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1 Medycyna Weterynaryjna

1 2014

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Zawartość dialdehydu malonowego i aktywność całkowitego stanu antyoksydacyjnego w płynie przedniej komory oka u psów z zaćmą starczą

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Madany J. , Milczak A. , Abramowicz B.

nr 10

Purpose: The aim of the study was to evaluate the content of malondialdehyde (MDA) and the activity of the total antioxidant status (TAS) in the aqueous humor of dogs with senile cataract without general conditions. Material and methods: The population examined consisted of 40 dogs of various breeds and sexes, aged 10-16 years, diagnosed with senile cataracts, which qualified for cataract surgery. Samples of the aqueous humor were collected at the beginning of the operation, after the opening of the anterior chamber. MDA luminescence spectra were determined with a Perkin-Elmer LS 30 by the method of Yagi. TAS was measured by a colorimetric reagent kit Randox Total Antioxidant Status. Results: Our results showed a significant increase in malondialdehyde (MDA) levels and a significantly lower activity of the total antioxidant status (TAS) in the aqueous humor of dogs with senile cataract compared to the control group. Conclusions: The results indicate an increased peroxidation of lipids and a local weakening of the defensive system in the aqueous humor of dogs with senile cataract. These results suggest that these conditions may have an impact on the development of age-related cataract, and are compatible with the free-radical theory of the development of senile cataract.