Wyniki wyszukiwania - Biblioteka Nauki

1

Secretion of proinflammatory cytokines by normal human melanocytes in response to lipopolysaccharide

100%

Tam I. , Stępień K.

Acta Biochimica Polonica

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2011

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tom 58

|

nr 4

507-511

EN

A large body of evidence suggests that epidermal melanocytes are an integral part of the skin immune system and can be considered immunocompetent cells. Recently, it has been reported that human melanocytes constitutively express Toll-like receptors and may be involved in the induction of several inflammatory cytokines. In the study the secretion of IL-1β, IL-6 and TNF-α by cultured normal melanocytes was investigated after stimulation with lipopolysaccharide. LPS increased the secretion of IL-1β in a dose-dependent manner. IL-1β stimulated release of IL-6 and TNF-α by melanocytes, whereas LPS activated production of TNF-α, but not of IL-6. These observations indicate that LPS can participate in the regulation of cytokine activity in normal human melanocytes and suggest that cytokines released by melanocytes could affect melanocytes themselves or/and other cells of the epidermis.

2

The Structure of the O-Specific Polysaccharide of the Lipopolysaccharide from Acinetobacter Strain 44 (DNA Group 3)

100%

Vinogradov E. , Brade L. , Brade H. , Holst O.

Polish Journal of Chemistry

|

2005

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tom Vol. 79 / nr 2

267-273

EN

Extraction of dry bacteria of Acinetobacter strain 44 (DNA group 3) by phenol/water gave a polymer that was identified by means of serological studies as S-form lipopolysaccharide (LPS). Mild acetic acid hydrolysis degraded the O-specific polysaccharide, thus, its structure was investigated by compositional analyses andNMRspectroscopy after de-O-acylation and Smith degradation of the LPS. The structure of the O-specific polysaccharides was 3)-alfa-L-Rhap-(1_2)-alfa-L-Rhap-(1--3)-beta-D-GlcpNAc-(1--3)-alfa-L-Rhap-(1-- 2 _ 1 alfa-L-Rhap-(1--4)-beta-D-GlcpA-(1--2)-alfa-L-Rhap After immunization of BALB/c mice with Acinetobacter strain 44, monoclonal antibody S48-26 (IgG3 isotype) was obtained which reacted in Western blot with this LPS and characterized it as S-form.

3

Repeating Unit Structure of Enterobacter sakazakii ZORB A 741 O-Polysaccharide

100%

Szafranek J. , Czerwicka M. , Kumirska J. , Paszkiewicz M. , Łojkowska E.

Polish Journal of Chemistry

|

2005

|

tom Vol. 79 / nr 2

287-295

EN

The O-specific polysaccharide from Enterobacter sakazakii cell was isolated and structurally characterized. Lipopolysaccharide (LPS) was obtained from cell mass by hot phenol- water extraction procedure. Mild acid hydrolysis followed by gel filtration provided pure O-antigen (OPS). Two-stage sugar analysis detected tyvelose, rhamnose and galactose in the molar ratio of 1:1:2, and their linkages were established by means of methylation analysis. Sugar configurations, D or L, were determined by gas-liquid chromatography on an achiral liquid phase for (S)-(+)-2-butyl glycosides. D configuration was determined for galactose and 3,6-dideoxy-mannose (tyvelose), but L for rhamnose. Repeating unit structure was deduced by analysis of 1H and 13C NMR spectra. 1H and 13C NMR resonances have been assigned by homonuclear (COSY, TOCSY) and heteronuclear (HSQC, HMBC) correlations spectra. Anomeric configurations were determined from anomeric proton chemical shifts and 3JH1-H2 and JC-H coupling constants. Sugar sequences were established from comparisons of specific carbon chemical shifts with those in literature, two-dimensional nuclear Overhauser effect spectroscopy (NOESY), and heteronuclear multiple-bond correlation experiments (HMBC). The repeating unit structure of Enterobacter sakazakii was found to be as: alfa-Tyvp _2 _3)-alfa-L-Rhap-(1--3)-alfa-D-Galp-(1_3)-alfa-D-Galp-(1_ _6 O-Ac

4

Characterization of epitope specificity of Proteus penneri 7 lipopolysaccharide core region

100%

Palusiak A. , Sidorczyk Z.

Acta Biochimica Polonica

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2010

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tom 57

|

nr 4

529-532

EN

To extend the knowledge on the fragments of Proteus penneri lipopolysaccharide core regions, which determine the cross-reactions with specific antibodies, serological studies were performed by use of P. penneri 7 core-specific antiserum and Proteus sp. lipopolysaccharides. Different reactivity of the tested antiserum with three groups of antigens suggested differences in their core regions' epitope specificity. Comparing the results of the serological investigations with the previously determined structures of the core regions of the tested P. penneri lipopolysaccharides allowed distinguishing two potential tri- and tetrasaccharide epitopes and a third fragment which could not be determined precisely.

5

Selective brain cooling in the ox during E.coli LPS induced fever

100%

Lachowski A. , Chesy G.

Bulletin of the Veterinary Institute in Puławy

|

1994

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tom 38

|

nr 2

6

Chemical characterization of effective and ineffective strains of Rhizobium leguminosarum bv.viciae

100%

Izmailov S. F. , Zhiznevskaya G. Y. , Kosenko L. V. , Troitskaya G. N. , Kudryavtseva N. N. , Borodenko L. I. , Dubrovo P. N. , Russa R. , Pietras H. , Lorkiewicz Z.

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nr 4

EN

Chemical composition of lipopolysaccharide (LPS) isolated from an effective (97) and ineffective (87) strains of R. l. viciae has been determined. LPS preparations from the two strains contained: glucose, galactose, mannose, fucose, arabinose, heptose, glucosamine, galactosamine, quinovosamine, and 3-N-methyl-3,6-dideoxyhexose, as well as glucuronic, galacturonic and 3-deoxyoctulosonic acid. The following fatty acids were identified: 3-OH 14:0, 3-OH 15:0, 3-OH 16:0, 3-OH 18:0 and 27-OH 28:0. The ratio of 3-OH 14:0 to other major fatty acids in LPS 87 was higher that in LPS 97. SDS/PAGE profiles of LPS indicated that, in lipopolysaccharides, relative content of S form LPS I to that of lower molecular mass (LPS II) was much higher in the effective strain 97 than in 87. All types of polysaccharides exo-, capsular-, lipo, (EPS, CPS, LPS, respectively) examined possessed the ability to bind faba bean lectin. The degree of affinity of the host lectin to LPS 87 was half that to LPS 97. Fatty acids (FA) composition from bacteroids and peribacteroid membrane (PBM) was determined. Palmitic, stearic and hexadecenoic acids were common components found in both strains. There was a high content of unsaturated fatty acids in bacteroids as well as in PBM lipids. The unsaturation index in the PBM formed by strain 87 was lower than in the case of strain 97. Higher ratio of 16:0 to 18:1 fatty acids was characteristic for PMB of the ineffective strain.

7

Characterization of two lipopolysaccharide types isolated from Rhizobium galegae

100%

Rasanen L. A. , Russa R. , Urbaniak T. , Choma A. , Mayer H. , Lindstrom K.

Acta Biochimica Polonica

|

1997

|

tom 44

|

nr 4

EN

Lipopolysaccharides (LPS) of Rhizobium galegae, a symbiotically nitrogen-fixing species of root-nodule bacteria, were isolated by the phenol-water method from strain HAMBI 1461, the LPS of which resembled enterobacterial smooth type LPS, and from strains HAMBI 1174 and HAMBI 1208, the LPSs of which resembled rough type LPS. The results of PAGE analysis of LPSs, Bio-Gel P2 gel filtration of polysaccharide fractions and the presence of deoxysugars and 4-O-methyl-deoxysugar both in the rough and smooth LPSs suggested that rough LPS contained a short O-antigenic polysaccharide for which we propose the name short O-chain LPS. Accordingly, the smooth LPS is called long O-chain LPS. Despite of the differences in the structure of LPS of R. galegae, all strains were equally effective in nodulating their hosts. The short O-chain LPS of R. galegae showed many features similar to those of phylogenetically related agrobacteria.

8

Proteus mirabilis RMS 203 as a new representative of the O13 Proteus serogroup

88%

Palusiak A. , Siwińska M. , Zabłotni A.

Acta Biochimica Polonica

|

2015

|

tom 62

|

nr 4

691-695

EN

The unique feature of some Proteus O-polysaccharides is occurrence of an amide of galacturonic acid with Nε-[(S/R)-1-Carboxyethyl]-l-lysine, GalA6(2S,8S/R-AlaLys). The results of the serological studies presented here, with reference to known O-antigens structures suggest that GalA6(2S,8S/R-AlaLys) or 2S,8R-AlaLys contribute to cross-reactions of O13 Proteus antisera, and Proteeae LPSs. It was also revealed that the Proteus mirabilis RMS 203 strain can be classified into the O13 serogroup, represented so far by two strains: Proteus mirabilis 26/57 and Proteus vulgaris 8344. The O13 LPS is a serologically important antigen with a fragment common to LPSs of different species in the Proteeae tribe.

9

Microbiome impact on metabolism and function of sex, thyroid, growth and parathyroid hormones

88%

Kunc M. , Gabrych A. , Witkowski J.

Acta Biochimica Polonica

|

2016

|

tom 63

|

nr 2

189-201

EN

Commensal bacteria and their genes associated with host are known as microbiome. In recent years, microbial influence on host endocrine system has been under detailed investigation. The role of microbiome in the pathogenesis of insulin resistance and obesity, the function of hypothalamic-pituitary-adrenal axis and secretion of hormones regulating appetite is well described in world literature. In this article we discuss poorly reviewed issues: the microbiome role in modulation of non-peptide (sex and thyroid) and peptide (growth hormone and parathyroid hormone) functions. Understanding complex bidirectional relations between host endocrine system and bacteria is of fundamental importance to understanding microbial impact on host reproduction, risk of endocrine-related cancers, pathogenesis of non-thyroidal illness syndrome, growth failure in children and hormonal changes during chronic kidney disease. This article also highlights effects of dietary compounds on microbiome composition and bacterial enzymes activity, and thus host hormonal status.

10

Detection of IL-1β, IL-6 and TNF-αin Sprague-Dawely rats’ atrophic thymus induced by lipopolysaccharide

88%

Zhong Y. B. , Zhang X. L. , Lv M. Y. , Hu X. F. , Li Y.

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tom 21

|

nr 3

11

The amide of galacturonic acid with lysine as an immunodominant component of the lipopolysaccharide core region from Proteus penneri 42 strain

88%

Palusiak A. , Maciejewska A. , Ługowski C. , Różalski A.

Acta Biochimica Polonica

|

2014

|

tom 61

|

nr 1

129-132

EN

Most Proteus lipopolysaccharides (LPSs) contain uronic acids or their amides with different amino acids, which together with other negatively charged components account for the acidic character of such LPS molecules. Previous studies have shown the significance of an amide of galacturonic acid with lysine [D-GalA(L-Lys)] for serological specificity of O-antigens from few P. mirabilis strains. In this work, the immunodominant role of GalALys was indicated for the P. penneri 42 LPS core region. The studies also showed the serological identity of core oligosaccharides from P. penneri 42 (O71), P. mirabilis 51/57 (O28) and R14/S1959 strains.

12

Dose-effect relationship on anti-inflammatory activity on LPS induced RAW 264.7 cells and antioxidant activity of rutin in vitro

88%

tian c. , guo y. , chang y. , zhao j. , cui c. , liu m.

Acta Poloniae Pharmaceutica - Drug Research

|

2019

|

tom 76

|

nr 3

511-522

EN

Rutin, as a common flavonol glycoside, exists abundantly in many plants and exhibits various nutritional and pharmacological function. Current study focused on the dose-effect relationship of rutin on the anti-inflammatory and antioxidant activities in vitro against LPS-stimulated RAW 264.7 cells and free radicals or ferric-ion. This study evaluated the anti-inflammatory activity by observation cell morphological and determination of the content of NO, TNF-α, IL-1β and IL-6, and the phagocytic activity in LPS induced RAW 264.7 cells; and revealed the antioxidant activities via assay of DPPH radical scavenging capacity, ABTS radical scavenging capacity, and reducing power. The study indicated that 5, 10, 20, 50 and 100 μM of rutin all exhibited a better anti-inflammatory activity, in especial 50 and 100 μM. Meanwhile, it exerted a stronger antioxidant activities than BHT, and equivalent to VC at the same concentration. Combined, the results suggested that rutin will be a potential and important adjuvant in treatment of inflammatory diseases and oxidative stress.

13

Central and peripheral neural aspects of gastroprotective and ulcer healing effects of lipopolysaccharides

88%

Konturek P. C. , Brzozowski T. , Meixner H. , Ptak A. , Hahn E. G. , Konturek S. J.

Journal of Physiology and Pharmacology

|

2001

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tom 52

|

nr 4,1

EN

Lipopolysaccharides (LPS) are major components of the outer membrane of Gram-- negative bacteria playing a central role as potent endotoxins in the pathogenesis of endotoxic shock. Although large amounts of endotoxin may produce hemorrhagic lesions in the stomach, the possible gastroprotective effect of central or peripheral LPS against the acute gastric lesions has not been extensively studied. The aim of the present study was to compare the effect of intracerebroventricular (i.c.v.) and parenteral (i.p.) injection of LPS against gastric lesions induced by 100% ethanol. Male Wistar rats were treated either with a) vehicle (control); b) E-coli-LPS in various concentrations (1—10 µg/kg i.c.v or 0.1—40 mg/kg i.p.) followed 30 min later by 100% ethanol. The effects of pretreatment with nonselective inhibitor of nitric oxide synthase (L-NAME, 20 mg/kg i.g.) or selective inhibitor of inducible nitric oxide synthase, L-NIL (30 mg/kg i.g) on the gastroprotection induced by LPS was investigated. One hour after ethanol application, the gastric blood flow (GBF) and the area of gastric lesions were determined. In addition, the mucosal expression of iNOS, cNOS and leptin was assessed using RT-PCR. LPS applied i.c.v. or i.p. dose dependently reduced gastric lesions induced by ethanol and this effect was similar to that observed after the administration of NO donor (SNAP). LPS-induced protection was significantly abolished by L-NAME and significantly attenuated by the selective inhibitor of iNOS (L-NIL). The expression of cNOS was detected in vehicle treated gastric mucosa and did not change after LPS administration. iNOS was not detectable in intact mucosa but its expression dose-dependently increased after the LPS administration. The i.c.v. administration of LPS did not upregulate further the iNOS expression, and dose-dependently inhibited the leptin mRNA expression in gastric mucosa. We conclude that LPS applied centrally or peripherally protects gastric mucosa against ethanol-induced damage through an increase in gastric microcirculation mediated by NO due to overexpression of iNOS. Transcriptional downregulation of leptin in gastric mucosa is probably due to the increased leptin release induced by the intracerebroventricular application of lipopolysaccharide.

14

Preparation of endotoxin-free bacteriophages

88%

Boratynski J. , Syper D. , Weber-Dabrowska B. , Lusiak-Szelachowska M. , Pozniak G. , Gorski A.

Cellular and Molecular Biology Letters

|

2004

|

tom 09

|

nr 2

EN

Bacteriophages (phages) are bacterial viruses that interact with bacterial walls and invade bacterial cells. Moreover, they disturb bacterial metabolism and lead to bacteria lysis. In the case of Gram-negative bacteria crude phage cultures, apart from the phages themselves, the bacterial debris, bacterial proteins and nucleic acids contain endotoxins. These endotoxins (lipopolysaccharides) posses a high degree of toxicity in vitro and in vivo, and their removal is essential for safety in antibacterial bacteriophage therapy. An effective, scaleable purification of bacteriophages from endotoxins was accomplished by sequential ultrafiltration through polysulfone membrane (30 nm) followed by chromatography on sepharose 4B and Matrex Cellulofine Sulfate. The phage fraction after gel filtration chromatography routinely contained endotoxins in the 150-2500 EU/mL range. The procedure yielded bacteriophages contaminated with as little as 0.4-7 EU/ml (Limulus assay). This value lies within the permitted level for intravenous applications (5 EU/kg/h by European Pharmacopoeia, 1997)

15

Role of prostaglandins and nitric oxide in the lipopolysaccharide-induced ACTH and corticosterone response

88%

Gadek-Michalska A. , Bugajski J.

Journal of Physiology and Pharmacology

|

2004

|

tom 55

|

nr 3

EN

This study was designed to determine the role of endogenous prostaglandins (PG) and nitric oxide (NO) in the lipopolysaccharide (LPS)-induced ACTH and corticosterone secretion in conscious rats. LPS (0.5 and 1 mg/kg) given i.p. stimulated the hypothalamic-pituitary-adrenocortical (HPA) activity measured 2 h later. A non-selective cyclooxygenase inhibitor indomethacin (10 mg/kg i.p.), piroxicam (2 mg/kg i.p.), a more potent antagonist of constitutive cyclooxygenase (COX-1) and compound NS-398 (2 mg/kg i.p.), a selective inhibitor of inducible cyclooxygenase (COX-2) given 30 min before LPS (1 mg/kg i.p.) significantly diminished both the LPS-induced ACTH and corticosterone secretion. COX-2 blocker was the most potent inhibitor of ACTH secretion (72.3%). Nw-nitro-L-arginine methyl ester (L-NAME 2 and 10 mg/kg i.p.), a non-selective nitric oxide synthase (NOS) blocker given 15 min before LPS did not substantially alter plasma ACTH and corticosterone levels 2 h later. Aminoguanidine (AG 100 mg/kg i.p.), a selective inducible nitric oxide synthase (iNOS) inhibitor, considerably enhanced ACTH and corticosterone secretion induced by a lower dose (0.5 mg/kg) of LPS and did not significantly alter this secretion after a larger dose (1 mg/kg) of LPS. L-NAME did not markedly affect the indomethacin-induced inhibition of ACTH and corticosterone response. By contrast, aminoguanidine abolished the indomethacin-induced reduction of ACTH and corticosterone secretion after LPS. These results indicate an opposite action of PG generated by cyclooxygenase and NO synthesized by iNOS in the LPS-induced HPA-response.

16

Immunochemical studies on R mutants of Yersinia enterocolitica O:3

88%

Radziejewska-Lebrecht J. , Skurnik M. , Shashkov A. S. , Brade L. , Rozalski A. , Bartodziejska B. , Mayer H.

Acta Biochimica Polonica

|

1998

|

tom 45

|

nr 4

EN

Three mutants of Yersinia enterocolitica O:3, namely: YeO3-R1, YeO3-RfbR7 and YeO3-c-trs8-R were classified on the basis of sodium dodecyl sulphate/polyacrylamide gel electrophoresis (SDS/PAGE) profile of isolated lipopolysaccharides (LPS) as belonging to the Ra- (the first) and the Rc-type (the other two mutants). Methylation analysis, in addition to 13C and 1H NMR studies of purified core oligosaccharides revealed structures similar to those established previously for the full core of Y. enterocolitica O:3 in the case of the Ra mutant, and identical to that reported for the Rc mutant Ye75R, in the case of the two other mutants. The O-specific sugar, 6d-l-altrose, which forms a homopolymeric O-chain, was present in small amounts in all three LPS preparations, as well as in the core oligosaccharide preparations along with the Ra and the Rc sugars, characteristic of the Y. enterocolitica O:3 core. This result is in line with genetic data, indicating that it is the inner core region which is the receptor for the O-specific chain in Y. enterocolitica O:3. This region seems likewise to be the anchoring region for the enterobacterial common antigen (ECA), as shown by SDS/PAGE/Western blot analysis with monoclonal antibodies against ECA. In addition, we also demonstrated that the Ye75R mutant Rc and its parental strain Ye75S, both were ECA-immunogenic strains. So far, ECA-immunogenic strains, i.e. those with LPS-linked ECA, were only identified in E. coli mutants of the R1, R4 and K-12 serotype.

17

Level of interleukin-1beta and clinical signs after endotoxin injection in stallions

88%

Danek J.

Bulletin of the Veterinary Institute in Puławy

|

2002

|

tom 46

|

nr 2

18

Carvedilol and adrenergic agonists suppress the lipopolysaccharide-induced NO production in RAW 264.7 macrophages via the adrenergic receptors

75%

Pekarova M. , Kralova J. , Kubala L. , Ciz M. , Papezikova I. , Macickova T. , Pecivova J. , Nosal R. , Lojek A.

|

tom 60

|

nr 1

143-150

EN

The interaction of adrenergic agonists and/or antagonists with the adrenergic receptors expressed on immunologically active cells including macrophages plays an important role in regulation of inflammatory responses. Our study investigated the effects of carvedilol, a unique vasodilating b-adrenergic antagonist, and endogenous adrenergic agonists (adrenalin, noradrenalin, and dopamine) and/or antagonists (prazosin, atenolol) on lipopolysaccharide-stimulated nitric oxide (NO) production from murine macrophage cell line RAW 264.7. The production of NO was determined as the concentration of nitrites in cell supernatants (Griess reaction) and inducible nitric oxide synthase (iNOS) protein expression (Western blot analysis). Scavenging properties against NO were measured electrochemically. Carvedilol in a concentration range of 1, 5, 10 and 25 µM inhibited iNOS protein expression and decreased the nitrite concentration in cell supernatants. Adrenalin, noradrenalin or dopamine also inhibited the iNOS protein expression and the nitrite accumulation. Prazosine and atenolol prevented the effect of both carvedilol and adrenergic agonists on nitrite accumulation and iNOS expression in lipopolysaccharide-stimulated cells. These results, together with the absence of scavenging properties of carvedilol against NO, imply that both carvedilol and adrenergic agonists suppress the lipopolysaccharide-evoked NO production by macrophages through the activation and modulation of signaling pathways connected with adrenergic receptors.

19

Lack of TCRalpha beta plus CD8plus and TCRgamma delta plus lymphocytes ameliorates LPS induced orchitis in mice - preliminary histological observations

75%

Sliwa L. , Macura B. , Majewska-Szczepanik M. , Szczepanik M.

Folia Biologica

|

2014

|

tom 62

|

nr 1

20

Rhizobium strains differ considerably in outer membrane permeability and polymyxin B resistance

75%

Komaniecka I. , Zamłyńska K. , Zan R. , Staszczak M. , Pawelec J. , Seta I. , Choma A.

Acta Biochimica Polonica

|

2016

|

tom 63

|

nr 3

517-525

EN

Six rhizobium (Rhizobium leguminosarum bv. Trifolii TA1, Sinorhizobium meliloti 1021, Mesorhizobium huakuii IFO 15243T, Ochrobactrum lupini LUP 21T, Bradyrhizobium japonicum USDA110 and B. elkanii USDA 76) and two Escherichia coli strains (E. coli ATCC 25922 and E. coli HB 101) were compared in respect to polymyxin B and EDTA resistance, as well as bacterial outer membrane (OM) permeability to a fluorescent hydrophobic agent (N-phenyl-1-naphthylamine - NPN). TEM (Transmission Electron Microscopy) and a microbial test demonstrated that all the rhizobia were much more resistant to polymyxin B in comparison with E. coli strains. EDTA and polymyxin B enhance permeability of B. japonicum and O. lupini OM. Other rhizobia incorporated NPN independently of the presence of membrane-deteriorating agents; however, the level of fluorescence (measured as NPN absorption) was strain dependent.