Wyniki wyszukiwania - Biblioteka Nauki

1

Simplified solution of lactate dehydrogenase catalysed reaction

100%

Kuczek M.

Cellular and Molecular Biology Letters

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1998

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tom 03

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nr 2

EN

The lactate dehydrogenase catalysed reaction shows lag phase. This lag phase is easy for explanation if the consecution of first and second order equilibrium reactions were assumed for calculation of pyruvate trace concentration. The same explanation was accepted for calculation of significant pyruvate concentration. For calculation significant pyruvate concentration the consecution of simple Michaelis - Menten type and second order reactions was assumed. The exact solution of reaction rate equations system for this complex reaction was counted.

2

Changes in creatine kinase and lactate dehydrogenase activity, and in myoglobin concentration during strength efforts

88%

Slowinska M. , Stefaniak T. , Sobiech K. A.

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tom 07

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nr 6

3

Group II intron-mediated deletion of lactate dehydrogenase gene in an isolated 1,3-propanediol producer Hafnia alvei AD27

75%

Celińska E. , Drożdżyńska A. , Wita A. , Juzwa W. , Białas W. , Czaczyk K. , Grajek W.

Acta Biochimica Polonica

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2017

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tom 64

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nr 1

123-133

EN

Our previous studies showed that glycerol fermentation by Hafnia alvei AD27 strain was accompanied by formation of high quantities of lactate. The ultimate aim of this work was the elimination of excessive lactate production in the 1,3-propanediol producer cultures. Group II intron-mediated deletion of ldh (lactate dehydrogenase) gene in an environmental isolate of H. alvei AD27 strain was conducted. The effect of the Δldh genotype in H. alvei AD27 strain varied depending on the culture medium applied. Under lower initial glycerol concentration (20 gL-1), lactate and 1,3-propanediol production was fully abolished, and the main carbon flux was directed to ethanol synthesis. On the other hand, at higher initial glycerol concentrations (40 gL-1), 1,3-propanediol and lactate production was recovered in the recombinant strain. The final titers of 1,3-propanediol and ethanol were similar for the recombinant and the WT strains, while the Δldh genotype displayed significantly decreased lactate titer. The by-products profile was altered upon ldh gene deletion, while glycerol utilization and biomass accumulation remained unaltered. As indicated by flow-cytometry analyses, the internal pH was not different for the WT and the recombinant Δldh strains over the culture duration, however, the WT strain was characterized by higher redox potential.

4

Cystathionine gamma-lyase [EC 4.4.1.1]: an enzymatic assay of alpha-ketobutyrate using lactate dehydrogenase

75%

Czubak J. , Wrobel M. , Jurkowska H.

Acta Biologica Cracoviensia. Series Zoologia

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2002

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tom 44

5

Regulating the influence of NAD, ATP, and Mgplus2 on the interaction of lactate dehydrogenase [LDH] from Lactobacillus leischmannii with cardiolipin bilayers

75%

Terlecki G. , Rogozik K. , Czapinska E. , Gutowicz J.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr Suppl.

6

Further evidence for the importance of lipid bilayers in the interaction between lactate dehydrogenase and phosphatidylserine

75%

Terlecki G. , Gutowicz J.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr 3

EN

Lactate dehydrogenase (LDH) is one of the glycolytic enzymes, which have been proved to have the capability to reverse non-specific adsorption on cellular membranous structures in vitro, as well as on the structural proteins of the contractile system of muscle cells. It has been suggested that this binding may play a physiological role, as it alters the enzyme’s kinetic properties. Our previous studies on this enzyme showed that its interaction with some anionic phospholipids reveals similar characteristics and similar effect on the activity of the enzyme to those wich had been observed for the interaction with membranous structures. Disruption of the lipid bilayers by nonionic detergent (Tween 20) restored the enzyme activity inhibited by the presence of phosphatidylserine (PS) liposomes. In this study, we used the measurement of enzyme tryptophanyl fluorescence spectra to monitor the interaction and possible changes in the enzyme conformation. The investigation provided further evidence of the importance of the bilayer structure in this interaction. Similarly to the effect on the activity of the enzyme, the addition of Tween 20 diminishes the quenching of the LDH tryptophanyl ﬂuorescence, and finally completely restores the fluorescence.

7

Hypoxic induction of alcohol and lactate dehydrogenases in lupine seedlings

75%

Garnczarska M.

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tom 24

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nr 3

8

Excitatory neurosteroids attenuate apoptotic and excitotoxic cell death in primary cortical neurons

63%

Leskiewicz M. , Jantas D. , Budziszewska B. , Lason W.

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tom 59

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nr 3

457-475

EN

Some neurosteroids show neuroprotective action in in vitro and in vivo studies, but their interaction with apoptotic/necrotic processes has been only partially unraveled. The aim of the present study was to examine the effect of dehydroepiandrosterone (DHEA), dehydroepiandrosterone sulfate (DHEAS), pregnenolone (PGL) and allopregnanolone (Allo) on staurosporine-, glutamate-, and NMDA-induced damage in primary cortical neuronal culture. DHEA, DHEAS and PGL (0.1 and 1 µM) inhibited the staurosporine-evoked LDH release and decreased the number of apoptotic cells as shown by Hoechst`s staining, whereas Allo was without effect. The neurosteroids affected neither the staurosporine-evoked changes in caspase-3 activity nor the decrease in mitochondrial membrane potential. It was also shown that protective effects of DHEA, DHEAS and PGL against staurosporine-induced LDH release were attenuated by extracellular signal-regulated kinase (ERK) - mitogen-activated protein kinase (MAPK) inhibitor – PD 98059 (5 µM) but not by phosphatidylinositol-3-kinase (PI3-K) inhibitors such as LY 294002 (1 µM) or wortmannin (10 nM). The involvement of ERK2-MAPK in protective effects of neurosteroids was confirmed by Western blot study. Further study demonstrated that glutamate-induced cell damage was attenuated by DHEA, DHEAS, and PGL, but not by Allo. None of the steroids influenced NMDA-induced LDH release. The results of the present in vitro studies suggest that excitatory neurosteroids DHEA, DHEAS and PGL at physiological concentrations participate in the inhibition of cortical neuronal degeneration elicited by staurosporine and glutamate, whereas the most potent positive modulator of GABAA receptor - Allo - has no effect. Moreover, neurosteroids appear to attenuate the staurosporine-induced cell damage in a caspase-3 independent way and their neuroprotective mechanism of action involves the increase in ERK-MAPK phosphorylation.

9

The patterns of MHC association in aplastic and non-aplastic paroxysmal nocturnal hemoglobinuria

63%

Nowak J. , Mika-Witkowska R. , Mendek-Czajkowska E. , Rogatko-Koros M. , Graczyk-Pol E. , Pyl H. , Klimczak A. , Wojcik M. , Prochorec-Sobieszek M. , Maryniak R. , Zupanska B.

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nr 3

10

Comparison of prostaglandin and cimetidine in protection of isolated gastric glands against indomethacin injury

63%

Brzozowski T. , Tarnawski A. , Hollander D. , Sekhon S. , Krause W. J. , Gergely H.

Journal of Physiology and Pharmacology. Supplement

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2005

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tom 56

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nr 5

75-88

EN

Prostaglandins can protect the in vivo gastric mucosa against necrosis produced by a variety noxious agents. Cimetidine has also been shown to have protective properties in humans and in some models of experimental injury. Whether prostaglandins or cimetidine may protect gastric mucosal cells directly in the absence of systemic factors remains controversial. In the present study, the potential protective actions of prostaglandin and cimetidine against indomethacin injury were assessed in isolated rat gastric glands. Gastric glands were pre-incubated in oxygenated medium with either placebo, 16,16 dimethyl prostaglandin E2 (dm PGE2) or cimetidine and incubated at 37°C in medium containing 0.5 mg/ml of indomethacin for 2, 4 and 6 hrs. Cell injury and protection was assessed by the Fast Green exclusion test (viability test), leakage of lactate dehydrogenase (LDH) into the medium, and by scanning and transmission electron microscopy. In addition, the generation of PGE2 by the gland cells was determined using RIA assay. Indomethacin by itself significantly reduced the viability of gastric glands, increased LDH release into the medium and produced prominent ultrastructural damage. In contrast to cimetidine, co-incubation of gastric glands with dm PGE2 added to indomethacin, significantly reduced indomethacin-induced injury, increased the number of viable cells, reduced LDH leakage and diminished the extent of ultrastructural damage. The dose of indomethacin (5 µg/ml) which significantly inhibited the generation of PGE2 (up to 90% inhibition) had no effect on cell viability nor LDH release. We conclude that 1) exogenous PGE2 exerts a potent protective activity in vitro which is independent on neural, vascular and hormonal factors; 2) inhibition of endogenous PGs may not the primary mechanism in the deleterious action of indomethacin against damage to gastric glandular cells and 3) indomethacin can exert a direct cytotoxic effect on the mucosal cells in gastric glands.

11

The role of lipid phase structure in the interaction of lactate dehydrogenase with phosphatidylserine. Activity studies

63%

Terlecki G. , Czapinska E. , Gutowicz J.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr 3

EN

Lactate dehydrogenase is one of the enzymes of the glycolytic path. It has been shown to be able to bind in vitro to cellular membranes. The presence of anionic phospholipids induces changes in the catalytic properties of the enzyme similar to those found when the enzyme is bound to natural membranes. In this study, a nonionic detergent (Tween 20), at concentrations not affecting the catalytic activity of LDH, was used to study the role of the lipid supra-molecular structure in the interaction between pig skeletal muscle lactate dehydrogenase and phosphatidylserine. Tween 20 changes the equilibrium of concentrations between the lipid supra-molecular forms. The detergent at the used concentration values did not alter the activity of the enzyme when it was used on its own, but did diminish the level of inhibition induced by the studied phospholipid. The obtained results showed that the interaction is reversible and that the bilayer structure of the lipid is essential for the inhibition.

12

Serum LDH isoenzyme activity in dairy and beef cows

63%

Sobiech P. , Kuleta Z. , Jalynski M.

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2002

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tom 01

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nr 2

13

The effect of some epsilon-aminocaproic acid derivatives on platelet responses

63%

Bruzgo I. , Tomasiak M. , Stelmach H. , Midura-Nowaczek K.

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nr 1

EN

ε-Aminocaproic acid (EACA) is a synthetic low molecular drug with antifibrinolytic activity. However, treatment with this drug can be incidentally associated with an increased thrombotic tendency. The aim of the present work was to test synthetic EACA derivatives for their antiplatelet activities. We investigated the effect of three EACA derivatives with antifibrinolytic activity: I. ε-aminocaproyl-L-leucine hydrochloride (HCl*H-EACA-L-Leu-OH), II. ε-aminocaproyl-L-(S-benzyl)-cysteine hydrochloride (HCl*H-EACA-L-Cys(S-Bzl)-OH) and III. ε-aminocaproyl-L-norleucine (H-EACA-L-Nle-OH) on platelet responses (aggregation and adhesion) and on their integrity. It was found that: 1. as judged by LDH release test, none of the tested compounds, up to 20 mM, was toxic to platelets, 2. in comparison with EACA, all the synthetic derivatives inhibited much stronger the ADP- and collagen-induced aggregation of platelets suspended in plasma (platelet rich plasma) and aggregation of these cells in whole blood, 3. EACA and its derivatives exerted a similar inhibitory effect on the thrombin-induced adhesion of platelets to fibrinogen-coated surfaces. Since platelet activation and blood coagulation are tightly associated processes, the antiplatelet properties of EACA derivatives are expected to indicate reduced throm- botic properties of these derivatives compared to EACA.

14

Alterations of LDH and its isoenzyme activity in blood plasma of ewe lambs after exposure to chlorine in drinking water

63%

Sutiakova I. , Sutiak V. , Krajnicakova M. , Bekeova E.

Bulletin of the Veterinary Institute in Puławy

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2004

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tom 48

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nr 1

15

The influence of heavy metal ions on the viability and metabolic enzyme activity of the marbled crayfish Procambarus virginalis (Lyko, 2017)

51%

Marenkov O. , Prychepa M. , Kovalchuk J.

International Letters of Natural Sciences

|

2018

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tom 70

EN

The article shows the results of studies on the influence of heavy metal ions (manganese, nickel, lead) on the viability and metabolic enzyme activity of marbled crayfish Procambarus virginalis (Lyko, 2017) (Decapoda). Due to the fact that marbled crayfish got into the reservoirs of the Dnipropetrovsk region in 2015, it was necessary to study the possibilities of its adaptation to environmental factors of reservoirs for further prediction of its distribution or even acclimatization under conditions of toxicological contamination of the ponds of the steppe Prydniprovya. In the experiment with marbled crayfish, chronic effects of various concentrations of heavy metal ions on the physiological state and enzyme activity were investigated. The obtained results showed that among the investigated heavy metals nickel ions influenced the weight indexes and mortality of crustaceans the most negatively. According to the results of the research, significant changes were noted in the individual biochemical parameters of marbled crayfish under the influence of manganese, lead and nickel ions. The most significant changes in the activity of lactate dehydrogenase were detected in muscle tissues affected by manganese and nickel ions. A significant decrease in the activity of succinate dehydrogenase in muscle of marbled crayfish was determined after the action of heavy metal ions. Investigation of changes in the activity of alkaline phosphatase under the influence of the ions of manganese, lead and nickel has its own characteristics, which indicates certain violations in the tissues of cell membranes. Changes in the activity of enzymes were also reflected in the overall protein content. In conclusion, changes in these parameters may indicate a rapid biochemical response of crustaceans to the toxic effects of heavy metals.

16

Double-edged sword behaviour of gallic acid and its interaction with peroxidases in human microvascular endothelial cell culture (HMEC-1). Antioxidant and pro-oxidant effects

51%

Serrano J. , Cipak A. , Boada J. , Gonzalo H. , Cacabelos D. , Cassanye A. , Pamplona R. , Zarkovic N. , Portero-Otin M.

Acta Biochimica Polonica

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2010

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tom 57

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nr 2

EN

A previous report from our group had shown in vitro a direct interaction between peroxidases and dietary antioxidants at physiological concentrations, where in the absence of H2O2, the antioxidants could serve as oxidizing substrates for the peroxidases. However, the physiological relevance of those findings had not been evaluated. The main objective of this study was to determine whether the oxidizing products produced in the interaction between peroxidase and gallic acid at a physiological concentration of 1 μM may promote cell death or survival in a human microvascular endothelial cell line (HMEC-1). Our findings suggested that gallic acid may show a double-edged sword behaviour, since in the absence of H2O2 it may have a pro-oxidant effect which may promote cell injury (evidenced by LDH, Crystal Violet and calcein AM viability/citotoxicity assays), while in the presence of H2O2, gallic acid may act as an antioxidant inhibiting oxidative species produced in the peroxidase cycle of peroxidases. These observations were confirmed with several oxidative stress biomarkers and the evaluation of the activation of cell survival pathways like AKT and MAPK/ERK.

17

Synergistic effects of amyloid peptides and lead on human neuroblastoma cells

51%

Suresh C. , Johnson J. , Mohan R. , Chetty C. S.

Cellular and Molecular Biology Letters

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2012

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tom 17

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nr 3

EN

Aggregated amyloid peptides (AP), major components of senile plaques, have been considered to play a very important and crucial role in the development and neuro-pathogenesis of Alzheimer’s disease (AD). In the present in vitro, study the synergistic effects of Pb2+, a heavy metal, and AP on the human neuroblastoma SH-SY5Y cells were investigated. The cells treated with Pb2+ (0.01–10 μM) alone exhibited a significant decrease in viability and IC50 was 5 μM. A similar decrease in viability was also observed when the cells were exposed to AP, Aβ1–40 (20–120 μM) and Aβ25-35 (2.5–15 μM) for 48 hrs. The IC50 values were 60 μM and 7.5 μM for Aβ1–40 and Aβ25–35 respectively. To assess the synergistic effects the cells were exposed to IC50 of both AP and Pb2+, which resulted in further reduction of the viability. The study was extended to determine the lactate dehydrogenase (LDH) release to assess the cytotoxic effects, 8-isoprostane for extent of oxidative damage, COX 1 and 2 for inflammation related changes, p53 protein for DNA damage and protein kinases A and C for signal transduction. The data suggest that the toxic effects of AP were most potent in the presence of Pb2+, resulting in an aggravated clinical pathological condition. This could be attributed to the oxidative stress, inflammation neuronal apoptosis and an alteration in the activities of the signaling enzymes.

18

The effects of cadmium on the absorption of thiamine, calcium, magnesium and phosphates and on the activity of lactate dehydrogenase (LDH) in the intestines of rats

51%

Dolezych S. , Dolezych B. , Szmatloch A. , Mekail A. , Jethon Z. , Bytomska S.

Acta Biologica Silesiana

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1990

|

tom 15

19

Wplyw fluoru na aktywnosc dehydrogenazy mleczanowej w hepatocytach szczura

51%

Dabrowska E.

Bromatologia i Chemia Toksykologiczna

|

2001

|

tom 34

|

nr 4

311-314

PL

Badano odczyn histochemiczny na aktywność dehydrogenazy mleczanowej [LDH 1.1.1.27] w wątrobach szczurów poddanych działaniu fluorku sodu (NaF). W wyniku przeprowadzonych badań stwierdzono zmiany w aktywności LDH w wątrobach szczurów po podaniu NaF z wodą do picia.

EN

The aim of the study was to evaluate the effect of fluorine on the activity of lactate dehydrogenase (LDH) in rat hepatocytes. Wistar rats (n = 90) were used in the experiment. The animals were divided into 3 groups; the control group was drinking tap water with trace amounts of fluoride, while two other groups received fluoride at 10.6 mg NaF/dm3 and 32.0 mg NaF/dm3, respectively. The rats were exposed to fluoride for 4 months since the intrauterine life to maturity. It has been found that long-term dietary exposure to sodium fluoride leads to changed LDH activity in the rat liver and that the applied fluoride dose affects reversibility of the changes in lactate dehydrogenase activity.

20

Muscle biochemistry in rainbow trout Oncorhynchus mykiss following Yersinia ruckeri vaccination

51%

Tkachenko H. , Grudniewska J. , Pekala A.

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tom 20

EN

Vaccination of rainbow trout against Yersiniosis confers a high degree of protection to the fish (Raida et al. 2011). On the other hand, vaccination could alter metabolitic reactions in organisms. Therefore, exploring the effects of vaccination against Y. ruckeri on health condition of trout in general, and oxidative stress biomarkers and biochemical alterations in different tissues specifically, would be valuable. This prompted us to investigate theeffects of vaccination against Y. ruckeri on muscle function, and the oxidative mechanism underlying those effects, by detecting relevant lipid peroxidation (2-thiobarbituric acid reactive substances, TBARS) and protein oxidation biomarkers (aldehydic derivatives and ketonic derivatives) as well as biochemical alterations (aminotransferases and lactate dehydrogenase activity, lactate and pyruvate levels) in rainbow trout Oncorhynchus mykiss following Y. ruckeri vaccination at first month after oral immunization. Concentrated vaccine with inactivated by formalin Y. ruckeri strains was enclosed by fish feed, and was administered three times every other day. Rainbow trout from each group were euthanized 30 days after the immunization, and then muscle tissue were sampled for analysis. The TBARS level in the muscle tissue of vaccinated group was at same level compared to unhandled group. The ketonic derivatives of oxidatively modified proteins in the trout following Y. ruckeri vaccination at first month after immunization were significantly increased compared to the level in the controls, while the aldehydic derivatives of oxidatively modified proteins were non-significantly increased. Pyruvate level was increased by 47% (p = 0.013) in vaccinated trout compared to values of untreated fish. Lactate level, aminotransferases and lactate dehydrogenase activities were nonsignificantly altered in vaccinated trout. Our results suggest that vaccination could promote the activation of the gluconeogenic substrate-providing enzymes, as well as substrates for aerobic metabolism that might in turn contribute to increase of oxidatively modified proteins. The oxidative stress biomarkers, i.e. content of oxidative protein damage, as well as biochemical enzymes and substrates were sensitive to vaccination of trout against Y. ruckeri and may potentially be used as biomarkers in evaluating vaccine toxicity in rainbow trout. From a practical point of view, the results may be useful in relation to studies of infections and the development, administration and uptake of new vaccines applicable for large amounts of fish.

PL

Ciągłe ulepszanie i wprowadzanie nowych, cechujących się wysoką skutecznością, metod ochrony zdrowia ryb to podstawowe czynniki, decydujące o ekonomicznych efektach oraz postępie w chowie i hodowli ryb. Duże znaczenie mają metody ochrony zdrowia tarlaków, których kondycyja oraz stan zdrowia mają znaczący wpływ na uzyskanie od nich zdrowego potomstwa. Celem badań była analiza mechanizmów kształtowania się reakcji odpornościowych ryb przez ocenę stężenia markerów stresu oksydacyjnego (produkty reagujące z kwasem 2-tiobarbiturowym, aldehydowe i ketonowe pochodne oksydacyjnej modyfikacji białek) i przemian metabolicznych (aktywność aminotransferaz alaninowej i asparaginianowej, dehydrogenazy mleczanowej, stężenie mleczanu i pirogronianu) w tkance mięśniowej młodocianych osobników pstrąga tęczowego, które to mechanizmy decydują o efektywności stosowania szczepionki przeciwko Yersinia ruckeri w pierwszym miesiącu po immunizacji doustnej. Ryby zostały podzielone na dwie grupy doświadczalne. Pierwszą z nich karmiono paszą bez dodatków i konserwantów (firma „Bestfeed”), drugą paszą z inaktywowanymi antygenami Y. ruckeri podawaną 3 razy co drugi dzień, a w pozostałe dni podawano paszę kontrolną. Po pierwszym miesiącu od zakończenia immunizacji szczepionką przeciwko Y. ruckeri z grup kontrolnej i doświadczalnej pobrano ryby do badań. Szczepionka zawierała trzy szczepy Y. ruckeri pochodzące z pstrągów tęczowych hodowanych na różnych farmach, gdzie ryby wykazywały kliniczne objawy jersiniozy. Wyizolowane bakterie należały do serotypu O1. Po określeniu opcjonalnych warunków szczepień (dawki antygenów, czasu ekspozycji ryb), młodociane osobniki pstrąga tęczowego karmiono paszą z inaktywowanymi antygenami Y. ruckeri. Zaobserwowano statystycznie istotne zwiększenie poziomu ketonowych pochodnych oksydacyjnie zmodyfikowanych białek między średnimi w grupach immunizowanej i kontrolnej po upływie pierwszego miesiąca po szczepieniu. Aktywacja proteolitycznej degradacji zmodyfikowanych reszt aminokwasowych może być jedną z przyczyn aktywacji metabolizmu pirogronianu w wyniku adaptacji do immunizacji. Korelacyjne zależności między stężeniem markerów stresu oksydacyjnego oraz metabolitami w tkance mięśniowej pstrąga tęczowego immunizowanego szczepionką przeciwko Y. ruckeri w pierwszym miesiącu po szczepieniu potwierdzają ważną rolę metabolitów i enzymów przemian energetycznych w przebiegu stresu oksydacyjnego spowodowanego immunizacją pstrąga tęczowego przeciwko Y. ruckeri. Wyniki badań wykazały, że szczepionka skierowana przeciwko jersiniozie nie wywiera negatywnego wpływu na kondycję i zdrowie ryb. W układach doświadczalnych nie zarejestrowano jakichkolwiek zmian o charakterze klinicznym u osobników poddanych wakcynacji, co jest dowodem na to, że szczepionka nie jest toksyczna.