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EN
Ludwik Karol Teichmann significantly contributed to the creation of modern techniques in the anatomical preparations. He was, next to Joseph Hyrtl, the most versatile among the anatomical preparators in the second half of the 19th century, successfully introducing modifications to existing methods, as well as striving for independent solutions in this field. His precision in performance, transparency and sustainability of the whole brain preparations, excellent osteological preparations, including small bones and cartilage, evoked and still evoke high admiration. He made his name, however, with preparations obtained by means of injection and corrosion techniques. The application of these techniques in the lymphatic system’s study, both the physiologically proper and the pathologically changed, earned Teichmann a permanent position in the history of anatomy. The developed by Teichmann mass for cold injections (the so-called Teichmann’s cold mass) revolutionized the macro-and microscopic preparatory of that time, thus opening great new research perspectives still widely used during the interwar period.
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Content available remote Ludwik Karol Teichmann jako preparator
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EN
Ludwik Karol Teichmann significantly contributed to the creation of modern techniques in the anatomical preparations. He was, next to Joseph Hyrtl, the most versatile among the anatomical preparators in the second half of the 19th century, successfully introducing modifications to existing methods, as well as striving for independent solutions in this field. His precision in performance, transparency and sustainability of the whole brain preparations, excellent osteological preparations, including small bones and cartilage, evoked and still evoke high admiration. He made his name, however, with preparations obtained by means of injection and corrosion techniques. The application of these techniques in the lymphatic system’s study, both the physiologically proper and the pathologically changed, earned Teichmann a permanent position in the history of anatomy. The developed by Teichmann mass for cold injections (the so-called Teichmann’s cold mass) revolutionized the macro-and microscopic preparatory of that time, thus opening great new research perspectives still widely used during the interwar period.
EN
Localization and morphological features of lymphatic vessels leaving tissues of uterine horns and running through the broad ligament area were studied in mature cows after filling the lumen of the each lymphangion with varicoloured masses, mainly Microfil. The study has revealed that each branch of the lymphatic vessels emerged from both sides of the uterus and then formed double-layered networks, dorsal and ventral in the area of the mesometrium. The lymphatic branches under the uterus are very numerous and consist of enlarged precollector lymphangions. At the level of the ovary, the branches of vessels in both layers interweave each other forming the common pathway suggesting that lymph leaving the bovine uterus can be mixed in branches which are formed by elongated lymphangions running to lymphatic nodes.
EN
Larvae of the parasitic nematode Trichinella spiralis migrate via the bloodstream or the lymphatic system to the skeletal muscle cells where they induce multiple alterations in the intracellular environment leading to the formation of nurse cells. The “nurse cell-T. spiralis larva” complex is composed of a transformed fragment of a skeletal muscle cell and the wall of the larva. The pathological process responsible for the formation of this complex, known as basophilic transformation, is essential for the development of T. spiralis larvae, but it still not known how newborn larvae penetrate the transformed fragment of the muscle cell. In this study, we aimed to characterize the ultrastructure of the region of the nurse cell in direct contact with the larval wall, after one and two weeks of T. spiralis infection in mice. For this purpose, a transmission electron microscope fitted with a goniometer was used to make observations of samples tilted at an angle of ±40° relative to the axis of the electron beam. Examination of electron micrographs revealed the continuity of the nurse cell membrane adjacent to the larval surface and the presence of a large quantity of glycogen particles close to the inner surface of this membrane. Our results showed that death of the T. spiralis larvae was associated with destruction of the contact region between the larval wall and the adjacent surface of the nurse cell. We conclude that the T. spiralis larva does not penetrate the nurse cell, but a morphological “junction” is formed between the larval wall and the cell membrane.
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