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Hybrydyzacja in situ [ISH] w cytogenetyce

100%

Zawada M. , Latos-Bielanska A.

tom 20

nr 4

363-371

Wystepowanie poodsadzeniowego wielonarzadowego zespolu wyniszczajacego w krajowej populacji swin

84%

Podgorska K. , Stadejek T. , Szczotka A. , Kozaczynski W. , Porowski M. , Pejsak Z.

Medycyna Weterynaryjna

2009

tom 65

nr 05

330-333

The aim of the study was to assess the occurrence of the postweaning wasting syndrome (PMWS) in Polish pig herds. Thirty-two herds were selected from 195 visited pig production sites based on the appearance of clinical lesions. The population of breeding herds ranged from 25 to 2000 sows and the percentage of losses varied from 2 to 20%. In each farm 3 pigs affected by wasting were autopsied and lymph nodes were collected. Sections of lymph nodes were examined for the presence of histopathological lesions and tested with in situ hybridization to detect the DNA of porcine circovirus type 2 (PCV2), an etiological agent of PMWS. The results were considered as positive when characteristic histopathological changes were detected along with an abundant amount of viral DNA. PMWS was recognized in 26 pigs (27.9%) from 16 farms (50%). In two of those farms PMWS was diagnosed as sporadic, based on the analysis of production parameters and low rate of losses. In 12 herds (37.5%) no pig had DNA of PCV2 in lymph nodes, while in the remaining 4 herds (12.5%) low amounts of DNA were detected in lymph nodes from at least one pig. PMWS was confirmed in all tested farms with breeding herds of more than 1000 sows, while none of the small farms with breeding herds of less than 100 sows were affected. In two other groups of farms counting 100-499 and 500-1000 sows the percentage of affected herds was respectively 33.3% and 57.1%. The established rate of PMWS occurrence was similar to that claimed by most other European countries. Results obtained in this study suggest that the incidence of PMWS outbreaks increases with the size of the herd. A higher number of properly selected samples markedly enhances the probability of disease detection.

Zastosowanie metod biologii molekularnej w diagnostyce weterynaryjnej

84%

Winiarczyk S.

tom 49

nr 06

260-264

Wykazanie ekspresji mRNA genu c-myc w chloniakach u psow

84%

Winiarczyk S. , Zielewicz J. , Lopuszynski W. , Gradzki Z. , Nozdryn-Plotnicki Z. , Lechowski R. , Jagielski D.

nr 10

736-740

Porownanie ekspresji protoonkogenu c-myc w prawidlowym i nowotworowym endometrium u kobiet

84%

Skrzypczak M. , Zielewicz J. , Winiarczyk S. , Wojcierowski J. , Jakowicki J. A. , Kaczmarek L.

nr 11

1181-1185

Hybrydyzacja kwasow nukleinowych - zastosowanie w inzynierii genetycznej i diagnostyce

84%

Reichert M. , Grundboeck J.

tom 49

nr 06

256-260

Pierwszy przypadek poodsadzeniowego wielonarzadowego zespolu wyniszczajacego swin w Polsce

84%

Stadejek T. , Podgorska K. , Kolodziejczyk P. , Bogusz R. , Kozaczynski W. , Pejsak Z.

tom 62

nr 03

297-301

The present study describes first clinically detected and confirmed by laboratory methods case of PMWS in Poland. The investigation was carried out in a large farrow-to-finish farm producing 40 000 fatteners per year. In the spring of 2000, growth retardation, wasting and respiratory disorders affected 20% of weaners and 30% of fatteners. Nineteen weaners and 11 fatteners with different degrees of wasting, respiratory disorders, skin paleness and hemorrhagic skin lesions (fatteners only) were necropsied. Post-mortem examination revealed the presence of inflammatory signs in lymphoid tissues, enlarged lymph nodes, non-collapsed lungs, enlarged livers, enlarged and pale kidneys with generalized cortical petechiae. Sections of lymph nodes were submitted for histopathological examination and in situ hybridization. Moreover, virus isolation in PK15 cell line was attempted. Histopathological analysis of lymph nodes revealed different degrees of lymphatic depletion and histiocitic infiltrations, giant cells and intracytoplasmic inclusion bodies. In situ hybridization with the use of a specific for PCV2 digoxigenin labelled DNA probe confirmed the abundant presence of PCV2 DNA within lesions in lymph nodes. A peroxidase assay indicated PCV2 replication in the inoculated cell line. Gross and histopathological lesions and the presence of PCV2 antigens in lymphoid tissues confirmed the presence of PMWS in a Polish swine herd. Clinical signs and laboratory examinations indicated that PDNS and PMWS coexisted in the herd. This suggests that PCV2 could be an important factor in an aetiology of PDNS.

Techniki bioobrazowania na poziomie komórkowym metody in situ - cz. I

84%

Delenko K. , Hyjek M. , Kozlowska M. , Sadowski M. , Kolowerzo A. , Bednarska-Kozakiewicz E.

nr 3

Metody laboratoryjnego rozpoznawania zespolu rozrodczo-oddechowego swin [PRRS] i interpretacja ich wynikow

67%

Stadejek T.

2002

tom Supl.Swinie

33-37

Zastosowanie genomowej hybrydyzacji in situ do identyfikacji chromosomow u mieszancow oddalonych traw

67%

Zwierzykowski Z. , Naganowska B. , Lesniewska A.

1998

tom 05

137-138