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Immobilized cells of recombinant Escherichia coli strain for continuous production of L-aspartic acid

100%

Szymanska G. , Sobierajski B. , Chmiel A.

nr 2

For L-aspartic acid biosynthesis, high production cells of Escherichia coli mutant B-715 and P1 were immobilized in chitosan gel using a technique developed in our laboratory. The immobilization process reduced initial activity of the intact cells, however, the biocatalyst produced was very stabile for long-term use in multi-repeated batch or continuous processes. Temperature influence on the conversion of ammonium fumarate to L-aspartic acid was investigated. In long-term experiments, over 603 hours, the temperature 40°C was found to be the best for both biocatalyst stability and high conversion rate. The optimum substrate concentration was 1.0 M. Continuous production of L-aspartic acid was investigated in three types of column bioreactors characterized by different volumes as well as different high to biocatalyst bed volume rations (Hz/Vz). The highest conversion rate, 99.8%, and the productivity 6 g/g/h (mass of L-aspartic acid per dry mass of cells in biocatalyst per time unit) was achieved in the bioreactor with the highest value Hz/Vz = 3.1, and liquid hour space velocity value of 5.2, defined as the volume of feeding substrate passed per volume of catalyst in bioreactor per one hour.

Application of porous ceramic sinter as a support for immobilization of Saccharomyces cerevisiae yeast cells

100%

Janiszyn Z. , Dziuba E. , Boruczkowski T. , Chmielewska J. , Kawa-Rygielska J. , Rosiek G.

nr 4[B]

Ethanol fermentation with yeast cells immobilized on grains of porous ceramic sinter

100%

Janiszyn Z. , Dziuba E. , Boruczkowski T. , Chmielewska J. , Kawa-Rygielska J. , Rosiek G.

nr 4[B]