Wyniki wyszukiwania - Biblioteka Nauki

1

Do arbuscular mycorrhizal fungi affect metallothionein MT2 expression in Brassica napus L. roots?

100%

Dabrowska G. , Hrynkiewicz K. , Trejgell A.

Acta Biologica Cracoviensia. Series Botanica

|

2012

|

tom 54

|

nr 1

EN

Arbuscular mycorrhizal fungi are the most widespread root fungal symbionts, forming associations with the vast majority of plant species. Ectomycorrhizal development alters gene expression in plant symbionts. In this work we examined the effect of arbuscular mycorrhizal fungi spores on the growth and development of Brassica and on the expression of BnMT2 in winter rape. In a pot experiment, rape seedlings growing on different types of sterile and nonsterile soils were inoculated simultaneously with mycorrhizal fungi spores of Acaulospora longula, Glomus geosporum, Glomus mosseae and Scutellospora calospora. As compared with control plants growing in the absence of spores, ten-week-old seedlings of Brassica napus L. in sterile soil inoculated with arbuscular spores had longer shoots and higher fresh biomass of above-ground plant parts. In other types of substrates enriched with mycorrhizal fungi spores, the plants were smaller than non-inoculated plants. The presence of AMF spores stimulated the elongation growth of hypocotyls in both analyzed substrates. BnMT2 expression was highest in plants growing on the sterile substrate. Generally, the presence of mycorrhizal fungi spores appeared to have an adverse effect on the growth of rape plants.

2

Do Arbuscular Mycorrhizal Fungi Affect Metallothionein Mt2 Expression In Brassica Napus L. Roots?

100%

Dąbrowska G. , Hrynkiewicz K. , Trejgell A.

|

3

Stress proteins induced by cadmium in the abdominal muscle of the shrimp Crangon crangon [commun.]

100%

Napierska D. , Radłowska M.

Oceanologia

|

1998

|

tom No. 40 (2)

157-162

EN

Shrimps were exposed to various concentrations of CdCl2 under laboratory conditions for 96 h. Abdominal muscles were isolated from exposed and control animals. The induction of stress proteins (heat shock proteins of the Hsp70 family and metallothionein) was detected following polyacrylamide gel electrophoresis in the presence of SDS (SDS-PAGE) and specific staining (Western blotting method in the case of the Hsp70 or Coomassie blue and silver staining in the case of metallothionein). The short-term cadmium poisoning in the shrimp Crangon crangon resulted in the induction, in a concentration-dependent manner, of metallothionein and a new protein with an approximate molecular weight of 70 kDa in abdominal muscle. This protein was immunologically cross-reactive with the 70 kDa heat shock protein of the mouse.

4

Serum metallothionein in newly diagnosed patients with childhood solid tumours

100%

Krizkova S. , Masarik M. , Majzlik P. , Kukacka J. , Kruseova J. , Adam V. , Prusa R. , Eckschlager T. , Stiborova M. , Kizek R.

Acta Biochimica Polonica

|

2010

|

tom 57

|

nr 4

561-566

EN

Tumour markers are substances produced by malignant cells or by the organism as a response to cancer development. Determination of their levels can, therefore, be used to monitor the risk, presence and prognosis of a cancer disease or to monitor the therapeutic response or early detection of residual disease. Time-consuming imaging methods, examination of cerebrospinal fluid or tumour tissue and assays for hormones and tumour markers have been used for cancer diagnosis. However, no specific marker for diagnosis of childhood solid tumours has been discovered yet. In this study, metallothionein (MT) was evaluated as a prospective marker for such diseases. Serum metallothionein levels of patients with childhood solid tumours were determined using differential pulse voltammetry - Brdicka reaction. A more than 5-fold increase in the amount of metallothionein was found in sera of patients suffering from cancer disease, compared with those in sera of healthy donors. The average metallothionein level in the sera of healthy volunteers was 0.5 ± 0.2 μmol · dm-3 and was significantly different (p<0.05, determined using the Schefe test) from the average MT level found in serum samples of patients suffering from childhood solid tumours (3.4 ± 0.8 μmol · dm-3). Results found in this work indicate that the MT level in blood serum can be considered as a promising marker for diagnostics, prognosis and estimation of therapy efficiency of childhood tumours.

5

Expression of metallothionein [MT] and glutathione s-transferase pi [SGTP] in the bone marrow of patients with myeloproliferative disorders [MPD]

100%

Wrobel T. , Mazur G. , Dziegiel P. , Surowiak P. , Kuliczkowski K. , Zabel M.

Folia Morphologica

|

2004

|

tom 63

|

nr 1

EN

Overexpression of SGTP and/or MT may contribute to various carcinogenic processes and to resistance to anticancer treatment. The importance of these proteins, although clearly established in solid tumours, has not been fully understood in haematopoietic neoplasm. The aim of this study was to determine the expression of MT and SGTP in the bone marrow of patients with MPD. Twenty paraffin-embedded bone marrow core biopsy specimens from newly diagnosed patients with MPD were evaluated — osteomyelofibrosis (OMF), n = 9 and chronic myelocytic leukaemia (CML), n = 11. We demonstrate increased SGTP and MT expression in the bone marrow of MPD patients. In our study levels of MT in OMF patients were higher than in CML. This suggests that MT expression may correlate with bone marrow fibrosis. These data, although based on a relatively small number of patients, raise the possibility that SGTP and MT may play a role in the pathogenesis of MPD. The clinical significance of this phenomenon needs further investigation.

6

Plant metallothineins: putative functions identified by promoter analysis in silico

86%

Dabrowska G. , Mierek-Adamska A. , Goc A.

Acta Biologica Cracoviensia. Series Botanica

|

2012

|

tom 54

|

nr 2

EN

Metallothioneins are low-molecular-weight proteins capable of covalently binding heavy metal ions due to the presence of many cysteine residues in their sequences. We analyzed the predicted amino acid sequences of 19 metallothionein (7 from Arabidopsis thaliana and 12 from Oryza sativa) and their promoter sequences in silico in order to determine the potential regulatory cis-elements present in the promoters of metallothionein genes, from which it is possible to determine the putative functions of these genes. The PlantCARE and PLACE databases provided information about the putative regulatory elements in the metallothionein promoters. Metal response element sequences were found in the promoters of eleven O. sativa and two Arabidopsis metallothionein genes. Copper response elements were identified in both model plants, usually in many copies, particularly in O. sativa. Both the high cysteine content and the presence of metal response motifs in the promoters support the suggestion that metallothioneins play a key role in metal detoxification. The most common putative element in the analyzed promoters was CIRCADIAN, which was present in five A. thaliana and eight O. sativa sequences. The methyl jasmonate response sequence, root-specific expression element and drought response element were found only in O. sativa metallothioneins. Light and low temperature response elements, biotic and abiotic stress elements, an abscisic acid-responsive element and an ethylene-responsive element occur in selected metallothionein promoters of both species. A few promoters have putative organ- and cell-specific regulatory elements. The presence of many different motifs in the promoters of the Arabidopsis and O. sativa genes implies that metallothioneins are general stress response proteins with many important functions in plants, including regulation of their normal development and adaptation to changing environmental conditions.

7

Role of zinc in nervous system cells

86%

Florianczyk B.

Journal of Pre-Clinical and Clinical Research

|

2011

|

tom 05

|

nr 1

8

The expression of metallothionein compared to the expression of Ki-67 proliferative antigens in cells of stratified non-keratinising epithelium and in cells of planoepithelial carcinoma of the uterine cervix

86%

Surowiak P. , Dziegiel P. , Zabel M. , Wojnar A.

Cellular and Molecular Biology Letters

|

2002

|

tom 07

|

nr Suppl.

9

The application of covalent affinity chromatography with thiol-disulphide interchange for quantitative determination of metallothionein-specific protein from human fluids, as indicator of environmental exposure to heavy metals

86%

Kabzinski A. K. M.

Polish Journal of Environmental Studies

|

1997

|

tom 06

|

nr 5

EN

The aim of this study was to present a new analytical method for the quantitative determination of metallothioneins (MT) protein in human body fluids and tissues, in order to determine the level of environmental and industrial exposition to heavy metals. For MTs isolation covalent affinity chromatography with thiol-di- sulphide interchange (CAC-TDI) was applied, which is a modern technique of separation of a high affinity, good repeatability and reproducibility, allowing specific isolation of the thiolproteins and metallothiolproteins. Fundamentals of indirect determination of the contents of metallothioneins protein were worked out throught estimation of the quantities of metals bound with metallothionein protein and adsorbed on covalent affinity chromatography gel as on solid-phase extraction support during a separating process. The (CAC-TDI) gel, specially prepared, was used as a solid phase extraction support (SPE) for preconcent- ration of Hg-Thionein (Hg-Th), Cd-thionein (Cd-Th), Zn-thionein (Zn-Th) and Cu-thionein (Cu-Th) proteins and Hg, Cd, Zn and Cu bonded with MTs from spiked water, urine, human plasma, breast milk and tissues' homogenates.

10

Expression of metallothionein in renal tubules of rats exposed to acute and endurance exercise

86%

Podhorska-Okolow M. , Dziegiel P. , Dolinska-Krajewska B. , Dumanska M. , Cegielski M. , Jethon Z. , Rossini K. , Carraro U. , Zabel M.

Folia Histochemica et Cytobiologica

|

2006

|

tom 44

|

nr 3

11

The influence of ethanol on zinc concentration in Cd-exposed rats

86%

Galazyn-Sidorczuk M. , Brzoska M. M. , Moniuszko-Jakoniuk J. , Kowalczyk M.

Polish Journal of Environmental Studies

|

1998

|

tom 07

|

nr 1

EN

The aim of this study was to examine the influence of oral intoxication with cadmium and ethanol separately or together on zinc concentrations in liver and kidney of rats. Cadmium was administered at a concentration of 50 mg Cd/dm3 in drinking water for 8 weeks. Ethanol was given in a dose of 5 g/kg of body weight (as a 25% solution) every 12 h for the last 108 h of the experiment. The conducted study has shown that intoxication with cadmium and ethanol alone as well as in combination leads to an increase in zinc concentrations in the liver and kidney. The elevation of zinc levels is highest when the rats are co-exposed to cadmium and ethanol. The deposition of zinc in the liver and kidney of cadmium intoxicated rats is associated with cadmium accumulation and induction of metallothionein synthesis in these organs. Ethanol is also able to induce metallothionein synthesis and an elevation of zinc concentrations in the liver and kidney. As a result, in conditions of co-exposure these effects sum up.

12

Metallothioneins in rats exposed to barium chloride

86%

Florianczyk B. , Staroslawska E.

Bulletin of the Veterinary Institute in Puławy

|

2003

|

tom 47

|

nr 1

13

Zastosowanie zmodyfikowanych bakterii glebowych do poprawy skuteczności fitoremediacji środowiska zanieczyszczonego jonami metali śladowych

72%

Dąbrowska G. , Hrynkiewicz K. , Janczak K. , Żurańska M.

Ochrona Środowiska

|

2014

|

tom Vol. 36, nr 1

21--26

EN

Eight bacterial strains from areas contaminated with trace metals were selected for the experiments: Bacillus sp., Bacteroidetes bacterium, Flavobacterium sp., Luteibactor rhizovicina, Pseudomonas fluorescens, Pseudomonas sp., Serratia entomophila and Variovorax sp. Bacteria were transformed with pET21a expression plasmid encoding Brassica napus L. metallothionein 2 (BnMT2). Transformant colonies were examined for growth on solid culture medium containing trace metal ions (Cd and Pb). It was found that most of the analyzed bacterial strains were resistant to ampicillin, though the antibiotic inhibited their growth. It was demonstrated that growth of bacterial strains on a medium containing Pb ions was not inhibited, while Cd ion content limited or inhibited growth of some bacterial strains. No significant differences were noted in growth between transformed and untransformed bacteria of a given strain on the medium containing Pb ions. The BnMT2 transgene presence abolished adverse effects of Cd ions on Flavobacterium sp., Variovorax sp. and L.rhizovicina. Modified bacterial strains were demonstrated for potential use in treatment of soil contaminated with trace metals, especially Cd ions.

PL

W przeprowadzonych badaniach wykorzystano osiem szczepów bakterii: Bacillus sp., Bacteroidetes bacterium, Flavobacterium sp., Luteibactor rhizovicina, Pseudomonas fluorescens, Pseudomonas sp., Serratia entomophila, Variovorax sp. wyizolowanych z terenów zanieczyszczonych jonami metali śladowych. Bakterie stransformowano plazmidem ekspresyjnym pET21a zawierającym fragment genu kodujący metalotioneinę typu 2 Brassica napus L. (BnMT2). Po uzyskaniu kolonii bakteryjnych (transformantów) sprawdzono ich wzrost na podłożu stałym zawierających jony metali śladowych (Cd i Pb). Stwierdzono, że większość analizowanych szczepów bakterii wykazuje oporność na ampicylinę, chociaż antybiotyk ogranicza ich wzrost. Wykazano, iż wzrost badanych szczepów na podłożu zawierającym jony ołowiu nie był hamowany, natomiast jony kadmu ograniczały lub hamowały wzrost niektórych szczepów bakterii. Nie stwierdzono znacznych różnic we wzroście pomiędzy bakteriami transformowanymi i nietransformowanymi danego szczepu na podłożu zawierającym jony ołowiu. Obecność transgenu BnMT2 niwelowała szkodliwe działanie jonów kadmu w przypadku Flavobacterium sp., Variovorax sp. i L. rhizovicina. Wykazano, że zmodyfikowane szczepy bakterii mogą znaleźć zastosowanie w procesie oczyszczania gleby zawierającej jony metali śladowych, a zwłaszcza kadmu.

14

Expression of metallothionein I/II and Ki-67 antigen in various histological types of basal cell carcinoma

72%

Bieniek A. , Pula B. , Piotrowska A. , Podhorska-Okolow M. , Salwa A. , Koziol M. , Dziegiel P.

Folia Histochemica et Cytobiologica

|

2012

|

tom 50

|

nr 3

15

Determination of metallothionein in biological fluids using enzyme-linked immunoassay with commercial antibody

72%

Milnerowicz H. , Bizon A.

|

tom 57

|

nr 1

99-104

EN

Metallothionein (MT) is a low molecular weight cysteine-rich protein with a number of roles in the pro/antioxidant balance and homeostasis of essential metals, such as zinc and copper, and in the detoxification of heavy metals, such as cadmium and mercury. Until now, detection of metallothionein in biological fluids remained difficult because of a lack of a broadly reactive commercial test. Meaningful comparison of the values of metallothionein concentrations reported by different authors using their specific isolation procedures and different conditions of enzyme-linked immunoassay is difficult due to the absence of a reference material for metallothionein. Therefore in the present study, we describe a quantitative assay for metallothionein in biological fluids such as plasma and urine performed by a direct enzyme-linked immunoassay using a commercially; available monoclonal mouse anti-metallothionein clone E9 antibody and commercial standards of metallothionein from rabbit liver and a custom preparation of metallothionein from human liver. The sensitivity of the assay for the standard containing two isoforms MT-I and MT-II from human liver was 140 pg/well. The reactivity of the commercial standards and standards containing two isoforms MT-I and MT-II isolated from human liver in our laboratory with a commercial monoclonal mouse anti-metallothionein clone E9 antibody were similar. This suggests that the described ELISA test can be useful for determination of metallothionein concentration in biological fluids. The concentrations of metallothionein in human plasma, erythrocyte lysate and in urine of smoking and non-smoking healthy volunteers are reported. Tobacco smoking increases the extracellular metallothionein concentration (plasma and urine) but does not affect the intracellular concentration (erythrocyte lysate).

16

Analiza porównawcza immunoreaktywnosci metalotioneiny w zasniadzie groniastym i w doczesnej w zaleznosci od zastosowanego leczenia – wyłacznie operacyjnego albo operacyjnego z uzupełniajaca chemioterapia

72%

Basta P. , Dutsch-Wicherek M. , Biedka M. , Koper K. , Mach P.

Current Gynecologic Oncology

|

2012

|

tom 10

|

nr 4

261-269

EN

Introduction: The presence of metallothionein in both the endometrium and decidua is associated with the resis- tance of both endometrial and decidual cells to immune-mediated apoptosis. Consequently, the aim of the pres- ent study has been to typify metallothionein immunoreactivity within the molar lesions of both the patients who were treated with surgery alone and those who were also given chemotherapy. Methods: We analyzed the immu- noreactivity of metallothionein in both the trophoblast and decidual cells derived from patients on whom curet- tage was performed due to a diagnosis of complete molar pregnancy. These patients were then divided into two subgroups according to whether or not they required chemotherapy following the surgical procedure. Results: We observed a statistically significantly lower metallothionein immunoreactivity levels within the trophoblast cells derived from the complete hydatidiform mole patients who had had surgery alone in comparison to the levels found in those patients for whom surgery was followed by chemotherapy. Conclusion: Resistance to apoptosis linked with metallothionein intracellular immunoreactivity may influence the clinical course of hydatidiform mole.

PL

Wprowadzenie: Obecność metalotioneiny w endometrium i doczesnej, czynnika antyapoptotycznego, jest zwią- zana z opornością na apoptozę zarówno komórek endometrium, jak i komórek doczesnej zależną od aktywności komórek układu odpornościowego. Celem niniejszego badania była ocena immunoreaktywności metalotioneiny w ogniskach zaśniadu u pacjentek leczonych wyłącznie operacyjnie i u chorych, u których dodatkowo zastosowa- no chemioterapię. Metoda: Analizowano immunoreaktywność metalotioneiny zarówno w komórkach trofoblastu, jak i w komórkach błony doczesnej uzyskanych w wyniku łyżeczkowania jamy macicy przeprowadzonego z powo- du rozpoznania ciąży zaśniadowej całkowitej. Pacjentki podzielono na dwie podgrupy: chorych, które wymaga- ły dodatkowej chemioterapii po zabiegu operacyjnym, oraz chorych, u których takiego leczenia nie zastosowano. Wyniki: Stwierdzono statystycznie znamienny niższy poziom immunoreaktywności metalotioneiny w obrębie ko- mórek trofoblastu pochodzących od pacjentek z zaśniadem groniastym całkowitym leczonych wyłącznie operacyj- nie w porównaniu z pacjentkami, u których leczenie operacyjne uzupełniono chemioterapią. Wniosek: Oporność na apoptozę może zależeć od wewnątrzkomórkowej obecności metalotioneiny, co prawdopodobnie ma wpływ na przebieg kliniczny zaśniadu groniastego.

17

Location of heat shock protein 70 and metallothionein immunoreactivity associated with copper deficiency in the CNS of lambs

72%

Tunca R. , Erginsoy S. D. , Sozmen M. , Guvenc T. , Ercag E.

Medycyna Weterynaryjna

|

2007

|

tom 63

|

nr 10

1187-1192

EN

The localizations of metallothionein I and II (MT), a small molecular weight heavy metal binding proteins, and 70-kDa heat shock protein (Hsp70) were investigated by immunohistochemical techniques in brains of lambs that had been injured by congenital copper deficiency. The results were compared with those obtained from control lambs. The morphological findings of the congenital copper deficiency in the central nervous system (CNS) were recorded. The amount of copper in the brain and liver of the lambs and feed of breeding ewes and soil was also assayed by atomic absorption spectrophotometry. The amount of copper in the brain, liver, soil and feed were low. Immunohistochemically, MT and Hsp70 expressions were found to be markedly increased in the CNS of congenital copper deficient lambs compared with control lambs. MT immunoreactivity was prominently found in the astrocytes while strong Hsp70 labelling was in both astrocytes and neurons in the cerebrum, cerebellum, thalamus/hypothalamus and medulla oblongata. Immunohistochemical labelling for both MT and Hsp70 was also seen in the pia mater, ependymal cells and choroid plexi. Present results suggest that the elevated expressions of MT and Hsp70 in astrocytes and neurons are possibly indicating that they are less susceptible to the consequences of cell stress factors and could be exploited to increase selectively their survival in copper deficiency.

18

Evaluation of apoptosis, proliferation intensity and metallothionein [MT] expression in comparison with selected clinicopathological variables in primary adenocarcinomas of the large intestine

72%

Dumanska M. , Dziegiel P. , Sopel M. , Wojnar A. , Zabel M.

Folia Morphologica

|

2004

|

tom 63

|

nr 1

EN

Tumour growth and expansion are the result of proliferative activity and the capacity to eliminate cells by apoptosis and/or necrosis. The present study was aimed at comparing the apoptosis and proliferation intensity in cells of adenocarcinomas of the large intestine with the expression of metallothionein (MT), the grade of the tumour and the depth to which the tumour infiltrated the intestinal wall. The TUNEL technique and immunocytochemical reactions (expression of caspase-3, Ki-67, MT) were used to detect apoptosis. The results demonstrated augmented levels of all the variables examined, positively correlated with grade of malignancy, G, and with the depth of intestinal wall infiltration by the tumour cells. The testing of apoptosis, proliferation and MT expression may prove useful in the appraisal of the growth and progression of primary adenocarcinomas in the large intestine.

19

Investigation of metallothionein concentration with preconcentration by solid phase extraction on covalent affinity chromatography gel for quantitative determination of exposure to heavy metals

72%

Kabzinski A. K. M.

Polish Journal of Environmental Studies

|

1996

|

tom 05

|

nr 5

EN

The aim of this study was to test a new method for the quantitative determination of metallo- thionein (MT), in order to determine the level of exposure to heavy metals. For MT preconcentration, the solid phase extraction (SPE) method with covalent affinity chromatography with thiol-disulphide interchange (CAC-TDI) gel was applied. In experiment Cd-thionein (Cd-Th) obtained from rats exposed to cadmium salt was used. Protein concentration was determined by indirect method based on metal analysis, bonded with MT protein by atomic absorption spectrometry (AAS). The results showed satisfactory correlation between Cd-Th concentration and the level of exposition for heavy metals.

20

Tissue specific expression of beta-glucuronidase gene driven by heterologous promoters in transgenic cauliflower plants

72%

Baranski R. , Puddephat I. J.

Acta Physiologiae Plantarum

|

2004

|

tom 26

|

nr 3