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nr 2
EN
A study on reduced tillage effect on the micronutrients (B, Cu, Fe, Mn, Mo, Zn) content in two horizons (0-10 cm and 10-20 cm) of the arable layer of sandy soil in the maize monoculture was carried out. Micronutrient supply of maize plants and yielding was also tested. An increase of soil acidity in the 0-10 cm horizon of soil under the reduced tillage was stated. It was accompanied by the increased micronutrient content in comparison with both the deeper, 10-20 cm, horizon under the same two systems and the respective contents in the topmost horizon (0-10 cm deep) under the conventional tillage. This exerted an influence on micronutrient content in plants. A significant increase in the content of Cu and Zn alongside a decrease in the amounts of B, Mn, Fe and Mo in vegetative organs and grain of maize were observed under no-plough tillage. Significantly depressed maize grain yields occurred in the objects under no-plough tillage suggests that the changes of micronutrients contents in soil and maize plants could be one of the reasons. The results obtained indicate the necessity of microelement fertilization of maize monoculture especially when the reduced tillage is applied.
PL
W badaniach określano wpływ uproszczeń uprawy roli na zawartość mikroelementów (B, Cu, Fe, Mn, Mo, Zn) w dwóch poziomach warstwy ornej gleby lekkiej (0-10 cm i 10-20 cm) w monokulturze kukurydzy. Oceniano także plonowanie oraz zaopatrzenie roślin kukurydzy w mikroelementy. Stwierdzono wzrost kwasowości w warstwie powierzchniowej gleby upraw uproszczonych (0-10 cm), któremu towarzyszyła zwiększona koncentracja mikroelementów, zarówno w stosunku do warstwy głębszej (10-20 cm) tych systemów uprawy jak i warstwy 0-10 cm uprawy tradycyjnej (orkowej). Wywarło to wpływ na zawartość badanych mikroelementów w roślinach. Stwierdzono istotny wzrost koncentracji Cu i Zn oraz spadek B, Mn, Fe i Mo w częściach wegetatywnych i ziarnie kukurydzy z upraw bezorkowych w stosunku do uprawy tradycyjnej (orkowej). Istotne spadki plonowania ziarna kukurydzy w obiektach z uprawą powierzchniową i siewem bezpośrednim, wskazują, że ich przyczyną mogły być między innymi zmiany zawartości mikroelementów w glebie i roślinach. Uzyskane wyniki wskazują na potrzebę nawożenia mikroelementami kukurydzy uprawianej w monokulturze na glebie lekkiej, zwłaszcza, gdy stosowane są uproszczenia uprawy roli.
EN
Ability of five strains of Trichoderma pseudokoningii (antagonists) to suppress radial growth of Fusarium verticillioides (Sacc.) Nirenberg (=Fusarium moniliforme Sheldon) was examined i n vi tro. These were T. pseudokoningii strain1 (IMI 380933), strain2 (IMI 380937), strain3 (IMI 3809 39), strain4 (IMI 380940) and strain5 (IMI 380941). Each strain was paired with pathogen by inoculating at opposite ends of 9 cm petri plates using three pairing methods. Gradings were assigned to varied growth inhibition of pathogen by antagonists and analysed using GLM procedure (SAS). Growth suppressionof F. verticillioides by all strains of T. pseudokoningii was significantly different (R2 =0.98, p=0.05) from control in all pairing methods. It differed significantly (p>0.0003) among the strains in all pairing methods. Growth suppression also differed significantly among (p>0.0001) and within (p>0.018) pairing methods. Growth suppression was best when antagonists were inoculated before pathogen. Suppression mechanisms include mycoparasitism and competition for space and nutrients. T. pseudokoningii strains 3 and 4 had the best (p=0.05) growth suppressionof F. verticillioides and could be used as biocontrol agents for endophytic F. verticillioides in maize plant. This experiment was conducted in the search for resident microorganisms that might be capable of checking F. verticillioides withinmaize plant by competitive exclusion in subsequent experiments.
PL
W warunkach in vitro zbadano aktywność pięciu szczepów Trichoderma pseudokoningii w hamowaniu wzrostu fitopatogennego szczepu Fusarium verticillioides – sprawcy fuzaryjnego uwiądu kukurydzy. Każdy szczep antagonisty wysiewano w szalkach Petriego z grzybem chorobotwórczym i analizowano ich wzrost zgodnie z metodyką GLM(SAS). Najsilniejsze hamowanie wzrostu F. verticillioides stwierdzono, gdy antagonista był inokulowany jako pierwszy. Szczep nr 3 T. pseudokoningii wykazał najsilniejsze działanie i może być przydatny w biologicznej ochronie kukurydzy przed fuzaryjnym uwiądem.
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tom 60
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nr 4
EN
The objective of the performed investigations was to isolate pathogenic fungi from contaminated maize cobs, to assess the appearance of maize cob fusariosis and to determine grain contamination with deoxynivalenol in the cultivation of genetically modified maize containing a gene resistance against European corn borer (Ostrinia nubilalis Hbn) as well as selected non-modified cultivars. The plant material comprised the following genetically modified maize cultivar: DKC 3421 YG (MON 810) and non-modified cultivars obtained from Smolice Plant Breeding Ltd., IHAR Group: Junak (FAO 210–220), Prosna (FAO 220), SMH (FAO 230), Baca (FAO 220). Prior to harvesting, the occurrence of maize cob fusariosis was determined in the 89 (BBCH) developmental ripening stage. Microbiological assessment was carried out on grains selected from cobs characterized by various pathological symptoms. In 2008, a total of 133 isolates was obtained from the examined samples of infected maize plants, of which 51 isolates were species-identified, while in 2009, the total of 123 isolates were determined, of which 63 were species-identified. In both experimental years, the majority of isolates contained fungi from the Fusarium genus. The performed analysis of mean levels of cob contamination by fusarioses revealed that DKC 3421 YG (MON 810) and SMH (FAO 230) cultivars showed the smallest levels of contamination as well as the lowest percent of cob contamination per plant, while Junak (FAO 210–220) and Baca (FAO 220) cultivars were characterized by the highest degree of contamination. The lowest deoxynivalenol concentrations were determined in years 2008 and 2009 in the case of the DKC 3421 YG (MON 810) cultivar, whereas Prosna (FAO 220) cultivar was characterized by the highest deoxynivalenol concentration.
EN
Pot experiments were conducted to evaluate the possible roles of nitrogen fixation and/or enhanced mineral uptake by Azospirillum lipoferum and Bacillus polymexa inoculation in improving salt tolerance of maize plants. Plants were inoculated and grown under salt stress (osmotic potential: -0.3, -0.6, -0.9 and -1.2 Mpa). Both microorganisms were able to fix nitrogen up to -0.9 Mpa salinity level accompanied with increased total N-yield compared with the control plants. In order to investigate the role of bacterial inoculation on enhanced mineral uptake, the growth and some physiological parameters of inoculated plants were compared with plants fertilized by K and P foliar application. Plant inoculation with the N₂-fixers or plant spraying with KH₂PO₄ resulted in an increase in fresh and dry matter as well as water content of plants. Treated plants exhibited changed plant mineral content which was associated with increased Mg/K and decreased P/K, Ca/K and Na/K ratios. This was accompanied by accumulation of soluble sugars, amino acids in shoots and roots of plants resulting in a concomitant increase in the osmotic potential of the cell sap as a possible mechanism of adaptation to salinity.
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