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EN
Drought is a major limitation for rice production in rainfed ecosystems. Identifying quantitative trait loci (QTLs) linked to drought resistance provides opportunity to breed high yielding rice varieties suitable for drought-prone areas. Although considerable efforts were made in mapping QTLs associated with drought-resistance traits in rice, most of the studies involved indica × japonica crosses and hence, the drought-resistance alleles were contributed mostly by japonica ecotypes. It is desirable to look for genetic variation within indica ecotypes adapted to target environment (TE) as the alleles from japonica ecotype may not be expressed under lowland conditions. A subset of 250 recombinant inbred lines (RILs) of F₈ generation derived from two indica rice lines (IR20 and Nootripathu) with contrasting drought-resistance traits were used to map the QTLs for morpho-physiological and plant production traits under drought stress in the field in TE. A genetic linkage map was constructed using 101 polymorphic PCR-based markers distributed over the 12 chromosomes covering a total length of 1,529 cM in 17 linkage groups with an average distance of 15.1 cM. Composite interval mapping analysis identified 22 QTLs, which individually explained 4.8–32.2% of the phenotypic variation. Consistent QTLs for drought-resistance traits were detected using locally adapted indica ecotypes, which may be useful for rainfed rice improvement.
EN
Four F2 mapping populations derived from crosses between rye inbred lines DS2×RXL10, 541×Ot1-3, S120×S76 and 544×Ot0-20 were used to develop a consensus map of chromosome 6R. Thirteen marker loci that were polymorphic in more than one mapping population constituted the basis for the alignment of the four maps using the JoinMap v. 3.0 software package. The consensus map consists of 104 molecular marker loci including RFLPs, RAPDs, AFLPs, SSRs, ISSRs, SCARs, STSs and isozymes. The average distance between the marker loci is 1.3 cM, and the total map length is 135.5 cM. This consensus map may be used as a source of molecular markers for the rapid development of new maps of chromosome 6R in any mapping population.
EN
9-months tapeworms obtained from 3- 6-specimen populations of the 42nd generation of Hymenolepis diminuta WMS „strain" were compared with tapeworms obtained from quantitatively similar populations deriving from one-specimen infections of H. diminuta WMS il1. In both groups of tapeworms the majority of strobilas had unilateral position of genital pares (PGP), while the remaining ones were characterized by variable PGP. The latter appeared in 41.4% of H. diminuta WMS „strain" tapeworms and in only 24.6% of tapeworms coming from H. diminuta WMS il1. In tapeworms with unilateral as well as with variable PGP deriving from H. diminuta WMS il1, the average number of type 0p3a proglottids (all three testes on the aporal side) was significantly higher than in H. diminuta WMS „strain". It results most probably from suitable selection of mother specimens for successive generations of H. diminuta WMS il1. In both compared groups the growth of the number of PGP changes was correlated positively with the number of type 2p1a proglottids and negatively with the numer of type 1p2a and 0p3a proglottids.
EN
Application of AFLPs linked to pollen fertility restoration and nonperforming genes evaluated in the C394-F2 hybrid was studied using a set of male sterile lines in the sterilising Pampa cytoplasm, several restorers and maintainer lines and, finally, two inbred lines backcrossed into cms-P, cms-R, cms-S and cms-C cytoplasms each. The set of male sterile lines based on the Pampa cytoplasm exhibited gradual variation in their ability to restore pollen fertility (starting from low and closing with high) in crosses with three unrelated restorers. Variations in the AFLPs between the analysed materials were observed, however, no clustering of the lines according to their sterile and fertile phenotypes was observed. The same markers, when applied to the population restorer (cv. Walet) that formed the C394-F2 cross permitted identification of plants with genotypes that could be recognized as restorers.
PL
Badano potomstwo pochodzące ze skrzyżowania 6 linii wsobnych formy dent z 8 testerami. Doświadczenie założono metodą bloków niekompletnych w dwóch powtórzeniach, w dwóch miejscowościach. Wykonano pomiary następujących cech: plonu ziarna z poletka przy 15% wilgotności, procentowej zawartości suchej masy w ziarnie, procentu roślin porażonych głownią, procentu roślin wyległych przed zbiorem oraz odporności na krzewienie. Wykonano analizę wariancji dla ogólnej i swoistej zdolności kombinacyjnej oraz oszacowano efekty GCA i SCA. Stwierdzono zróżnicowanie środowisk pod względem badanych cech. Wykazano istotną interakcję efektów ogólnej zdolności kombinacyjnej dla wszystkich cech za wyjątkiem plonu ziarna. Na uwagę zasługuje linia 1390-19 zwiększająca u potomstwa zawartość suchej masy w ziarnie oraz linia 1417-3 zwiększająca odporność na krzewienie. Istotną interakcję efektów swoistej zdolności kombinacyjnej ze środowiskami stwierdzono dla wszystkich badanych cech. Na wyróżnienie zasługują linie tworzące mieszańca 1390-19 x K324 o dodatnich efektach SCA dla plonu i odporności na krzewienie w obu miejscowościach oraz linia 1560-5 i K324 o dodatnich efektach dla zawartości suchej masy.
EN
Offspring of 6 maternal dent lines crossed with 8 testers were investigated. The experiments were carried out at two locations with two replications using incomplete block design. Data were recorded for grain yield, dry matter content, lodging before harvest, tillering and smutted plants. Analyses of general and specific combining ability were done. There were significant differences between environments for all traits. Interactions GCA and SCA effects with environments were significant for all traits except grain yield. Lines 1390-1; 1417-3 and hybrids 1390-19 x K324 and 1560-5 x K324 were the most valuable.
EN
Some results of first trials within the frames of project "Principles of selection of maize genotypes with low nitrogen fertiliser requirements" are presented in the paper. Experiments were conducted on the rinsed sand in Mits- cherlich's pots and in the fields. Fifty-eight inbred lines of maize from breeding company „Hodowla Roślin Smolice" Ltd. were evaluated at two N level in the substrate: without N and 2.25 g N.pot-1 or with only natural N level in the soil and 150 kg N.ha-1 in field experiment. In studied base of maize inbred lines large genetic variability of the response to reduced N supply in the substrate is described. Yield of evaluated maize lines was not closely related to N Nutrition Index (NNI) in the case of nitrogen deficiency. Some root traits: root volume and root N status seem to be related to maize adaptation to N deficiency in substrate. There are indicated groups of genotypes with similar response on N stress for further, more detailed experiments.
PL
W opracowaniu prezentowane są niektóre wyniki pierwszych eksperymentów prowadzonych w ramach projektu „Podstawy selekcji genotypów kukurydzy o ograniczonych potrzebach nawożenia azotem". Doświadczenia były realizowane w wazonach Mitscherlich'a na wymytym piasku oraz w warunkach polowych. 58 linii wsobnych kukurydzy pochodzących z „Hodowla Roślin Smolice" Sp. z o.o. było ocenianych w dwóch warunkach zawartości azotu w podłożu: bez azotu i 2,25 g N na wazon w doświadczeniu wazonowym lub tylko naturalna zawartość N w glebie i 150 kg N.ha-1 w doświadczeniu polowym. Stwierdzono dużą zmienność genetyczną reakcji kukurydzy na ograniczoną zawartość azotu w podłożu. W warunkach niedoboru azotu, plony kukurydzy nie były ściśle powiązane z indeksem stanu odżywienia azotem (NNI). Niektóre cechy systemu korzeniowego: objętość korzeni i zawartość azotu w korzeniach wydają się być powiązane z adaptacją kukurydzy do warunków niedoboru azotu w podłożu. Dokonano podziału genotypów na skupienia - grupy cechujące się podobną reakcją na stres azotowy, w celu poddania ich dalszym, bardziej szczegółowym badaniom.
EN
The role of ABA in chilling tolerance (CT) of maize seedling has been reinvestigated using a set of new maize inbred lines with defined differences in CT. Under chilling conditions (4°C) the ABA level increased in all lines investigated. This increase was significantly higher in the chilling tolerant lines than in sensitive ones. Between ABA level and extent of chilling injury a significant relationship was observed. ABA level correlated negatively with percent necrotic injury (-0.604**) and positively with qP (0.606**). Thus, the study clearly indicates a relationship between the ABA accumulation under chilling stress and extent of injury in maize according to the hypothesis that CT in maize is related to the ability for the accumulation of ABA under chilling stress conditions.
PL
Badano rolę ABA w odporności na chłód siewek kukurydzy na nowym zestawie linii wsobnych kukurydzy o ustalonych różnicach w odporności na chłód. W warunkach chłodu (4°C) poziom ABA wzrastał u wszystkich badanych genotypów. Wzrost ten był istotnie wyższy u linii odpornych na chłód w porównaniu z wrażliwymi. Między poziomem ABA a stopniem uszkodzeń chłodowych obserwowano istotną korelację. Poziom ABA korelował ujemnie z procentem uszkodzeń nekrotycznych (-0,604**) a pozytywnie z qP (0,606**).
PL
Badania wykonano na 20 liniach wsobnych kukurydzy w Kobierzycach i Smolicach. Oceniono wysokość roślin, długość kolby, liczbę rzędów w kolbie, liczbę ziaren i masę ziarna z kolby. Dla wymienionych cech wyliczono indeksy selekcyjne: „IS” — specyficzny, „IO” — obcy oraz,, IOG" — ogólny. Na podstawie uzyskanych wyników stwierdzono, że indeks wartości własnej (specyficzny) jest najlepszą miarą oceny wartości hodowlanej. Indeks obcy obniża wartość postępu genetycznego. Ze względu na niewielką ilość danych eksperymentalnych przydatność indeksu ogólnego nie mogła być wiarygodnie określona.
EN
Studies were carried out on 20 inbred maize lines at two locations: Kobierzyce and Smolice. The following characters: plant height, ear length, no. of rows/ear, no. of' grains/ear and grain weight/ear were included in the construction of three selection indices: specific (Is), foreign (IO) and general (IOG). The results indicated that specific index was the most efficient. Expected genetic advance was lower when foreign index was used. Adequate evaluation of general index could not be made because of insufficient experimental data.
EN
Common smut in maize, caused by Ustilago maydis, reduces grain yield greatly. Agronomic and chemical approaches to control such diseases are often impractical or ineffective. Resistance breeding could be an efficient approach to minimize the losses caused by common smut. In this study, quantitative trait loci (QTL) for resistance to common smut in maize were identified. In 2005, a recombinant inbred line (RIL) population along with the resistant (Zong 3) and susceptible (87-1) parents were planted in Beijing and Zhengzhou. Significant genotypic variation in resistance to common smut was observed at both locations after artificial inoculation by injecting inoculum into the whorl of plants with a modified hog vaccinator. Basing on a genetic map containing 246 polymorphic SSR markers with an average linkage distance of 9.11 cM, resistance QTL were analysed by composite interval mapping. Six additive-effect QTL associated with resistance to common smut were identified on chromosomes 3 (three QTL), 5 (one QTL) and 8 (two QTL), and explained 3.2% to 12.4% of the phenotypic variation. Among the 6 QTL, 4 showed significant QTL x environment (Q x E) interaction effects, which accounted for 1.2% to 2.5% of the phenotypic variation. Nine pairs of epistatic interactions were also detected, involving 18 loci distributed on all chromosomes except 2, 6 and 10, which contributed 0.8% to 3.0% of the observed phenotypic variation. However, no significant epistasis x environment interactions were detected. In total, additive QTL effects and Q x E interactions explained 38.8% and 8.0% of the phenotypic variation, respectively. Epistatic effects contributed 15% of the phenotypic variation. The results showed that besides the additive QTL, both epistasis and Q x E interactions formed an important genetic basis for the resistance to Ustilago maydis in maize.
PL
Materiał badawczy stanowiły mieszańce wysiane w dwóch doświadczeniach założonych metodą kraty kwadratowej częściowo zrównoważonej. Uzyskano je po skrzyżowaniu dwóch grup linii (po 12) z 4 testerami, będącymi liniami lub mieszańcami pojedynczymi. Oceniono wysokość plonu ziarna, zawartość suchej masy w ziarnie podczas zbioru, porażenie roślin głownią i krzewienie. Oszacowano efekty ogólnej zdolności kombinacyjnej (GCA) i efekty swoistej zdolności kombinacyjnej (SCA) linii. Na uwagę zasługują mieszańce 1553-4 × K202XK208, 1560-17 × 324A2 i 1481-7 × S245, 1560-5 × K186XK194 (doświadczenie TD1) i mieszaniec 1587-1 × S41324A2 (doświadczenie TD2).
EN
Combining ability of crosses, which were obtained by crossing two groups of maize lines (each group consisted of 12 lines) with four testers was evaluated. The experiments were carried out using incomplete block design. Data were recorded on grain yield, dry matter content, smuttiness of plants and tillering. Analysis of GCA and SCA was made. Significant effects were detected for 1553-4 × K202xK208, 1560-17 × S41324A2 and 1481-7 × S245, 1560-5 × K186xK194 hybrids (TD1 experiment) and for 1587-1 × S41324A2 hybrid (TD2 experiment).
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