Wyniki wyszukiwania - Biblioteka Nauki

1

Influence of BCR/ABL fusion proteins on the course of Ph leukemias.

100%

Telegeev G. D. , Dubrovska A. N. , Dybkov M. V. , Maliuta S. S.

Acta Biochimica Polonica

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2004

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tom 51

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nr 3

845-849

EN

The hallmark of chronic myeloid leukemia (CML) and a subset of acute lymphoblastic leukemia (ALL) is the presence of the Philadelphia chromosome as a result of the t(9;22) translocation. This gene rearrangement results in the production of a novel oncoprotein, BCR/ABL, a constitutively active tyrosine kinase. There is compelling evidence that the malignant transformation by BCR/ABL is critically dependent on its Abl tyrosine kinase activity. Also the bcr part of the hybrid gene takes part in realization of the malignant phenotype. We supposed that additional mutations accumulate in this region of the BCR/ABL oncogene during the development of the malignant blast crisis in CML patients. In ALL patients having p210 fusion protein the mutations were supposed to be preexisting. Sequencing of PCR product of the BCR/ABL gene (Dbl, PH region) showed that along with single-nucleotide substitutions other mutations, mostly deletions, had occurred. In an ALL patient a deletion of the 5th exon was detected. The size of the deletions varied from 36 to 220 amino acids. For one case of blast crisis of CML changes in the character of actin organization were observed. Taking into account the functional role of these domains in the cell an etiological role of such mutations on the disease phenotype and leukemia progression is plausible.

2

Preliminary crystallographic studies of Y25F mutant of periplasmic Escherichia coli L-asparaginase.

100%

Kozak M. , Jaskólski M. , Röhm K. H.

Acta Biochimica Polonica

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2000

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tom 47

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nr 3

807-814

EN

Periplasmic Escherichia coli L-asparaginase II with Y25F mutation in the active-site cavity has been obtained by recombinant techniques. The protein was crystallized in a new hexagonal form (P6522). Single crystals of this polymorph, suitable for X-ray diffraction, were obtained by vapor diffusion using 2-methyl-2,4-pentanediol as precipitant (pH 4.8). The crystals are characterized by a = 81.0, c = 341.1 Å and diffract to 2.45 Å resolution. The asymmetric unit contains two protein molecules arranged into an AB dimer. The physiologically relevant ABA'B' homotetramer is generated by the action of the crystallographic 2-fold axis along [1, -1, 0]. Kinetic studies show that the loss of the phenolic hydroxyl group at position 25 brought about by the replacement of Y with F strongly impairs kcat without significantly affecting Km.

3

Cytotoxic effect of nitric oxide on human hematological malignant cells.

100%

Tsumori M. , Tanaka J. , Koshimura K. , Kawaguchi M. , Murakami Y. , Kato Y.

Acta Biochimica Polonica

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2002

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tom 49

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nr 1

139-144

EN

We investigated the cytotoxic effect of nitric oxide (NO) on primary culture of human hematological malignant cells. Sodium nitroprusside (SNP), an NO donor, had cytotoxic effects on the cells of some patients with malignant lymphoma (ML), acute myelocytic leukemia (AML) or chronic myelomonocytic leukemia (CMMoL), but not with multiple myeloma. Cultured cells from the ML patient remained sensitive to SNP after the cells became resistant to anti-cancer drugs. In contrast, the cells from the patients with AML and CMMoL became resistant to SNP while anti-cancer drugs remained effective. In samples of the cells of the patients with ML and AML, the number of CD3 positive lymphoma cell was decreased by SNP and the number of CD33 negative cells and normal B lymphocytes (CD19 positive cells) were increased. In the cells of the patient with ML, apoptosis was induced by SNP. SNP had no effect on lymphocytes of healthy volunteers. These results suggest that SNP had an anti-tumor effect on human hematological malignant cells.

4

Major, minor, and trace elements in whole blood of patients with different leukemia patterns

88%

Khuder A. , Bakir M. A. , Solaiman A. , Issa H. , Habil K. , Mohammad A.

Nukleonika

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2012

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tom Vol. 57, No. 3

389-399

EN

The elemental sensitivity method for X-ray fluorescence analysis was applied to determine S, Cl, K, Ca, Fe, Cu, Zn, Br, and Rb in the whole blood of leukemia patients and healthy volunteers. Leukemia samples were classified according to type, growth, and age of participants. Student’s t-test results showed that, the mean concentration of the studied elements was significantly lower in leukemia patients than that in controls. Strong mutual correlations (r greather than 0.50) in the whole blood of leukemia patients were observed between S-Ca, K-Fe, K-Ca, Fe-Zn, K-Zn, K-Rb, Fe-Rb, Zn-Rb, S-Cl, S-K, Ca-Fe, Cl-Ca, and Ca-Rb; whereas, S-K, S-Ca, S-Cl, Cl-K, Cl-Ca, Fe-Zn, Zn-Rb, Fe-Rb, K-Fe, and Zn-Br exhibited strong relationships (r greather than 0.50) in the whole blood of controls, all were significant at p less than 0.05. Significant differences between grouping of studied elements in the control group and all classified leukemia groups, except younger age-group, were obtained using principal component analysis. The study indicated appreciably different patterns of element distribution and mutual relationships in the whole blood of leukemia patients in comparison with controls.

5

In vitro activity of oxazaphosphorines in childhood acute leukemia: Preliminary report.

88%

Styczyński J. , Wysocki M. , Dębski R. , Balwierz W. , Rokicka-Milewska R. , Matysiak M. , Balcerska A. , Kowalczyk J. , Wachowiak J. , Sońta-Jakimczyk D. , Chybicka A.

Acta Biochimica Polonica

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2002

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tom 49

|

nr 1

221-225

EN

Glufosfamide (β-D-glucosyl-ifosfamide mustard) is a new agent for cancer chemotherapy. Its pharmacology is similar to commonly used oxazaphosphorines, but it does not require activation by hepatic cytochrome P-450 and preclinically demonstrates lower nephrotoxicity and myelosuppression than ifosfamide. The aim of the study was a comparison of the drug resistance profiles of glufosfamide and other oxazaphosphorines in childhood acute leukemias. Leukemic cells, taken from children with ALL on diagnosis (n = 41), ALL on relapse (n = 12) and AML on diagnosis (n= 13) were analyzed by means of the MTT assay. The following drugs were tested: glufosfamide (GLU), 4-HOO-ifosfamide (IFO), 4-HOO-cyclophosphamide (CYC) and mafosfamide cyclohexylamine salt (MAF). In the group of initial ALL samples median cytotoxicity values for GLU, IFO, CYC and MAF were 15.5, 33.8, 15.7 and 7.8 μM, respectively. In comparison with initial ALL samples, the relative resistance for GLU and IFO in relapsed ALL samples was 1.9 (p = 0.049) and 1.3 (ns), and in initial AML samples 31 (p < 0.001) and 5 (p = 0.001), respectively. All oxazaphosphorines presented highly significant cross-resistance. Glufosfamide presented high activity against lymphoblasts both on diagnosis and on relapse.

6

Molecular hematology – modern methodology serving patients and research

88%

Witt M.

Nauka

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2009

|

nr 4

PL

The article describes a country-wide attempt to organize a network of laboratories performing molecular diagnostic procedures in a context of clinical hematology. The principles of construction of a commissioned grant in molecular hematology are presented. A major result of this project, the monographic book on molecular hematology is being announced.

7

Correlation between hyperdiploidy 51plus of acute lymphoblastic leukemia and DNA ploidy

88%

Brycz-Witkowska J. , Sikorska-Fic B. , Stanczak H. , Chmarzynska-Mroz E. , Rybczynska J. , Wasiutynski A. , Rokicka-Milewska R. , Wasik M.

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tom 39

|

nr 1

8

Complex translocations with chromosome Philadelphia positive and negative in patients with chronic myelois leukemia [CML]

88%

Stanczak H. , Brycz-Witkowska J. , Chmarzynska-Mroz E. , Malinowska J. , Sachs W. , Wasiutynski A. , Jedrzejczak W. W.

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tom 39

|

nr 1

9

Expression of CD79a antigen in acute lymphoblastic leukaemias in children

88%

Nowicki M. , Miskowiak B. , Konwerska A.

Folia Histochemica et Cytobiologica

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1999

|

tom 37

|

nr 2

10

Detection and molecular identification of Cryptosporidium species among children with malignancies

75%

Ibrahim H. S. , Shehab A. Y. , Allam A. F. , Mohamed M. A. , Farag H. F. , Tolba M. M.

Acta Parasitologica

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2021

|

tom 66

|

nr 2

11

T-cell subsets in the murine thymus during chemically induced leukemogenesis

75%

Seidel H. J. , Kreja L.

Folia Histochemica et Cytobiologica

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1986

|

tom 24

|

nr 1

12

Cytotoxic effects of cladribine and tezacitabine toward HL-60.

75%

Stachnik K. , Grieb P. , Skierski J. S.

Acta Biochimica Polonica

|

2005

|

tom 52

|

nr 2

561-565

EN

The aim of the study was to determine the relation between the cytotoxic and cytostatic effects of tezacitabine and cladribine on a HL-60 cell line and the time of exposure of cells to these drugs. Cell viability and induction of apoptosis were assessed using flow cytometry methods. Apoptosis was confirmed by direct microscopic observation. Growth inhibition was examined by cell counting. After 24 h incubation tezacitabine was equally or less toxic compared to cladribine. However, toxicity of tezacitabine strongly rose after 48 h incubation leading to massive cell death at doses much lower than those of cladribine. Assessment of the effect of increased exposure time on the clinical efficacy of tezacitabine is indicated.

13

Outcomes of Jehovah’s Witnesses with hematological malignancies treated without transfusions — single center experience

75%

Drozd-Sokołowska J. E. , Waszczuk-Gajda A. , Dwilewicz-Trojaczek J. , Walesiak A. , Krzyżanowska M. , Paluszewska M. , Wieczorek J. , Wiktor-Jędrzejczak W. , Drozd-Sokołowska J. E. , Waszczuk-Gajda A. , Dwilewicz-Trojaczek J. , Walesiak A. , Krzyżanowska M. , Paluszewska M. , Wieczorek J. , Wiktor-Jędrzejczak W.

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14

Expression of CD163 and HLA-DR molecules on the monocytes in chronic lymphocytic leukemia patients

75%

Kowalska W.

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nr 1

15

Exposure to electromagnetic fields and risk of leukemia

75%

Pachocki K. A. , Gajewski A. K.

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nr 3

16

A transferrin conjugate of adriamycin-synthesis and potential chemotherapeutic efficacy

75%

Lubgan D. , Jozwiak Z.

Cellular and Molecular Biology Letters

|

2002

|

tom 07

|

nr Suppl.

17

Cytotoxic effects of cladribine and tezacitabine toward HL-60

75%

Stachnik K. , Grieb P. , Skierski J. S.

|

2005

|

tom 52

|

nr 2

EN

The aim of the study was to determine the relation between the cytotoxic and cytostatic effects of tezacitabine and cladribine on a HL-60 cell line and the time of exposure of cells to these drugs. Cell viability and induction of apoptosis were assessed using flow cytometry methods. Apoptosis was confirmed by direct microscopic observation. Growth inhibition was examined by cell counting. After 24 h incubation tezacitabine was equally or less toxic compared to cladribine. However, toxicity of tezacitabine strongly rose after 48 h incubation leading to massive cell death at doses much lower than those of cladribine. Assessment of the effect of increased exposure time on the clinical efficacy of tezacitabine is indicated.

18

In vitro activity of axazaphosphorines in childhood acute leukemia: preliminary report

75%

Styczynski J. , Wysocki M. , Debski R. , Balwierz W. , Rokicka-Milewska R. , Matysiak M. , Balcerska A. , Kowalczyk J. , Wachowiak J. , Sonta-Jakimczyk D. , Chybicka A.

Acta Biochimica Polonica

|

2002

|

tom 49

|

nr 1

EN

Glufosfamide (β-D-glucosyHfosfamide mustard) is a new agent for cancer chemotherapy. Its pharmacology is similar to commonly used oxazaphosphorines, but it does not require activation by hepatic cytochrome P-450 and preclinically demonstrates lower nephrotoxicity and myelosuppression than ifosfamide. The aim of the study was a comparison of the drug resistance profiles of glufosfamide and other oxazaphosphorines in childhood acute leukemias. Leukemic cells, taken from children with ALL on diagnosis (n = 41), ALL on relapse (n = 12) and AML on diagnosis (n = 13) were analyzed by means of the MTT assay. The following drugs were tested: glufosfamide (GLU), 4-HOO-ifosfamide (IFO), 4-HOO-cyclophosphamide (CYC) and mafosfamide cyclohexylamine salt (MAF). In the group of initial ALL samples median cytotoxicity values for GLU, IFO, CYC and MAF were 15.5, 33.8, 15.7 and 7.8,«M, respectively. In comparison with initial ALL samples, the relative resistance for GLU and IFO in relapsed ALL samples was 1.9 (p = 0.049) and 1.3 (ns), and in initial AML samples 31 (p < 0.001) and 5 (p = 0.001), respectively. All oxazaphosphorines presented highly significant cross-resistance. Glufosfamide presented high activity against lymphoblasts both on diagnosis and on relapse.

19

The effects of combined antifolates on inhibition of growth of murine leukemia cells cultured in vitro

75%

Balinska M. , Szablewska I. , Janiszewska D. , Bartuzi K. , Pawelczak K.

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nr 4

EN

The synergistic effect of trimetrexate (TMTX) and sulphonamide derivatives of quinazoline on the cultured 5178Y murine leukemia cells was examined. On exposure to the slightly inhibitory concentrations of TMTX (0.1 nM) in combination with 2-desamino-2-methyl-10-propargyl-5,8-dideaza-pteroyl-sulphoglyc ine (DMPDDSF) (0.02 microM) a synergistic inhibitory effect of the antifolates on cell growth was observed. These two drugs in the same combination caused also synergistic inhibition of de novo synthesis of thymidylate in intact cells as measured by tritium release from [5-(3)H]deoxyuridylate. This was accompanied by a marked reduction in intracellular concentration of 5,10-methylenetetrahydro-pteroyl-polyglutamate (5,10CH2H4PteGlu(n)) (0.2 microM) and dihydropteroyl-polyglutamate (0.12 microM). In these conditions de novo biosynthesis of purine was decreased by 50%. These observations show that growth inhibition by combined antifolates is mediated by intracellular depletion of the substrate of thymidylate synthase -- 5,10CH2H4PteGlu(n). The results obtained strongly suggest that under certain conditions inhibition of thymidylate synthesis by DMPDDSF is intensified by prior application of TMTX -- an inhibitor of dihydrofolate reductase.

20

DNA repair in drug resistance induced by fusion tyrosine kinases

75%

Blasiak J.

Cellular and Molecular Biology Letters

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2002

|

tom 07

|

nr Suppl.