Wyniki wyszukiwania - Biblioteka Nauki

1

Development and immunogenic properties of experimental vaccine against Lyme disease in dogs

100%

Mizak B. , Krol J.

Bulletin of the Veterinary Institute in Puławy

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2002

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tom 46

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nr 2

2

Construction and immunogenic properties of DNA vaccine against canine parvovirus infection

100%

Mizak B. , Rzezutka A. , Krol J. , Kazula A.

Bulletin of the Veterinary Institute in Puławy

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2001

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tom 45

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nr 2

EN

The presented studies were concentrated on construction of the recombinant mammalian expression vector - pSecTag2B, carrying canine parvovirus VP2 gene and its N-terminal fragment. Evaluation of safety of experimental vaccines was done on laboratory animals and immunogenic properties were tested on dogs. CPV specific antibodies were not detected by HI test in serum samples collected from dogs vaccinated with recombinant plasmid pSecTag2B-CPV582. The presence of CPV specific antibodies (80-320 by HI) was confirmed 10 days after vaccination in all dogs vaccinated with pSecTag2B-CPV1774. The highest titre was obtained in sera collected from dogs vaccinated with 200 μg and 300 μg of DNA. Fourteen days after revaccination, the level of antibodies increased up to 160 in dogs vaccinated with 100 μg of DNA and up to 640 in puppies vaccinated with 200 μg and 300 μg of DNA. The antibodies were detected for at least 16 weeks. Results of the studies demonstrated the immunogenic properties of elaborated DNA vaccine.

3

Immunogenic properties of Pasteurella multocida strains isolated from rabbits evaluated in white mice

84%

Borkowska-Opacka B. , Rutkowska-Jurga I. , Truszczynski M.

Bulletin of the Veterinary Institute in Puławy

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1995

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tom 39

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nr 2

4

Comparison of immunogenic properties of native and inactivated Mannheimia haemolytica Lkt in calves

67%

Wernicki A. , Mikucki P. , Urban-Chmiel R. , Puchalski A.

Polish Journal of Veterinary Sciences

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2008

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tom 11

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nr 2

83-88

EN

The aim of this study was to compare the immunostimulatory properties of Lkt of M. haemolytica inactivated by formaldehyde and glutaraldehyde and to evaluate the neutralizing properties of anti-Lkt antibodies. The experiment was conducted on 20 Black-and-White Lowland calves of 100 kg body weight, assigned to 4 experimental groups. The animals were given subcutaneous vaccine injections with native Lkt, Lkt inactivated by formaldehyde or Lkt inactivated by glutaraldehyde. The anti-Lkt antibody titres were measured using an enzyme-linked immunosorbent assay (ELISA), based on absorb- ance of the sera obtained from the animals immunized with the different forms of Lkt. The protective effects of the antibodies present in the sera isolated from the vaccinated animals were estimated using an MIT assay. Analysis of the ELISA absorbance values in the sera from calves in the vaccinated groups did not show any significant differences between the groups. The highest increase in absorbance of sera was observed in calves from the group that received formaldehyde-inactivated Lkt. In the case of calves immunized with native Lkt, the absorbance values were lower than in the group immunized with Lkt inactivated by formaldehyde. The lowest absorbance values were observed in sera obtained from calves vaccinated with Lkt inactivated by glutaraldehyde. Analysis of the MTT assay results revealed the greatest Lkt-neutralizing properties of antibodies in the sera of calves immunized with two doses of a vaccine containing native Lkt and Lkt inactivated with formaldehyde.

5

Serological and allergic response of calves experimantally infected with IPV or IBR strains of bovine herpesvirus type 1 [BHV1]

67%

Wisniewski J. , Rutherford M. , Swiecicka-Grabowska G. , Rotkiewicz Z. , Wojcik R.

Acta Academiae Agriculturae ac Technicae Olstenensis. Veterinaria

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1996

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tom 24

93-100

EN

Eight ten-week old calves, seronegative to BHVl,were divided at random into 2 groups of four. One group was infected with the Cooper (IBR) strain, while the other group was treated with the 509/89 (IPV) strain. Their humoral immunologic response to the virus was cross tested using the seroneutralisation (SN) test (with both strains) and an immunoenzymatic (IBR - EIA, Svanova) test. However, allergic skin tests using two preparations that were obtained from the above mentioned strains,were applied in the same way as in a tuberculin test. The tests showed that immunogenic properties of the used strains are similar (the mean titres of antibodies in both groups did not differ significantly) but they differ as far as antigenic features are concerned. Lower titres of antibodies were detected with the 508/89 (IPV) strain than with the Cooper (IBR) strain. Humoral immunologic response, tested with EIA, was positive in calves infected with the (IPV) strain (on the 35th day p.i.) and negative in the Cooper (IBR) strain infected group. Allergic tests using „homo- and heterological" preparations, showed that a delayed type of hypersensitivity (DTH) of skin developed in animals from the IPV group on day 35 p.i. (heterological preparation), whereas on day 56 p.i. in the case of the homological preparation. The DTH of skin was maintained up to the end of the experiment. Calves from the IBR group, tested for skin allergy with both preparations, reacted only sporadically positively. The possible reasons for the confirmed differences have been discussed.

PL

Osiem 10-tygodniowych cieląt, seroujemnych wobec BHV1, podzielono losowo na dwie grupy po 4 osobniki i zakażono jedne szczepem Cooper (IBR), drugie szczepem 508/89 (IPV). Ich odpowiedź humoralną na wirus badano krzyżowo odczynem seroneutralizacji przy użyciu obu szczepów i immunoenzymatycznie, a odczyn alergiczny skóry dwoma preparatami uzyskanymi we własnym zakresie z wyżej wymienionych szczepów, które aplikowano jak w badaniu tuberkulinowym. Wykazano, iż właściwości immunogenne badanych szczepów są podobne (zbliżone poziomy średnich mian przeciwciał w obu grupach), natomiast różnią się w zakresie antygenowym szczepem IPV (508/89), użytym jako antygen diagnostyczny, wykrywano bowiem niższe miana niż stosując w tym celu szczep Cooper. Odpowiedź humoralna badana odczynem EIA byla pozytywna u zwierząt zakażonych szczepem IPV; nie wykazano natomiast serokonwersji u cieląt pozostałej grupy. Badaniem alergicznym z zastosowaniem preparatów „homo- i heterologicznego" wykazano, że nadwrażliwość skóry typu późnego na preparat heterologiczny rozwinęła się u osobników grupy IPV już w 35., na preparat homologiczny zaś dopiero w 56. dniu po zakażeniu. Wykazana NTP utrzymywała się u reagentów do końca eksperymentu. Cielęta grupy IBR badane obydwoma preparatami reagowały sporadycznie dodatnio. Omówiono możliwe przyczyny stwierdzonych różnic.

6

Comparison of two vaccines against bovine leukemia virus [BLV], containing formalin treated or native virus proteins

67%

Grundboeck M. , Buzala E. , Szczotka M.

Bulletin of the Veterinary Institute in Puławy

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1992

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tom 36

7

Immunogenic properties of the Polish Campylobacter strains isolated from sheep

67%

Bronicka A.

Bulletin of the Veterinary Institute in Puławy

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1998

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tom 42

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nr 1

EN

Forty-three C.f.s.f. strains and 19 C. jejuni strains from miscarried sheep foetuses and rectal mucosa smears were isolated. The C.f.s.f. strains were used to prepare 4 vaccines. The dynamics of the occurrence of antibodies against anti-Campylobacter in the sheep immunized with these vaccines was similar. The highest mean titres were found from experimental weeks 8 to 10. The vaccine applied in disease focus revealed satisfactory protective properties. The results obtained made it possible to select the strains which can be used for immunization of sheep.