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1
Content available remote Tissue-specific effect of refeeding after short- and long-term caloric restriction on malic enzyme gene expression in rat tissues.
100%
Stelmanska E. , Korczynska J. , Swierczynski J.
Acta Biochimica Polonica
|
2004
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tom 51
|
nr 3
805-814
EN
Restricting food intake to a level below that consumed voluntarily (85%, 70% and 50% of the ad libitum energy intake for 3 or 30 days) and re-feeding ad libitum for 48 h results in an increase of malic enzyme (ME) gene expression in rat white adipose tissue. The increase of ME gene expression was much more pronounced in rats maintained on restricted diet for 30 days than for 3 days. The changes in ME gene expression resembled the changes in the content of SREBP-1 in white adipose tissue. A similar increase of serum insulin concentration was observed in all groups at different degrees of caloric restriction and refed ad libitum for 48 h. Caloric restriction and refeeding caused on increase of ME activity also in brown adipose tissue (BAT) and liver. However, in liver a significant increase of ME activity was found only in rats maintained on the restricted diet for 30 days. No significant changes after caloric restriction and refeeding were found in heart, skeletal muscle, kidney cortex, and brain. These data indicate that the increase of ME gene expression after caloric restriction/refeeding occurs only in lipogenic tissues. Thus, one can conclude that caloric restriction/refeeding increases the enzymatic capacity for fatty acid biosynthesis.
2
Content available Multiple isotope effects as a probe of the tartrate dehydrogenase-catalyzed oxidative decarboxylation of D-malate
100%
Karsten W. , Cook P.
Nukleonika
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2002
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tom Vol. 47,suppl.1
13-15
EN
A change in the dinucleotide reactant from NAD+ to the more oxidizing APAD+ in the malic enzyme reaction results in a change in the mechanism of oxidative decarboxylation of malate from stepwise to concerted [7]. In order to determine whether this is a phenomenon general to metal ion dependent â-hydroxyacid oxidative decarboxylases, tartrate dehydrogenase, which catalyzes a reaction diastereotopic to malic enzyme, was studied using the technique of multiple isotope effects. A primary deuterium isotope effect of 1.41 on V/Kmalate was measured, as well as a primary 13C-isotope effect of 1.0096. A decrease in the measured value of the 13C-isotope effect to 1.0078 is consistent with a stepwise mechanism, as observed for malic enzyme. The 13C-isotope effect with thioNAD+ also decreases from a value of 1.0053 using D-malate to 1.0009 using D-malate-2-D, consistent with stepwise oxidative decarboxylation with this alternative dinucleotide substrate. The data suggest that the change
3
Content available remote Effect of cadmium and glutathione on malic enzyme activity in brown shrimps (Crangon crangon) from the Gulf of Gdańsk
75%
Niedźwiecka N. , Mika A. , Białk-Bielińska A. , Stepnowski P. , Skorkowski E. F.
Oceanologia
|
2011
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tom No. 53 (3)
793-805
EN
The high level of cadmium in the abdominal muscle of the brown shrimp Crangon crangon is due to the serious pollution of the water in the Gulf of Gdańsk. The inhibition of malic enzyme (ME) activity by cadmium, and in consequence the reduced formation of NADPH, could interfere with cellular mechanisms for detoxifying the organism and reducing oxidative stress. The reduced glutathione (GSH) concentration in the abdominal muscle of C. crangon was calculated to be 5.8 mM. The objective of this study was to evaluate the part played by GSH in the effect of cadmium on the activity of NADP-dependent malic enzyme from abdominal muscles of brown shrimps. This enzyme is activated by certain divalent cations (Mg, Mn). The results demonstrate that cadmium inhibits ME activity from shrimp muscle, and that GSH and albumin can reduce this cadmium-inhibited NADP-dependent malic enzyme activity.
4
Isolation and some properties of malic enzyme from the abdomen muscle of Antarctic krill Euphausia superba
63%
Napierska D. , Skorkowski E. F.
Bulletin of the Sea Fisheries Institute
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2001
|
nr 1
5
Tissue-specific effect of refeeding after short- and long-term caloric restriction on malic enzyme gene expression in rat tissues
63%
Stelmanska E. , Korczynska J. , Swierczynski J.
Acta Biochimica Polonica
|
2004
|
tom 51
|
nr 3
EN
Restricting food intake to a level below that consumed voluntarily (85%, 70% and 50% of the ad libitum energy intake for 3 or 30 days) and re-feeding ad libitum for 48 h results in an increase of malic enzyme (ME) gene expression in rat white adipose tissue. The increase of ME gene expression was much more pronounced in rats main­tained on restricted diet for 30 days than for 3 days. The changes in ME gene expres­sion resembled the changes in the content of SREBP-1 in white adipose tissue. A sim­ilar increase of serum insulin concentration was observed in all groups at different degrees of caloric restriction and refed ad libitum for 48 h. Caloric restriction and refeeding caused on increase of ME activity also in brown adipose tissue (BAT) and liver. However, in liver a significant increase of ME activity was found only in rats maintained on the restricted diet for 30 days. No significant changes after caloric re­striction and refeeding were found in heart, skeletal muscle, kidney cortex, and brain. These data indicate that the increase of ME gene expression after caloric re- striction/refeeding occurs only in lipogenic tissues. Thus, one can conclude that calo­ric restriction/refeeding increases the enzymatic capacity for fatty acid biosynthesis.
6
Content available remote The effects of weight cycling on serum leptin levels and lipogenic enzyme activities in adipose tissue
51%
Kochan Z. , Karbowska J. , Swierczynski J.
|
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tom 57
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nr 6
115-127
EN
Weight cycling is one of the widely used weight reduction strategies; however, the adverse effects of this method include regaining significant amounts of weight. The molecular mechanisms underlying weight gain following cycles of dietary deprivation and refeeding are still poorly understood. One of the possibilities is that repeated loss and gain of weight may promote fat deposition in adipose tissue. To test this hypothesis we investigated serum leptin levels and lipogenic enzyme activities in white adipose tissue (WAT) of male Wistar rats during 12 days of ad libitum feeding following multiple cycles of alternating food deprivation and refeeding. Rats subjected to eight cycles of food deprivation and refeeding (MFR group) showed significantly decreased circulating leptin levels when compared with control rats (nearly 50% decrease in leptin levels, P < 0.01). Throughout 12 days of ad libitum feeding, serum leptin levels increased modestly but remained significantly (24%, P < 0.05) lower than control levels. Fatty acid synthase (FAS) and malic enzyme (ME) activities (chosen as representatives of enzymes directly involved in fatty acid synthesis) were found to be considerably higher in WAT of MFR rats refed for 3 days in comparison to control rats, and remained elevated even after 12 days of refeeding. These observations suggest that the elevation of lipogenic enzyme activities induced by multiple cycles of dietary deprivation followed by refeeding persists for several days, markedly increasing the lipogenic capacity of adipose tissue, which, accompanied by a decrease in circulating leptin levels, may promote weight gain.
7
Content available Alteration of plasma membrane NADPH oxidase and NADP-recycling enzymes in cucumber roots stressed with cadmium
51%
Jakubowska D. , Kabala K. , Reda M. , Janicka-Russak M.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
|
2013
|
tom 94
|
nr 2
8
Content available Effect of cadmium and glutathione on malic enzyme activity in brown shrimps (Crangon crangon) from the Gulf of Gdansk
51%
Niedzwiecka N. , Mika A. , Bialek-Bielinska A. , Stepnowski P. , Skorkowski E. F.
Oceanologia
|
2011
|
tom 53
|
nr 3
EN
The high level of cadmium in the abdominal muscle of the brown shrimp Crangon crangon is due to the serious pollution of the water in the Gulf of Gdańsk. The inhibition of malic enzyme (ME) activity by cadmium, and in consequence the reduced formation of NADPH, could interfere with cellular mechanisms for detoxifying the organism and reducing oxidative stress. The reduced glutathione (GSH) concentration in the abdominal muscle of C. crangon was calculated to be 5.8 mM. The objective of this study was to evaluate the part played by GSH in the effect of cadmium on the activity of NADP-dependent malic enzyme from abdominal muscles of brown shrimps. This enzyme is activated by certain divalent cations (Mg, Mn). The results demonstrate that cadmium inhibits ME activity from shrimp muscle, and that GSH and albumin can reduce this cadmium-inhibited NADP-dependent malic enzyme activity.
9
Changes in the activities of carbon metabolizing enzymes with pod development in lentil [Lens culinaris L.]
51%
Chopra J. , Kaur N. , Gupta A. K.
Acta Physiologiae Plantarum
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2003
|
tom 25
|
nr 2
10
Comparison of short-term cadmium poisoning in the shrimp Crangon crangon from the Baltic Sea and the shrimp Palaemon serratus from the Atlantic Ocean with cadmium bioaccumulation and malic enzyme activity in abdomen muscle
44%
Napierska D. , Thebault M. T. , Pempkowiak J. , Skorkowski E.
Oceanologia
|
1997
|
tom 39
|
nr 2
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