Wyniki wyszukiwania - Biblioteka Nauki

1

Hematological and antifungal properties of temporin A and a cecropin A-temporin A hybrid.

100%

Wade D. , Silveira A. , Rollins-Smith L. , Bergman T. , Silberring J. , Lankinen H.

Acta Biochimica Polonica

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2001

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tom 48

|

nr 4

1185-1189

EN

Temporin A (TA) and a cecropin A-temporin A hybrid peptide (CATA) were synthesized and assayed for their hemolytic, anticoagulant, and antifungal properties. CATA retains significant antifungal activity, is less hemolytic than TA, and inhibits blood coagulation. These results recommend further studies of the biological activities of CATA.

2

The effect of sodium taurodeoxycholate on human erythrocytes

100%

Mrowczynska L. , Bielawski J.

Cellular and Molecular Biology Letters

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1998

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tom 03

|

nr 2

3

Emulsions of oil from Adenanthera pavonina L. seeds and their protective effect

100%

Jaromin A. , Zarnowski R. , Kozubek A.

|

nr 3

EN

In our previous study, we developed very stable formulations of submicron oil-in-water emulsions from Adenanthera pavonina L. (family Leguminosae, subfamily Mimosoideae) seed oil, stabilised with soybean lecithin (SPC). Continuing our research, we introduced an additional co-emulsifier, Tween 80, to those formulations in order to decrease the size of the emulsion particles and improve their stability. Formulations with a mean particle size ranging from 43.6 to 306.5 nm and a negative surface charge from −45.3 to −28.5 mV were obtained. Our stability experiments also revealed that most of the tested formulations had a very good degree of stability over a 3-month storage period, both at 4°C and at room temperature. Since many intravenous injectable drugs exhibit lytic activity against erythrocytes, we examined this activity for the emulsion form of cardol, a natural compound with already proven hemolytic properties. The incorporation of this agent into the emulsion caused an evident decrease in hemolytic activity (97–99%). This highly protective effect, observed against sheep erythrocytes, was independent of both the composition and the particle size of the emulsions used. Our studies suggest that nonionic surfactant/phospholipid-based emulsions containing this edible oil of A. pavonina L. may be useful as an alternative formulation matrix for pharmaceutical, nutritional or cosmetic applications of otherwise membrane-acting components.

4

Effects of low doses of gamma rays on the stability of normal and diabetic erythrocytes

86%

Kaczmarska M. , Kopyściańska Z. , Fornal M. , Grodzicki T. , Matlak K. , Korecki J. , Burda K.

Acta Biochimica Polonica

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2011

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tom 58

|

nr 4

489-496

EN

We studied the influence of low doses of γ radiation (from 0.04 to 1.8 mGy) on the stability of human red blood cells (RBC) from healthy donors and diabetic patients using absorption spectroscopy. Because of the alteration of many enzymatic pathways in diabetic RBCs resulting in strong modification of the lipid and protein membrane components one could expect that the ionizing γ-radiation should influence the stability of the healthy and diabetic cells in a different way. Indeed, distinct discontinuities and monotonic changes of hemolysis detected in the healthy and diabetic RBCs suggest that various enzymatic and chemical processes are activated in these membranes by γ radiation. Mössbauer measurements showed that only the highest applied dose of γ radiation caused modification of hemoglobin in both types of RBCs.

5

Japanese-made implantable centrifugal type ventricular assist system (LVAS): EVAHEART

86%

Umezu M. , Yamazaki K. , Yamazaki S. , Iwasaki K. , Miyakoshi T. , Kitano T. , Tokuno T.

Biocybernetics and Biomedical Engineering

|

2007

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tom Vol. 27, no. 1-2

111-119

EN

Due to a shortage of donor heart in Japan, for end-stage congestive heart failure patients a demand to provide a better quality of life with the left ventricular assist system (LVAS) is strong. EVAHEART LAVAS uses a Japanese-made compact centrifugal pump, which can be implanted into a narrow thoracic cavity. This work has been a multi-institutional project carried out in collaboration with over 50 industrial companies in various technical disciplines, and it was financially supported mainly by a private fund as well as the Japan Science and Technology corporation (JST) fund. And the University of Pittsburgh Medical Center has contributed to the animal model evaluation since 1993. The pump flow performance was represented as head-flow (H-Q) relations, demonstrating that a blood pump flow of 8 l/min can be achieved at 100 mmHg at the pump speed of 2400 rpm. This performance should be sufficient as a clinical LVAS under most of practical conditions. The in vitro durability test was performed as a system as well as components. The results show a sufficient durability and reliability. A favorable biocompatibility with the blood contacting surface was confirmed by a series of animal experiments, which include eight long-term evaluations (90-180 days) in calves, with no serious problem such as thrombus formation or mechanical problems in recent cases. Various tests with the risk conditions have also been conducted, one of which is the hemolysis test with simulated seal defect conditions. The first clinical implantation of EVAHEART was conducted at Tokyo Women’s Medical University in May, 2005. Up to now, all three patients are in satisfactory hearth conditions.

6

Induction of hemolysis and eryptosis by occupational pollutant nickel chloride is mediated through calcium influx and p38 MAP kinase signaling

86%

Alfhili M. A. , Alamri H. S. , Alsughayyir J. , Basudan A. M.

International Journal of Occupational Medicine and Environmental Health

|

2021

|

tom 35

|

nr 1

1-11

EN

ObjectivesNickel (Ni) is an abundant environmental hazard and an occupational pollutant. Exposure to Ni compounds is prevalent in electroplating workers and in the printing industry, among others. The toxicity of Ni manifests as dermatological, gastrointestinal, respiratory, allergic, and cardiovascular symptoms. In particular, hyperbilirubinemia and reticulocytosis have been detected in intoxicated subjects; an observation possibly implicating selective red blood cell (RBC) toxicity. Herein, the interaction of nickel chloride (NiCl2) with human RBCs and associated molecular mechanisms are described.Material and MethodsCells from healthy donors were incubated for 24 h at 37°C in the presence or absence of 0.5‒10 mM of NiCl2, and cytotoxicity was determined through hemoglobin leakage by colorimetry under different experimental conditions. Eryptotic markers were also identified by flow cytofluorometry using Annexin-V-FITC tagging for phosphatidylserine (PS) exposure, light scatter properties for cellular dimensions, Fluo4/AM labeling for intracellular calcium, and H2DCFDA staining for reactive oxygen species (ROS). Additionally, small molecule inhibitors were used to probe the signaling pathways involved.ResultsIt was found that NiCl2 at 10 mM caused profound intracellular calcium overload and significant calcium-dependent hemolysis. Also, NiCl2 reduced forward scatter and increased side scatter, Annexin-positive cells, and ROS levels. Importantly, NiCl2-induced hemolysis was significantly attenuated by the exclusion of extracellular calcium, and in the presence of p38 MAP kinase (MAPK) inhibitor SB203580.ConclusionsIt is concluded that NiCl2 induces p38 MAPK-dependent hemolysis, and stimulates the canonical features of premature eryptosis. This report presents the first description of the molecular mechanisms underlying the hemolytic and eryptotic potential of NiCl2 and, thus, may explain changes in hematological parameters observed in poisoning victims.

7

Effects of L-carnitine on morphology and cellular parameters of hen erythrocytes

86%

Kasperek K. , Bownik A. , Knaga S. , Szabelak A. , Ślaska B. , Kwiecień M. , Jeżewska-Witkowska G. , Kasperek K. , Bownik A. , Knaga S. , Szabelak A. , Ślaska B. , Kwiecień M. , Jeżewska-Witkowska G.

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nr 4

8

Hemoliza jako podstawowy problem przedanalityczny

86%

Sztefko K. , Szybowska P.

Laboratorium - Przegląd Ogólnopolski

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2013

|

tom nr 5-6

38--39

PL

Hemoliza jest najczęstszym i najbardziej znanym czynnikiem przedanalitycznym wpływającym na wyniki oznaczeń wykonywanych w laboratoriach analitycznych. Hemoliza egzogenna może być wynikiem niewłaściwego pobrania, transportu lub postępowania z próbką. Fałszywie zawyżony lub zaniżony wynik oznaczenia z powodu interferencji pochodzącej od hemolizy zwiększa ryzyko błędnej diagnozy pacjenta.

EN

Hemolysis is the most frequent and well-known preanalitycal factor influencing the results of many laboratory tests. Exogenous hemolysis results from inappropriate blood sample collection and transportation as well as from wrong handling of the specimens. Falsely elevated or falsely decreased results of the laboratory measurement due to hemolysis increase the risk of an incorrect diagnosis of the patient.

9

The in vitro hemolytic activity of erythrocytes from free-living mute swan (Cygnus olor) spread out in Gdynia and Sopot Coasts

86%

Tkachenko H. , Kurhaluk N. , Hetmanski T.

Baltic Coastal Zone. Journal of Ecology and Protection of the Coastline

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2018

|

tom 22

EN

The erythrocytes could be considered as a valuable model in studying the cytotoxic effect of environmental xenobiotics owing to its particular structure and the important components of their plasma membrane especially lipids and proteins. The aim of our study was to investigate the erythrocyte hemolytic activity of mute swan (Cygnus olor) from Baltic coastal region in two points, i.e. Sopot and Gdynia. These comparisons will show whether mute swans on the Baltic coastal regions have elevated levels of hemolysis plus gender variation in erythrocyte damage. Forty adult mute swans were captured from Sopot and Gdynia in February 2013. Collected birds were measured and weighted, and the blood was sampled. The birds were divided into 4 groups (after the 2nd/3rd year of life): males from Sopot (n = 10), females birds from Sopot (n = 10), males from Gdynia (n = 10), and females from Gdynia (n = 10). Blood samples were collected from the medial metatarsal vein in sodium citrate for erythrocyte sedimentation rate. Blood was stored in tubes and held on the ice until centrifugation. The plasma was removed. The erythrocyte suspension (one volume) was washed with five volumes of saline solution three times. The acid resistance of erythrocytes was induced by 0.1M HCl, while osmotic resistance was induced by incubation erythrocyte suspension with the different solution of urea and NaCl, as well as in solutions of different NaCl concentration (0.1-0.9%). The erythrocyte hemolysis was measured spectrophotometrically. Our study revealed that the highest level of the hemolysis percentage was observed among females from Gdynia, males from Sopot, then females from Sopot, and males from Gdynia. The significant differences between the hemolysis percentage among females from Gdynia and Sopot from 13.5 to 18.0 min after HCl-induced hemolysis was observed. The results also indicated significant differences between the hemolysis percentage among males from Gdynia and Sopot from 14.0 to 19.0 min. Hemolysis percentage showed to be increased not significantly among males from Gdynia with time from hemolysis initiation to 10.0 min but close to linearity with a value of hemolysis percentage among males from Sopot after 25 min of hemolysis initiation. The maximal level of hemolysis percentage was significantly higher among females from Gdynia (at 15.0 min) compared to females from Sopot (at 14.0 min). The maximal level of hemolysis percentage among males from Gdynia and Sopot was noted at 0.5 min after hemolysis initiation. The maximal level of hemolysis percentage was 4.40% and 4.11%, respectively. The highest percentage of hemolyzed erythrocytes after incubation with solutions of various urea concentration was observed both among males and females from Gdynia. The lowest percentage of hemolyzed erythrocytes was exhibited by the erythrocyte suspension of males from Sopot. Comparing the percentage of hemolyzed erythrocytes both among males and females from Sopot, the higher values of hemolyzed erythrocytes was noted among females. The highest percentage of hemolyzed erythrocytes after incubation with solutions of various NaCl concentration was observed among males and females from Sopot and further among males and females from Gdynia after incubation with 0.5-0.9% NaCl solution. Comparing the percentage of hemolyzed erythrocytes after incubition with 0.1-0.4% NaCl solution, the highest percentage of hemolyzed erythrocytes was observed among males and females from Gdynia and then among males and females from Sopot. Our study showed more individual variation, probably related to the impact of the environmental habitat and consumption of ingested prey items by mute swans. Of those males and females, females from Gdynia showed the highest level of hemolyzed erythrocytes.

PL

Erytrocyty można uznać za cenny model do badania cytotoksycznego działania środowiskowych ksenobiotyków ze względu na ich szczególną strukturę i ważne składniki ich błony komórkowej, zwłaszcza lipidy i białka. Celem naszych badań była ocena aktywności hemolitycznej erytrocytów łabędzia niemego (Cygnus olor) z regionu morza Bałtyckiego (plaże w Sopocie i Gdyni). Porównania te miały pokazać, czy populacja łabędzia niemego w nadbałtyckich regionach przybrzeżnych ma podwyższoną hemolizę erytrocytową oraz czy te zmiany zależą od płci ptaków. Czterdzieści dorosłych łabędzi niemych przebadano w Sopocie i Gdyni w lutym 2013 r. Ptaki podzielono na 4 grupy (po II/III roku życia): samce z Sopotu (n = 10), samice z Sopotu (n = 10), samce z Gdyni (n = 10) i samice z Gdyni (n = 10). Próbki krwi pobierano z przyśrodkowej żyły skoku w probówki z cytrynianem sodu. Krew przechowywano w probówkach i trzymano na lodzie aż do odwirowania. Osocze zostało usunięte. Zawiesinę erytrocytów (jedna objętość) przemyto pięcioma objętościami roztworu soli fizjologicznej trzykrotnie. Kwasową odporność erytrocytów indukowano 0,1M HCl, podczas gdy odporność osmotyczną indukowano przez inkubację zawiesiny erytrocytów w różnych roztworach mocznika i NaCl, a także w roztworach o różnym stężeniu NaCl (0,1-0,9%). Hemolizę erytrocytów mierzono spektrofotometrycznie. Najwyższy poziom hemolizy zanotowano u samic z Gdyni, samców z Sopotu, następnie samic z Sopotu i samców z Gdyni. Stwierdzono istotne różnice między odsetkiem hemolizy u samic z Gdyni i Sopotu z 13,5 do 18,0 min po hemolizie indukowanej HCl. Wyniki wskazują również na istotne różnice pomiędzy odsetkiem hemolizy wśród samców z Gdyni i Sopotu od 14,0 do 19,0 min indukowanej hemolizy. Procent hemolizy zwiększył się nieznacznie u samców z Gdyni w czasie od rozpoczęcia hemolizy do 10 min. Maksymalny odsetek hemolizy był istotnie wyższy wśród samic z Gdyni (po 15,0 min) w porównaniu z samicami z Sopotu (14,0 min). Maksymalny poziom hemolizy wśród samców z Gdyni i Sopotu zanotowano w 0,5 minuty po inicjacji hemolizy. Maksymalny poziom hemolizy wynosił odpowiednio 4,40% i 4,11%. Najwyższy odsetek hemolizowanych erytrocytów po inkubacji z roztworami o różnym stężeniu mocznika obserwowano zarówno u samców, jak i u samic z Gdyni. Najmniejszy odsetek hemolizowanych erytrocytów wykazywała zawiesina erytrocytów samców z Sopotu. Porównując odsetek hemolizowanych erytrocytów zarówno u samców, jak i samic z Sopotu, wyższe wartości hemolizowanych erytrocytów odnotowano wśród samic. Najwyższy odsetek hemolizowanych erytrocytów po inkubacji z roztworami o różnym stężeniu NaCl zaobserwowano u samców i samic z Sopotu, a następnie u samców i samic z Gdyni po inkubacji z 0,5-0,9% roztworem NaCl. Porównując odsetek hemolizowanych erytrocytów po inkubacji z 0,1-0,4% roztworem NaCl, najwyższy odsetek hemolizowanych erytrocytów zaobserwowano u samców i samic z Gdyni, a następnie u samców i samic z Sopotu. Nasze badanie wykazuje większą zmienność osobniczą, prawdopodobnie związaną z oddziaływaniem środowiska naturalnego i spożyciem połkniętych przedmiotów przez nieme łabędzie na plażach Trójmiasta. Spośród samic i samców łabędzi niemych samice z Gdyni wykazały najwyższy poziom hemolitycznych erytrocytów.

10

The kinetics of hemolysis as an indicator of spherocytosis

86%

Chmura A. , Pazdzior G. , Langner M.

Cellular and Molecular Biology Letters

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2002

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tom 07

|

nr 2

11

Effect of vanadium on rat erythrocytes in vitro

86%

Zaporowska H. , Slotwinska M.

Folia Histochemica et Cytobiologica. Supplement

|

1996

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tom 34

|

nr 1

12

The kinetics of haemolysis of spherocytic erythrocytes

86%

Pazdzior G. , Langner M. , Chmura A. , Boguslawska D. , Heger E. , Chorzalska A. , Sikorski A. F.

Cellular and Molecular Biology Letters

|

2003

|

tom 08

|

nr 3

EN

Spherocytosis is a hereditary disease. It results from mutations in genes that encode proteins participating in the attachment of the membrane skeleton to the plasma membrane bilayer of the erythrocyte. In affected cells, interaction between the spectrin-actin meshwork and integral membrane proteins is altered. This results in the weakening of plasma membrane mechanical resistance and diminishing its elasticity. Since defective cells are prone to mechanical destruction and phagocytosis in the spleen, the fraction of morphologically-altered erythrocytes is rather small; this in turn means such an examination is prone to errors. In this paper, we describe a simple method which could be useful in the identification of red blood cells with altered osmotic properties. The method is based on the measurement of the amount of light scattered by a suspension of the red blood cells, during which cells are exposed to osmotic stress in the stopped-flow regime. The obtained plots are fitted to a mathematical formula, the parameters of which can be used as quantitative indicators of the changes in red blood cells' osmotic features. Two types of spherocytotic samples were examined: those with a proven deficiency in ankyrin and those with a decrease in the band 3 anion transporting protein. The presented data show that this method gives a reliable indication of altered osmotic properties of the spherocytic cells.

13

The hemolytic and physiological activities of mixtures of some phenoxy and organophosphorous herbicides

86%

Kleszczynska H. , Bonarska D. , Bielecki K. , Sarapuk J.

Cellular and Molecular Biology Letters

|

2003

|

tom 08

|

nr 1

EN

Experiments were performed investigating the potential to improve the biological activity of some phenoxy and organophosphorous compounds by using them in binary mixtures. The compounds were: 2,4-dichlorophenoxyacetic acid (1) and its sodium salt (2), dibutyl 1-butylamino-l-cyclohexanephosphonate (3) and diethyl 9-butylamino-9-fluorenephosphonate (4), all widely used as herbicides. There were two test methods: the inhibition of cucumber (Cucumis sativus) growth induced by one single herbicide or by equimolar binary mixtures of herbicides; and, in parallel, the hemolytic efficiency of separate compounds or their mixtures. The hemolytic properties of the compounds were studied as hemolysis is generally a good measure of their toxicity, especially in the case of lipophilic compounds. Pig erythrocytes were used as good models for the determination of toxicity and the kinetics of red blood cell hemolysis. In the plant-based experiments, binary mixtures were found to display additive type toxicity. The compounds’ hemolytic activities were of additive or antagonistic types. In some combinations, the addition of a second component did not change the hemolytic efficiency of the first component, and vice versa.

14

Modulation of 5-N-alkylresorcinol hemolytic properties by divalent cations. Dependence of the effect of cations on alkylresorcinol structure

86%

Stasiuk M. , Kozubek A.

Cellular and Molecular Biology Letters

|

1997

|

tom 02

|

nr 1

EN

It was demonstrated that the process of hemolysis of erythrocytes induced by resorcinolic lipids is inhibited by divalent cations present in the incubation medium. The extent of inhibition is dependent on the nature of both type of the cation and the hemolytic agent. Lysis most susceptible to divalent cations is that induced by short (C15) and medium-chained (C19) 5-n-alkylresorcinols, which also have the highest observed hemolytic potencies. Furthermore, the protection was the stronger the stronger lytic activity was exerted by studied compound. The homologs with the longest side chain studied, although exhibiting the lowest hemolytic activity by themself, were the least susceptible to inhibition of their hemolytic activity by cations. The effect of cobalt cation has been found to be intermediate between the effect of Mn2+ and Zn2+. The most effective in protection of erythrocytes was Zn2+ which almost completely protected the cells against alkylresorcinol-induced lysis at the concentrations of 10-6 M and above.

15

Effect of some lysosomotropic substances on erythrocyte membrane

86%

Kleszczynska H. , Luczynski J. , Witek S. , Przestalski S.

Polish Journal of Environmental Studies

|

1997

|

tom 06

|

nr 6

EN

The effect of N,N-dimethylaminoethyl esters of fatty acids (DM n) on pig red blood cell hemolysis and erythrocyte membrane fluidity has been investigated. In the hemolytic experiments the hemolytic activity of the compounds studied was determined, and was found to increase with alkyl chain length and followed the sequence: DM-15 > DM-13 > DM-11 > DMDA > DM-9. The fluorimetric studies were done using the fluorescent probe TMA-DPH, which allowed us to calculate polarization coefficient P and hence determine relative changes in membrane fluidity induced by the lysosomotropic substances. The compounds of highest hemolytic activity, which have fifteen- and thirteen-carbon-atom alkyl chain (DM-15 and DM-13), significantly affect the erythrocyte membrane fluidity.

16

Modulation of hemolytic properties of resorcinolic lipids by divalent cations

86%

Stasiuk M. , Kozubek A.

Cellular and Molecular Biology Letters

|

1996

|

tom 01

|

nr 2

EN

The effect of divalent cations in concentrations 10-9-10-3M upon the hemolysis of sheep erythrocytes induced by resorcinolic lipids was studied. It was demonstrated that the process of hemolysis is inhibited by divalent cations present in the incubation medium. The extent of inhibition is dependent on both type of the cation and hemolytic agent. In alkylresorcinol-induced hemolysis the most effective in protection of the cells were Zn2+ and Co2+ cations. Weaker protective action was observed for Cd2+ and Mn2+. Calcium ions, in all studied concentrations, were without marked effect on the level of relative hemolysis. Magnesium ions as well as Ba2+ in nanomolar concentrations were effective inhibitors of the lysis but this effect diminished at higher (millimolar) concentrations of these ions. When alkenylresorcinols were used as hemolysis-inducers no effective protection of erythrocytes by Ca2+, Ba2+ or Mg2+ was observed. Most effective in protection of cells against this amphiphile-induced lysis, similarly to their effect observed previously for various lytic agents, were cations of Zn2+, Co2+ and Cd2+. Mn2+ cations were intermediate, with respect to their protective potency.

17

Peroxynitrite-induced hemolysis of human erythrocytes and its possible mechanism

86%

Soszynski M. , Klimczak A. , Grzelak A. , Bartosz G. , Zylinska L.

Cellular and Molecular Biology Letters

|

2002

|

tom 07

|

nr Suppl.

18

Gemini ester quat surfactants and their biological activity

72%

Luczynski J. , Frackowiak R. , Wloch A. , Kleszczynska H. , Witek S.

Cellular and Molecular Biology Letters

|

2013

|

tom 18

|

nr 1

EN

Cationic gemini surfactants are an important class of surface-active compounds that exhibit much higher surface activity than their monomeric counterparts. This type of compound architecture lends itself to the compound being easily adsorbed at interfaces and interacting with the cellular membranes of microorganisms. Conventional cationic surfactants have high chemical stability but poor chemical and biological degradability. One of the main approaches to the design of readily biodegradable and environmentally friendly surfactants involves inserting a bond with limited stability into the surfactant molecule to give a cleavable surfactant. The best-known example of such a compound is the family of ester quats, which are cationic surfactants with a labile ester bond inserted into the molecule. As part of this study, a series of gemini ester quat surfactants were synthesized and assayed for their biological activity. Their hemolytic activity and changes in the fluidity and packing order of the lipid polar heads were used as the measures of their biological activity. A clear correlation between the hemolytic activity of the tested compounds and their alkyl chain length was established. It was found that the compounds with a long hydrocarbon chain showed higher activity. Moreover, the compounds with greater spacing between their alkyl chains were more active. This proves that they incorporate more easily into the lipid bilayer of the erythrocyte membrane and affect its properties to a greater extent. A better understanding of the process of cell lysis by surfactants and of their biological activity may assist in developing surfactants with enhanced selectivity and in widening their range of application.

19

Extended multiple-locus variable-number tandem-repeat analysis of Bacillus anthracis strains isolated in Poland

72%

Gierczynski R. , Jakubczak A. , Jagielski M.

Polish Journal of Microbiology

|

2009

|

tom 58

|

nr 1

3-7

EN

Twenty-one variable-number tandem-repeat (VNTR) marker loci were used for extended multiple locus VNTR analysis (MLVA) of 14 laboratory strains of Bacillus anthracis isolated in Poland and vaccine strain Sterne 34F2A. The extended MLVA (MLVA-21) distinguished six genotypes clustered in three main branches. Monomorphic branch 1 consisted of the vaccine strain and six isolates from distinct samples of a cow died from anthrax. This group also encompassed three haemolytic isolates of B. anthracis. Branches 2 and 3 were heterogeneous and consisted of five and three isolates of the phylogenetic lineages B2 and A1, respectively. MLVA-21 supported thesis on the anthrax agent heterogeneity in Poland. This study brought an additional evidence that haemolytic B. anthracis strains isolated in Poland are closely related to the vaccine strain Steme 34F2 and may together constitute the same sensu stricto strain. No epidemiological link could be however traced between both the vaccine and the haemolytic strains.

20

New aminophosphonates - estimation of their potential pesticidal efficiency

72%

Dziamska A. , Bielecki K. , Kleszczynska H. , Sarapuk J.

|

2001

|

tom 12

219-226