Wyniki wyszukiwania - Biblioteka Nauki

1

The presence of anti-Borrelia burgdorferi antibodies in person with suspected Lyme disease

100%

Tokarska-Rodak M. , Plewik D. , Galecka B. , Domanski R.

Health Problems of Civilization

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2016

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tom 10

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nr 3

EN

Background: Lyme disease is a multi-organ disease caused by spirochetes, Borrelia burgdorferi sensu lato, transmitted by Ixodes, with its clinical picture including involvement of the skin, joints, nervous system and heart. Laboratory diagnostic tests for Lyme disease are mainly based on the detection of anti-Borrelia burgdorferi antibodies by means of serological methods. Aim of the work: assessment of the level of antibodies against specific B. burgdorferi s.l. antigens in persons with suspected Lyme disease. Material and methods: the tested group consisted of 98 patients with suspected Lyme disease. During the first phase of the tests, anti-Borrelia burgdorferi IgM/IgG antibodies were marked using ELISA method, and positive and uncertain results were confirmed by Westernblot test (Wb). Results: anti-B. burgdorferi IgM/IgG antibodies were present in 60 patients (61.2%). IgM and IgG antibodies were detected as positive in 8 (8.1%) and 35 (35.7%) patients respectively. IgM and IgG were co-present in 6 persons (6.1%), including 2 persons (2%) with positive results in both classes. All patients with positive IgM (12 persons) had anti-OspC antibodies, and 2 patients had, in addition, anti-p31 antibodies. In patients with positive IgG the results were as follows: antibodies against antigen p17 - 77% of cases, VlsE - 74%, p30 - 46%, p39 - 44%, p83 - 38%, p19 - 31%, OspC/p25- 28%, p31 - 23%, p21 - 8%. Conclusions: laboratory diagnostic tests for Lyme disease must be performed in accordance with the current standards, positive and uncertain results must be confirmed by Westernblot test. Results of lab tests must correlate with patient’s symptoms.

PL

Wprowadzenie: Borelioza z L yme jest wielonarządową chorobą wywoływaną przez krętki Borrelia burgdorferi sensu lato, przenoszone przez kleszcze Ixodes, której obraz kliniczny wiąże się z zajęciem skóry, stawów, układu nerwowego i serca. Diagnostyka laboratoryjna boreliozy z L yme opiera się głównie na wykrywaniu przeciwciał anty-Borrelia burgdorferi metodami serologicznymi. Cel pracy: ocena poziomu przeciwciał dla specyficznych antygenów B. burgdorferi s.l. u osób z podejrzeniem boreliozy z L yme. Materiały i metody: grupę badaną stanowiło 98 pacjentów z podejrzeniem boreliozy z L yme. W pierwszym etapie wykonano oznaczenie przeciwciał IgM/IgG anty-Borrelia burgdorferi metodą ELISA, a wyniki pozytywne i graniczne potwierdzono testem Western blot (Wb). Wyniki: obecność przeciwciał IgM/IgG anty-B. burgdorferi wykazano u 60 pacjentów (61,2%). Przeciwciała tylko w klasie IgM oraz tylko IgG na poziomie dodatnim stwierdzono odpowiednio u 8 (8,1%) oraz 35 (35,7%) pacjentów. Współistnienie IgM i IgG stwierdzono u 6 osób (6,1%), w tym u 2 (2%) na poziomie dodatnim w obu klasach. U wszystkich pacjentów z pozytywnym wynikiem w klasie IgM (12 osób) obecne były przeciwciała anty-OspC, u 2 pacjentów dodatkowo obecne były przeciwciała anty-p31. U pacjentów z pozytywnym wynikiem w klasie IgG uzyskano następujące wyniki: przeciwciała przeciwko antygenowi p17 - 77% przypadków, VlsE - 74%, p30 - 46%, p39 - 44%, p83 - 38%, p19 - 31%, OspC/p25- 28%, p31 - 23%, p21 - 8%. Wnioski: prowadząc diagnostykę laboratoryjną boreliozy z L yme należy postępować zgodnie z obowiązującymi standardami, wyniki dodatnie i graniczne uzyskane metodą ELISA, należy potwierdzić testem Western blot. Wyniki badań laboratoryjnych muszą korelować z objawami występującymi u pacjenta.

2

Evaluation of the protective effect of a prime-boost strategy with plasmid DNA followed by recombinant adenovirus expressing BmAMA1 as vaccines against Babesia microti infection in hamster

84%

Wang G. , Yu L. , Efstratiou A. , Moumouni P. F. A. , Liu M. , Guo H. , Gao Y. , Cao S. , Zhou M. , Li J. , Ringo A. E. , Xuan X.

Acta Parasitologica

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2018

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tom 63

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nr 2

3

Diagnostic value of semi-purified antigens of hydatid cyst fluid in human cystic echinococcosis

84%

Iraqi W.

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tom 61

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nr 1

4

Changes of peroxiredoxins in beech seeds (Fagus sylvatica L.) during development and maturation

84%

Ratajczak E. , Kalemba E. M. , Pukacka S.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

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tom 94

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nr 3

5

Iron shortage and bile salts play a major role in the expression of ompK gene in Vibrio anguillarum

84%

Hamod M. A. , Bhowmick P. P. , Shukur Y. N. , Karunasagar I. , Karunasagar I.

Polish Journal of Microbiology

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2014

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tom 63

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nr 1

EN

The outer membrane protein K, OmpK first identified in Vibrio parahaemolyticus has been shown to be a receptor for a broad host range vibriophage KVP40 infecting members of the Vibrionaceae. In the study, the effect of culture conditions on the expression of ompK in V. anguillarum was studied using real-time PCR. The expression increased significantly in the presence of bile salts and iron chelating agent 2, 2’ bipyridine, suggesting a role for this protein in bile resistance and also in iron acquisition by V. anguillarum. OmpK induction by iron limitation and the presence of bile salts was reconfirmed by western blot technique after growing the cells in trypticase soy broth supplemented with bile salts, blood and 2, 2’ bipyridine. We surmise that the expression of OmpK protein of V. anguillarum is bile salt and iron chelating agent-dependent.

6

Increased skeletal muscle expression of VEGF induced by massage and exercise

84%

Andrzejewski W. , Kassolik K. , Kobierzycki C. , Grzegrzolka J. , Ratajczak-Wielgomas K. , Jablonska K. , Halski T. , Dziegiel P. , Gworys B. , Podhorska-Okolow M.

Folia Histochemica et Cytobiologica

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2015

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tom 53

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nr 2

7

Expression of hepatitis B core antigen in lettuce and tobacco for advanced plant-derived vaccine formulations

84%

Pyrski M. , Kasprzyk A. , Bogusiewicz M. , Bociag P. , Czyz M. , Pniewski T.

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2015

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tom 96

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nr 1

8

Talin, vinculin and nestin expression in orofacial muscles of dystrophin deficient mdx mice

84%

Spassov A. , Gredes T. , Pavlovic D. , Gedrange T. , Lehmann C. , Lucke S. , Kunert-Keil C.

Archivum Immunologiae et Therapiae Experimentalis

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2012

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tom 60

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nr 2

9

Production and preliminary evaluation of Trypanosoma evansi HSP70 for antibody detection in Equids

84%

Kumar J. , Chaudhury A. , Bera B. C. , Kumar R. , Kumar R. , Tatu U. , Yadav S. C.

Acta Parasitologica

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2015

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tom 60

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nr 4

10

Expression of FOXJ1 and ITGB4 is activated upon KLH and LTA stimulation in the DT40 cell line

84%

Dunislawska A. , Slawinska A. , Siwek M.

Folia Biologica

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2017

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tom 65

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nr 1

11

Expression of peroxiredoxins in the human testis, epididymis and spermatozoa and their role in preventing H2O2-induced damage to spermatozoa

84%

Shi H. , Liu J. , Zhu P. , Wang H. , Zhao Z. , Sun G. , Li J.

Folia Histochemica et Cytobiologica

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2018

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tom 56

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nr 3

12

alpha2beta1 integrin-mediated mechanical signals during osteodifferentiation of stem cells from the Wharton’s jelly of the umbilical cord

84%

Witkowska-Zimny M. , Wrobel E. , Mrowka P.

Folia Histochemica et Cytobiologica

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2014

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tom 52

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nr 4

13

Orexin A modulates endocrine function and viability of porcine pancreatic islets

84%

Sassek M. , Pruszynska-Oszmalek E. , Nowak K. W.

Journal of Physiology and Pharmacology

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2017

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tom 68

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nr 6

14

WNT7A expression is downregulated in T lymphocytes after T-cell receptor activation due to histone modifications and in T-ALL by DNA methylation

84%

Barreto-Vargas C. , Alvarez-Zavala M. , Garcia-Chagollan M. , Hernandez-Flores G. , Aguilar-Lemarroy A. , Jave-Suarez L. F.

Archivum Immunologiae et Therapiae Experimentalis

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2020

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tom 68

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nr 3

15

Complement inhibitory proteins expression in placentas of thrombophilic women

84%

Wirstlein P. K. , Jasinski P. , Rajewski M. , Gozdziewicz T. , Skrzypczak J.

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tom 50

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nr 3

16

Induction of autophagy in the porcine corpus luteum of pregnancy following anti-androgen treatment

84%

Grzesiak M. , Knapczyk-Stwora K. , Slomczynska M.

Journal of Physiology and Pharmacology

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2016

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tom 67

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nr 6

17

mRNA and protein expression of FGF-1, FGF-2 and their receptors in the porcine umbilical cord during pregnancy

67%

Chrusciel M. , Rekawiecki R. , Ziecik A. , Andronowska A.

Folia Histochemica et Cytobiologica

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2010

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tom 48

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nr 4

18

Characterisation of NcGRA7 and NcSAG4 proteins: immunolocalisation and their role in the host cell invasion by Neospora caninum tachyzoites

67%

Aguado-Martinez A. , Alvarez-Garcia G. , Schares G. , Risco-Castillo V. , Fernandez-Garcia A. , Marugan-Hernandez V. , Ortega-Mora L. M.

Acta Parasitologica

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2010

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tom 55

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nr 4

304-312

EN

Neospora caninum negatively impacts bovine reproductive performance around the world. Addressing this problem requires a greater understanding of the parasite’s molecular biology. In this study, monoclonal antibodies against recombinant proteins were successfully developed and employed to characterise two different proteins of N. caninum: the acute phase-associated NcGRA7 and the chronic phase-associated NcSAG4. Immunofluorescence with the anti-rNcGRA7 monoclonal antibody suggested that NcGRA7 trafficks from tachyzoite dense granules to the matrix of the parasitophorous vacuole and parasite’s surroundings. Furthermore, NcGRA7 is also expressed in the bradyzoite stage and localised on the matrix of bradyzoite-positive vacuoles. NcGRA7 appears to be partially involved in the tachyzoite-invasion mechanisms, as an anti-rNcGRA7 monoclonal antibody partially inhibited in vitro tachyzoite-invasion. A monoclonal antibody specific for NcSAG4 confirmed this protein’s bradyzoitespecific expression both by western blot and immunofluorescence. However, some bradyzoite-positive vacuoles only weakly expressed NcSAG4, if it was expressed at all. The specificity of the anti-rNcSAG4 monoclonal antibody was confirmed by the recognition of the NcSAG4 in the membrane surface of Nc-1SAG4c transgenic tachyzoites, which constitutively expresses NcSAG4. Blocking NcSAG4 of Nc-1SAG4c tachyzoites with the monoclonal antibody did not affect host cell invasion. However, its implication on the host cell adhesion or host immune evasion should not be discarded.

19

Expression of steroidogenic enzymes in porcine polycystic ovaries

67%

Kozlowska A. , Majewski M. , Jana B.

Folia Histochemica et Cytobiologica

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2009

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tom 47

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nr 2

257-264

20

Beclin 1 is involved in regulation of apoptosis and autophagy during replication of ectromelia virus in permissive L929 cells

67%

Martyniszyn L. , Szulc L. , Boratynska A. , Niemialtowski M. G.

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2011

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tom 59

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nr 6