Wyniki wyszukiwania - Biblioteka Nauki

1

Spatial variability in the activity of hydrolytic enzymes in a marine beach [Southern Baltic Sea]

100%

Mudryk Z. J. , Podgorska B.

Polish Journal of Ecology

|

2005

|

tom 53

|

nr 2

EN

Potential activity of hydrolytic microbial extracellular enzymes were determined in a sandy beach in Sopot, (Southern Baltic Sea) in July 2001. Among of the five monitored enzymes, the highest level of activity (314.28 nM MCA g⁻¹d.w. h⁻¹) was determined in the aminopeptidase whereas chitinase had the lowest level (0.50 nM MUF g⁻¹d.w. h⁻¹) of potential activity. Usually, the ranking of the activity rates of the assayed enzymes was following: aminopeptidase > lipase > β-glucosidase > α-glucosidase > chitinase. Clear gradients in the level of enzymatic activity were determined in horizontal profile of the beach. The maximum activity of studied enzymes was recorded in sand collected under seawater and at the waterline.

2

Hydrolytic enzymes of developing Ascaris lumbricoides suis eggs

100%

Wojcik A. , Blaszkowska J.

Wiadomości Parazytologiczne

|

1998

|

tom 44

|

nr 3

3

The effect of pulsation magnetic field on enzymatic activity of hydrolases of Candida albicans strains isolated from patients of risk group

100%

Glowacka A. , Budak A.

Wiadomości Parazytologiczne

|

1998

|

tom 44

|

nr 3

4

Protein inhibitors of serine proteinases

86%

Otlewski J. , Krowarsch D. , Apostoluk W.

Acta Biochimica Polonica

|

1999

|

tom 46

|

nr 3

EN

Serine proteinases and their natural protein inhibitors belong to the most intensively studied models of protein-protein recognition. Protein inhibitors do not form a single group but can be divided into about 20 different families. Global structures of proteins representing different inhibitor families are completely different and comprise α-helical proteins, β-sheet proteins,α/β-proteins and different folds of small disulfide-rich proteins. Three different types of inhibitors can be distinguished: canonical (standard mechanism) inhibitors, non-canonical inhibitors, and serpins. The canonical inhibitor binds to the enzyme through the exposed and convex binding loop, which is complementary to the active site of the enzyme. The mechanism of inhibition in this group is consistently very similar and resembles that of an ideal substrate. Non-canonical inhibitors, originating from blood sucking organisms, specifically block enzymes of the blood clotting cascade. The interaction is mediated through inhibitor N-terminus which binds to the proteinase forming a parallel β-sheet. There are also extensive secondary interactions which provide an additional buried area and contribute significantly to the strength and specificity of recognition. Serpins are major proteinase inhibitors occurring in plasma. Similarly to canonical inhibitors, serpins interact with their target proteinases in a substrate-like manner. However, in the case of serpins, cleavage of a single peptide bond in a flexible and exposed binding loop leads to dramatic structural changes.

5

Secretion of hydrolytic enzymes by fungal strains, isolated from patients with malignant tumors of head and neck, before, during and after radiotherapy

72%

Moqbil S. , Kurnatowski P.

Annals of Parasitology

|

2012

|

tom 58

|

nr 1

EN

One method of treatment used in cancer therapy is radiotherapy which can injure the oral, pharynx or larynx mucosa and predisposes tissue to the development of fungal infections. The aim of the study paper was the mycological examinations of swabs from the oral cavity and pharynx of patients obtained prior to, in week 3, on the last day of and 3 weeks after radiotherapy, as well as isolation of fungi and identification of the selected parameter of strains pathogenecity, i.e. hydrolytic enzyme release. Forty-three patients with oral cavity, pharynx or larynx carcinoma were examined at four points during a course of radiotherapy: before treatment, in week 3 of treatment, on the last day of treatment and 3 weeks afterwards. The mycological examination was conducted based on a procedure introduced in the Department of Biology and Medical Parasitology, Medical University of Lodz. The activity of the hydrolytic enzymes was evaluated with a bioMerieux API ZYM test kit. More than 2/3 of the patients (68.2%) were found to have a fungal infection in the first examination, 4/5 (80%) in the second, about 3/5 (57.1%) in the third and all (100%) in the last examination. The release of enzymes varied, and on different stages show different inactive enzymes: at the start, α-chymotrypsin and α-mannosidase; at 3 weeks, β-glucuronidase and α-mannosidase; at the end, α-chymotrypsin; at 3 weeks after the end, trypsin, α-chymotrypsin, α-galaktosidase and α-fucosidase. The most frequent species isolated from the patients treated by radiotherapy is Candida albicans and C. glabrata. The latter is characterized by resistance to the majority of antimycotic medications. The isolated strains are characterized by the highest activity of leucine arylamidase, acid phosphatase and naphthol – AS-BI-phosphohydrolase. Considering the enzymes produced, most of the strains can be included to biotypes D₃, C₆ and A.

6

Change in hydrolytic enzyme efficiency over time

72%

Szadkowska D.

Annals of Warsaw University of Life Sciences - SGGW. Forestry and Wood Technology

|

2020

|

tom 112

7

The fungi present in the sand and soil of recreational areas as possible factors of fungal infection among children: hydrolytic enzymes - a determinant of pathogenicity

72%

Kurnatowski P. , Wojcik A. , Blaszkowska J. , Goralska K.

Annals of Parasitology

|

2016

|

tom 62

|

nr Suppl.

8

Concentrations of available phosphorus and sulphur and activities of some hydrolytic enzymes in a luvisol fertilized with farmyard manure and nitrogen

72%

Lemanowicz J. , Siwik-Ziomek A.

Polish Journal of Soil Science

|

2010

|

tom 43

|

nr 1

EN

Concentrations of available phosphorus (PE-R), and sulphate sulphur (S-SO₄²⁻) as well as activities of alkaline (AlP) and acid phosphatase (AcP), and arylsulphatase were assayed in a Luvisol fertilized with various doses (0, 20, 40, 60, 80 t·ha⁻¹) of farmyard manure (factor I) and nitrogen in mineral form (0, 45, 90, 135 kg ha⁻¹, factor II). PE-Rand S-SO₄²⁻ concentrations were significantly affected by fertilization with FYM and nitrogen, with the highest increase noted for soil samples taken from the objects with the manure dose 60 t ha⁻¹. Nitrogen used in the dose 135 kg ha⁻¹ increased significantly S-SO₄²⁻ and decrease PE-Rcontent as compared with their concentrations recorded for lower doses of this fertilizer. The highest concentration of available phosphorus was found in the sample collected in September. However, activities of phosphatases observed in the same sample were the lowest. The lowest arylsulphatase activity was noted in July, while sulphate sulphur concentrations in the same sample was the highest.

PL

Badano zmiany zawartości fosforu przyswajalnego (PE-R), siarki siarczanowej (SSO₄), oraz aktywności fosfatazy alkalicznej (AlP) i kwaśnej (AcP), arylosulfatazy w glebie płowej nawożonej zróżnicowanymi dawkami obornika – I czynnik: (0, 20, 40, 60, 80 t ha⁻¹) i azotu w postaci saletry amonowej - II czynnik: (0, 45, 90, 135 kg ha⁻¹). Największą istotną zawartość przyswajalnych dla roślin fosforu i siarki stwierdzono w glebie pobranej z obiektów nawożonych obornikiem w dawce 60 t ha⁻¹. Azot w dawce N3 spowodował istotny zwiększenie zawartości SSO₄w badanej glebie płowej, natomiast obniżenie zawartości PE-R w porównaniu do niższych dawek tego nawozu mineralnego. Najwyższą zawartość fosforu przyswajalnego stwierdzono w glebie pobranej we wrześniu. W glebie pobranej w tym samym terminie aktywność fosfatazy alkalicznej i kwaśnej była najniższa. Aktywność arylosulfatazy była najniższa w glebie pobranej w lipcu. Natomiast zawartość siarki siarczanowej w glebie pobranej w lipcu była najwyższa.

9

Extracellular enzyme activity in the surface microlayer and subsurface water in the coastal lake Dolgie Wielkie

72%

Zdanowicz M. , Mudryk Z. J. , Perlinski P.

Baltic Coastal Zone. Journal of Ecology and Protection of the Coastline

|

2018

|

tom 22

EN

The paper presents studies on level hydrolytic activity of extracellular enzymes in the surface microlayer and subsurface water in the coastal lake Dołgie Wielkie. The ranking order of the potential enzyme activity rates in the studied water layers was as follows: aminopeptidase > lipase > α-glucosidase > β-glucosidase. The level of activity of all studied hydrolases was higher in the surface microlayer than subsurface water. Activity of extracellular enzymes was influenced by the season.

PL

W błonie powierzchniowej i w wodzie podpowierzchniowej przybrzeżnego jeziora Dołgie Wielkie badano aktywność enzymatyczną czterech enzymów hydrolitycznych z wykorzystaniem metody spektrofluorometrycznej. Uzyskane wyniki badań wykazały, że najwyższym poziomem aktywności enzymatycznej charakteryzowały się aminopeptydaza i lipaza. Poziom aktywności wszystkich badanych enzymów hydrolitycznych był wyższy w błonie powierzchniowej niż w wodzie podpowierzchniowej. W jeziorze Dołgie Wielkie stwierdzono dynamikę zmian sezonowych poziomu aktywności badanych ektoenzymów.

10

Factors affecting hydrolytic enzymes production by Yarrowia lipolytica strains

72%

Gdula A. , Chrzanowska J. , Szoltysik M. , Kiezel X. , Wojtatowicz M.

Acta Scientiarum Polonorum. Biotechnologia

|

2002

|

tom 01

|

nr 1-2

11

Enzyme profile in samples of bacterial cellulose film treated with Trametes versicolor and Coniophora puteana

72%

Betlej I.

Annals of Warsaw University of Life Sciences - SGGW. Forestry and Wood Technology

|

2021

|

tom 116

12

Factors affecting hydrolytic enzymes production by Yarrowia lipolytica strains

72%

Gdula A. , Chrzanowska J. , Szoltysik M. , Kiezel X. , Wojtatowicz M.

Acta Scientiarum Polonorum. Biotechnologia

|

2002

|

tom 01

|

nr 1-2

13

Effect of low molecular chitin on the production of proteins with antifungal activity by Trichoderma atroviride NBT-11

72%

Monte E. , Gomez M. I. , Guerra I. , Llobell A. , Bautista J.

Bulletin of the Polish Academy of Sciences. Biological Sciences

|

2003

|

tom 51

|

nr 1

14

Compositional and enzymatic changes in guava [Psidium guajava L.] fruits during ripening

72%

Jain N. , Dhawan K. , Malhotra S. P. , Siddiqui S. , Singh R.

Acta Physiologiae Plantarum

|

2001

|

tom 23

|

nr 3

15

Badanie zdolnosci szczepow termofilnego grzyba Thermomyces lanuginosus do hydrolizy tluszczu kakaowego oraz smalcu

58%

Janda K.

|

2004

|

tom 55

|

nr 1

83-87

PL

Thermomyces lanuginosus jest jednym z najpowszechniej występujących w przyrodzie grzybów termofilnych. Badania dowodzą, że szczepy tego gatunku zdolne są do biosyntezy wielu enzymów hydrolitycznych. Prace określające aktywność lipolityczną szczepów tego gatunku prowadzone były najczęściej na podłożu z dodatkiem oliwy z oliwek. Celem niniejszego opracowania była ocena zdolności szczepów Thermomyces lanuginosus do biosyntezy egzoenzymów lipolitycznych w obecności tłuszczów o konsystencji stałej.

EN

The purpose of the study was the estimation of the lipolytic activity of the thermophilic fungus Thermomyces lanuginosus on the solid base with the cocoa fat and the lard. The material was 144 strains isolated from biohumus, garden compost, leaf compost, mushroom compost, hazelnuts and raw coffee beans. The study proved, that all species was able to hydrolyze both the cocoa oil and the lard. The index of the lipolytic acivity was the same on the medium with cocoa oil and on the medium with the lard.

16

The effects of hydro- and osmopriming on the germination, vigour and hydrolytic enzymes activity of common zinnia (Zinnia elegans Jacq.) seeds

58%

Szopinska D. , Politycka B.

Folia Horticulturae

|

2016

|

tom 28

|

nr 1

17

Zbozowe bialkowe inhibitory enzymow hydrolitycznych i ich znaczenie. Czesc I. Bialkowe inhibitory alfa-amylaz

58%

Piasecka-Kwiatkowska D. , Warchalewski J. R.

Żywność Nauka Technologia Jakość

|

2000

|

tom 07

|

nr 2

110-119

PL

W ziarnie zbóż występują w znacznych ilościach białkowe substancje o charakterze inhibitorów enzymów hydrolitycznych. Pomimo, iż występowanie tych substancji w ziarnie zbóż stwierdzono już w latach trzydziestych, to do tej pory nie udało się dokładnie ich poznać. W pracy tej scharakteryzowano inhibitory enzymów amylolitycznych występujące w ziarnie pszenicy, żyta i pszenżyta oraz przedstawiono współczesne poglądy na temat ich znaczenia.

EN

Cereal seeds contain a lot of protein substances which are inhibitors of hydrolytic enzymes. Although, they were discovered in the early 1930's till today remain a number of question to be answer. In this paper protein nature alpha-amylase inhibitors found in grain of wheat, rye and triticale were discussed on the grounds of the present-day knowledge.

18

Distribution of tannic acid degrading microorganisms in the soil and comparative study of tannase from two fungal strains

58%

Mondal K. C. , Samanta S. , Giri S. , Pati B. R.

|

2001

|

tom 50

|

nr 1

19

Physiological and biochemical changes induced by osmolarity in halotolerant Aspergilli

58%

Mahdy H. M. , El-Sheikh H. H. , Ahmed M. S. , Refaat B. M.

Acta Microbiologica Polonica

|

1996

|

tom 45

|

nr 1

20

Zbozowe, bialkowe inhibitory enzymow hydrolitycznych i ich znaczenie. Czesc II: Bialkowe inhibitory proteinaz

51%

Piasecka-Kwiatkowska D. , Warchalewski J. R.

Żywność Nauka Technologia Jakość

|

2000

|

tom 07

|

nr 3

33-38

PL

W pracy przedstawiono przegląd danych literaturowych na temat zbożowych, białkowych inhibitorów enzymów proteolitycznych. Szczególną uwagę zwrócono na ich właściwości, formy wielorakie, a także ich znaczenie biochemiczne, fizjologiczne i żywieniowe.

EN

The present state of knowledge on cereal proteinacious inhibitors of proteolytic enzymes is reviewed. Particular attention was given on their properties, multiple forms as well as significance from nutritional, physiological and biochemical point of view.