Przebadano wpływ jonów Zn2+ i egzogennej melaniny na kumulację i szybkość wydalania jonów Mn2+ u myszy Balb c ( nie zawierających endogennej melaniny ) i C-57 Black (zawierających w sierści melaninę).
EN
The effect of intraperitoneally administered zinc ions and exogenous melanin on the rate of manganese ions accumulation and elimination in Balb c and C-57 Black mice was studied. The content of metal ions in the mice was measured radiochemical using 54MMn and 65Zn isotopes added in trace amounts to the ion solutions just before injection. The analysis of Mn2+ two-phase elimination in mice demonstrated that zinc ions accelerated the elimination of the manganese ions in Balb c and C-57 Black mice. At the same time, an increase of Mn2+ elimination half-times and Mn2+ accumulation in the mice in the presence of exogenous melanin was observed.
Przeprowadzone badania dotyczyły zmian w syntezie DNA, RNA i białek w komórkach jelita cienkiego myszy w warunkach długotrwałej, trzymiesięcznej intoksykacji kadmem i selenem oraz ustalenia, czy selen modyfikuje zmiany wywołane działaniem kadmu.
EN
Changes of DNA, RNA and protein synthesis in small intestine cells of white Balb mice exposed for 3 months to cadmium and selenium were studied. The animals received per os cadmium (50 ppm), selenium (0,4 mg/kg b.w.), or both elements in doses as specified. The control and test animals were injected intraperitoneally a single dose (1 µСі/g b.w.) of the radioactive precursor: 63H-timidine (DNA precursor), 5,63H-uridine (RNA precursor) and L-3H-alanine (protein precursor). Cadmium caused a decrease in the intensity of DNA synthesis, and a slight increase in the intensity of RNA and protein synthesis in the small intestine cells. Three-month exposure to selenium at the threshold dose reduced the intensity of nucleic acid and protein synthesis in the cells. In that case, selenium showed a stronger toxic effect on the cellular metabolism than cadmium. However, combined exposure to cadmium and selenium increased the intensity of small intestine cell RNA synthesis. Combined cadmium/selenium exposure increased protein synthesis as compared with the animals receiving selenium only to the level similar to that observed in the controls.
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