Wyniki wyszukiwania - Biblioteka Nauki

1

Uptake pathway of lipid vesicles in Tetrahymena pyriformis

100%

Fabczak H.

Acta Protozoologica

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1987

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tom 26

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nr 2

2

The inhibitory effect of ellagic acid on genotoxicity and mutagenicity induced by different doses of benzo[a]pyrene

100%

Gorski T. , Gorecka D. , Sikora M.

Polish Journal of Environmental Studies

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1997

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tom 06

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nr 1

EN

Ellagic acid (EA), known as a naturally occurring plant phenol, has shown a significant influence on genotoxicity and mutagenicity induced by benzo(a)pyrene (B(a)P) in experiments with sister chromatid exchange (SCE) and Ames Salmonella typhimurium revertants. The maximum inhibitory effect of EA has been detected at low levels of B(a)P doses administered intraperitoneal to mice. The frequency of SCE per chromosome in bone marrow cells induced by B(a)P has been greatly lowered by EA at lower B(a)P doses than 0.5 mg/kg body weight of animals. The number of Ames typhimurium revertants has been reduced in the highest degree at about 10 μg of B(a)P per plate.

3

The effect of trans-2-hexenal and trans-2-nonenal on the mycelium growth of Phoma narcissi in vitro

86%

Saniewska A. , Saniewski M.

Roczniki Akademii Rolniczej w Poznaniu. Ogrodnictwo

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2007

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tom 41

189-193

4

Inhibitory effect of antibiotics on the growth of heterotrophic bacteria inhabiting marine beach

86%

Perlinski P. , Mudryk Z.

Baltic Coastal Zone. Journal of Ecology and Protection of the Coastline

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2009

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tom 13 part II

EN

The objective of the present study was to evaluate heterotrophic bacteria capable of growth in the presence of different antibiotics and their mixture in such dynamic ecosystem as marine beach. Mixture antibiotics had the strongest inhibitory effect on the growth of bacteria inhabiting sand of studied beach. Culturable bacteria were more resistant to ampicillin than to novobiocin and tetracycline. Inhibitory influence antibiotics on growth bacteria inhabiting studied beach were in the following order: mixture antibiotics > novobiocin > tetracycline > ampicillin.

PL

W pracy przedstawiono wyniki badań dotyczących określenia hamującego wpływu rożnych antybiotyków i ich mieszaniny na wzrost heterotroficznych bakterii zasiedlających piasek plaży morskiej zlokalizowanej na terenie Słowińskiego Parku Narodowego w rejonie Czołpina. Proby piasku na tej plaży pobierano w profilu horyzontalnym z czterech stanowisk (morze, strefa brzegowa, środkowa część plaży, wydma), a w profilu wertykalnym na każdym stanowisku z dwóch (0-5 cm, 10-15 cm) głębokości. Badania te wykazały, że mieszanina antybiotyków w podłożu hodowlanym wywierała bardziej hamujący wpływ na wzrost bakterii zasiedlających badaną plażę niż pojedyncze antybiotyki. Wśród testowanych antybiotyków neomycyna i tetracyklina wykazywały znacznie większy niż ampicylina hamujący wpływ na wzrost bakterii. Wykazano, że hamujący wpływ antybiotyków i ich mieszaniny na wzrost bakterii zasiedlających powierzchniowe i podpowierzchniowe warstwy piasku był podobny. Stwierdzono, że testowane antybiotyki i ich mieszanina miały wpływ na wzrost chromogennych i achromogennych bakterii zasiedlających piasek badanej plaży morskiej.

5

Inhibitory effects of different medicinal plants on Candida albicans growth

86%

Tambur Z. , Cenic Milosevic D. , Mileusnic I. , Doder R. , Marjanovic M. , Miljkovic Selimovic B. , Kulisic Z. , Opacic D.

Medycyna Weterynaryjna

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2018

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tom 74

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nr 07

EN

The objective of this study was to evaluate the in vitro antifungal properties of ethanol extracts and essential oils of medicinal plants from Serbia against Candida albicans (C. albicans) ATCC 10231. Ethanol extracts of fifteen plants were investigated, and their effects were compared with those of three different essential oils. The sensitivity of C. albicans to all plants was tested by the agar dilution method. The assay plates were estimated to contain 300, 150, 75, and 37.5 µg/ml of active extracts and 100, 50, 25, and 12.5 µg/ml of active essential oils. Inocula were applied to agar surfaces, giving approximately 106 cfu/ml of C. albicans. No inhibitory effects were observed for ethanol extracts of Hypericum perforatum and Salvia officinalis (MIC > 300 µg/ml). The most effective were the ethanol extract of Aesculus hippocastanum (MIC = 37.5 µg/ml) and the essential oil of Satureja kitaibelii (MIC = 12.5 µg/ml). Other plants showed MIC from 25 to 300 µg/ml. As far as we know, the inhibitory effects of these medicinal plants against the reference strain of C. albicans have not been commonly investigated in our country. Although the essential oil of Satureja kitaibelii shows strong activity against C. albicans, these results need clinical evaluation.

6

Clinical relevance of the inhibitory effect of green tea catechins [GTCs] on prostate cancer progression in combination with molecular profiling of catechin-resistant tumors: an integrated view

86%

Bettuzzi S. , Rizzi F. , Belloni L.

Polish Journal of Veterinary Sciences

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2007

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tom 10

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nr 1

EN

Prostate cancer (CaP) is a fast-growing health and social problem already representing the second leading cause of cancer-related death among men in Western countries. Lifestyle-related factors and diet are major contributors for CaP promotion. Because of unfavourable prognosis of extra-prostatic CaP, prevention is considered the best approach to fight it at present time. Green Tea Catechins (GTCs) were proven effective at inhibiting cancer growth in several laboratory studies. We recently performed a pilot clinical trial in HG-PIN subjects showing that only 1/30 tumour was diagnosed in subjects treated for 1 year with 600 mg/die GTCs, while 9/30 cancers were found in placebo-treated men. CaP is an elusive disease, whose biological behaviour is difficult to predict. We have recently described and validated a RT-qPCR method based on a 8-genes signature that significantly discriminated benign tissue from CaP in both humans and TRAMP mice spontaneously developing CaP. In the animal model, also GTCs-resistant CaP was significantly discriminated from GTCs-sensitive CaP, i.e. responding to GTCs administration. Preliminary experiments in our laboratory have shown that this method can be successfully applied to a single tissue needle biopsy specimen in humans. The combination of these results may be of particular significance on the field. In fact, GTCs treatment for men at high risk of CaP as first line prevention therapy in combination with the 8-genes signature profiling in tissue needle biopsies for real time monitoring of patient's response might importantly change, in the near future, the clinical managing of this highly diffuse malignancy.

7

Inhibitory effect of Ca2+ on ATP-mediated stimulation of NPR-A-coupled guanylyl cyclase in renal glomeruli from spontaneously hypertensive and normotensive rats

86%

Woodard G. E. , Zhao J. , Rosado J. A.

Journal of Physiology and Pharmacology

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2006

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tom 57

|

nr 3

EN

Atrial natriuretic peptide (ANP) regulates blood pressure mainly through the occupation of the guanylyl cyclase-coupled receptor NPR-A, which requires ATP interaction for maximal activation. This study investigates the effect of extracellular Ca2+ on ATP-mediated regulation of NPR-A-coupled guanylyl cyclase activity in glomerular membranes from Wistar Kyoto (WKY) and spontaneously hypertensive rats (SHR). ATP induced a significant increase in basal and ANP1-28-stimulated guanylyl cyclase activity that was greater in SHR than in WKY. Extracellular Ca2+ inhibited ATP-stimulated guanylyl cyclase activity in a concentration-dependent manner, but did not modify basal and ANP1-28-stimulated guanylyl cyclase activity. In the presence of ATP, NPR-A showed higher affinity for ANP1-28 and lower Bmax. Ca2+ did not modify NPR-A-ANP1-28 binding properties. The different effects of extracellular Ca2+ on ANP1-28- or ATP-mediated guanylyl cyclase activation suggest that these events are differentially regulated. Addition of extracellular Ca2+ induced similar effects in hypertensive and normotensive rats, suggesting that it is not responsible for the elevated cGMP production observed in SHR.

8

Inhibitory effect of complement inactivation and epigallocatechin gallate on the human serum ability to induce cutaneous neovascular reaction in mice

86%

Skopinski P. , Skopinska-Rozewska E. , Sommer E. , Chorostowska-Wynimko J. , Demkow U. , Rogala E. , Filewska M.

Bulletin of the Veterinary Institute in Puławy

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2004

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tom 48

|

nr 4

9

Coenzyme Q releases the inhibitory effect of free fatty acids on mitochondrial glycerophosphate dehydrogenase

86%

Rauchova H. , Drahota Z. , Rauch P. , Fato R. , Lenaz G.

Acta Biochimica Polonica

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2003

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tom 50

|

nr 2

EN

Data presented in this paper show that the size of the endogenous coenzyme Q (CoQ) pool is not a limiting factor in the activation of mitochondrial glyceropho- sphate-dependent respiration by exogenous CoQ3, since successive additions of succinate and NADH to brown adipose tissue mitochondria further increase the rate of oxygen uptake. Because the inhibition of glycerophosphate-dependent respiration by oleate was eliminated by added CoQ3, our data indicate that the activating effect of CoQ3 is related to the release of the inhibitory effect of endogenous free fatty acids (FFA). Both the inhibitory effect of FFA and the activating effect of CoQ3 could be demonstrated only for glycerophosphate-dependent respiration, while succinate- or NADH-dependent respiration was not affected. The presented data suggest differences between mitochondrial glycerophosphate dehydrogenase and succinate or NADH dehydrogenases in the transfer of reducing equivalents to the CoQ pool.

10

The inhibitory effect of copper ions on lymphocyte Kv1.3 potassium channels

86%

Teisseyre A. , Mozrzymas J. W.

Journal of Physiology and Pharmacology

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2006

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tom 57

|

nr 2

EN

We applied the whole-cell patch-clamp technique to study the inhibitory effect of copper ions (Cu) on the activity of Kv1.3 channels expressed in human lymphocytes. Application of Cu reversibly inhibited the currents to about 10% of the control value in a concentration-dependent manner with the half blocking concentration of 5.28±0.5 µM and the Hill's coefficient of 3.83±0.18. The inhibitory effect was saturated at 10 µM concentration. The inhibition was time-dependent and it was correlated in time with a significant slowing of the current activation rate. In contrast the voltage dependence of activation was not changed by Cu as well as the inativation kinetics. The inhibitory effect of Cu was voltage-independent. It was also unaffected by changing the extracellular pH in the range from 6.4 to 8.4, raising the extracellular potassium concentration to 150 mM and by changing the holding potential from -90 to -60 mV. The inhibitiory effect of Cu was not changed in the presence of an equivalent concentration of Zn. Altogether, obtained data suggest that Cu inhibits Kv1.3 channels by a different mechanism than Zn and that Cu and Zn act on different binding sites. The inhibitory effect of Cu was probably due to a specific binding of Cu on binding sites on the channels. Possible physiological significance of the Cu-induced inhibition of Kv1.3 channels is discussed.

11

Antifungal activity of pathogenesis - related [PR] proteins of cucumber infected TNV

86%

Kollerova E. , Srobarova A.

Hodowla Roślin, Aklimatyzacja i Nasiennictwo

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1993

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tom 37

|

nr 4

12

Efficacy of coupling gamma irradiation with calcium chloride and lemongrass oil in maintaining guava fruit quality and inhibiting fungal growth during cold storage

72%

Hassanein R. A. , Salem E. A. , Zahran A. A.

Folia Horticulturae

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2018

|

tom 30

|

nr 1

13

Influence of 2,5-dichloro-1,4-benzoquinone on jack bean urease activity. Inhibitory effect, total reducing capacity and DPPH radical scavenging activity

72%

Kot M. , Olech Z.

Acta Biochimica Polonica

|

2011

|

tom 58

|

nr 4

EN

Inhibition of jack bean activity by 2,5-dichloro-1,4-benzoquinone (DCBQ) was studied in phosphate buffer, pH 7.0. It was found that DCBQ acted as a strong, time and concentration dependent inactivator of urease. Under the experimental conditions obeyed the terms of pseudo-first-order reaction, urease was totally inactivated. Application of Wilson-Kitz method proved that the urease-DCBQ interaction followed a simple bimolecular process and the presence of intermediate complex was undetectable. The determined second order rate constant of the inactivation was 0.053 (μM min)-1. Thiols such as l-cysteine, glutathione and dithiothreitol (DTT) protected urease from inhibition by DCBQ but DCBQ-modified urease did not regain its activity after DTT application. The thiol protective studies indicated an essential role of urease thiol(s) in the inhibition. The irreversibility of the inactivation showed that the process was a result of a direct modification of urease thiol(s) by DCBQ (DCBQ chlorine(s) substitution). The decomposition of DCBQ in aqueous solution at natural light exposure was monitored by visible spectrophotometry, determination of the total reducing capacity (Folin-Ciocalteu method) and DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging ability. The DCBQ conversion resulted in a decrease of the inhibition power and was well correlated with the increase of the total reducing capacity and DPPH scavenging ability. These findings were attributed to DCBQ transformation by photolysis and the hydrolysis effect was found to be negligible.

14

NO-releasing aspirin exerts stronger growth inhibitory effect on Barrett's adenocarcinoma cells than traditional aspirin

72%

Konturek P. C. , Kania J. , Burnat G. , Hahn E. G.

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tom 57

|

nr 12

15-24

EN

Expression of cyclooxygenase-2 (COX-2) is involved in the chronic inflammation-related development of Barrett’s adenocarcinoma and the use of selective COX-2 inhibitors (coxibs) might provide new chemoprevention strategy for Barrett’s adenocarcinoma (BA). Despite an excellent gastrointestinal (GI) safety profile of coxibs, their use is limited because of the possible cardiovascular complications. The coupling of NSAIDs with a NO-donating moiety has led to the birth of a new class of anti-inflammatory drugs, called the COX-inhibiting nitric oxide donators (CINODs). The member of this group, NO-aspirin (NO-ASA) retains the anti-inflammatory properties of traditional aspirin (ASA), but the release of NO accounts for anti-thromboembolic effect and better GI safety profile. The role of NO-ASA in the prevention of Barrett’s adenocarcinoma (BA) has not been studied so far. Therefore, the aim of the present study was: 1) to analyse the expression of COX-2 in the biopsies obtained from BE; 2) to compare the effect of NO-ASA with that of ASA on proliferation rate in Barrett’s adenocarcinoma cell line (OE-33 cells); 3) to determine the effect of both compounds on the apoptosis rate using FACS analysis and expression of 32-kDa procaspase-3 and active proapoptotic 20-kDa caspase-3 in OE-33 cell line. The expression of COX-2 was assessed in biopsies obtained from the Barrett’s mucosa and normal squamous epithelial esophageal mucosa from 20 BE patients by RT-PCR and Western blot analysis, respectively. The BA cell line (OE-33) was incubated with NO-ASA or ASA (10-1000µM). The cell proliferation and apoptosis rate was measured by BrdU and FACS-analysis, respectively. The expression of caspase-3 (active and inactive form) was analyzed by Western blot. In Barrett’s mucosa a significant up-regulation of COX-2 was observed. Compared with traditional ASA, NO-ASA caused a significantly stronger induction of apoptosis (dose-dependently). Inhibition of cell proliferation in OE-33 cells observed under NO-ASA treatment was due to the apoptosis induction. The increase in apoptotic rate was accompanied by the upregulation of active 20-kDa caspase-3. At the highest concentration (1000µM), a necrotic death of OE-33 cells was observed under NO-ASA treatment. We conclude that: NO-ASA caused induction of apoptosis in BA cell line and slight growth inhibition. These results indicate that this compound may represent a promising chemopreventive agent for Barrett’s adenocarcinoma.

15

In silico assessment of the inhibitory effect of four flavonoids (chrysin, naringin, quercetin, kaempferol) on tyrosinase activity using the MD simulation approach

72%

Farasat A. , Ghorbani M. , Gheibi N. , Shariatifar H.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2020

|

tom 101

|

nr 3

16

Inhibitory effects of antagonistic bacteria inhabiting the rhizosphere of the sugarbeet plants, on Cercospora beticola Sacc., the causal agent of Cercospora leaf spot disease on sugarbeet

72%

Arzanlou M. , Mousavi S. , Bakhshi M. , Khakvar R. , Bandehagh A.

Journal of Plant Protection Research

|

2016

|

tom 56

|

nr 1

EN

In the present study, the antagonistic capability of bacterial agents inhabiting the rhizosphere of sugarbeet plants were evaluated against Cercospora beticola Sacc. under laboratory and greenhouse conditions. After preliminary screening using the dual culture method, 14 strains with higher antagonistic capability were selected for further inhibitory assays against C. beticola. Bacterial strains were identified based on the sequence data of the small subunit-rDNA (SSU-rDNA) gene. Based on the SSU sequence data, the identity of bacterial strains were determined as Bacillus (10 strains: RB1, RB2, RB3, RB4, RB5, RB6, RB7, RB8, RB9, RB10), Paenibacillus (two strains: RP1, RP2), Enterobacter (one strain: RE), and Pseudomonas (one strain: RPs). The results obtained in this study showed that in all of the assays (dual culture, volatile and non-volatile metabolites) bacterial antagonists significantly inhibited the growth of C. beticola compared to the control. Bacillus (RB2) showed the highest inhibition rate on C. beticola in all of the assays. Based on the results of the laboratory assays, three bacterial strains RB2 (Bacillus), RPs (Pseudomonas), and RE (Paenibacillus) were selected for greenhouse assays. The experiment was designed based on a completely randomised design (CRD) with the application of antagonists prior to, simultaneously, and after inoculation with C. beticola on sugarbeet leaves. The reduction in disease severity was evaluated seven days after inoculation. The results of greenhouse assays were consistent with the results of laboratory studies. The obtained results showed that bacterial antagonists significantly reduced the disease severity when compared to the control.

17

Study and preparation of an environmentally friendly corn seed coating agent

72%

Zeng D. , Mei X. , Wu J.

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tom 50

|

nr 2

210-214

EN

Head smut of corn is caused by the fungus Sphacelotheca reiliana and occurs in northeast China and in regions of a similar climate. Yield losses due to the disease are variable and directly depend on the severity of the disease. The objective of this study was to produce a coating technology to protect corn from head smut and to avoid environmental pollution. Based on its excellent properties of high efficiency, nonpollution and nontoxicity, a novel seed coating agent was prepared with modified chitosan as the main material and trace elements and fertilizer as the auxiliary material. Compared with the conventional toxic seed coating agent, the novel seed coating agent protected the seeds and provided excellent control of head smut and increased yield by 11.6 to 14.6%, while the cost of seed coating agent decreased by 32.4%. Our findings indicate that the application of chitosan in seed coating technology had a remarkable effect on the resistance to head smut of corn and yield enhancement.

PL

Grzyb Sphacelotheca reiliana, sprawca głowni pylącej kukurydzy, występuje w północno-wschodnich Chinach oraz na terenach o podobnym klimacie. Straty plonu są zmienne, choć bezpośrednio zależą od nasilenia choroby. Przedmiotem badań było opracowanie technologii zaprawiania nasion przeciwko głowni kukurydzy. Bazując na doskonałych właściwościach chitozanu, jego wysokiej efektywności, nie zanieczyszczaniu środowiska oraz braku toksyczności, przygotowano nową zaprawę ze zmodyfikowanym chitozanem jako składnikiem podstawowym oraz śladowymi pierwiastkami i nawozem, pełniącymi rolę pomocniczą. W porównaniu do konwencjonalnej, toksycznej zaprawy, nowa zapewniała doskonałe zwalczanie głowni kukurydzy i powodowała wzrost plonu od 11,6 do 14,6%, podczas gdy koszt zaprawy był mniejszy o 32,4%. Wykorzystanie chitozanu w technologii zaprawiania nasion podnosi odporność kukurydzy na głownię oraz wpływa na wzrost plonu.

18

Silver thiosulphate counteracts of the inhibitory effect of ethephon on tulip stem elongation induced by auxin

72%

Saniewski M. , Kawa L.

Bulletin of the Polish Academy of Sciences. Biological Sciences

|

1988

|

tom 36

|

nr 10-12

19

In vitro alpha-glucosidase and alpha-amylase enzyme inhibitory effects of Andrographis paniculata extract and andrographolide

72%

Subramanian R. , Asmawi M. Z. , Sadikun A.

Acta Biochimica Polonica

|

2008

|

tom 55

|

nr 2

391-398

EN

There has been an enormous interest in the development of alternative medicines for type 2 diabetes, specifically screening for phytochemicals with the ability to delay or prevent glucose absorption. The goal of the present study was to provide in vitroevidence for potential inhibition of α-glucosidase and α-amylase enzymes, followed by a confirmatory in vivostudy on rats to generate a stronger biochemical rationale for further studies on the ethanolic extract of Andrographis paniculata and andrographolide. The extract showed appreciable α-glucosidase inhibitory effect in a concentration-dependent manner (IC50=17.2±0.15 mg/ml) and a weak α-amylase inhibitory activity (IC50=50.9±0.17 mg/ml). Andrographolide demonstrated a similar (IC50=11.0±0.28 mg/ml) α-glucosidase and α-amylase inhibitory activity (IC50=11.3±0.29 mg/ml). The positive in vitroenzyme inhibition tests paved way for confirmatory in vivostudies. The in vivostudies demonstrated that A. Paniculata extract significantly (P<0.05) reduced peak blood glucose and area under curve in diabetic rats when challenged with oral administration of starch and sucrose. Further, andrographolide also caused a significant (P<0.05) reduction in peak blood glucose and area under the curve in diabetic rats. Hence α-glucosidase inhibition may possibly be one of the mechanisms for the A. paniculataextract to exert antidiabetic activity and indicates that AP extract can be considered as a potential candidate for the management of type 2 diabetes mellitus.

20

Inhibitory effect of antipsychotic drugs on the CON A- and LPS- induced proliferative activity of mouse splenocytes: a possible mechanism of action

72%

Basta-Kaim A. , Budziszewska B. , Jagla G. , Nowak W. , Kubera M. , Lason W.

Journal of Physiology and Pharmacology

|

2006

|

tom 57

|

nr 2

EN

Antipsychotic drugs are widely used to alleviate a number of psychic disorders and have been found to modulate some immune parameters, but the molecular mechanism of their action on the proliferative activity has been poorly recognized. In the present study, we investigated effects of various antipsychotics on the proliferative activity of lymphocytes stimulated by concanavalin A (Con A) and lipopolysaccharide (LPS). Chlorpromazine (3x10-6 - 10-4M) showed the most potent effect in inhibiting 3H-thymidine incorporation into C57BL/6 mouse spleen cells stimulated by Con A and LPS. Treatment of the cells with thioridazine (10-5 - 10-4M), promazine (10-5 - 10-4M), haloperidol (10-5 - 10-4M), risperidone (10-5 - 10-4M), raclopride (3x10-5 - 10-4M), remoxipride (3x10-5 - 10-4M) and clozapine ( 3x10-5 - 10-4M), but not with sulpiride (10-7 - 10-4M), suppressed proliferative activity of splenocytes after Con A stimulation. On the other hand, LPS-induced proliferation of splenocytes was inhibited by clozapine, promazine, thioridazine and haloperidol, but not by risperidone, remoxipride, sulpiride and raclopride. In the next part of the study, the influence of some kinase modulators on chlorpromazine- and clozapine-evoked inhibition of the proliferative activity of splenocytes was determined. Wortmannin, a selective phosphatidylinositol 3-kinase (PI3-K) inhibitor, blocked chlorpromazine and clozapine inhibitory effect on the mitogen-stimulated splenocyte proliferation. The involvement of PI 3-K /protein kinase B (PKB, Akt) pathway was confirmed by the results of the Western blot study, which showed that both drugs increased the level of active phospho-Ser-473 Akt, without changing the total Akt level, and decreased the level of active, nonphosphorylated glycogen synthase kinase-3 (GSK-3ß). Additionally, we have found that chlorpromazine action was also attenuated by a selective p-38-MAPK inhibitor, while clozapine effect was suppressed by a protein kinase C (PKC) activator. The obtained results indicated that atypical antipsychotic drugs markedly inhibited the proliferative activity of splenocytes only after ConA stimulation. Inhibition of the proliferative capability of splenocytes by chlorpromazine and clozapine resulted mainly from the activation of PI3-K/Akt pathway.