Wyniki wyszukiwania - Biblioteka Nauki

1

'Bezobjawowe' wirusy drzew owocowych

100%

Cieslinska M.

Owoce, Warzywa, Kwiaty

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2002

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nr 12

26-27

2

Ocena wystepowania latentnych zakazen herpeswirusowych u lisow hodowlanych

100%

Rzezutka A. , Jakubczak A. , Mizak B.

Medycyna Weterynaryjna

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2008

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tom 64

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nr 05

717-719

EN

Abortions, neonatal deaths, and reproductive failures in a variety of animal species are caused by many pathogens, including alphaherpesviruses. Herpesviruses can persist in the form of a latent infection in a variety of tissues of the infected animals. In addition, the reactivation of the latent virus infection, especially in pregnant bitches, can lead to abortions or stillbirths. These problems are frequently reported on fox farms in Poland. The aim of this study was to establish if canine herpesvirus (CHV) can be considered as a viral pathogen responsible for the reproductive losses on fox farms. In the authors’ study, 114 samples of internal organs of dead puppies and adult animals eliminated from breeding have been examined using polymerase chain reaction (PCR). None of the tested samples contained herpesviral DNA, the presence of which could confirm the existence of latent infections in foxes.

3

Zastosowanie real-time PCR w wykrywaniu zarażen subklinicznych Babesia canis u psów - ocena wpływu różnych metod izolacji DNA na czułość PCR

80%

Adaszek L. , Dziegiel B. , Gorna M. , Garbal M. , Wernicka-Furmaga R. , Winiarczyk S.

Medycyna Weterynaryjna

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2012

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tom 68

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nr 06

EN

The aim of this study was to use the real time polymerase chain reaction in the detection of Babesia canis subclinical infestations in dogs and to compare the different DNA isolation methods on PCR sensitivity. The study included 6 dogs with suspected subclinical babesiosis. DNA for real time polymerase chain reaction were isolated by the phenol method as well as by Micro AX Gravity (A & A Biotechnology, Gdynia, Poland) and Blood mini (A & A Biotechnology, Gdynia, Poland) commercial kits. In the blood of all six specimens PCR demonstrated the presence of Babesia canis DNA. The most efficient proved to be a reaction to which the genetic material was isolated by the phenol method. The amount of total DNA obtained in this way, determined spectrometrically, ranged 43.7-54.3 ng/µl. Ct value in real-time PCR for DNA samples isolated in this manner was the lowest in comparison with other isolation methods, and averaged 22.5. Similar results were obtained when DNA was isolated from the blood with the Micro AX Gravity kit, while the least efficient was the Blood Mini Kit (amount of total DNA, depending on the sample was 14.0-25.1 ng/µl, amplification in real time occurred the slowest - average Ct value = 28). Readable sequences were obtained for all PCR products where DNA was isolated using the phenol method or by Micro AX Gravity. In the case of PCR products where DNA was isolated by the Blood Mini Kit, readable sequences were obtained only for 3 out of 6 tested samples. All sequences received in our study of the 18S RNA gene fragment showed a high 99.9-100% homology with the sequence of Babesia canis EU622792 These results confirm the usefulness of the real time PCR in the diagnosis of subclinical canine babesiosis and indicate the need for choosing such a DNA isolation method for this reaction that will guarantee the highest efficiency of amplification.

4

Utajone zakażenie gruźlicą u polskich funkcjonariuszy służby więziennej

80%

Filipek-Czerska A. , Karczewski J. K. , Gładysz I.

Medycyna Pracy

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2021

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tom 72

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nr 4

415-422

EN

Tuberculosis is a chronic infectious disease caused by acid-fast mycobacteria from the Mycobacterium tuberculosis complex group. The disease spreads when people with pulmonary tuberculosis excrete bacteria when they cough, sneeze, laugh or speak. Prisons are often called reservoirs of patients with tuberculosis, posing a great threat to their staff. Longer working hours in prison and penitentiary facilities aid latent contamination among the prison staff. So far, there have been few works discussing latent tuberculosis infection issues among the prisoners and prison staff.Material and MethodsA total of 84 employees of penitentiary facilities located in the Lublin Voivodship were examined using the QuantiFERON-TB Gold In-Tube (QFT-GIT) test which was approved for use in 2005 by the U.S. Food and Drug Administration as an aid in the diagnosis of Mycobacterium tuberculosis. The material for the research included blood samples collected using the QFT-GIT.ResultsPositive test results of latent tuberculosis infections were found in 16.6% of officers. The highest percentage with positive results of tuberculosis infections was found among the surveyed single women aged 36–57, working in penitentiary facilities. There have been no statistically significant differences between the position of officers and the risk factors increasing the chance of the latent tuberculosis infection. Work experience and contact with inmates were found to have an impact on tuberculosis infection.ConclusionsThe obtained results partially confirm other authors’ observations concerning the influence of the risk factors of latent tuberculosis infections in prisons. Work experience and contact with inmates have an impact on tuberculosis infections among the prison staff in the Lublin Voivodeship. Med Pr. 2021;72(4):415–22

PL

Gruźlica jest chorobą zakaźną wywoływaną przez prątki kwasooporne z grupy Mycobacterium tuberculosis complex. Choroba rozprzestrzenia się, gdy chorzy na gruźlicę płucną wydalają bakterie podczas kaszlu, kichania, śmiania się czy mówienia. Więzienia, które są często nazywane rezerwuarami chorych na gruźlicę, stwarzają duże zagrożenie dla zatrudnionych w nich osób. Dłuższy czas pracy w więzieniu i ośrodkach penitencjarnych sprzyja zakażeniu latentnemu wśród personelu więziennego. Dotychczas opublikowano niewiele prac dotyczących zagadnienia latentnego zakażenia prątkami gruźlicy wśród pracowników zakładów karnych.Materiał i metodyPrzebadano łącznie 84 pracowników zakładów karnych zlokalizowanych w województwie lubelskim. Zastosowano test QuantiFERON-TB Gold In-Tube (QFT-GIT), który w 2005 r. został zatwierdzony do użytku przez amerykańską Agencję ds. Żywności i Leków jako pomoc w diagnozowaniu zakażenia M. tuberculosis. Materiał do badań stanowiły próbki krwi pobrane metodą testu QFT-GIT.WynikiLatentne zakażenie gruźlicą stwierdzono u 16,6% funkcjonariuszy. Największy odsetek wyników dodatnich zaobserwowano wśród badanych funkcjonariuszek z zakładu karnego, w wieku 36–57 lat, stanu cywilnego wolnego. Nie wykazano istotnych statystycznie różnic między stanowiskiem pracy funkcjonariuszy służby więziennej a czynnikami ryzyka zakażenia gruźlicą latentną. Okazało się, że na ryzyko zakażenia gruźlicą wpływają staż pracy i kontakt z osadzonymi.Wnioski Wyniki badań częściowo potwierdzają obserwacje innych autorów dotyczące oddziaływania czynników ryzyka latentnego zakażenia gruźlicą pracowników zakładów karnych. W badanych zakładach karnych staż i kontakt miały wpływ na zakażenie. Med. Pr. 2021;72(4):415–422

5

Wirus pasiastej mozaiki jęczmienia (Barley stripe mosaic virus, BSMV) – utajony patogen jęczmienia

70%

Jezewska M. , Cajza M.

Progress in Plant Protection

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2010

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tom 50

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nr 1

EN

Barley stripe mosaic virus (BSMV) was found in seeds of 24 varieties originated from barley cultivar collections per 40 tested. The virus more frequently occurred in seeds of winter cultivars where it was detected in seeds of 17 varietes per 19 tested while in seeds of spring cultivars it was found only in 7 cultivars per 21 tested. Similar results were obtained in the case of commercial varieties. The infection of seeds of some varieties exceeded 10%. In all cases occurrence of the mild and no symptoms was confirmed. Preliminary studies showed that BSMV caused the decrease of yield by 9–15% and the decrease of mass of 1 000 seeds ranging from 0.5 to 3%. The risk of isolate mutation of a mild BSMV to the isolate causing severe symptoms was observed in spring barley cultivar Annabell.

6

Wpływ chorób wymienia i niepłodności na wydajność mleczną krów w województwie kieleckim

60%

Grus W.

Przegląd Mleczarski

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1965

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tom 14

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nr 09