200, 250 gm/kg body weight methanol extract by administering drug intraperitoneally and recorded the growth, body weight, organ weight, general symptoms, morphological and physiological behavior, mortality. These parameters on various organs system in mice were studied. It was considered necessary to assess its potential health hazard in man and to find the safe and effective dose. The observations of changes in body weight, food and water intake as well as cage side observations were reported. There was no abnormality observed in all groups. The whole plant powder methanol extract of Achyranthes Aspera L were found to be nontoxic.

13

Syringin in some species of the subtribe Centaureinae of the Asteraceae

72%

Cis J. , Nowak G. , Horoszkiewicz-Hassan M. , Kisiel W.

Acta Societatis Botanicorum Poloniae

|

2003

|

tom 72

|

nr 2

EN

The occurrence of syringin in eight out of ten investigated plant species of the subtribe Centaureinae (Asteraceae) was reported. The compound was isolated from aerial parts of the plants by silica gel column chromatography of methanol extracts. Contents of syringin varied from 0.001 to 0.1% of the dried plant material. Centaurea bella Trautv. appeared to be the best source of this compound (0.1% dry wt.). Syringin has been reported for the first time from these plant species.

14

Chemical analysis mycelial culture of Calocera viscosa [Pers.:Fr.] Fr.

72%

Wegiel J. , Szewczyk A. , Sulkowska-Ziaja K.

Acta Physiologiae Plantarum

|

1997

|

tom 19

|

nr 2

15

Evaluation of antioxidant activity of in vitro propagated medicinal Ceratophyllum demersum L. extracts

58%

Emsen B. , Dogan M.

Acta Scientiarum Polonorum. Hortorum Cultus

|

2018

|

tom 17

|

nr 1

EN

Ceratophyllum demersum L. is a medical plant of the family Ceratophyllaceae that has been traditionally used for the treatment of diseases such as ulcer, diarrhoea, wounds and fever since ancient times. This study presents antioxidant activities of water and methanol extracts of in vitro propagated C. demersum. Shoot tip explants of C. demersum for in vitro plant regeneration were cultured on liquid Murashige and Skoog (MS) medium containing 0.25–1.25 mg/L 6-Benzylaminopurine (BAP) and 0.10 mg/L Thidiazuron (TDZ) combinations for eight weeks. Maximum number of plantlets per explant (110.67) was obtained on MS medium supplemented with 0.75 mg/L BAP + 0.10 mg/L TDZ. In order to determine antioxidant activities of C. demersum, antioxidant contents (β-carotene, flavonoid, lycopene and total phenols), metal chelating and reducing power capacities of methanol and water extracts obtained from C. demersum were investigated. It was detected that water extract was more effective in all activities. Total phenols (76.55 μg/mg) in water extract were measured as the highest antioxidant component. Based on the water extracts data, as IC50 value for chelating on ferrous ions was 9.24 mg/mL, EC50 value for reducing power activity was 8.23 mg/mL. It was also determined that the metal chelating and reducing power activities of the both extracts are increased depending on the concentration. Therefore, all these data suggest that both tested extracts of in vitro propagated C. demersum would exert beneficial effects by virtue of their antioxidant properties and may be utilized as a promising source of therapeutics.

16

Gas chromatographic-mass spectrometric investigation of the chemical composition of the aquatic plant Wolffia arrhiza (Lemnaceae)

58%

Kotowska U. , Piotrowska A. , Isidorova A. G. , Bajguz A. , Isidorov V. A.

Oceanological and Hydrobiological Studies

|

2013

|

tom 42

|

nr 2

17

Phytochemical and antibacterial evaluation of various extracts of Amoora ruhituka bark

58%

Umesh M. K. , Sanjeevkumar C. B. , Londonkar R.

International Letters of Natural Sciences

|

2015

|

tom 39

EN

Preliminary phytochemical screening of various extracts of Amoora ruhituka bark were investigated which reveals the presence of several secondary metabolites in each extracts. The antibacterial activity of all the extracts was tested against four gram negative bacterial strains. The results indicated the zone of inhibition which ranges from 11.30±577 to 18.7±0.577 for different extracts in which Methanol extract has shown highest zone of inhibition for Salmonella typhimurium followed by Enterobacter aerogenes , E coli and P aeruginosa whereas benzene extract has showed the least zone of inhibition and the minimum inhibitory concentration (MIC) of the different extracts ranging from 0.78 mg/ml to 6.25mg/ml.The complete results of this study provides a essential data for the use of Amoora ruhituka for the treatment of infection associated diseases.

18

Starch-iodine assay method underestimates alpha-amylase inhibitory potential of antioxidative compounds and extracts

58%

Ononamadu C. J. , Ezeigwe O. C. , Owolarafe T. A. , Ihegboro G. O. , Lawal T. A. , Salawu K. , Umeoguaju U. F. , Aminu I.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

|

2020

|

tom 101

|

nr 1

19

Antimicrobial activity of chlorophyll and methanol extract of Lannea coromandelica Merr.

44%

Vedhanarayanan P. , Vaithiyanathan T. , Sundaramoorthy P.

International Letters of Natural Sciences

|

2015

|

tom 45

EN

The antimicrobial activity of chloroform and methanol extracts of Lennea coromandelica were screened for their was studied against gram positive bacteria strains Staphylococcus aureus, Streptococcus pyogenes and Bacillus subtilis gram negative bacteria strains Pseudomonas aeruginosa, Klebsiella pneumoniae and Escherichia coli and fungal strains such as Candida albicans, C. parapsilosis, C. krusei and C. glabrata using disc diffusion method, determination of Minimum Inhibitory Concentrations (MIC), Minimum Bacterial Concentrations (MBC) and Minimum Fungicidal Concentrations (MFC). The antimicrobial activity for different concentrations like 250 μg, 500 μg and 1000 μg of different solvent extracts of Lannea cormandelica. bacterial strains and recorded in highest mean zones of inhibition ranged from 19.6 mm and Candidal strains and the exhibited the highest mean zones of inhibition ranged from 10.6 mm. Methanol extracts showed the best results as inhibition zones against tested organisms. Results showed also that, the greatest effect was towards Staphylococcus aureus and the lowest was against Candida krusei. The present study reported the great effect of Lannea cormandelica extracts against some of most important pathogens.