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EN
There is growing evidence suggesting that the magnitude of cortisol awaking response (CAR), which is characterized by a profound increase of salivary cortisol secretion after awakening, plausibly reflects the level of a chronic stress, social stress, anxiety, etc. In this study the alternation of CAR at the start and at the end of two weeks session of self-instruction for good sleep was investigated; by which we anticipated that the self-instruction for good sleep would bring-forth a positive affection for the participants, and would result in decline of cortisol awakening response (CAR). Nevertheless, as a result unexpectedly, subjects did not change their sleep and dietary habits along with the instruction, moreover the increased CAR was observed. This result implies that the suggestion of an impractical instruction would solely be taken as a stressful task for participants, even though they know that it is effective to improve their sleep. On the contrary, if one develops an instruction with practicable indication for daily life, it is highly possible to observe a positive effect of the instruction on CAR.
EN
The Recent rapid growth of the information technology aid people to improve the performance in processing their job activities. These changes, in turn, force them to carry out their job activities using computers in the workplace; that results in the increase of somatic and/or mental stresses. In this study, we conducted a preliminary experiment to estimate an impact of web-browsing on human mind and body. Two types of web-browsing tasks, which are a 18-minuetes of continuous web-browsing and an intermittent web-browsing (first 45 second of each 1-minutes interval), were given to the subjects (10 healthy male aged from 20-23) with within-subjects experimental design. With regard to physiological measures, two prominent stress biomarkers, salivary immunoglobulin A (IgA) and cortisol, were employed. Comparing among the task conditions, relatively larger and long-lasting increase of IgA and smaller decrease of cortisol was observed with an intermittent web-browsing despite there was no difference in the psychological state. These results illustrate the difficulty of estimating the mental workload caused by web-browsing and the importance of employing physiological indices.
EN
Resistance to tuberculosis (TB) is cell-mediated but a humoral response is common and may be correlated with the lack of effective local cellular defense mechanisms. The goal of the study was to evaluate IgG, IgA, and IgM-mediated humoral immune response against 38-kDa+16-kDa and 38-kDa+lipoarabinomannan (LAM) mycobacterial antigens in bronchoalveolar fluid (BALF) from patients with pulmonary TB. Non-tuberculosis (NTB) patients were used as control. 179 BALF samples (56 TB and 123 NTB) were examined. Commercially available ELISA-based assays against proteins 38-kDa and 16-kDa or 38-kDa plus LAM were used. Three different dilutions of BALF: 1:1; 1:10, and 1:50 (100) were tested. Only the results obtained with the 1:10 dilution allowed distinguishing TB and NTB groups. The mean IgG level for 38-Da+LAM was significantly higher in the TB than that in the NTB group (P<0.0001). The mean IgA level for 38-kDa+LAM also was higher in the TB group (P<0.05). No difference was observed between TB and NTB groups in the titer of IgM antibodies. These findings indicate that TB is associated with the presence of detectable levels of antibodies in BALF. The antibody response is highly heterogeneous. This phenomenon results from the balance between pathogen and host immune system. The tests examined for detection of IgG in BALF can be used in combination with other diagnostic methods to increase diagnostic accuracy of pulmonary TB.
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EN
Different clinical outcomes of tuberculosis (TB) are related to the balance between cell-mediated and humoral immunity and may depend on environmental and individual factors including age and sex. The purpose of this study was to analyze the humoral immune response to recombinant and native mycobacterial antigens in relation to clinical presentations of pulmonary TB in children. We examined 224 serum samples including 81 primary and 31 postprimary TB cases, 30 cases of latent TB infection, and 82 nontuberculosis controls. Commercially available ELISA assays detecting IgG, IgA, and IgM against antigens: 38 kDa, 16 kDa, 38kDa, lipoarabinomannan (LAM), and A-60 were used. The results indicate that IgG production was very low in primary compared with postprimary TB (P<0.0001). IgM levels did not differ between the examined groups. Antibody levels strongly depended on the child's age. In infants aged below 1 there was no difference in the antibody level between the TB and control cases. Most positive cases were observed in children aged above 10. The influence of BCG vaccination on the antibody level was not seen. In all subgroups, person-to-person heterogeneity of antigen recognition was observed. We conclude that humoral immune response is associated with the phase of TB and is stronger in more advanced TB forms. IgG and IgA production against mycobacterial antigens is very low in young children.
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