Wyniki wyszukiwania - Biblioteka Nauki

1

In vitro assessment of camphor hydrazone derivatives as an agent against Leishmania amazonensis

100%

da Silva E. T. , de Andrade G. F. , da Silva Araujo A. , das Chagas Almeida A. , Coimbra E. S. , de Souza M. V. N.

Acta Parasitologica

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2020

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tom 65

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nr 1

2

Activity of N-lutidinylarylcarboxamides against Leishmania donovani and L.braziliensis

100%

Le P. P. , Abdala H. , Pagniez F. , Robert J. M. , Le Baut G.

Acta Parasitologica

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1999

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tom 44

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nr 3

EN

Three arylcarboxamides issued from 2-amino- 4,6-dimethylpyridine, benzamide 1, furan-2-carboxamide 2 and 5-bromofuran-2-carboxamide 3 were evaluated against Leishmania promastigotes, at three doses, 0.5, 5, and 50 µM. Although benzamide 1 exerted but a moderate inhibition against cultured extracellular promastigotes at a concentration of 50 µM, furan-2-carboxamides 2 and 3 had potent activity at the same concentration. The IC₅₀ of 2 against L. donovani and L. braziliensis were 29.9 and 28.3 µM and those of 3 were 25.9 and 36.6 µM, respectively. In a BALB/c mice model of visceral leishmaniosis, an intraperitoneal administration of 10 mg/kg of 2, for 5 days, led to a consistent parasite burden reduction in the spleen smears (75.8 ± 5.7%) and in the microdilution spleen cultures (72.1 ± 0.6%). A very significant correlation (r = 0.96) was found between the two methods of spleen parasite burden quantification. These amides also exhibit potent antiinflammatory activity and it was previously stated that they inhibit arachidonic acid production by interfering in the PLA₂ activation. This suggests fact that they could disrupt Leishmania membrane lipid integrity by protein kinase C inhibition.

3

Genome sequencing of Leishmania infantum causing cutaneous leishmaniosis from a Turkish isolate with next-generation sequencing technology

86%

Guldemir D. , Usluca S. , Nalbantoglu A. S.

Acta Parasitologica

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2021

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tom 66

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nr 1

4

Immunosuppression during Leishmania donovani infection: a potential target for the development of therapy

86%

Flora R. , Aghazadeh-Dibavar S. , Bandyopadhyay M. , Dasgupta S.

Annals of Parasitology

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2014

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tom 60

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nr 4

EN

Dysfunction of T-helper 1 mediated immune responses is a hallmark of the progression of visceral leishmaniosis (VL). Several factors such as altered antigen presentation, and abnormalities in MHC/HLA, antigen processing, and T cell receptor recognition regulate the onset of immunosuppression. Recent investigations on VL patients suggest that susceptibility to visceral leishmaniosis is genetically determined and varies between populations in different geographical locations. Emerging evidence also indicates the importance of the role played by myeloid derived suppressor cells in progressive VL. This study provides a mechanistic view of means to target the signaling mechanisms of immunosuppression to determine potential therapeutic interventions.

5

Clinical and pathological aspects of canine cutaneous leishmaniasis: a meta-analysis

86%

Sobotyk Oliveira C. , Ratzlaff F. R. , Potter L. , Romao P. R. T. , de Avila Botton S. , Vogel F. S. F. , Sangioni L. A.

Acta Parasitologica

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2019

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tom 64

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nr 4

6

A case of cutaneous leishmaniasis treated with hyperbaric oxygen therapy

86%

Olszański R. , Siermontowski P. , Juszczak D. , Dąbrowiecki Z. , Pedrycz A.

Polish Hyperbaric Research

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2016

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tom nr 4(57)

39--44

EN

Cutaneous leishmaniasis in Poland is an imported disease mainly occurring in tourists who travelled to tropical countries. Cutaneous symptoms occur as late as between ten and twenty days following the return from the tropics. Lesions connected with cutaneous leishmaniasis were most commonly diagnosed by Polish doctors as furuncle, ecthyma or ulceration and ineffectively treated for several weeks with antibiotics. The paper presents the case of leishmaniasis in a 30-year-old male with an ulceration of the left shank, ineffectively treated with antibiotics over a period of four months. The ulceration was healed completely only after leishmaniasis was diagnosed and following the application of a treatment based on antimony derivatives, followed by hyperbaric oxygenation performed in a hyperbaric chamber.

PL

Leiszmanioza skórna w Polsce jest to chorobą z importu i występuje głównie u turystów, którzy przebywali w krajach tropikalnych. Objawy skórne występują dopiero kilkanaście dni od powrotu z tropiku. Zmiany leiszmaniozy skórnej przez lekarzy w Polsce były najczęściej rozpoznawane jako czyrak, niesztowica lub owrzodzenie i nieskutecznie były leczone przez kilka tygodni antybiotykami. Przedstawiono przypadek leiszmaniozy u 30. letniego mężczyzny z owrzodzeniem podudzia lewego, którego nieskutecznie leczono antybiotykami przez cztery miesiące. Dopiero po rozpoznaniu leiszmaniozy i zastosowaniu leczenia pochodnymi antymonu, a następnie hiperbarią tlenową w komorze hiperbarycznej, nastąpiło całkowite wyleczenie owrzodzenia.

7

A laboratory strain of Leishmania major: protective efects on experimental leishmaniasis

86%

Namavari M. , Namazi F. , Asadi-Manesh R. , Hosseini M. H. , Nazifi S. , Asadpour M.

Acta Parasitologica

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2019

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tom 64

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nr 3

8

Overcoming the challenge; In Vivo efficacy of miltefosine for chronic cutaneous leishmaniasis

86%

Tunali V. , Harman M. , Cavus I. , Gunduz C. , Ozbilgin A. , Turgay N.

Acta Parasitologica

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2021

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tom 66

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nr 2

9

Characterization of transplasma membrane electron transport chain in wild and drug-resistant Leishmania donovani promastigote and amastigote

86%

Debnath D. , Hossain M. A. , Das M. , Kabir A. , Ibrahim M. , Amin M. N. , Chowdhury A. , Pervin R.

Acta Parasitologica

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2019

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tom 64

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nr 4

10

A new automatized fluorometric assay for anti-Leishmania drug screening

86%

Le P. P. , Pagniez F. , Abdala H.

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nr 1

EN

The therapeutic armentarium against leishmaniasis is very restricted, using mainly up to now drugs showing several side effects and resistance. Consequently, there is a real need to find new compounds as alternatives for the treatment of leishmaniasis. However, most of the classic antileishmanial primary screening assays are not suitable for measuring the cytotoxicity of large number of drug candidates because of the manipulations required. We have established a new assay that incorporate a fluorometric growth indicator based on the detection of a metabolic activity in culture medium after the chemical reduction of alamar Blue® by cells. This antileishmanial test was evaluated on amphotericin B, meglumine antimoniate, allopurinol, ketoconazole and edelfosine. The results reported show the advantages oj this fluorochrome on the tetrazolium salt 3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyltetrazolium bromide (MTT) for Leishmania cytotoxicity measurement. Moreover, this study is the first report of the use of alamar Blue® fluorogenic assay for activity assessment of potential antileishmanial drugs against promastigotes.

11

GK1 improves the immune response induced by dendritic cells of BALB/c mice infected with Leishmania mexicana promastigotes

72%

Gutierrez-Kobeh L. , Wilkins-Rodriguez A. A.

Acta Parasitologica

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2020

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tom 65

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nr 1

12

A comprehensive review of cutaneous leishmaniasis in Sri Lanka and identification of existing knowledge gaps

72%

Amarasinghe A. , Wickramasinghe S.

Acta Parasitologica

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2020

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tom 65

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nr 2

13

Canine leishmaniasis prevalence in the Slovenian dog population

72%

Kotnik T. , Moreno J. , Soba B. , Krt B. , Skvarc M. , Rataj A. V. , Bajc M. G. , Verbic U. R.

Journal of Veterinary Research

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2021

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tom 65

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nr 2

14

Recombinant C-reactive protein: a potential candidate for the treatment of cutaneous leishmaniasis of BALB/c mice caused by Leishmania major

72%

Zahedi S. N. , Hejazi S. H. , Boshtam M. , Amini F. , Fazeli H. , Sarmadi M. , Rahimi M. , Khanahmad H.

Acta Parasitologica

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2021

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tom 66

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nr 1

15

The role of toll-like receptor agonists in the immunotherapy of leishmaniosis. An update and proposal for a new form of anti-leishmanial therapy

72%

Dasgupta S. , Aghazadeh-Dibavar S. , Bandyopadyay M.

Annals of Parasitology

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2014

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tom 60

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nr 2

EN

The use of toll-like receptor agonists in immunotherapy is a new approach in the prevention of immunosuppression during fatal Leishmania parasite infection. The objective of such immunotherapy is to activate specific cell-mediated immune responses, macrophage activation and antigen-responsive inflammation, to kill intracellular amastigotes. Toll-like receptor agonist-based treatment in immunocompetent hosts can be effective either by selective use of the agonists alone or in combination with the anti-leishmanial drug stibanate. Recent investigations suggest that toll-like receptor signal pathways constitute a possible new mode of anti-leishmanial treatment. This article describes the prospect of toll-like receptor – mediated signal pathways in the immunotherapy of cutaneous and visceral leishmaniosis, as well as post kala-azar dermal leishmaniosis (PKADL), a skin-sequel of visceral infection. Suitable synthetic agonists need to be developed for toll-like receptors to overcome immunosuppression.

16

Seroprevalence, clinical, and pathological characteristics of canine leishmaniasis in a central region of Colombia

72%

Picon V. , Almario G. , Rodriguez V. , Garcia N. V.

Journal of Veterinary Research

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2020

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tom 64

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nr 1

17

Comparative assessment of induced immune responses following intramuscular immunization with fusion and cocktail of LeIF, LACK and TSA genes against cutaneous leishmaniasis in BALB/c mice

72%

Maspi N. , Ghaffarifar F. , Sharifi Z. , Dalimi A. , Dayer M. S.

Archivum Immunologiae et Therapiae Experimentalis

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2018

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tom 66

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nr 1

18

Usefulness of PCR method for detection of Leishmania in Poland

72%

Myjak P. , Szulta J. , de Almeida M. E. , da Silva A. J. , Steurer F. , Lass A. , Pietkiewicz H. , Nahorski W. L. , Goljan J. , Knap J. , Szostakowska B.

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nr 3

219-222

EN

Leishmania parasites are the etiological agents of leishmaniosis, with severe course and often fatal prognosis, and the global number of cases has increased in recent decades. The gold standards for the diagnosis of leishmaniosis are microscopic examinations and culture in vitro of the different clinical specimens. The sensitivity of these methods is insufficient. Recent development in specific and sensitive molecular methods (PCR) allows for detection as well as identification of the parasite species (subspecies). The aim of the study was to estimate the usefulness of molecular methods (PCR) for detection of Leishmania species and consequently for the implementation of such methods in routine diagnostics of leishmaniosis in Polish patients returning from endemic areas of the disease. In our investigations we used 54 known Leishmania positive DNA templates (from culture and clinical specimens) received from the CDC (Atlanta, GA, USA). Moreover, 25 samples of bone marrow, blood or other tissues obtained from 18 Polish individuals suspected of leishmaniosis were also examined. In PCR we used two pairs of primers specific to the conserved region of Leishmania kinetoplast DNA (kDNA) minicircle (13A/13B and F/R). Using these primers we obtained amplicons in all DNA templates from the CDC and in three Polish patients suspected for Leishmania infection. In one sample from among these cases we also obtained positive results with DNA isolated from a blood specimen which was previously negative in microscopic examinations.

19

Cutaneous leishmaniasis mimicking granulomatous cheilitis and treated successfully with oral fluconazole in a boy

72%

Serarslan G. , Aksakal M.

Annals of Parasitology

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2015

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tom 61

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nr 3

EN

Cutaneous leishmaniasis is a protozoan infectious disease and widespread in Mediterranean basin including Turkey. Lesions usually start with erythematous papules, gradually enlarges and afterwards it ulcerates. We present a 12-year-old boy with diffuse persistent lip swelling mimicking granulomatous cheilitis. Systemic glucantime was started. However, severe hypotension and bradycardia was developed after injection. Oral fluconazole was started and the lesion resolved completely. Cutaneous leishmaniasis can have varied clinical manifestations and should be suspected especially in endemic areas. Oral fluconazole seems to be safe and effective treatment modality in paediatric cases.

20

Oligopeptidase B-2 from Leishmania amazonensis with an unusual C-terminal extension

72%

De M. G. H. L. , De Carvalho R. S. N. , De Oliveira Gomes D. C. , Rossi-Bergmann B. , De-Simone S. G.

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nr 2

EN

The oligopeptidase B serine protease is an important virulence factor and therapeutic target in Trypanosoma infections. Recently, the Leishmania major Genome Project identified a new oligopeptidase B that was denominated oligopeptidase B-like, herein named oligopeptidase B-2. In this study, a complete open reading frame of oligopeptidase B-2 from Leishmania amazonensis (PH8 strain) was amplified by PCR using primers designed for the oligopeptidase B-2 gene of L. major. The 2,715 bp fragment coded for a protein of 905 amino acids with a predicted molecular mass of 103,918.9 Da and theoretical pI of 5.82. The encoded protein displayed ∼96% identity with L. major and ∼75% identity with Trypanosoma cruzi and T. brucei oligopeptidases B-2, and ∼21% identity with Escherichia coli and L. amazonensis classical oligopeptidase B. An unusual C-terminal extension was found in relation to the classical trypanosomatid oligopeptidase B. By sequence alignment, we determined a catalytic triad (Ser 629, Asp 717 and His 758), S1 subsite (Glu 674 and Glu 676) and suggest a difference in the S2 subsite of L. amazonensis oligopeptidase B-2. We also found that the oligopeptidase B-2 gene is expressed in all cycle stages of L. amazonensis. A phylogenetic analysis indicated that oligopeptidase B-2 is a new member of oligopeptidase B.