The objective of this study was to compare the efficacy of human chorionic gonadotropin (hCG) for the stimulation of ovulation within 48 h after treatment in mares, which had a ≥35 mm follicle. A total of 41 oestrus cycles of 21 mares (9 Thoroughbred, 6 Dutch Warmblood, and 6 Friesian), between 7 and 12 years of age were used for this study. All the mares were intramuscularly treated with Hcg, at the dose of 3 000 IU when a follicle ≥35 mm was detected. They were examined daily by transrectal ultrasonography, until the ovulation was confirmed. It was found that the diameter of follicle before ovulation was more significant, and oestrus duration was longer in Friesian mares than those of Thoroughbred and Dutch Warmblood ones. Moreover, the percentage of mares ovulating within 48 h after hCG treatment was significantly lower in Friesian mares, when compared to that of the other breed mares. There was no significant difference in these parameters between Thoroughbred and Dutch Warmblood mares. In conclusion, the efficacy of hCG may be different among mares of different breeds. The percentage of ovulation within 48 h after hCG treatment was lower in Friesian mares when compared to the other breeds. Therefore, various strategies are needed for the stimulation of ovulation in Friesian mares.
Striated murrel Channa striatus were injected with natural hormones (pituitary extract and human chorionic gonadotropin) and synthetic hormones (luteinizing hormone releasing hormone analogue and ovaprim). When compared to the LHRHap and ovaprim, the latency period was long in pituitary- (24 h) and HCG-injected (26 h) fish. In the pituitary-injected C. striatus the percentage of fertilisation was the lowest (60-68%) but the duration of hatching was longest (39-43 h) followed by HCG- (36-38 h), LHRHap- (34-36 h) and ovaprim-injected (21-23 h) individuals. In terms of fertilisation (95-98%) and hatching, ovaprim yielded better results. Ova reached the highest diameter (1.34-1.45 mm) in C. striatus injected with ovaprim, followed by HCG (1.22-1.30 mm) and pituitary (1.21-1.27 mm). The lowest ova diameter (1.07-1.09 mm) was observed in C. striatus injected with LHRHap.
PL
Zmijogłowom indyjskim, Channa striatus wstrzyknięto naturalne (ekstrakt z przysadki mózgowej, HGC - ludzką gonadotropinę kosmówkową) oraz syntetyczne hormony (LHRHap - analog podwzgórzowego hormonu uwalniającego hormon luteinizujący i ovaprim®). Czas reakcji - w porównaniu do LHRHap i ovaprimu - był względnie długi w przypadku stosowania ekstraktu z przysadki (24 h) i ludzkiej gonadotropiny kosmówkowej (26 h). U ryb, którym wstrzyknięto ekstrakt przysadki, procent zapłodnienia był najniższy (60-68%). Dla odmiany, wylęganie u potomstwa tych ryb trwało najdłużej (39-43 h). Na kolejnych miejscach były ryby poddane działaniu HCG (36-38 h), LHRHap (34-36 h) i ovaprimu (21—23 h). Pod względem zapłodnienia i wylęgania ovaprim dał lepsze wyniki. Największą średnicę (1,34-1,45 mm) osiągnęły jaja C. striatus stymulowanych ovaprimem. Nieco mniejsze były jaja w przypadku ryb pod działaniem HCG (1,22-1,30 mm) i przysadki (1,21-1,27 mm). Najmniejsza średnica jaj (1,07-1,09 mm) była obserwowana u C. striatus, którym wstrzyknięto LHRHap.
Five combinations of human chorionic gonadotropin (hCG) dose and five latency periods were evaluated to observe the pattern of deformed larvae among the hatchlings in walking catfish, Clarias batrachus (L.). A higher percentage (7%–12%) of deformed larvae were observed at 1000, 2000, and 5000 IU · kg-1 with 14–23 h latency combinations. The results of the study indicated that 3000 IU · kg-1 with 14–23 h and 4000 IU · kg-1 with 14–17 h latency combinations were preferable to reduce the deformed larvae (4%–6%) among the hatchlings during induced spawning of the catfish.