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EN
The paper contains the description of mass poisoning of honey bees and the method used to determine dichlorodiphenyltrichloroethane, methoxychlor, and lindane in the insects. The ultrasound-aided extraction of pesticides from dead bees was conducted using dichloromethane and the analysis of the extracts was conducted using gas chromatography-mass spectrometry. The whole procedure was vatidated.
EN
Insecticides have leading role in reduction of population of arthropods, especially vectors of infectious diseases. Efficacy of insecticides is usually proved on biological material and described after adjusting the values of parameters in some “theoretical” function to the experimental results. In the standard approach, such function is described by probit regression. However, in some cases reaction of the population on pesticide differs from those which could be described by the probit regression curve and the results could not be properly assessed. The aim of this study was to elaboratie a practical tool for evaluating insecticide/acaricide efficacy, more universal than classical probit calculation. Software performing analysis was created using R 2.13.1 statistical software.
4
Content available A short history of insecticides
100%
EN
This review contains a brief history of the use of insecticides. The peculiarities, main advantages, and disadvantages of some modern insecticides are described. The names of the discoverers of some of the most popular insecticide preparations on the world market, are listed. The tendencies to find new insecticides to control the quantity of phytophagous insects are discussed. Special attention is paid to the perspective of creating preparations based on nucleic acids, in particular DNA insecticides. The use of insect-specific, short single-stranded DNA fragments as DNA insecticides, is paving the way in the field of “intellectual” insecticides that “think” before they act. It is worth noting, though, that in the near future, the quantity of produced insecticides will increase due to the challenges associated with food production for a rapidly growing population. It is concluded, that an agreeable interaction of scientists and manufacturers of insecticides should lead to the selection of the most optimal solutions for insect pest control, which would be safe, affordable, and effective at the same time.
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tom 57
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nr 4
EN
Products based on different strains of entomopathogenic fungi are now being used in Integrated Pest Management (IPM) programs. Compatibility studies of chemical and biological control agents are necessary to be able to give proper recommendations for their integrated use. The effect of three insecticides based on imidacloprid, spinosad and abamectin, and three fungicides based on chlorothalonil, azoxystrobin and thiophanate-methyl on the activity of the following entomopathogenic fungi: Metarhizium anisopliae (Metschn.) sensu lato, Beauveria bassiana (Bals.-Criv.) Vuill., Acremonium sp. was tested under laboratory conditions. Tests of the influence of the pesticides on growth and production of conidia were performed. From this study, we concluded that all tested insecticides can be applied together with fungus B. bassiana products in IPM programs. They even stimulate sporulation of this fungus at the recommended dose, 0.5 of the recommended dose and 1.5 times the recommended dose. In the case of fungicides we observed inhibition of growth and sporulation of B. bassiana and reduction of growth and sporulation of other species of fungi.
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tom 09
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nr 1
EN
Polychlorinated biphenyls (PCBs) and chloroorganic insecticides interfere with gas chromatographic analysis, thereby complicating their quantitative determination when multiple compounds are assayed. Perchlorination of the standards of the chlorinated compounds was used to simplify quantitative determination of these contaminants. Results indicate that perchlorination converts PCBs to a single compound (decachlorobiphenyl – DCB) that does not interfere with the assay for chloroorganic insecticides. These data confirm that perchlorination allows for simultaneous testing of both PCBs and chloroorganic insecticides during chromatographic analysis.
EN
Attempts to obtain a micro-dispersion-based formulation insecticide were made. An aqueous micro-suspension of polystyrene had been used to trap pyrethroid lambda-cyhalothrin. A method of introducing the active substance into the polymer was developed. The biological efficiency of micro-emulsion of lambda-cyhalothrin with polystyrene was investigated.
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tom nr 3-4
74--77
PL
Na ogół przez pestycydy rozumie się środki chemiczne o szerokim spektrum działania biobójczego, zapewniające efektywne zwalczanie organizmów przynoszących poważne straty w gospodarce. Niestety, większość takich związków chemicznych ma charakter ksenobiotyków o szkodliwym działaniu na środowisko przyrodnicze. Alternatywą dla chemicznych pestycydów mogą być preparaty naturalne.
EN
In general, the pesticides mean as the chemical compounds with a broad spectrum of biocidal activity against pests and the economic harmful organisms. Unfortunately, most of these preparations, especially xenobiotics, can influence negatively on the environment and ecosystems. An alternative method control of pests may be used the natural preparations such as biological or mineral.
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tom 49
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nr 2
135-140
EN
Effectiveness of 8 bio-rational insecticides, Dipel 2x, BioFly, Agrin, BioGaurd, Spinosad, Neemix, Mectin and Match were tested at recommended dose, half of recommended dose and quarter of recommended dose against 1st, 3rd and 5th instar larvae and the egg masses (24, 48 and 72 h old) of the Egyptian cotton leafworm Spodoptera littoralis (Boisd.) under laboratory conditions. All the bio-rational insecticides provided higher mortality in the first instar larvae comparing to the third and fifth instar larvae, although Match, Mectin and Spinosad showed also excellent efficacy against third larval stage of S. littoralis at all tested concentrations. Also, Match showed 100% mortality of fifth instar larvae at all tested concentrations. The mortality rates of S. littoralis eggs of different ages (24, 48 and 72 h old) when dipped in recommended dose of each insecticide diluted in water were investigated. At recommended dose of all insecticides eggs of different ages were highly affected and the reductions of hatchability were 83.4, 85.0 and 71.7%, respectively in Spinosad compared to the control. In general, eggs 48 and 72 h old were less sensitive than younger eggs 24 h old. The latent effect of bio-rational insecticides on egg hatchability of S. littoralis was observed only in Match and Neemix with the average being 55.0% and 51.6% respectively. Our results suggest that Match, Mectin and Spinosad are potentially effective compounds for control of S. littoralis.
PL
W warunkach laboratoryjnych badano wpływ ośmiu bioinsektycydów: Dipel 2x, BioFly, Agrin, BioGaurd, Spinosad, Neemix, Mectin i Match zastosowanych w zalecanej dawce, połowie oraz jednej czwartej zalecanej dawki na stadia larwalne 1, 2 i 5 oraz złoża jaj (24, 48 i 72 godzinne) egipskiej sówki bawełnówki Spodoptera littoralis. Wszystkie testowane bioinsektycydy powodowały wyższą śmiertelność pierwszego stadium larwalnego w porównaniu do pozostałych stadiów 3 i 5. Preparaty Match, Mectin i Spinosad wykazały bardzo wysoką skuteczność w stosunku do trzeciego stadium S. littoralis przy zastosowaniu wszystkich badanych koncentracji, a preparat Match powodował 100% śmiertelności piątego stadium larwalnego. Jednocześnie badano wpływ bioinsektycydów na rozwój jaj (24-, 48- i 72-godzinnych) S. littoralis. Złoża jaj zanurzano w wodnym roztworze każdego z testowanych preparatów uwzględniając zalecaną dawkę. Wszystkie testowane preparaty okazały się wysoce skuteczne i ograniczały zdolności jaj do wylęgu larw. W przypadku bioinsektycydu Match skuteczność była najbardziej widoczna i wynosiła odpowiednio dla poszczególnych grup wiekowych jaj 83.4, 85.0 i 71.7% w porównaniu do kontroli. Na ogół jaja 48- i 72-godzinne były mniej wrażliwe na działanie preparatów niż młodsze 24-godzinne jaja. Tylko w przypadku dwóch bioinsektycydów Match i Neemix stwierdzono długotrwały wpływ na rozwój jaj S. littoralis, a średnie wielkości procentowe wynosiły odpowiednio 55.0 i 51.6%. Wyniki przedstawionych badań dowodzą, że biopreparaty Match, Mectin i Spinosad charakteryzują się wysoką skutecznością zwalczania S. littoralis.
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nr 1
EN
Environmental toxicants such as insecticides are able to provoke epigenetic alterations which can be inherited to future generations. The aim of the current study was to assess whether the insecticide Trebon 10F (containing the active ingredient etofenprox) causes multigenerational and/or transgenerational effects on the egg traits of the collembolan Folsomia candida. The parent generation was kept in soil treated with three concentrations of the insecticide. The hatched offspring from each treatment were divided into two groups and were treated in the same manner as their parents (multigenerational effect), or remained untreated (transgenerational effect). The parents reacted with smaller eggs to the insecticide in a concentration dependent manner. Both multigenerational and transgenerational effects were detected in the offspring generations. While neither the number of eggs nor the ratios of the egg diameters (shortest/longest diameter) changed, the egg size increased as the Trebon 10F concentration increased. This is an indication that parents living under insecticide stress transmit this information to their offspring, who react with higher quality egg production. Such alterations in egg traits may have important consequences on the F. candida population dynamic.
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