Wyniki wyszukiwania - Biblioteka Nauki

1

In situ hybridization-based detection of microRNAs in human diseases

100%

Zhang X. , Lu X. , Lopez-Berestein G. , Sood A. K. , Calin G.

microRNA Diagnostics and Therapeutics

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2014

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tom 1

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nr 1

EN

MicroRNAs (miRNAs) are small non-coding RNAs that regulate various aspects of gene expression in physiology and development. Links between miRNAs and the initiation and progression of human diseases are becoming increasingly apparent. The development of methods to detect the subcellular and tissue localization of miRNAs is essential for understanding their biological role in homeostasis. In this review, we discuss how in situ hybridization can complement tissuelevel miRNA expression profiling and its role as an investigational tool to better understand the etiology of human diseases. Furthermore, in situ hybridization of miRNAs represents a potent diagnostic assay that could be further refined and utilized for clinical applications.

2

Developmental regulation of Ubc9 in the rat nervous system

88%

Watanabe M. , Takahashi K. , Tomizawa K. , Mizusawa H. , Takahashi H.

Acta Biochimica Polonica

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2008

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tom 55

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nr 4

681-686

EN

The SUMO-conjugating enzyme Ubc9 is an essential enzyme in the SUMO (small ubiquitin-related modifier) protein modification system. Although sumoylation, covalent modification of cellular proteins by SUMO, is considered to regulate various cellular processes, and many substrates for sumoylation have been identified recently, the regulation of Ubc9 expression has not been examined in detail. We analyzed the expression of Ubc9 during rat brain development at the mRNA and protein levels. Northern and Western blot analyses revealed that expression of Ubc9 and SUMO-1 was developmentally regulated, while that of the ubiquitin-conjugating enzyme UbcH7 did not change so dramatically. In situ hybridization analysis revealed that the expression of Ubc9 was high in neuronal stem cells and moderate in differentiated neurons at embryonic stages. In the adult brain, moderate expression was observed in subsets of neurons, such as the dentate granular neurons and pyramidal neurons in the hippocampal formation and the large pyramidal neurons in the cerebral cortex. These results suggest that the Ubc9-SUMO system might participate in the proliferation and differentiation of neuronal cells in the developing brain and in neuronal plasticity in the adult brain.

3

Hybridocytochemistry - state of the art

75%

Van Der Ploeg M.

Postępy Biologii Komórki

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1989

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tom 16

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nr 4

4

Cross-species hybridizations in situ of genes associated with meat production traits in the wild pig genome

63%

Kozubska-Sobocinska A. , Danielak-Czech B. , Babicz M.

Annales Universitatis Mariae Curie-Skłodowska. Sectio EE: Zootechnica

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2015

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tom 33

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nr 3

5

Expression of the growth hormone gene in the chicken during embryonic and posthatch development

63%

Grossmann R. , Muhlbauer E. , Ellendorff F. , Jozsa R. , Reiprich K.

Zeszyty Naukowe. Przegląd Hodowlany

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1997

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tom 31

6

Study on breast carcinoma Her2-neu and hormonal receptors status assessed by automated images analysis systems: ACIS III [Dako] and ScanScope [Aperio]

63%

Slodkowska J. , Filas V. , Buszkiewicz E. , Trzeciak P. , Wojciechowski M. , Koktysz R. , Staniszewski W. , Breborowicz J. , Rojo M. G.

Folia Histochemica et Cytobiologica

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2010

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tom 48

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nr 1

19-25

7

Poly(A) RNA accumulation-storage in Cajal bodies in the plant cells

63%

Niedojadlo J. , Kubicka E. , Kalich B. , Bednarska-Kozakiewicz E.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

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tom 94

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nr 3

8

Molecular characterization and functional analysis of a glutathione peroxidase gene from Aphelenchoides besseyi (Nematoda: Aphelenchoididae)

63%

Wang B.-Y. , Wen R.-R. , Ma L.

Acta Parasitologica

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2017

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tom 62

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nr 3

9

Comparison of the sensitivity and specificity of real-time PCR and in situ hybridization in HPV16 and 18 detection in archival cervical cancer specimens

63%

Biesaga B. , Szostek S. , Klimek M. , Jakubowicz J. , Wysocka J.

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tom 50

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nr 2

10

Histone mRNA in situ hybridization in assessing proliferative activity of normal and malignant cells

63%

Slowinski J. , Mazurek U. , Bierzynska-Macyszyn G.

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2002

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tom 40

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nr 4

11

rRNA gene expression and location in triticale assayed by silver staining and in situ hybridisation techniques

63%

Lima-Brito J. , Carvalho A. , Matos C. , Heslop-Harrison P. , Guedes-Pinto H.

Plant Breeding and Seed Science

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2005

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tom 51

12

Structure and functions of lampbrush chromosomes

51%

Andraszek K. , Smalec E.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2011

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tom 92

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nr 4

13

Inflammatory bowel disease — is there something new in the immunological background?

51%

Marszalek A. , Marciniak R. , Szkaradkiewicz A. , Wasilewska A. , Chudzicka-Strugala I. , Ziuziakowska H. , Zebrowska M. , Majewski P. , Banasiewicz T. , Drews M.

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nr 2

14

FMRFamide-related peptides regulate the prothoracic gland activity

51%

Yamanaka N. , Zitnan D. , Hua Y.-J. , Mizoguchi A. , Tanaka Y. , Kataoka H.

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2005

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nr 3

147-152

15

Effect of human papillomavirus on cell cycle-related proteins p16INK4A, p21waf1/cip1, p53 and cyclin D1 in sinonasal inverted papilloma and laryngeal carcinoma. An in situ hybridization study

51%

Stasikowska-Kanicka O. , Wągrowska-Danilewicz M. , Danilewicz M.

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tom 49

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nr 1

16

Relative transcript abundance of oxytocin receptor gene in porcine uterus during luteolysis and early pregnancy

51%

Oponowicz A. , Franczak A. , Kurowicka B. , Kotwica G.

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nr 4

EN

The aim of the present study was to investigate transcript localization of the oxytocin receptor (OTR) gene in different cells of the porcine uterus during luteolysis and early pregnancy (days 14-16) using in situ hybridization (ISH). OTR mRNA was localized in the uterine luminal epithelium (LEC), glandular epithelium (GEC), stromal cells (SC) of the endometrium, in the longitudinal muscle layer (LM) and circular muscle layer (CM) of the myometrium. The OTR transcript was quantified by optical density units of silver grains. The OTR transcript levels in the endometrium and myometrium were statistically higher during luteolysis than during early pregnancy (P < 0.05). Besides, during luteolysis, the mRNA level was higher in the LEC, GEC of the endometrium and LM of the myometrium compared to that observed in the SC of endometrium and CM of the myometrium, respectively (P < 0.05). In summary: 1) the level of OTR mRNA in uterine tissues is higher during luteolysis compared to early pregnancy, 2) the OTR transcript level in endometrial cells did not correspond to the sensitivity of these cells to oxytocin (ОТ), 3) the myometrial expression of the OTR gene is appropriate to control contractile activity and secretion of PG during luteolysis.

17

En-Spm-like transposons in Poaceae species: transposase sequence variability and chromosomal distribution

51%

Altinkut A. , Raskina O. , Nevo E. , Belyayev A.

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tom 11

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nr 2

EN

Belonging to Class II of transposable elements, En/Spm transposons are widespread in a variety of distantly related plant species. Here, we report on the sequence conservation of the transposase region from sequence analyses of En/Spm-like transposons from Poaceae species, namely Zingeria biebersteiniana, Zingeria trichopoda, Triticum monococcum, Triticum urartu, Hordeum spontaneum, and Aegilops speltoides. The transposase region of En/Spm-like transposons was cloned, sequenced, and compared with equivalent regions of Oryza and Arabidopsis from the gene bank database. Southern blot analysis indicated that the En/Spm transposon was present in low (Hordeum spontaneum, Triticum monococcum, Triticum urartu) through medium (Zingeria bieberstiana, Zingeria trichopoda) to relatively high (Aegilops speltoides) copy numbers in Poaceae species. A cytogenetic analysis of the chromosomal distribution of En/Spm transposons revealed the concurence of the chromosomal localization of the En/Spm clusters with mobile clusters of rDNA. An analysis of En/Spm-like transposase amino acid sequences was carried out to investigate sequence divergence between 5 genera — Triticum, Aegilops, Zingeria, Oryza and Arabidopsis. A distance matrix was generated; apparently, En/Spm-like transposase sequences shared the highest sequence homology intra-generically and, as expected, these sequences were significantly diverged from those of O. sativa and A. thaliana. A sequence comparison of En/Spm-like transposase coding regions defined that the intra-genomic complex of En/Spm-like transposons could be viewed as relatively independent, vertically transmitted, and permanently active systems inside higher plant genomes. The sequence data from this article was deposited in the EMBL/GenBank Data Libraries under the accession nos. AY707995-AY707996-AY707997-AY707998-AY707999-AY708000-AY708001-AY708002-AY708003-AY708004-AY708005-AY708005-AY265312.

18

The identification and characterization of a testis-specific cDNA during spermatogenesis

51%

Chen Y. , Hu J. , Song P. , Gong W.

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tom 11

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nr 1

EN

Using bioinformatics and experimental validation, we obtained a cDNA (named srsf) which was exclusively expressed in the mouse testes. RT-PCR analysis showed that srsf mRNA was not expressed in the gonad during the sex determination period or during embryogenesis. In developing mouse tests, srsf expression was first detected on post-natal day 10, reached its highest level on day 23, and then reduced to and remained at a moderate level throughout adulthood. In situ hybridization analysis demonstrated that srsf mRNA was expressed in pachytene spermatocytes and round spermatids in the testes. The predicted protein contains one RNA-binding domain (RBD) and a serine-arginine rich domain (RS), which are characterized by some splicing factors of SR family members. These findings indicate that srsf may play a role during spermatogenesis.