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  1. 19 Acta Parasitologica
  2. 5 Acta Protozoologica
  3. 2 Acta Biologica Cracoviensia. Series Botanica
  4. 2 BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
  5. 2 Journal of Plant Protection Research
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  1. 1 2021
  2. 4 2020
  3. 1 2019
  4. 9 2018
  5. 5 2017
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  1. 4 Li L.
  2. 3 Zhang L.-P.
  3. 2 Chen H.-X.
  4. 2 Gomez F.
  5. 2 Zhang L.
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1
Content available Identification of medicinal plant Schisandra chinensis using a potential DNA barcode ITS2
100%
Li X.-K. , Wang B. , Han R.-C. , Zheng Y.-C. , Yin H.-B. , Xu L. , Zhang J.-K. , Xu B.-L.
Acta Societatis Botanicorum Poloniae
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2013
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tom 82
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nr 4
EN
To test whether the internal transcribed spacer 2 (ITS2) region is an effective marker for using in authenticating of the Schisandra chinensis at the species and population levels, separately. And the results showed that the wild populations had higher percentage of individuals that had substitution of C→A at site 86-bp than the cultivated populations. At sites 10-bp, 37-bp, 42-bp and 235-bp, these bases of the Schisandra sphenanthera samples differed from that of S. chinensis. Two species showed higher levels of inter-specific divergence than intra-specific divergence within ITS2 sequences. However, 24 populations did not demonstrate much difference as inter-specific and intra-specific divergences were concerned. Both S. chinensis and S. sphenanthera showed monophyly at species level, yet the samples of different populations shown polyphyly at population level. ITS2 performed well when using BLAST1 method. ITS2 obtained 100% identification success rates at the species level for S. chinensis, with no ambiguous identification at the genus level for ITS2 alone. The ITS2 region could be used to identify S. chinensis and S. sphenanthera in the “Chinese Pharmacopoeia”. And it could also correctly distinguish 100% of species and 100% of genera from the 193 sequences of S. chinensis. Hence, the ITS2 is a powerful and efficient tool for species identification of S. chinensis.
2
Content available Molecular analysis of Sorbus sp. from the Pieniny Mts. and its relation to other sorbus species
100%
Dluzewska J. , Slezak I. , Kruk J.
Acta Biologica Cracoviensia. Series Botanica
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2013
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tom 55
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nr 1
EN
The systematic position of Sorbus population occurring in the Pieniny Mts. is controversial. To verify its taxonomic status we studied the ITS sequence of closely related species of the S. aria group: Sorbus sp. from the Pieniny Mts., S. aria from the Tatra Mts., S. graeca from the Balkans, and other well-distinguished native Polish Sorbus species (S. aria, S. aucuparia, S. intermedia and S. torminalis). As a reference we examined Sorbus populations closest to the Pieniny Mts. where S. graeca was reported to occur, in Slovakia. The results indicate that the Sorbus plants found in the Pieniny Mts. differ genetically from those in the Tatra Mts. but are identical to those collected from the Vihorlat Mts. in Slovakia and are closely related to S. graeca from the Balkans.
3
Morphological and molecular identification of Paramphistomum epiclitum from buffaloes in Pakistan
86%
Khan I. , Afshan K. , Shah S. , Akhtar S. , Komal M. , Firasat S.
Acta Parasitologica
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2020
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tom 65
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nr 1
4
Phylogenetic analysis of genetically distinct Enterocytozoon bieneusi infecting renal transplant recipients
86%
Khanduja S. , Ghoshal U. , Ghoshal U. C.
Acta Parasitologica
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2017
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tom 62
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nr 1
5
Allovahlkampfia minuta nov. sp., (Acrasidae, Heterolobosea, Excavata) a new soil amoeba at the boundary of the acrasid cellular slime moulds
72%
De Obeso Fernadez del Valle A. , Maciver S. K.
Acta Protozoologica
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2017
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tom 56
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nr 3
6
A PCR method targeting internal transcribed spacers: the simultaneous detection of Babesia bigemina and Babesia bovis in cattle
72%
Liu J. , Guan G. , Liu A. , Li Y. , Yin H. , Luo J.
Acta Parasitologica
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2014
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tom 59
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nr 1
XX
In this study, two pairs of oligonucleotide primers were designed according to the nucleotide sequence of the internal transcribed spacers (ITSs) of Babesia bigemina and B. bovis isolates from China. The primers were used in a multiplex PCR to detect parasite DNA in blood samples from cattle. There was no cross reactions with B. ovata, B. major, B. sp. Kashi, Theileria annulata, T. sergenti, T. sinensis or normal bovine DNA. The sensitivity of multiplex PCR assay was 1 pg and 10 pg DNA for B. bigemina and B. bovis, respectively. A total of 260 field blood samples collected from cattle in five provinces of China were analyzed by multiplex PCR and light microscopy. PCR testing revealed that 7.3% (19/260) and 5.8% (15/260) of cattle were positive for B. bigemina and B. bovis and 1.2% (3/260) of cattle were co-infected with B. bigemina and B. bovis. Using light microscopy, 2.3% (6/260) and 1.5% (4/260) of cattle were infected by B. bigemina and B. bovis, respectively, and no co-infection was found. The results showed that the multiplex PCR developed in the present study could be an alternative diagnostic tool for the detection of B. bovis and B. bigemina infection in cattle.
7
Content available Identification of Vietnamese Ochna integerrima (Lour.) merr species based on ribosomal DNA internal transcribed spacer sequence
72%
Trang D. H. , Van Dong D. , Chung B. H. , Gioi D. H. , Khanh T. D.
International Letters of Natural Sciences
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2018
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tom 68
EN
Ochna integerrima is a medicinal and ornamental plant, is widely distributed in Southeast Asia areas. In Vietnam, it has been ranked as the rare and endangered species due to its high demand trade of the beautiful species. In this study, total 21 Ochna samples, collected from the northern and southern areas, were used to characterize the morphological traits using morphological analyses and molecular tool. The results have revealed that the morphological characterization of flower and its quality of Yen Tu Ochna samples showed differences in comparison with the common Ochna and southern Ochna samples. To accurately distinguish genetic traits of the samples, we have sequenced the internal transcribed spacer (ITS) region (including ITS1, 5.8S) of 21 species. The results have disclosed the genetic correlations of the samples ranging from 96.25% to 100% among the studied Ochna samples, of which 5 samples include B1, B2, B3, B6 and N3.1 were divided into the separate groups. The current work is the first report in constructing a molecular database of Ochna for further assessment of inter-and intra-specific molecular diversity of Ochna species in Vietnam.
8
Isolation, identification and characterization of the nematophagous fungus Arthrobotrys (Monacrosporium) sinense from China
72%
Xue Y.-J. , Li E.-L. , Jing C.-X. , Ma L. , Cai K.-Z.
Acta Parasitologica
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2018
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tom 63
|
nr 2
9
Ascaridia galli isolates with ITS1-5.8rRNA-ITS2 fragment homologous to Ascaridia columbae
72%
Urbanowicz J. , Gawel A. , Bobrek K.
Acta Parasitologica
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2018
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tom 63
|
nr 3
10
Differentiation of Toxocara canis and Toxocara cati based on PCR-RFLP analyses of rDNA-ITS and mitochondrial cox1 and nad1 regions
72%
Mikaeili F. , Mathis A. , Deplazes P. , Mirhendi H. , Barazesh A. , Ebrahimi S. , Kia E. B.
Acta Parasitologica
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2017
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tom 62
|
nr 3
11
First report of molecular identification of Cystoisospora suis in piglets with lethal diarrhea in Japan
72%
Matsubayashi M. , Takayama H. , Kusumoto M. , Murata M. , Uchiyama Y. , Kaji M. , Sasai K. , Yamaguchi R. , Shibahara T.
Acta Parasitologica
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2016
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tom 61
|
nr 2
12
Content available Problems, limitations, and challenges in species identifcation of Ascomycota members on the basis of ITS regions
72%
Baturo-Ciesniewska A. , Pusz W. , Patejuk K.
Acta Mycologica
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2020
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tom 55
|
nr 1
13
New haplotypes of Trypanosoma evansi identified in dromedary camels from Algeria
72%
Boutellis A. , Bellabidi M. , Benaissa M. H. , Harrat Z. , Brahmi K. , Drali R. , Kernif T.
Acta Parasitologica
|
2021
|
tom 66
|
nr 1
14
Morphological and molecular characterization of Seuratascaris numidica (Seurat, 1917) (Ascaridida: Ascarididae)
72%
Chen H.-X. , Zhang K. , Zhang L.-P. , Li L.
Acta Parasitologica
|
2018
|
tom 63
|
nr 1
15
First molecular detection and phylogenetic analysis of Neospora caninum DNA from naturally infected goats in Northwest Tunisia
72%
Amdouni Y. , Amairia S. , Said Y. , Awadi S. , Gharbi M.
Acta Parasitologica
|
2018
|
tom 63
|
nr 4
16
A new identification method for Clarias gariepinus, Clarias macrocephalus and their hybrids using PCR-RFLP
72%
Panicz R. , Sadowski J. , Hofsoe-Oppermann P. , Polgesek M.
Electronic Journal of Polish Agricultural Universities. Series Fisheries
|
2018
|
tom 21
|
nr 4
17
Morphological and molecular study of Neorhadinorhynchus nudus (Harada, 1938) (Acanthocephala: Cavisomidae) from Auxis thazard Lacepede (Perciformes: Scombridae) in the South China Sea
72%
Li L. , Chen H.-X. , Yang Y.
Acta Parasitologica
|
2018
|
tom 63
|
nr 3
18
Content available Screening and identification of novel cellulolytic Trichoderma species from Egyptian habitats
72%
Hewedy O. A. , El-Zanaty A. M. , Fahmi A. I.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
|
2020
|
tom 101
|
nr 2
19
Phylogenetic analysis of the genus Cylicocyclus (Nematoda: Strongylidae) based on nuclear ribosomal sequence data
72%
Bu Y. , Niu H. , Zhang L.
Acta Parasitologica
|
2013
|
tom 58
|
nr 2
EN
Seven species of Cylicocyclus Ihle, 1922 (Nematoda: Strongylidae) were collected from donkeys from Henan Province, China. Five samples of each species were selected for sequencing. Sixteen different internal transcribed spacer (ITS) sequences representing the seven species of Cylicocyclus were obtained. Sequence differences in the first internal transcribed spacer (ITS-1) among species was lower than that of the second internal transcribed spacer (ITS-2). Phylogenetic analyses were conducted using the combined ITS-1 and ITS-2 data sets from the present study and using reference sequences from the GenBank database. The MP and ML trees were similar in topology. The phylogenetic trees were divided into two clades. Clade I included 8 species of Cylicocyclus; within this group, Cylicocyclus leptostomus (Kotlan, 1920) is nested between different samples of Cylicocyclus ashworthi (LeRoux, 1924), suggesting C. ashworthi may represent a species complex. Clade II included Cylicocyclus elongatus (Looss, 1900) and Cylicocyclus ultrajectinus (Ihle, 1920); however, these two species always clustered with the comparative species (Petrovinema poculatum (Looss, 1900) and Poteriostomum imparidentatum Quiel, 1919), suggesting that C. elongatus and C. ultrajectinus represent members of other genera.
20
Content available The preliminary stage of SCAR markers development for Ranunculus subgen. Batrachium
72%
Jopek M.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
|
2013
|
tom 94
|
nr 3
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