Wyniki wyszukiwania - Biblioteka Nauki

1

Spatial differences of matrix metalloproteinase-2 and matrix metalloproteinase-9 within abdominal aortic aneurysm wall and intraluminal thrombus

100%

Siennicka A. , Zuchowski M. , Kaczmarczyk M. , Cnotliwy M. , Clark J. S. , Jastrzebska M.

Journal of Physiology and Pharmacology

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2016

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tom 67

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nr 6

2

In vitro reoxygenation following hypoxia increases MMP-2 and TIMP-2 secretion by human umbilical vein endothelial cells

100%

Cavdar Z. , Oktay G. , Egrilmez M. Y. , Genc S. , Genc K. , Altun Z. , Islekel H. , Guner G.

Acta Biochimica Polonica

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2010

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tom 57

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nr 1

69-73

EN

Endothelial cells lining the inner blood vessel walls play a key role in the response to hypoxia, which is frequently encountered in clinical conditions such as myocardial infarction, renal ischemia and cerebral ischemia. In the present study we investigated the effects of hypoxia and hypoxia/reoxygenation on gelatinases (matrix metalloproteinase-2 and -9), their inhibitor (TIMP-2) and activator (MT1-MMP), in human umbilical vein endothelial (HUVE) cells. HUVE cells were subjected to 4 h of hypoxia or hypoxia followed by 4 and 24 h of reoxygenation. The pro- and active forms of MMP-2 and MMP-9 were analyzed by gelatin zymography; TIMP-2 protein level was assayed using ELISA, while MT1-MMP activity was measured using an activity assay. The secretion of MMP-2 proform increased significantly in cells subjected to 4h of hypoxia followed by 4 or 24 h of reoxygenation, compared with the normoxic group. TIMP-2 protein level also increased significantly in the hypoxia/reoxygenation groups, compared with the normoxic group. There were no statistically significant differences in the levels of active MT1-MMP in all groups. This study indicates that MMP-2 and TIMP-2 could be regarded as important components of a mechanism in the pathophysiology of ischemic injury following reperfusion.

3

Uwarunkowania genetyczne rozszczepu wargi górnej i/lub podniebienia - czy polimorfizm genu MMP2 ma znaczenie w rozwoju tej wady?

100%

Zalewska-Ziob M. , Adamek B. , Kasperczyk J. , Łyko D. , Płachetka A. , Rokicki M. , Machorowska-Pieniążek A. , Baron S. , Niedzielska I. , Wiczkowski A.

Pediatria i Medycyna Rodzinna

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2014

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tom 10

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nr 3

306-314

EN

Introduction: Cleft lip/palate is one of the most common congenital malformations. In Poland, approximately 500 children with an orofacial cleft are born every year. Matrix metalloproteinases are involved in periodontal tissue remodelling and degradation. Polymorphisms in the promoter region of the MMP2 gene may affect transcription and activity of the protein produced by this gene. The aim of the study was to examine 1306 C/T MMP2 gene promoter polymorphisms in the group of children with cleft lip/palate and in the control group as well as to determine the frequency of individual genotypes in different types of orofacial clefts. Material and methods: The study was conducted in the group of 150 children with cleft lip/palate and 102 children without an orofacial cleft. Genomic DNA was obtained from oral mucosa epithelium. The MMP2 gene promoter polymorphism was genotyped by tetra-primer ARMS-PCR. Results: There are no significant differences in the frequency of individual alleles in different types of orofacial clefts. The occurrence of the CC genotype was significantly higher in the group with cleft lip and palate than in the healthy group (p = 0.005). Conclusion: Determining the polymorphism of matrix metalloproteinase gene promoter sequence can contribute to the elucidation of cleft lip/palate aetiopathogenesis.

PL

Wstęp: Rozszczep wargi górnej i/lub podniebienia jest jedną z najczęściej występujących wad rozwojowych. W Polsce każdego roku przychodzi na świat około 500 dzieci z rozszczepem w okolicy twarzoczaszki. W proces tworzenia struktur twarzoczaszki zaangażowane są metaloproteinazy macierzy zewnątrzkomórkowej, odpowiedzialne za przebudowę i degradację podłoża łącznotkankowego. Polimorfizm w regionie promotorowym genu kodującego MMP2 może mieć wpływ na efektywność transkrypcji, a tym samym na aktywność produktu białkowego tego genu. Celem niniejszej pracy była ocena polimorfizmu 1306 C/T promotora genu MMP2 w grupie dzieci z rozszczepem wargi i/lub podniebienia oraz w grupie kontrolnej, a także ocena częstości występowania poszczególnych genotypów w różnych typach rozszczepów. Materiał i metoda: Badanie przeprowadzono w grupie 150 dzieci z rozpoznanym rozszczepem wargi i/lub podniebienia oraz w grupie 102 dzieci bez wady rozszczepowej twarzoczaszki. Genomowe DNA uzyskano z komórek nabłonkowych błony śluzowej jamy ustnej. Polimorfizm promotora genu MMP2 oznaczono metodą tetra-primer ARMS-PCR. Wyniki: Nie stwierdzono istotnych różnic w częstości występowania poszczególnych alleli w różnych typach rozszczepów. Częstość występowania genotypu CC w grupie dzieci z rozszczepem wargi i podniebienia była znacząco wyższa w porównaniu z grupą kontrolną (p = 0,005). Wniosek: Określenie polimorfizmu sekwencji promotorowych genów kodujących metaloproteinazy macierzy zewnątrzkomórkowej może przyczynić się do wyjaśnienia etiopatogenezy rozszczepu wargi i/lub podniebienia.

4

Potentially positive ageing-related variations of medial smooth muscle cells in the saphenous veins used as aortocoronary bypass grafts

84%

Perek B. , Malinska A. , Gasowski J. , Ostalska-Nowicka D. , Perek A. , Jemielity M. , Zabel M. , Nowicki M.

Folia Histochemica et Cytobiologica

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2016

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tom 54

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nr 2

5

The assessment of serum and diagnostic peritoneal lavage concentration of matrix metalloproteinase-2, matrix metalloproteinase-9, carbohydrate antigen 19-9, and carcinoembryonic antigen in patients with pancreatic cancer and chronic pancreatitis

84%

Dranka-Bojarowska D. , Lewinski A. , Lekstan A. , Gajda M. , Ciosek J. , Mrowiec S.

Journal of Physiology and Pharmacology

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2020

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tom 71

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nr 5

6

Effects of renin-angiotensin system inhibitors on fibrosis in patients with alcoholic chronic pancreatitis

84%

Madro A. , Kurzepa J. , Celinski K. , Slomka M. , Czechowska G. , Kurzepa J. , Kazmierak W. , Buszewicz G. , Ciesielka M. , Madro R.

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nr 1

7

In vitro effects of TCDD, PCB126 and PCB153 on estrogen receptors, caspases and metalloproteinase-2 mRNA expression in the chicken shell gland

84%

Hrabia A. , Lesniak A. , Sechman A.

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nr 3-4

8

Serum matrix metalloproteinase 2 and tissue inhibitor of matrix metalloproteinases 2 in esophageal cancer patients

84%

Groblewska M. , Mroczko B. , Kozlowski M. , Niklinski J. , Laudanski J. , Szmitkowski M.

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tom 50

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nr 4

9

Differential glomerular immunoexpression of matrix metalloproteinases MMP-2 and MMP-9 in idiopathic IgA nephropathy and Schoenlein-Henoch nephritis

84%

Danilewicz M. , Wagrowska-Danilewicz M.

Folia Histochemica et Cytobiologica

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2010

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tom 48

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nr 1

63-67

10

Expression of matrix metalloproteinase-2 mRNA in the chicken ovary in relation to follicle remodelling

84%

Lesniak A. , Hrabia A.

Folia Biologica

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2012

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tom 60

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nr 3-4

11

Matrix metalloproteinase-2 C-1306T promoter polymorphism and breast cancer risk in the Saudi population

84%

Saeed H. M. , Alanazi M. S. , Alshahrani O. , Parine N. R. , Alabdulkarim H. A. , Shalaby M. A.

Acta Biochimica Polonica

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2013

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tom 60

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nr 3

EN

Matrix metalloproteinase-2 (MMP-2) is an enzyme with proteolytic activity against matrix proteins, particularly basement membrane constituents. A single nucleotide polymorphism (SNP) at -1306, which disrupts a Sp1-type promoter site (CCACC box), displayed a strikingly lower promoter activity with the T allele. In the present study, we investigate whether this MMP-2 SNP is associated with susceptibility to breast cancer in the Saudi population. Ninety breast cancer patients and 92 age matched controls were included in this study. TaqMan Allele Discrimination assay and DNA sequencing techniques were used for genotyping. The results showed that, the frequency of MMP-2 CC wild genotype was lower in breast cancer patients when compared with healthy controls (0.65 versus 0.79). The homozygous CC (OR=2, χ2=5.36, p=0.02) and heterozygous CT (OR=1.98, χ2=4.1, p=0.04) showing significantly high risk of breast cancer in the investigated group. In conclusion our data suggest that the MMP-2 C-1306T polymorphism may be associated with increased breast cancer risk in the Saudi population.

12

Matrix metalloproteinases-2, -7 and tissue metalloproteinase inhibitor-1 expression in human endometrium

84%

Grzechocinska B. , Dąbrowski F. A. , Cyganek A. , Chlebus M. , Kobierzycki C. , Michalowski L. , Gornicka B. , Wielgos M.

Folia Histochemica et Cytobiologica

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2018

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tom 56

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nr 3

13

No correlation between pinopode formation and LIF and MMP2 expression in endometrium during implantation window

67%

Mikolajczyk M. , Skrzypczak J. , Wirstlein P.

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tom 49

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nr 4

14

Expression of matrix metalloproteinase-9 in the neoplastic and interstitial inflammatory infiltrate cells in gastric cancer

67%

Mroczko B. , Lukaszewicz-Zajac M. , Guzinska-Ustymowicz K. , Gryko M. , Czyzewska J. , Kemona A. , Kedra B. , Szmitkowski M.

Folia Histochemica et Cytobiologica

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2009

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tom 47

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nr 3

15

The role of matrix metalloproteinases (MMPs) and their inhibitors (TIMPs) in the development of esophageal cancer

67%

Groblewska M. , Siewko M. , Mroczko B. , Szmitkowski M.

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tom 50

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nr 1

16

Expression of superoxide dismutase and matrix metalloproteinase type 2 in diaphragm muscles of young rats

67%

Carmeli E. , Maor M. , Kodesh E.

Journal of Physiology and Pharmacology. Supplement

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2009

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tom 60

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nr 5

17

Clinical significance of serum levels of matrix metalloproteinase 2 (MMP-2) and its tissue inhibitor (TIMP-2) in gastric cancer

67%

Mroczko B. , Lukaszewicz-Zajac M. , Gryko M. , Kedra B. , Szmitkowski M.

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tom 49

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nr 1

18

Influence of IGF1, TGFbeta1, bFGF and G-CSF/M-CSF on the mRNA levels of selected matrix proteins, cytokines, metalloproteinase 3 and TIMP1 in rat synovial membrane cells

67%

Osiecka-Iwan A. , Moskalewski S. , Hyc A.

Folia Histochemica et Cytobiologica

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2016

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tom 54

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nr 3

19

Matrix metalloproteinase 2 and tissue inhibitor of matrix metalloproteinases 2 in the diagnosis of colorectal adenoma and cancer patients

67%

Groblewska M. , Mroczko B. , Gryko M. , Kedra B. , Szmitkowski M.

Folia Histochemica et Cytobiologica

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2010

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tom 48

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nr 4

20

Concentrations and activities of metalloproteinases 2 and 9 and their inhibitors (TIMPS) in chronic pancreatitis and pancreatic adenocarcinoma

67%

Lekstan A. , Lampe P. , Lewin-Kowalik J. , Olakowski M. , Jablonska B. , Labuzek K. , Jedrzejowska-Szypulka H. , Olakowska E. , Gorka D. , Filip I. , Dranka-Bojarowska D.

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2012

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tom 63

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nr 6