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EN
Changes in the bioaccumulation of 2,2',4,4'-tetrabromodiphenyl ether (BDE-47) in the marine alga Heterosigma akashiwo (Raphidophyceae) were examined for different concentrations of nitrate (0, 128, and 512 μmol dm-3) and phosphate (0, 8, and 32 μmol dm-3) in the semi-continuous culture with 20% renewal rate. The BDE-47 content per cell and per culture, as well as the accumulated percentage of available BDE-47, presented a significant decreasing trend with the increase in nitrate and phosphate concentrations. The N-0 (4.0 × 10^-6 ng cell-1) and P-0 (5.8 × 10^-6 ng cell-1) treatments had significantly higher BDE-47 content per cell than other treatments. In comparison, the difference in BDE-47 per algal culture and accumulated percentage between the nitrate treatments or phosphate treatments was not as obvious as the BDE-47 content per cell. BDE-47 per cell presented significantly negative correlation with nitrate and phosphate concentrations, and the accumulated BDE-47 was in positive correlation with lipid content. log BAFlip for BDE-47 in H. akashiwo ranged from 6.70 to 7.25. The results of this study indicate that variation in BDE-47 accumulation by H. akashiwo corresponds to the change in cellular lipid content induced by different nitrate and phosphate concentrations.
EN
Peroxisome proliferator-activated receptors (PPAR`s) serve as lipid sensors and when activated modify gene expression of proteins highly involved in the regulation of fatty acid metabolism. Recently, the accumulation of lipids in liver was shown to be depended on the excessive protein-mediated transmembrane transport of long chain fatty acids (LCFAs). The aim of the present study was to determine the in vivo effects of PPAR and activation at two levels: 1) on the expression of fatty acid transporters, 2) on the content and fatty acids saturation status of lipids in rats liver. PPAR agonist (WY 14,643) treatment upregulated the liver expression of FAT/CD36 (+20%, p<0.05) and did not significantly affect the content of FABPpm and FATP-1. Accordingly there was a significant increase in the content of phospholipid (+12%, p<0.05), diacylglycerol (+65%, p<0.05) and triacylglycerol (+46%, p<0.05) fractions followed PPAR activation. In contrast, pioglitazone (PPAR agonist) had no effect on the content of fatty acid transporters (FAT/CD36, FABPpm and FATP-1) as well as the content of liver lipid fractions with the exception for triacylglycerols, which have been reduced significantly (-89%, p<0.05). These findings suggest that in vivo PPAR and PPAR activation exert different effects on both the expression of fatty acid transporters and lipid content in rat’s liver.
5
Content available Physical methods of microalgal biomass pretreatment
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EN
The prospect of depletion of natural energy resources on the Earth forces researchers to seek and explore new and alternative energy sources. Biomass is a composite resource that can be used in many ways leading to diversity of products. Therefore, microalgal biomass offers great potential. The main aim of this study is to find the best physical method of microalgal bio- mass pretreatment that guarantees efficient lipid extraction. These studies identifies biochemical composition of microalgal biomass as source for biodisel production. The influence of drying at dif- ferent temperatures and lyophilization was investigated. In addi- tion, wet and untreated biomass was examined. Cell disruption (sonication and microwave) techniques were used to improve lipid extraction from wet biomass. Additionally, two different extrac- tion methods were carried out to select the best method of crude oil extraction. The results of this study show that wet biomass after sonication is the most suitable for extraction. The fatty acid com- position of microalgal biomass includes linoleic acid (C18:2), palmitic acid (C16:0), oleic acid (C18:1), linolenic acid (C18:3), and stearic acid (C18:0), which play a key role in biodiesel production.
EN
This study was aimed at determining lipid content, fatty acid composition and trans isomers content in fat extracted from cereals and cereal bars. Cereals and cereal bars were analyzed by gas chromatography. Analyses showed that they were characterized by a diversified content of fat and composition of particular groups of fatty acids (saturated SFA, monounsaturated MUFA and polyunsaturated PUFA). Only oat flakes turned out to be a good source of PUFA (38.83% of total fatty acids). The remaining products contained more SFA (mean: 45.12% and 47.73% in cereals and 63.31% in cereal bars) than PUFA (mean: 12.24% and 16.73% in cereals and 7.83% in cereal bars). Lipid of all examined products contained trans isomers of C18:1 and C18:2 acids. In lipids of cereals, the total content of these isomers did not exceed 0.5% of the total fatty acids. A higher content of these isomers was found in cereal bars (0.45–3.15%).
PL
Celem badań było określenie zawartości lipidów oraz składu kwasów tłuszczowych i zawartości izomerów trans kwasów tłuszczowych w płatkach i batonikach zbożowych. Płatki i batoniki zbożowe analizowano metodą chromatografii gazowej. W badaniach wykazano, że badane produkty charakteryzowały się zróżnicowaną zawartością lipidów i zróżnicowanym składem poszczególnych grup kwasów tłuszczowych (nasyconych SFA, monoenowych MUFA i polienowych PUFA). Tylko płatki owsiane okazały się dobrym źródłem PUFA (38,83% w ogólnym składzie kwasów tłuszczowych). Pozostałe produkty zawierały więcej SFA (średnio: 45,12% i 47,73% w płatkach i 63,31% w batonikach zbożowych) niż PUFA (średnio: 12,24% i 16,73% płatki i 7,83% batoniki zbożowe). W lipidach wszystkich badanych produktów stwierdzono zawartość izomerów trans kwasu C18:1 i kwasu C18:2. W lipidach płatków całkowita zawartość tych izomerów nie przekracza 0,5% ogólnego składu kwasów tłuszczowych. Wyższe zawartości tych izomerów stwierdzono w batonikach zbożowych (0,45–3,15%).
EN
Variations in lipid, protein and carbohydrate contents of Enteromorpha spp. were examined over a seven-month period from April to October 1993. The samples were collected from seven sampling stations along the Gulf of Gdańsk coast. The lipid content was low and varied slightly from 3.47±1.76% of DW at Puck to 4.36±2.17% of DW at Rewa and Chałupy. The protein content varied from 9.42±4.62% of DW at Puck to 20.60±5.00% of DW at Jurata. At the remaining stations the values vary over a narrow range. The maximum protein contents were recorded at the beginning and end of the growing season. The level of carbohydrate was very high compared to that of lipid and protein and varied from 29.09±6.44% of DW at Osłonino to 39.81±11.15% of DW at Puck. Seasonal carbohydrate changes were noted at all sampling stations, the minimum occurring in spring and autumn and the maximum in summer.
EN
In a long-term study the effects of n-3 and n-6 polyunsaturated fatty acids on certain lipid metabolism parameters were compared. Male Wistar rats were divided into three experimental groups and were given diets with added studied fats. The diets contained 15% of energy from fats (7.5 g/100 g of diet) such as fish oil, sunflower oil and a mixture of butter and lard. At time intervals of 4 weeks, 12 weeks and 12 months the rats were decapitated and lipids were determined in their serum and tissues. In the aorta the axtent of fat deposits was measured plani- metrically. Fish oil was found to reduce most evidently the level of total cholesterol in serum and tissues. Sunflower oil in a lower degree decreased the accumulation of cholesterol in rat organism but increased the concentration of HDL-cholesterol. The atherogenic index in this group was lowest, and the extent of atheromatous lesions was least pronounced. The atherogenic effect of animal fats was confirmed.
PL
W badaniach długoterminowych porównywano działanie wielonienasyconych kwasów tłuszczowych z rodziny n-3 i n-6 na niektóre parametry lipidowe. Szczury, samce rasy Wistar, podzielono na trzy grupy doświadczalne i karmiono dietami z dodatkiem badanych tłuszczów. Diety zawierały 15% energii z tłuszczu (7,5 g/100 g diety) oleju rybnego, oleju słonecznikowego oraz mieszaniny masła i smalcu. W odstępach czasu: 4 tygodnie, 12 tygodni i 12 miesięcy zwierzęta dekapitowano i oznaczano lipidy w surowicy i tkankach. W aorcie oznaczano planimetrycznie rozległość złogów tłuszczowych. Stwierdzono, że olej rybny najsilniej obniża zawartość cholesterolu całkowitego w surowicy i tkankach. Olej słonecznikowy w mniejszym stopniu hamuje gromadzenie się cholesterolu w organizmie szczura, podnosi jednocześnie stężenie cholesterolu w HDL. Wskaźnik aterogenny dla tej grupy zwierząt jest najniższy, powstawanie zmian miażdżycowych najmniejsze. Potwierdzono aterogenne działanie tłuszczów zwierzęcych.
EN
The content of total lipids and lipofuscins was determined in leaves of S. latifolium aerial-aquatic and terrestrial plants at the flowering and fruiting phases. There were no significant differences in the lipid total content between aerial-aquatic and terrestrial plants at the flowering phase. At the fruiting phase, the lipid content was lower in terrestrial plants. Lipofuscins, the so-called senescence pigments, were determined in plants of both ecotypes at the flowering phase, although visible symptoms of senescence were absent. At the fruiting phase, there was an accumulation of lipofuscins in aerial-aquatic and terrestrial plants that may be explained by progressive senescence. The lipofuscin content was higher in terrestrial plants at both phases of ontogenesis. We concluded that earlier and more intensive senescence is typical for terrestrial plants, which are in the conditions of chronic moderate water deficit on the riverside.
PL
W pracy mierzono całkowite stężenie lipidów w liściach roślin S. latifolium dla ekotypu powietrzno-wodnego oraz naziemnego w fazie kwitnienia i owocowania. Nie zaobserwowano istotnych różnic w całkowitym stężeniu lipidów pomiędzy ekotypami w fazie kwitnienia. Natomiast w fazie owocowania zawartość lipidów była wyższa dla ekotypu naziemnego. Badano ponadto zawartość lipofuscyny, nazywanej barwnikiem starzenia, w roślinach obu ekotypów w fazach kwitnienia i owocowania. W fazie kwitnienia nie zaobserwowano symptomów starzenia. U owocujących roślin obu ekotypów obserwowano akumulację lipofuscyny, prawdopodobnie związaną z postępującymi procesami starzenia. W obu fazach stężenie lipofuscyny było wyższe dla ekotypu naziemnego. Na podstawie otrzymanych wyników w pracy postawiono hipotezę, że intensywne starzenie się jest charakterystyczne dla roślin naziemnych, rosnących w warunkach chronicznego umiarkowanego deficytu wody.
EN
In this study, Nusem and Beppo snack seed pumpkin cultivars were used to determine the effects of different plant activators on seed protein, lipid and fatty acids contents. In the context of study, plant activators consist of Crop-set (CR), EM1, ERS, Vitormone-Plus Drip (VIT), Bacillus subtilis (OSU 142), Bacillus megatorium (M3), Azospirillum sp. (SP 245), Spirulina platensis (SIP), Ecocompost (EKO), Camli Botanica liquid organic fertilizer (BOT) and Zincon (ZIN) were used as organic fertilizer. In the experiment, the plant activators were applied to the plants alone or in combination with each other and organic fertilizer. Two separate control groups which were organic and conventional (CONV.) fertilizer have been identified. As a result of the use of different plant activators, the highest protein content was obtained from CONV. application (35.50%), M3+SP 245 (33.09%) and M3 (33.04%); the highest lipid content was observed from SP 245+OG (45.90%), CR (44.48%) and SIP+OG (44.26%) applications. The use of different plant activators effected the fatty acid contents of seeds. Total 11 fatty acids were identified. Among the fatty acids, C16:0 (Palmitic acid), C18:0 (Stearic acid), C18:1 (Oleic acid) and C18:2 (Linoleic acid) were found dominant.
EN
Objectives: Obesity, insulin resistance and dyslipidemia are the most significant risk factors of non-alcoholic fatty liver disease (NAFLD) but the role of adipokines in the pathogenesis of this disease is not clear. Assessment of retinol binding protein (RBP-4) seems to be promising because data from animal and human studies suggest its role in the patomechanism of insulin resistance. Therefore, the aim of the study was to evaluate the serum levels of RBP-4 in children with NAFLD. Methods: Fasting serum level of RBP-4 was determined in 42 obese children with suspected liver disease and 20 lean controls. The degree of liver steatosis was graded in ultrasound according to Saverymuttu. The intrahepatic lipid content was assessed noninvasively in a semiquantitative fashion using 1HMR spectroscopy (1.5-T scanner with PRESS sequence). Results: Fatty liver was confirmed in 30 children by ultrasonography (16 of them had also increased alanine transaminase (ALT) activity). Serum concentrations of RBP-4 were significantly higher in obese children with NAFLD compared to controls. Significant correlations were found between RBP-4 level and ultrasonographic grade of liver steatosis, intrahepatic lipid content (1HMRS) and triglycerides level, while the serum level of RBP-4 was not significantly higher in children with advanced liver steatosis (grade 2-3, n = 11) compared to patients with mild steatosis (grade 1, n = 19). The ability of RBP-4 to differentiate children with advanced liver steatosis from those with mild steatosis was not significant. Conclusion: RBP-4 can be considered as a convenient serum marker of intrahepatic lipid content in obese children.
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