Wyniki wyszukiwania - Biblioteka Nauki

1

Influence of 4-hydroxynonenal and spleen cells on primary hepatocyte culture and a novel liver-derived cell line resembling hepatocyte stem cells

100%

Cipak A. , Borovic S. , Jaganjac M. , Bresgen N. , Kirac I. , Grbesa I. , Mrakovcic L. , Cindric M. , Scukanec-Spoljar M. , Gall-Troselj K. , Coric M. , Eckl P. , Zarkovic N.

Acta Biochimica Polonica

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2010

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tom 57

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nr 2

185-191

EN

Liver is a unique mammalian organ with a great capacity of regeneration related to its function. After surgical resection or injury, hepatic cells, especially hepatocytes, can proliferate rapidly to repair the damage and to regenerate the structure without affecting the function of the liver. Loss of catalase activity during regeneration indicates that oxidative stress is present in the liver not only in pathological conditions but also as a 'physiological' factor during regeneration. As we have shown in our previous work, liver stem cell-like cells treated with 4-hydroxynonenal (HNE), a cytotoxic and growth regulating lipid peroxidation product, recover in the presence of spleen cells. In the current study we characterized this novel cell line as liver-derived progenitor/oval-like cells, (LDP/OCs), i.e. functional liver stem-like cells. We showed that LDP/OC were OV6 positive, with abundant glycogen content in the cytoplasm and expressed α-fetoprotein, albumin, biliverdin reductase and γ-glutamyl transferase. Also, we compared their growth in vitro with the growth of cultured primary hepatocytes stressed with HNE and co-cultured with autologous spleen cells. The influence of spleen cells on HNE-treated primary hepatocytes and on LDP/OCs showed that spleen cells support in a similar manner the recovery of both types of liver cells indicating their important role in regeneration. Hence, LDP/OC cells may provide a valuable tool to study cell interactions and the role on HNE in liver regeneration.

2

Ultrastructural study of hepatocytes in the experimental model of acute pancreatitis

100%

Romanowska-Sarlej J. , Kifer-Wysocka E. , Krolikowska-Prasal I. , Jodlowska-Jedrych B. , Matysiak W.

Folia Histochemica et Cytobiologica

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1999

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tom 37

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nr 2

3

Cytoprotective action of 17beta-oestradiol against iron-induced hepatic oxidative stress in vitro

75%

Wojcik M. , Kosior-Korzecka U. , Bobowiec R.

Bulletin of the Veterinary Institute in Puławy

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2010

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tom 54

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nr 2

259-263

EN

The objective of this study was to analyse the response of hepatocytes on various concentrations of 17ß-oestradiol (17ß-E) under iron-induced oxidative stress in vitro. Isolated by in situ collagenase perfusion hepatocytes were cultured in DMEM/HAMS-12 (v/v) medium without any additional agents (control), with Fe³⁺ alone, and with Fe³⁺ aild 0.2%, 0.02%, and 0.002% solution of 17ß-E (17ß-EI, 17ß-EII, and 17ß-EIII, respectively). After 24, 48, and 72 h, medium malonylodialdehyde (MDA), haptoglobin (Hpt) concentration and proliferative activity were determined. In comparison to control samples, and samples collected at 24 and 72 h, hepatocytes exposition to Fe³⁺, caused a significant increase in MDA (0.056 ±0.011 nM/mL) only after 48 h of incubation. Each of 17ß-E concentrations resulted in a decrease in MDA in samples obtained after 24 and 48 h. In comparison to the first 24 h, Fe³⁺ alone and together with 17ß-EI, 17ß-EII, and 17ß-EIII caused a significant augmentation of Hpt level in 48 h and 72 h of the experiment. Each of the 17ß-E concentrations added to the culture medium resulted in inhibition of hepatic proliferative activity, especially in the 72 h of cell culture.

4

Inhibition of soluble epoxide hydrolase offers protection against fructose-induced diabetes and related metabolic complications in rats

75%

Shah S. A. , Mehmood M. H. , Khan M. , Bukhari I. A. , Alorainey B. I. , Vohra F.

Journal of Physiology and Pharmacology

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2020

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tom 71

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nr 5

5

Development of hepatocytes in nase (Chondrostoma nasus (L.)) larvae following hatch

75%

Ostaszewska T. , Sysa P.

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tom 12

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nr 2

EN

The development of the nase liver was examined under light and electron microscopes from the moment of hatching until the juvenile stage. Three phases of hepatocyte differentiation were observed during the organogenesis of nase livers. In the first phase, from hatching until day 4, the hepatoblasts of the primordial liver are morphologically undifferentiated and divided by sinus vessels. They also store glycogen. In the second phase, from the moment when the mouth cavity becomes passable until the resorption of the yolk sac, organelles typical of the structure of hepatocytes appear and begin to function. At the end of this phase signs of bile lipid synthesis and secretion become visible. The third phase is when exogenous nutrition begins and is characterized by the increased activity of the significant organelles engaged in protein synthesis and secretion, such as the endoplasmic reticulum and the Golgi apparatus.

PL

Na podstawie obserwacji histologicznych i ultrastrukturalnych stwierdzono, że rozwój hepatocytów świnki ma podobny przebieg jak u innych gatunków ryb kostnoszkieletowych. Rozwój hepatocytów świnki można podzielić na trzy okresy obejmujące kolejne fazy odżywiania się. Okres endotroficznego odżywiania, podczas którego rezerwy pęcherzyka żółtkowego są przetwarzane w syncytium okołożółtkowym i rozprowadzane przez system krążenia. Wówczas hepatocyty różnicują się, pojawiają się zapasy glikogenu i niewielka ilość lipoprotein (fot. 1, 2, 3). Drugi okres endo-egzotroficzny rozpoczyna się udrożnieniem jamy ustnej, pobraniem pierwszego pokarmu, zwiększeniem aktywności aparatu Golgiego i wytwarzaniem lipidów żółciowych (fot. 4, 5). Trzeci okres to wyłącznie odżywianie egzogenne, w wątrobie magazynowane są i odtwarzane zapasy glikogenu oraz lipidów (fot. 6, 7).

6

Immunohistochemical evaluation of caspase 3 expression in rats' hepatocytes after L-arginine therapy

75%

Pedrycz A. , Kot K. , Olesinski I.

Bulletin of the Veterinary Institute in Puławy

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2010

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tom 54

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nr 1

101-103

EN

The apoptotical effect of nitric oxide on effector apoptotical caspase 3 in rats' hepatocytes was examined. The experiment was performed on 16 white Wistar female rats divided into two equal groups. The rats from the experimental group received orally L-arginine in a dose of 40 mg/kg b.w. every other day for 2 weeks. The rats from the control group received orally 2 ml of distilled water in the same manner as the experimental group. All the rats were decapitated after 3 weeks of the experiment. After decapitation, specimens from the liver were collected, fixed in 10% formalin, and then embedded in paraffin blocks. Protein caspase 3 on slides was detected using the standard three-step immunohistochemical method. The quantitative evaluation of caspase 3 expression showed that the area occupied by positive caspase 3 reaction in the liver of the experimental group (128.11 µm²±96.54) was comparable to that in the control group (212.18 µm² ±1 16.59) (P=0.25). The dose of L-arginine used was similar to that applied in pregnant women treated for gestosis. The study shows that L-arginine as a donor of exogenous nitric oxide has no an apoptotic effect on rats' hepatocytes.

7

Hepatic complications in acute pancreatitis

75%

Dlugosz J. W.

Journal of Physiology and Pharmacology. Supplement

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1998

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tom 49

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nr 2

8

Hypothermic storage of equine isolated hepatocytes

75%

Bakala A. , Karlik W. , Wiechetek M.

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tom 10

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nr 1

EN

The aim of the study was to establish the optimal methods for hypothermic storage of equine isolated hepatocytes. Viability of equine isolated hepatocytes after hypothermic storage was dependent on the type of storage medium as well as on the cell density in the storage suspension and the preservation period. Hepatocytes stored at 4°C in Hanks' Balanced Salt Solution (HBSS) and Williams' Medium E (WE) for 24 h showed very low viability, numerous cell membrane blebs, very low attachment rate (11.9 ± 6.5% and 34.8 ± 19.1%, respectively) and 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) reduction rate (6.4 ± 3.9% and 25.1 ± 14.8%, respectively). In contrast, hepatocytes stored in University of Wisconsin Solution (UW) after 24 h of storage at a density of 12.5 x 106 cells/ml showed high viability (over 70%), typical and intact morphology, high cell attachment rates and MTT reduction. Our findings clearly demonstrate that UW is a good preservation solution for equine isolated hepatocytes. Hepatocytes harvested from slaughterhouse organs can be stored at 4°C in UW at a density of 12.5 x 106 cells/ml for at least 24 h without significant decrease in functional integrity.

9

Morphometric analysis of argyrophylia

75%

Tosik D. , Orkisz S. , Bartel H.

Folia Histochemica et Cytobiologica. Supplement

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1996

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tom 34

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nr 1

10

Nuclear bodies in hepatocytes of the rat

75%

Orkisz S. , Bartel H. , Tosik D.

Folia Histochemica et Cytobiologica. Supplement

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1996

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tom 34

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nr 1

11

Isolation of hemoglobin receptor on hepatocytes

75%

Zuwala-Jagiello J. , Osada J.

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1994

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tom 12

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nr 2

12

Effect of protein tyrosine kinases on mitochondrial calcium homeostasis in rat hepatocytes treated with thapsigargin

75%

Walajtys-Rode E. , Moehren G. , Hoek J. B.

Cellular and Molecular Biology Letters

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1998

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tom 03

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nr 3

13

Vincristine-induced autophagy in mouse liver

75%

Krol T.

Acta Biologica Cracoviensia. Series Zoologia

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1996

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tom 38

14

Short-term fenofibrate treatment improves ultrastructure of hepatocytes of old rats

63%

Zubrzycki A. , Wronska A. , Zauszkiewicz-Pawlak A. , Kmiec Z.

Folia Histochemica et Cytobiologica

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2021

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tom 59

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nr 3

15

Dysregulation of gene expression in ABCC6 knockdown HepG2 cells

63%

Miglionico R. , Armentano M. F. , Carmosino M. , Salvia A. M. , Cuviello F. , Bisaccia F. , Ostuni A.

Cellular and Molecular Biology Letters

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2014

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tom 19

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nr 4

EN

ABCC6 protein is an ATP-dependent transporter that is mainly found in the basolateral plasma membrane of hepatocytes. ABCC6 deficiency is the primary cause of several forms of ectopic mineralization syndrome. Mutations in the human ABCC6 gene cause pseudoxanthoma elasticum (PXE), an autosomal recessive disease characterized by ectopic calcification of the elastic fibers in dermal, ocular and vascular tissues. Mutations in the mouse ABCC6 gene were also associated with dystrophic cardiac calcification. Reduced levels of ABCC6 protein were found in a β-thalassemic mouse model. Moreover, some cases of generalized arterial calcification in infancy are due to ABCC6 mutations. In order to study the role of ABCC6 in the pathogenesis of ectopic mineralization, the expressions of genes involved in this process were evaluated in HepG2 cells upon stable knockdown of ABCC6 by small hairpin RNA (shRNA) technology. ABCC6 knockdown in HepG2 cells causes a significant upregulation of the genes promoting mineralization, such as TNAP, and a parallel downregulation of genes with anti-mineralization activity, such as NT5E, Fetuin A and Osteopontin. Although the absence of ABCC6 has been already associated with ectopic mineralization syndromes, this study is the first to show a direct relationship between reduced ABCC6 levels and the expression of pro-mineralization genes in hepatocytes.

16

In vitro and in vivo effects of magnesium on the lysosomal system

63%

Kopacz-Bednarska A. , Krol T. , Trybus E. , Trybus W. , Zaporowska H. , Kolaczek K. , Karpowicz E.

Folia Biologica

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2018

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tom 66

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nr 4

17

Optimization of the technique for the short-term culture of equine hepatocytes

63%

Bakala A. , Karlik W. , Wiechetek M. , Grono D. , Glowala A.

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tom 63

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nr 11 Supl.

1440-1442

EN

The aim of this study was to establish the optimal conditions for the culture of equine hepatocytes in a monolayer configuration. The obtained results show that the rate of MTT metabolism correlated with the number of cultured cells and a linear increase of MTT reduction rate was observed in cases when the cell density varied between 1.25 × 10⁴ to 5 × 10⁴ viable cell/well of 96-well plate. Hepatocytes reached the optimal cell attachment rate and MTT reduction at a cell density of 5 × 10⁴ cells/well. The number of attached cells to a plastic culture dish was also related to incubation time. The greatest ability of hepatocytes to attach to the culture dish was observed after 10 h of incubation and it was found to be 84.1 ± 2.5% of seeded hepatocytes. It was also found that fetal bovine serum was more efficient than horse serum for the attachment of equine isolated hepatocytes in a monolayer culture. The highest rate of cell attachment (assessed microscopically and with MTT reduction test) was observed when cells were plated with the culture medium supplemented with FBS or HS at a concentration of 5%. However, medium supplementation with higher than 5% serum concentration (10% of FBS or HS) significantly decreased MTT reduction rate. The rate of MTT metabolism and cell attachment in hepatocytes cultured in WE supplemented with FBS or HS was also dependent on the plating time and were the highest after 10 h of seeding.

18

Immunolocalization in portal acinus of the liver of different types of adriamycin induced cell death depending on time

63%

Pedrycz A. , Boratynski Z. , Mendocha J.

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nr 02

EN

The ability to control the phenomenon of apoptosis, its induction or inhibition, raises hopes for treating many diseases including cancer. Adriamycin, an antibiotic that is wildly used after treating cancer, induces apoptosis in liver cells in a certain and relatively quick way after its application. The aim of the work was to obtain and examine the model of apoptosis and necrosis of hepatocytes with respect to their response to different damaging stimuli (adriamycin) depending on the time after the application in correlation with the ultrastructural construction, which is the result of the different location of hepatocytes within the portal acinus (of Rappaport). There were 32 female white Wistar rats used in the study. They were divided into 4 groups (2 experimental and 2 control), 8 animals in each group. The adriamycin dose of 5 mg/kg was administered intraperitoneally to the rats in groups I and II and then the rats were decapitated after 4 weeks (group I) and after 8 weeks (group II). The rats in the control groups were given 0.5 ml 0.9% NaCl solution and then decapitated after 4 weeks (group III) and 8 weeks (group IV). In the research, preparations made from fragments of the right liver lobe were used for histological observations and immunohistochemical studies. In the immunohistochemical studies, a three-stage method was used. According to this method, hepatocytes were examined qualitatively and quantitatively for the presence of proteins involved in apoptosis, to which the death signals run: through mitochondrial pathways (caspase 3 and caspase 9), through intrinsic pathways by endoplasmic reticulum (caspase 3 and caspase 12), through extrincic pathways (caspase 3 and caspase 8) and one from inflammatory markers: caspase 1. Histological images showed that the apoptosis phenomenon occurs after the administration of adriamycin in hepatocytes in a zonate way and is dependent on the time that has elapsed since its administration. Immunohistochemical studies showed, in both a qualitative and quantitative way, a phenomenon of apoptosis in hepatocytes (executive caspase 3) and necrosis (caspase 1). It was also proved that the signal for the induction of apoptosis showed zonation and mainly followed the mitochondrial pathway (caspase 9); the intrinsic pathway by endoplasmic reticulum was much less common (caspase 12); while even more rarely caspase 8 was identified as a marker of an extrinsic pathway to induce apoptosis.

19

Effect of hyperthermic shock on RNP structures in isolated RAT hepatocytes

63%

Orkisz S. , Puvion E.

Folia Histochemica et Cytobiologica

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1984

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tom 22

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nr 2

20