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EN
Intestinal parasitic diseases in Gaza Strip are a significant health problem. The main aim of this study is to determine the prevalence of parasitic infection among patients in the five governorates of the Gaza Strip. A crosssectional parasitological survey was conducted on 600 patients. Stool specimens were examined microscopically for the presence of parasite eggs or larvae/cysts per wet amount by the saline iodine and formol-ether concentration method. Of 600 subjects examined, 245 (40.8%) were infected with one or more intestinal parasites. Entamoeba histolytica/dispar and Giardia lamblia were the most prevalent parasites amongst the population (28.8%), (9.5%). Female patients demonstrated a higher prevalence of intestinal parasitic infections (42.7%) than males (39.0%). However, this difference was not significant (p>0.05). According to occupation, the rate of infection was highest among farmers (56.0%), followed by employers (44.2%) with laborers showing the lowest rate (30.17%). These differences in occupational prevalence were found to be statistically significant (p<0.05). The present study demonstrates that intestinal protozoan infections are still a public health problem in the Gaza strip, with Entamoeba and Giardia infections being most common. Therefore, it is necessary to develop effective prevention and control strategies, including health education and environmental sanitation improvement.
EN
The scolex of the bothriocephalidean cestode Clestobothrium crassiceps was studied by means of scanning electron microscopy (SEM). The comparative results of various fixation procedures and techniques are presented. The scolex of C. crassiceps is oval to globular and exhibits two deep bothria which appear in the form of two lobes separated by a longitudinal groove. At the apex of the scolex, resembling a beret, an apical disc is present (oval, flattened and with a sinuous edge). Our results are compared with those previously reported in other species of Clestobothrium. This study represents the first report which highlights the presence of an apical disc in the scolex of C. crassiceps. It describes the effects of different procedures applied to our material during preparation and a comparative analysis results obtained using these various methods.
EN
The aim of the study was to determine the prevalence of infection with gastrointestinal parasites in Polish Konik horses from Poland and those imported from the Netherlands. The prevalence and rate of infection was determined based on coproscopic examination using Willis-Schlaf and Mc-Master methods. Faecal samples were tested for the presence of Cryptosporidium sp. using a modified Ziehl-Neelsen staining method. Mean prevalence of infection with gastrointestinal parasites in Polish Koniks imported from the Netherlands and those from Poland was 100%. Imported horses were found to harbour nematodes of the family Strongylidae (89.47%) and Cyathostominae (94.74%) as well as Parascaris equorum (5.26%) roundworms. Domestic Polish Koniks were found to harbour Strongylidae (100%) and Cyathostominae (100%) nematodes as well as Cryptosporidium sp. protozoa (2.27%). Domestic horses were more infected with Cyathostominae (865 EPG) and Strongylidae (731 EPG) than horses imported from the Netherlands (739 and 600 EPG, respectively). The study demonstrated that Polish Koniks from Poland and those imported from the Netherlands should be monitored parasitologically because endoparasites may create a major epizootiological problem when these animals are kept in an organic production system.
EN
Echinoparyphium species are common, widely distributed intestinal parasites causing disease in animals worldwide. Intermediate hosts include snails, bivalves, and fish, whereas the definitive hosts are mainly birds and mammals. This review examines the significant literature on Echinoparyphium. Descriptive studies, life cycle, experimental and manipulative studies, and biochemical and molecular studies are presented. The influence of environmental factors, and toxic pollutants, are reviewed as well as studies on the pathology of Echinoparyphium.
EN
Acalculous cholecystitis and cholangitis are increasingly being recognized as complications of AIDS. The opportunistic parasites that have been most commonly associated with these disorders are Cryptosporidium species, Isospora belli, Cyclospora cayetanensis and Enterocytozoon bieneusi. The authors performed a parasitological survey on the gallbladder tissue sections of patients underwent cholecystectomy due to chronic acalculous cholecystitis at the Shiraz University of Medical Sciences, Iran. Light microscopic investigation in more than three hundred archived histopathological slides revealed the presence of sexual stages (i.e., mature sporocysts) of a coccidial protozoan in a patient with AIDS who developed acalculous cholecystitis as confirmed by histological, parasitological and molecular tests in which Sarcocystis species was the only identifiable pathogen in gallbladder sections. In the best of our knowledge it’s the first documented case of chronic non-calculous cholecystitis due to Sarcocystis parasite in an Iranian AIDS patient from worldwide.
EN
The unhatched, newly hatched and free-swimming coracidia of Bothriocephalus clavibothrium Ariola, 1899 an intestinal parasite of the teleostean fish Arnoglossus laterna (Walbaum, 1792) (Pleuronectidae) from the Mediterranean Sea near Séte, France were examined by means of transmission electron microscopy (TEM) and cytochemistry methods. Each unhatched and hatched coracidium of B. clavibothrium is composed of a hexacanth larva, oncosphere, about 28 µm in diameter, surrounded by a 5-7 µm thick ciliated envelope containing about 16 mesomere nuclei and a large amount of nutritive reserves (α- and ß-glycogen and lipids). Several cell types were distinguished within the oncosphere: (1) binucleated subtegumental cell; (2) two flame cells; (3) binucleated penetration gland; (4) two nerve cells; (5) about 100 intermediate, somatic cells which represent perikarya of both somatic and hook musculature; (6) about 50 small embryonic cells, some of them with pycnotic nuclei containing very dense chromatin and showing evident signs of regression and degeneration; and (7) about 10 to 12 germinative cells with prominent nucleoli in large lobate nuclei, surrounded by a thin layer of compact, granular cytoplasm rich in RNA. Apoptosis of numerous micromeres and of outer-envelope and ciliated-envelope material was frequently observed. The total number of oncospheral cells is about 160, the highest number reported from various orders and families of cestodes. This number, along with our own data from different cestode orders and families, supports our hypothesis that the progressive reduction in oncosphere cell numbers is correlated with a simplification of the infective larval stages and is a general trend in cestode evolution. This correlation represents an interesting ontogenic adaptation to the parasitic way of life.
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