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1 Bulletin of the Veterinary Institute in Puławy

1 2001

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Phenotypic characteristics of Pasteurella multocida strains isolated from cattle affected with haemorrhagic septicaemia

100%

Kedrak A. , Borkowska-Opacka B.

nr 2

Biochemical properties, serotype classification and drug resistance were evaluated in 41 Pasteurella multocida strains isolated from cattle suffering from haemorrhagic septicaemia (HS). The API 20E test and standard methods were used to examine selected biochemical features of the strains. The results permitted classifying the isolates as P. multocida subspecies multocida strains. All the isolates revealed both capsular antigen B (by Carter) and the somatic antigen specific to the serotype 2 (by Heddleston). A weak positive reaction with the specific serum to serotype 5 was noticed. Haemorrhagic septicaemia was serologically confirmed for the first time in Poland. The isolates examined were highly sensitive to antibiotics and chemiotherapeutics. A distinct resistance was found in the case of streptomycin; several strains were also resistant to neomycin, gentamycin, erythromycin or sulphonamides.