Wyniki wyszukiwania - Biblioteka Nauki

1

Experimental model for studying effectiveness of treatment and prophylaxis procedures on the beginning and developing of purulent-inflammatory processes

100%

Pohodenko-Chudakova I. O. , Kazakova Y. M.

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2004

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tom R. 7, nr 37

10-11

2

Haemoglobin scavenger receptor: function in relation to disease

60%

Zuwala-Jagiello J.

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tom 53

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nr 2

EN

Highly efficient systems remove the toxic and proinflammatory haemoglobin from the circulation and local sites of tissue damage. Macrophages are major haemoglobin-clearing cells; CD163 was recently recognized as the specific haemoglobin scavenger receptor (HbSR). It is tightly involved in both physiological as well as pathophysiological processes, such as cytoprotection and inflammation. Haemoglobin functions as a double-edged sword. In moderate quantities and bound to haptoglobin, it forms a ligand for haemoglobin scavenger receptor CD163/HbSR, but when unleashed in large amounts, it can become toxic by mediating oxidative stress and inflammation. CD163/HbSR plays a crucial role in the control of inflammatory processes, probably in part through its effects on both ferritin induction and subsequent induction of antiinflammatory pathways through interleukin-10 and haem oxygenase. Besides the observation that the haemoglobin scavenger receptor provides a promising target for new treatment possibilities, it offers a novel view on the aetiology of diverse physiological as well as pathophysiological processes. In addition, monocyte CD163/HbSR and soluble CD163/HbSR are potential diagnostic tools in a variety of disease states, such as inflammation, atherosclerosis, transplant rejection, and carcinoma.

3

Neopterin in patients with chronic kidney disease and patients with coronary artery disease

51%

Formanowicz D.

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nr 1

4

Evaluation of the memory CD4+ and CD8+ T cells homeostasis during chronic venous disease of lower limbs

51%

Ojdana D. , Safiejko K. , Milewski R. , Sacha P. , Wieczorek P. , Lipska A. , Radziwon P. , Dadan J. , Tryniszewska E.

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nr 3

5

Proliferation activity and apoptosis in granulation tissue and cholesteatoma in middle ear reoperations

51%

Durko M. , Kaczmarczyk D.

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nr 1

EN

The aim of the study was to find out the potential prognostic value of proliferation activity and apoptosis in cholesteatoma and granulation tissue removed during middle ear reoperation in recurrent middle ear inflammation. Granulation tissues and recurrent cholesteatoma were analysed after being surgically removed from the middle ear in a group of 25 patients qualified for middle ear reoperation procedure. Paraffin sections were stained with haematoxylin and eosin according to Mallory’s method. Immunohistochemical reaction Anti-PCNA was performed. Apoptosis was evaluated using the TUNEL method. The percentage of PCNA-positive cells was 42–95% in the matrix of the cholesteatoma and 29–81% in the perimatrix. In the granulation tissue it was 35–75%. The percentage of apoptotic cells was 12–73% in the matrix of the cholesteatoma, 5–72% in the perimatrix and 1–65 % in the granulation tissue. The prognostic value of the parameters studied in the recurrent middle ear inflammatory process is questionable, probably due to the small number of cases under examination.

6

A role of hydrogen peroxide producing commensal bacteria present in colon of adolescents with inflammatory bowel disease in perpetuation of the inflammatory process

41%

Strus M. , Gosiewski T. , Fyderek K. , Wedrychowicz A. , Kowalska-Duplaga K. , Kochan P. , Adamski P. , Heczko P. B.

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2009

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tom 60

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nr 6

7

Collagen type II modification by hypochlorite

41%

Olszowski S. , Mak P. , Olszowska E. , Marcinkiewicz J.

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nr 2

EN

Oxidation of proteins is a common phenomenon in the inflammatory process mediated by highly reactive agents such as hypochlorite (HOCl/OCl-) produced by activated neutrophils. For instance, in rheumatoid arthritis hypochlorite plays an important role in joint destruction. One of the major targets for HOCl/OCl- is collagen type II (CII) — the primary cartilage protein. In our study, HOCl/OCl- mediated collagen II modifications were tested using various methods: circular dichroism (CD), HPLC, ELISA, dynamic light scattering (DLS), fluorimetry and spectrophotometry. It was shown that hypochlorite action causes deamination with consecutive carbonyl group formation and transformation of tyrosine residues to dichlorotyrosine. Moreover, it was shown that ammonium chloramine (NH^Cl) formed in the reaction mixture reacts with CII. However, in this case the yield of carbonyl groups and dichlorotyrosine is lower than that observed for HOCl/OCl- by 50%. CD data revealed that collagen II exists as a random coil in the samples and that chlorination is followed by CII fragmentation. In the range of low HOCl/OCl- concentrations (up to 1 mM) 10-90 kDa peptides are predominant whereas massive production of shorter peptides was observed for high (5 mM) hypochlorite concentration. DLS measurements showed that chlorina- tion with HOCl/OCl- decreases the radius of collagen II aggregates from 30 to 6.8 nm. Taking into account the fact that chlorinated collagen is partially degraded, the DLS results suggest that smaller micelles are formed of the 10-90 kDa peptide fraction. Moreover, collagen chlorination results in epitope modification which affects CII recognition by anti-CII antibodies. Finally, since in the synovial fluid the plausible hypochlorite concentration is smaller than that used in the model the change of size of molecular aggregates seems to be the best marker of hypochlorite-mediated collagen oxidation.

8

Estimation of IL-1beta and IL-8 in gastric juice and blood serum in children with Helicobacter pylori

41%

Bak-Romaniszyn L. , Planeta-Malecka I. , Czkwianianc E. , Zeman K. , Pokoca L. , Kulig A.

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tom 52

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nr 2