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The proteome analysis of rat platelet with nano-liquid chromatography-matrix-assisted laser desorption/ionization time-of-flight mass spectrometry technique

100%

Gromotowicz-Poplawska A. , Kasprzyk J. , Marcinczyk N. , Stepien E. , Piekoszewski W. , Chabielska E.

Journal of Physiology and Pharmacology

2018

tom 69

nr 6

Desorption-ionization on silicon for small molecules: a promising alternative to MALDI TOF

84%

Kraj A. , Dylag T. , Gorecka-Drzazga A. , Bargiel S. , Dziuban J. , Silberring J.

Acta Biochimica Polonica

2003

tom 50

nr 3

A method has been developed for laser desorption/ionization of catecholamines from porous silicon. This methodology is particularly attractive for analysis of small molecules. MALDI TOF mass spectrometry, although a very sensitive technique, utilizes matrices that need to be mixed with the sample prior to their analysis. Each matrix produces its own background, particularly in the low-molecular mass region. Therefore, detection and identification of molecules below 400 Da can be difficult. Desorption/ionization of samples deposited on porous silicon does not require addition of a matrix, thus, spectra in the low-molecular mass region can be clearly readable. Here, we describe a method for the analysis of catecholamines. While MALDI TOF is superior for proteomics/peptidomics, desorption/ionization from porous silicon can extend the operating range of a mass spectrometer for studies on metabo- lomics (small organic molecules and their metabolites, such as chemical neurotransmitters, prostaglandins, steroids, etc.).

Oligosaccharide structures of N-cadherin from human normal and cancer bladder cell lines by matrix-assisted laser desorption mass spectrometry

67%

Hoja-Lukowicz D. , Litynska A. , Amoresano A. , Ciolczyk-Wierzbicka D. , Laidler P.

Acta Biologica Cracoviensia. Series Zoologia

2007

tom 49

31-39

Defining the blood plasma protein repertoire of seven day old dairy calves - a preliminary study

67%

Skrzypczak W. F. , Ozgo M. , Lepczynski A. , Herosimczyk A.

tom 62

nr 3

During the early postnatal period in calves various adaptational changes occur. These functional, morphological and also metabolic alteration are reflected by blood plasma protein changes as they are secreted and shed from many cells and tissues. Blood plasma protein pattern of an adult cattle differs in some respect when compared with neonatal calves. There exist a very few data concerning 2-D maps of neonatal calves blood plasma. The above prompted us to establish protein pattern of this biological fluid characteristic of healthy, 7 day old, Polish Black-and-White (Polish Friesian) breed calves. Blood plasma proteins of the isoelectric point ranging from 4.0 to 7.0 were analyzed by the aid of high resolution two-dimensional electrophoresis (2-DE). Subsequently, 79 excised protein spots corresponding to 23 different gene products were identified using matrix-assisted laser desorption/ionisation mass spectrometer (MALDI-TOF MS). Protein map obtained in the present study may be useful in assessing the changes in the calves blood plasma protein profiles occurring in response to different physiological and/or pathophysiological factors.