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1
Content available remote Improved high-performance liquid chromatography-DAD method for the simultaneous analysis of quercetin and kaempferol in the stems of Cissus quadrangularis Linn
100%
Thakur A. K. , Jain V. , Hingorani L. , Laddha K. S.
Acta Chromatographica
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2009
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tom Vol. 21, no. 1
95-103
EN
A simple, rapid, and specific reversed-phase HPLC method with DAD detection has been used for analysis of two flavonoids, quercetin and kaempferol, in Cissus quadrangularis Linn. The flavonoids were well resolved within 10 min, and quantification was achieved, on an endcapped C 18 column at 370 nm with acetonitrile-phosphate buffer (pH 3.4, adjusted with glacial acetic acid) 60:40 (v/v) as isocratic mobile phase at a flow rate of 1.0 mL min -1. The method was validated for limit of detection, limit of quantification, linearity, precision, accuracy, and recovery. Linearity was demonstrated over the range 0.75 to 10 µg mL -1 for quercetin and 1.0 to 10 µg mL -1 for kaempferol with good correlation coefficients ( r 2 > 0.998). Detection limits were 0.075 and 0.10 µg mL -1 for quercetin and kaempferol respectively. Recovery was 98.0-105.3% for quercetin and 95.0-101.1% for kaempferol. The method was successfully applied to analysis of two flavonoids in four samples of Cissus quadrangularis Linn.
2
Content available remote Validated HPTLC method for simultaneous quantification of sennoside A, sennoside B, and kaempferol in Cassia fistula Linn.
88%
Bhope S. G. , Kuber V. V. , Nagore D. H.
Acta Chromatographica
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2010
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tom Vol. 22, no. 3
481--489
EN
This paper describes a simple, precise, and accurate HPTLC method for simultaneous quantification of sennoside A, sennoside B, and kaempferol in Cassia fistula whole plant extract. Chromatographic separation of the sample extract was performed on aluminium foil plates coated with silica gel 60 F 254 as stationary phase. The mobile phase was toluene-ethyl acetate-methanol-formic acid 8:10:5:2 ( υ/υ ). Densitometric evaluation of the separated bands was performed at 270 nm. Sennosides A and B and kaempferol were satisfactorily resolved at R F 0.22 ± 0.05, 0.19 ± 0.05, and 0.81 ± 0.05, respectively. Recovery of sennosides A and B and kaempferol from Cassia fistula extract was 98.03, 98.74, and 99.08%, respectively. The method was validated for specificity, accuracy, linearity (100–400 ng per band), and precision (instrument precision in the range 1.03–1.33 and method precision in the range of 1.31–1.75) in accordance with ICH guidelines.
3
Content available Validation of the analytical method for the determination of flavonoids in broccoli
75%
Tuszynska M.
Journal of Horticultural Research
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2014
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tom 22
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nr 1
4
Content available Antioxidant Activity and Mechanism in Flower of Hylocereus Undatus (Haw.) Britt. Et Rose
75%
Li X. , Gao Y. , Han W. , Lin J. , Hu Q. , Chen D.
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5
Content available remote Detection and quantification of Naringenin and kaempferol in Melastoma decemfidum extracts by GC-FID and GC-MS
75%
Sarju N. , Abd Samad A. , Abd Ghani M. , Ahmad F.
Acta Chromatographica
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2012
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tom Vol. 24, no. 2
221--228
EN
Melastoma decemfidum is a plant species from the Melastomataceae family. The plant was reported to have bioactive flavonoids, which showed antioxidant and cytotoxic activities. Extracts from the leaves of 26 plants were made at room temperature with methanol. Detection and quantification of two of the flavonoids, namely naringenin and kaempferol, in the extracts were carried out by using gas chromatography-flame ion detection (GC-FID) and gas chromatography-mass spectrometry (GC-MS). By optimizing the key experimental parameters, a linear response for the individual target compounds was obtained in the concentration range LOQ from 3.44 to 8.26 g mL -1 (r2 = 0.9731–0.9772), with LODs from 1.13 up to 2.72 μg mL-1 per 1.0 g of crushed leaves, and with repeatability within the relative standard deviation (RSD) of 1.65–1.81%.
6
Changes of the flavonols content in onion during the vegetation period and storage
75%
Horbowicz M.
Vegetable Crops Research Bulletin
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1999
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tom 50
7
Content available remote Simultaneous determination of gallic acid, protocatechuic acid, catechin, epicatechin, quercetin and kaempferol in Chinese chestnut (Castanea mollissima blume) kernel by high-performance liquid chromatography with diode array detection
63%
Wang T. , Yang Y. D. , Du B. , Yu S. , Hou W. L.
Acta Chromatographica
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2014
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tom Vol. 26, no. 3
539--550
EN
Chestnut exhibits anti-inflammatory, styptic, anti-diarrhea, and analgestic effects as a traditional Chinese medicine. There is increasing evidence that shows that the consumption of chestnuts has become more important in human nutrition due to the health benefits provided by the antioxidants. The phenolic compounds are responsible for major bioactivities, such as anti-tumor and anti-oxidation. A high-performance liquid chromatography (HPLC) method with diode array detection (DAD) was established for the simultaneous determination of six phenolic compounds (gallic acid, GA; protocatechuic acid, PR; catechin, CA; epicatechin, EP; quercetin, QU; kaempferol, KA) in Chinese chestnut (Castanea mollissima blume) kernel. The sample followed by separation on Eclipse XDB-C18 column (150 × 4.6 mm, id., 5 μm) with gradient elution of methanol-1.0% acetate acid solution as a mobile phase, at a temperature of 30°C, under the ratio of 1.2 mL min-1, with 5 μL injection volume, and multi-wavelength synthesis was used with DAD. The correlation coefficients were larger than 0.999, the recoveries were 97.58% for GA, 100.41% for PA, 96.23% for CA, 101.38% for QU, 99.15% for EP, and 98.60% for KA, relative standard deviation (RSD) were 1.04% for GA, 1.21% for PA, 1.09% for CA, 1.19% for QU, 1.06% for EP, and 1.20% for KA. This method was applied for the determination of phenolics in chestnut kernel and was found to be fast, sensitive, and suitable.
8
Content available In silico assessment of the inhibitory effect of four flavonoids (chrysin, naringin, quercetin, kaempferol) on tyrosinase activity using the MD simulation approach
63%
Farasat A. , Ghorbani M. , Gheibi N. , Shariatifar H.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
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2020
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tom 101
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nr 3
9
Content available remote Simultaneous HPLC-DAD analysis of five flavonoids in diabetic rat plasma and its application in the study of pharmacokinetics
63%
Tang D. , Yin Y. , Zhang Z. , Gao Y. , Wei Y. , Chen Y. , Han Y.
Acta Chromatographica
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2009
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tom Vol. 21, no. 3
483--497
EN
A HPLC-DAD method for simultaneous analysis of five flavonoids (rutin, quercitrin, quercetin, kaempferol, and isorhamnetin) in diabetic rat plasma has been developed and validated. Separation of the five flavonoids was accomplished on a C 18 column (250 mm × 4.6 mm i.d., 5-µm particle) and detection was performed at 350 nm. The best resolution was achieved with a methanol-0.1% formic acid gradient at a flow rate of 1.0 mL min −1 . The correlation coefficients for all the calibration plots ( r > 0.999) showed linearity was good over the range tested. The relative standard deviation of the method was less than 7% and 10% for intra- and inter-day assays, and average recovery was between 77.2 and 99.2%. Sensitivity was high and detection limits were between 0.006 and 0.02 µg mL −1 . The method has been successfully used to determine drug concentrations in diabetic rat plasma samples and the pharmacokinetics of the drugs.
10
Content available Antioxidant activity and mechanism in flower of Hylocereus undatus (Haw.) Britt. et Rose
63%
Li X. , Gao Y. , Han W. , Lin J. , Hu Q. , Chen D.
Acta Biologica Cracoviensia. Series Botanica
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2013
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tom 55
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nr 1
EN
Hylocereus undatus flower is commonly used as food or for medicinal purposes in south China. To study its antioxidant activity and mechanism we used antioxidant and chemical assays to compare two commercial samples from different locations (Shenjing, Qixing). The difference in antioxidant levels corresponded with differences in chemical content (including total phenolics, total flavonoids, kaempferol and quercetin) between Shenjing and Qixing. The antioxidant ability of H. undatus flower seems attributable to total phenolics (mainly total flavonoids). Kaempferol is one of the main bioactive components. H. undatus flower exerts its antioxidant effects through metal chelation and radical scavenging via hydrogen atom (H•) and electron (e) donation.
11
Content available remote Kaempferol, but not resveratrol inhibits angiotensin converting enzyme
63%
Olszanecki R. , Bujak-Gizycka B. , Madej J. , Suski M. , Wolkow P. P. , Jawien J. , Korbut R.
Journal of Physiology and Pharmacology
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2008
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tom 59
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nr 2
EN
Inhibition of angiotensin converting enzyme (ACE) has proved to be beneficial in the treatment of various cardiovascular disorders. The aim of this study was to evaluate ACE inhibitory potential of two polyphenolic compounds with different structures: resveratrol (present in high quantities in French wine) and kaempferol (abundant in greens), using method of liquid chromatography coupled with electrospray ionization mass spectrometry (LC-ESI-MS) for ex vivo measurement of angiotensin I to angiotensin II conversion by ACE in aortic tissue of Wistar-Kyoto rats. In this setting, kaempferol (10-30-100 µM), but not resveratrol (10-30-100 µM) appeared to inhibit dose-dependently conversion of Ang I to Ang II. Although the mechanism of ACE inhibition by kaempferol remains to be elucidated, this observation may help in search or designing of new classes of ACE inhibitors.
12
Changes of flavonols content during vegetation period and storage of shallot [Allium cepa var.aggregatum]
63%
Horbowicz M. , Kotlinska T.
Vegetable Crops Research Bulletin
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2001
|
tom 55
13
Content available Therapeutic potential of flavonoids used in traditional Chinese medicine – a comparative study of galangin, kaempferol, chrysin and fisetin
51%
Sokal A. , Stocerz K. , Olczyk P. , Kadela-Tomanek M.
Annales Academiae Medicae Silesiensis
|
2023
|
nr 78
49-60
PL
Ziołolecznictwo stanowi jedną z głównych gałęzi tradycyjnej medycyny chińskiej (traditional Chinese medicine – TCM). W pracy skupiono się na aktywności biologicznej wybranych flawonoidów, a także konkretnych przykładach wpływu tych substancji na różne układy organizmu. Flawonoidy to grupa związków chemicznych zawartych w surowcach roślinnych, miodzie, propolisie czy grzybach stosowanych w TCM. Chryzyna, galangina, kemferol i fisetyna to przykłady flawonoidów wykazujących m.in. właściwości przeciwutleniające, przeciwzapalne czy przeciwbakteryjne. Właściwości te są przedmiotem wielu badań naukowych, mających na celu zbadanie ich potencjalnego działania terapeutycznego.
EN
One of the branches of traditional Chinese medicine (TCM) is herbal medicine. In this paper, we focus on the biological activity of substances belonging to flavonoids and specific examples of their impact on various body systems. Flavonoids are a group of chemical compounds included in plant materials, honey, propolis or mushrooms used in TCM. Chrysin, galangin, kaempferol and fisetin are examples of flavonoids showing, among others, antioxidant, anti-inflammatory or antibacterial properties, which are the subject of various scientific studies aimed at examining their potential therapeutic effect.
14
Content available Assessment of antiproliferative, cytotoxic and proapoptotic properties of selected flavonoids in relation to liver cancers – in vitro studies
51%
Skalny T. , Kleczka A. , Kubina R. , Owczarzy A. , Wyszyńska M. , Kabała-Dzik A.
Annales Academiae Medicae Silesiensis
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2020
|
nr 74
181-190
EN
INTRODUCTION: Flavonoids belong to phytotherapeutics with a wide spectrum of pharmacological activity. It has been proven that flavonoids possess properties that can inhibit the development of cancer by inducing cells into the programmed cell death process. The aim of the work was to demonstrate the apoptotic, antiproliferative and cytotoxic properties of selected flavonoids. MATERIAL AND METHODS: The test material was the human primary hepatocellular carcinoma SK Hep-1 cell line. The tested compounds were assessed for cytotoxicity with the MTT assay. The next step was to evaluate the level of protein expression from the Bcl-2 family using the human Bcl-2 ELISA test. RESULTS: The compound with the strongest cytotoxic properties confirmed by the MTT test is chrysin, the IC50 value of which was 316.67 μM/L. In the case of all the tested compounds, apoptotic processes were confirmed by the human Bcl-2 ELISA test. The highest level of Bcl-2 protein expression occurred after 48 hours after the administration of chrysin, hesperidin, naringin and kaempferol. CONCLUSIONS: On the basis of the obtained research results, it can be concluded that the studied flavonoids (chrysin, hesperidin, naringin, kaempferol) exhibit cytotoxic, proapoptotic and antiproliferative properties in relation to SK Hep-1 hepatoma cells.
PL
WSTĘP: Flawonoidy należą do fitoterapeutyków o szerokim spektrum działania farmakologicznego. Udowodniono, że flawonoidy posiadają właściwości mogące hamować rozwój choroby nowotworowej poprzez wywoływanie w komórkach procesu programowanej śmierci komórki. Celem pracy było wykazanie właściwości apoptotycznych, antyproliferacyjnych oraz cytotoksycznych wybranych flawonoidów. MATERIAŁ I METODY: Materiał do badań stanowiła ludzka linia komórkowa pierwotnego raka wątrobowokomórkowego. Badane związki poddano ocenie cytotoksyczności za pomocą testu MTT. Kolejnym etapem była ocena poziomu ekspresji białek z rodziny Bcl-2 testem Human Bcl-2. WYNIKI: Związkiem posiadającym najsilniejsze właściwości cytotoksyczne, potwierdzone testem MTT, jest chryzyna, której wartość IC50 wyniosła 316,67 μM/L. W przypadku wszystkich badanych związków stwierdzono zainicjowanie procesów apoptotycznych potwierdzone testem Human Bcl-2 ELISA. Najwyższy poziom ekspresji białek Bcl-2 miał miejsce po upływie 48 godzin od podania chryzyny, hesperydyny, narynginy i kemferolu. WNIOSKI: Na podstawie uzyskanych wyników badań można wnioskować, że badane flawonoidy (chryzyna, hesperydyna, naryngina, kemferol) wykazują właściwości cytotoksyczne, proapoptotyczne i antyproliferacyjne w stosunku do komórek raka wątrobowokomórkowego SK Hep-1.
15
Antioxidative activity of flavonoids in stimulated human neutrophils
51%
Zielinska M. , Kostrzewa A. , Ignatowicz E.
Folia Histochemica et Cytobiologica
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2000
|
tom 38
|
nr 1
16
Flavonoids in white and yellow perianths and yellow anthers of tulips [Tulipa gesneriana L.]
51%
Horbowicz M. , Saniewski M.
Acta Agrobotanica
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2001
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tom 54
|
nr 2
EN
The content of flavonoids in white and yellow perianths and yellow anthers of a few tulip cultivars were determined at the stage of full flowering. To analyses of flavonols a HPLC method was used. In anthers (yellow) of all analyzed cultivars (Oscar, Pax, Profesor Wóycicki, Biała Dama, White Virgin, Calypso, Diana) high content of quercetin (2.35 - 6.01 mg . g-l EW), kaempferol (1.09 - 9.47 mg . g.1 EW) and apigenin (1.34 - 8.24 mg• g-l EW.) was found. In analyzed white perianth of cvs. Oscar and White Virgin also high content of quercetin (1.3 - 1.80 mg . g-l EW) and kaempferol (1.90 mg . g-l EW.) was documented and only traces of apigenin was found. In the yellow perianth of cv. Profesor Wóycicki the level of quercetin and kaempferol was much lower than in perianth of cvs. Oscar and White Virgin, and apigenin was absent.Thus, yellow anthers and white and yellow perianth of tulip cultivars are a rich source of flavonols.
PL
Analizowano zawartość flawonoidów (metodą HPLC) w białym i żółtym okwiecie i w żółtych pylnikach kilku odmian tulipanów w okresie pełni kwitnienia. W żółtych pylnikach analizowanych odmian (Oscar, Pax, Profesor Wóycicki, Biała Dama, White Virgin, Calypso, Diana) wykazano wysoką zawartość kwercetyny (2,35 - 6,01 mg • g-1 ś.m.), kempferolu (1,09 - 9,47 mg • g-1 ś.m.) i apigeniny (1,34 - 8,24 mg . g-1 ś.m.). W białym okwiecie odmian Oscar i White Virgin stwierdzono również wysoki poziom kwercetyny (1,30- 1,80 mg . g-1 ś.m.) i kempferolu (1,90 mg • g-1 ś.m.), a tylko ilości śladowe apigeniny. W żółtym okwiecie odmiany Profesor Wóycicki zawartość kwercetyny i kempferolu była dużo mniejsza niż w białym okwiecie odmian Oscar i White Virgin, a brak było apigeniny. Tak więc żółte pylniki i biały i żółty okwiat odmian tulipanów są bogatym źródłem flawonoidów.
17
Antioxidant properties of highbush blueberry fruit cultivars
44%
Scibisz I. , Mitek M.
|
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tom 10
|
nr 4
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