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EN
Distribution of Dendrobaena alpina covers the mountainous region of central Europe, where it has a high dominance and frequency index. This work describes data from field studies conducted over two annual cycles in the Bieszczady National Park (BNP), Poland, in different types of beech forest. Densities of this species ranged from 47–231 g·m -2 with associated biomasses of 19–90 g·m -2. Most (66%) were recovered from less than 0.1 m soil depth with a further 28% from up to 0.2 m. Further, the animals were collected alive from Lutowiska, close to the BNP to obtain baseline data on the life history of D. alpina. Mature individuals were kept in isolation and cocoon production monitored on a 2 monthly basis. Immature individuals were kept until they reached maturity, when they were paired, with combined output of cocoons regularly monitored. Cocoons were collected, had their masses determined and were incubated. Hatchability was recorded, as was estimated duration of incubation and growth to maturity. Field-collected adults had a mean mass of 1.4 g and at 15 ºC, these produced an average of 1.83 cocoons·worm -1·month -1. Zero cocoons were produced by further specimens, collected as immature, grown to maturity and maintained in isolation. Recently-matured, paired D. alpina produced an average of 1.65 cocoons·worm -1·month -3. Cocoons had a mean mass of 19 mg. At 15 ºC, cocoons produced by recently matured adults hatched after 80 days (82% viable), but after 2 months the majority of cocoons failed to hatch. Hatchling growth from an average of 18 mg to maturity required 7–11 months in the given substrate.
EN
The greatest increases in S. bacillaris biomass were obtained in aerated and non-aerated cultures where the introduced inoculum contained 60-130 or 60-160 mg DW L-1, respectively. The application of inocula exceeding 160 mg DW L-1 brought about a rapid inhibition of biomass increase and growth rate, and also extended the lag phase. The dilution of the medium restored the ability of exponential growth in the culture. It seems that in cultures inoculated with large inocula the reduced growth of S. bacillaris was induced by autoinhibition.
PL
Największe przyrosty biomasy S. bacillaris uzyskano w hodowlach napowietrzanych i nienapowietrzanych, do których wprowadzono odpowiednio 60-130 i 60-160 mg DW L-1. Przy inoculum większym niż 160 mg DW L-1 następowało gwałtowne zmniejszenie przyrostu biomasy i tempa wzrostu, a także wydłużenie lag fazy. Rozcieńczenie kultury przywracało zdolność do wzrostu wykładniczego. Wydaje się, że przyczyną zahamowania wzrostu S. bacillaris w hodowlach szczepionych dużym inoculum mogła być autoinhibicja.
EN
Quantitative expressions are presented to describe the effects of temperature and food concentration on stage duration and growth rate of Temora longicornis for each of the model stage groups (N1–N6 – naupliar stages, C1, C2, C3, C4, C5 –the five copepodid stages). The calculations were made on the basis of experimental data from the literature for T. longicornis from the south-eastern and the southern North Sea. Relationships were obtained between the growth parameters and temperature for the 5–10◦C temperature range and food concentrations from 25 mgC m−3 to excess. Also computed was the total mean development time as a function of the above-mentioned parameters, temperature and food availability. The simulations computed here are similar to the experimental results. The growth rates for successive stages were obtained according to the correction of the ‘Moult Rate’ method, which allows the use of mean weights and stage durations. The calculations also suggest that three complete generations of T. longicornis from the Gdańsk Deep (the southern Baltic Sea) can develop during a single year.
EN
A nucleopolyhedrovirus isolated from the beet armyworm Spodoptera exigua (Polish laboratory culture), SeMNPV (P), morphologically similar to the viral bioinsecticide virus Spod-XR , was characterized molecularly and biologically. Phylogenetic analysis based on three conserved baculovirus genes, polh, lef-8 and pif-2, showed the highest homology of SeMNPV (P) to Mamestra brassicae (Mb) MNPV and M. configurata (Maco) MNPV, and much less to SeMNPV (Spod-XR). These findings were confirmed by genomic DNA restriction profile analyses. Bioassays revealed that SeMNPV isolated from the commercial bioinsecticide Spod-XR was themost infectious for S. exigua, while the infectivity of SeMNPV (P) and MbMNPV was significantly lower. These data suggest that SeMNPV (P) is a variant of MbMNPV.
PL
Scharakteryzowano pod względem molekularnym i biologicznym wirusa poliedrozy jądrowej (NPV) SeMNPV(P) pochodzącego z gąsienic światłołówki naziemnicy Spodoptera exigua (polska hodowla laboratoryjna) podobnego morfologicznie do aktywnego wirusowego składnika biopreparatu Spod-XR, SeMNPV(Spod-XR ). Analiza filogenetyczna polskiego izolatu wirusa oparta na trzech genach bakulowirusa polh, lef-8 i pif-2 wykazała niewielką homologię z wirusem SeMNPV(Spod-XR ) natomiast wysoką z wirusami Mamestra brassicae MbMNPV oraz Mamestra configurata MacoMNPV. Homologia ta została potwierdzona przez restrykcyjną analizę genomowego DNA. Wyniki testów biologicznych wykazały, że najbardziej aktywnym dla gąsienic S. exigua był wirus SeMNPV pochodzący z biopreparatu Spod-XR, natomiast aktywność wirusów SeMNPV-P i MbMNPV była porównywalnie niższa.
PL
Sprawdzono wpływ dodatku MgS04 7 H20 na wydajność biomasy komórkowej drożdży piekarskich. Zbadano również zdolność wiązania magnezu przez drożdże podczas hodowli na podłożu YPG i w warunkach przemysłowych. W skali przemysłowej ustalono, że celowe jest zwiększanie dodatku magnezu w poszczególnych propagacjach. W hodowli laboratoryjnej plon biomasy uzyskany z podłoża kontrolnego był istotnie mniejszy od plonów z hodowli doświadczalnych wzbogaconych w magnez (stężenie Mg 2+: 0,05; 0,3 i 0,5 g Mg2+ · dm-3 podłoża). Zaobserwowano, że wzrost zawartości magnezu w podłożu powodował zwiększenie ilości tego pierwiastka w biomasie komórkowej drożdży piekarskich.
EN
The influence of addition of MgS04-7H20 on biomass yield of Saccharomyces cerevisiae was evaluated in this study. Capacities of binding magnesium ions by S. cerevisiae yeast during cultivation on the YPG-medium (laboratory) and in the industrial scale were analyzed. It was stated that in the industrial conditions the higher amount of magnesium in the respective propagation is required. In the laboratory conditions the yield of yeast cultivated on the experimental media enriched with different amount (0,05, 0,3 and 0,5 g ·dm-3) of magnesium ions was also significantly higher than the yield from YPD - control medium. Growing concentration of magnesium ions in the experimental media caused the increasing of magnesium content in the yeast biomass.
EN
A series of larvae of Podothrombium reared in laboratory culture was subject to morphometric analysis. Larvae were assigned to P. filipes (C. L. Koch, 1837) a species known hitherto only from postlarval instars. An array of anomalies in the chaetotaxy of various body regions was discovered. The results make it possible to re-consider the taxonomic suitability of some characters commonly used in diagnosing larvae of Podothrombium.
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