Wyniki wyszukiwania - Biblioteka Nauki

1

Effect of amphotericin B on the biochemical markers in the haemolymph of honey bees

100%

Bajda M. , Los A. , Merska M.

Medycyna Weterynaryjna

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2014

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tom 70

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nr 12

EN

We examined the effect of amphotericin B (AmB) on the following enzymatic markers: aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), and on non-enzymatic markers: glucose, triglycerides, and proteins in the haemolymph of a model organism, Apis mellifera. AmB is an antifungal antibiotic. Despite its toxicity, it is used to treat disease conditions. The haemolymph of honey bees is considered as an analogue of human blood, and changes in marker values indicate pathological states, both in humans and honey bees. Three groups of caged bees were fed sugar syrup (the control group). The syrup was supplemented with AmB at concentrations of 0.25 mg/ml (AmB-25) and 0.50 mg/ml (AmB-50). The authors observed that the biochemical markers were age-related in the control group. Decreased values of the enzymatic markers in the AmB-treated groups confirm that AmB has a negative effect on the organism. The higher the dose of the antibiotic, the greater the increase in the concentration of the non-enzymatic markers. Our research shows that honey bees are an important model for studying the effects of AmB.

2

Antibacterial peptides of the moth Galleria mellonella

100%

Mak P. , Chmiel D. , Gacek G. J.

Acta Biochimica Polonica

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2001

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tom 48

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nr 4

1191-1195

EN

The work describes purification and biochemical characterization of two inducible antimicrobial peptides from the hemolymph of Galleria mellonella. The peptides were isolated by a sequence of reversed-phase chromatography steps from the hemolymph of larvae immunized with viable bacteria. The first peptide is a member of the cecropin family while the second one is rich in proline residues and has a unique sequence.

3

Two serine proteinase inhibitors from the larval hemolymph of Heliothis zea

100%

Polanowski A. , Wilusz T. , Blum M. S. , Travis J.

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nr 2

4

Serine proteinase inhibitors from insect hemolymph

100%

Polanowski A. , Wilusz T.

Acta Biochimica Polonica

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1996

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tom 43

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nr 3

EN

Insect hemolymph, like vertebrate serum, contains several different types of polypeptides that are able to inhibit the catalytic function of proteolytic enzymes, however studies on proteins possessing this capability have been limited to a relatively few species. A comparative examination of the inhibition of trypsin, chymo- trypsin, neutrophil elastase and cathepsin G and pancreatic elastase by the hemolymph of 14 insect species belonging to six orders showed great diversity in terms of both total proteinase inhibitory capacity and specificity. Most of the inhibitors examined fall into two groups: low molecular mass proteins (below 10 kDa) related to Kunitz type inhibitors, and proteins of about 45 kDa which belong to the serpin superfamily of serine proteinase inhibitors. This minireview describes the properties, characteristics and possible biological significance of selected inhibitors.

5

Histology of marbled crayfish Procambarus virginalis (Lyko, 2017): annotated atlas

86%

Marenkov O. , Lykholat T. , Kurchenko V. , Nesterenko O.

World News of Natural Sciences

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2018

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tom 21

141-153

EN

The educational publication contains microphotographs and description of histological preparations of the structure of marbled crayfish. The above materials may be used both for carrying out laboratory work on disciplines "Histology", "Cytology", "Cell Biology", "Special Practice", and for self-study of relevant educational topics. Designed for specialists in the field of hydrobiology and histology, students and graduate students of institutions of higher education who studying in the field of "091 Biology", "207 Water bioresources and aquaculture" and "162 Biotechnology and bioengineering". The publication contains the results of studies conducted by President’s of Ukraine grant for competitive projects Ф75/142 «The reproductive potential of invasive species of Dnieper region reservoirs and their impact on bioproductivity formation» (№ 0118U006319) of the State Fund for Fundamental Research.

6

The concentration of urea in hemolymph as a marker of health in Lissachatina fulica and Cornu aspersum edible snails – a preliminary study

86%

Ziętek J. , Guz L. , Wójcik A. , Winiarczyk S. , Adaszek Ł. , Ziętek J. , Guz L. , Wójcik A. , Winiarczyk S. , Adaszek Ł.

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7

Old hormones and new tricks: locust adipokinetic hormones revisited

86%

Goldsworthy G.

Pestycydy

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2005

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nr 4

33-41

8

Influence of low temperature on the synthesis of some Galleria mellonella proteins

86%

Kludkiewicz B. , Godlewski J. , Grzelak K. , Cymborowski B. , Lassota Z.

Acta Biochimica Polonica

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1996

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tom 43

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nr 4

EN

In developing Gallería mellonella larvae (reared at 30°C) three proteins of 74/ 76 and 81/82 kDa were identified. They represent a group of storage proteins (LHP proteins). In Galleria larvae, the development of which is arrested by low temperature (18°C)/ accumulation of the 74, 76 and 81/82 kDa proteins was detected in the hemolymph. The synthesis of 74 kDa and 76 kDa proteins started after 24 h, and that of about 80 kDa after 96 h following the transfer of larvae from 30°C to 18°C. 20-Hydroxyecdysone inhibited synthesis of the 74 and 76 kDa proteins in larvae exposed to low temperature. The arrest of development of Galleria larvae is associated with the synthesis and accumulation of storage proteins, and ecdysteroids are involved in these processes.

9

The activity of lisozyme in haemolymph of the bee colonies infected with Varroa jacobsoni after administration of levamizol

86%

Sokol R. , Romaniuk K. , Swiecicka-Grabowska G.

Wiadomości Parazytologiczne

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1998

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tom 44

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nr 3

10

Effect of Pseudomonas aeruginosa crude proteolytic fraction on antibacterial activity of Galleria mellonella haemolymph

86%

Andrejko M.

Folia Biologica

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2004

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tom 52

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nr 1-2

11

Cell-free immune response in juvenile hormone JH-III treated Galleria mellonella larvae

86%

Zdybicka-Barabas A.

Acta Poloniae Toxicologica

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1999

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tom 07

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nr 2

12

Developmental changes in the levels and redox potentials of main hemolymph thiols/disulfides in the Jamaican field cricket Gryllus assimilis

72%

Sadowska-Bartosz I. , Furmaniak P. , Bieszczad-Bedrejczuk E. , Bartosz G. , Głowacki R.

Acta Biochimica Polonica

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2017

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tom 64

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nr 3

503-506

EN

Main thiols and disulfides were determined in the hemolymph of the Jamaican field cricket Gryllus assimilis at various developmental stages. On the basis of these data, redox potentials of the glutathione, cysteine and homocysteine redox systems were calculated. The concentrations of all thiols studied decreased during development (at a stage of 6 molts) with respect to young crickets, and increased again in adult insects. Redox potentials of the glutathione and cysteine systems increased from values of -131.0±5.6 mV and -86.9±17.1 mV, respectively in young crickets to -58.0±3.6 mV and -36.1±4.2 mV, respectively, at the stage of 6 molts and decreased to values of -110.4±24.8 mV and -66.3±12.2 mV, respectively, in adult insects. Redox potentials of the glutathione and cysteine systems in the hemolymph of young and adult insects were similar to those reported for human plasma.

13

Influence of methoprene on the larval biochemistry in haemolymph and fat body of Ephestia cautella Walker (Lepidoptera: Pyralidae)

72%

Chandra A. , Tiwari S. K.

Journal of Biology and Earth Sciences

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2014

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tom 04

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nr 2

14

The mutual influence of proteins from Varroa destructor extracts and from honeybee haemolymph on their proteolytic activity – in vitro study

72%

Fraczek R. J. , Zoltowska K. , Lipinski Z. , Dmitryjuk M.

Acta Parasitologica

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2013

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tom 58

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nr 3

EN

The influence of extracts from Varroa destructor, a parasitic mite of the honeybee Apis mellifera, on the proteinase activity of worker bee haemolymph was analysed in vitro, along with the influence of bee haemolymph on the proteolytic activity of V. destructor extract. The study was conducted in three different environments: pH 7.5 (high activity of bee enzymes and very low activity of parasite enzymes), pH 5 (moderate activity of enzymes from both sources) and pH 3.5 (limited activity of bee proteinases and high activity of mite proteinases). Based on electrophoretic studies, the inhibition of the activity of bee haemolymph proteinases by V. destructor extracts was observed at each pH. The study at pH 7.5 with commercial inhibitors of the 4 main classes of proteinases (pepstatin A, ethylenediaminetetraacetic acid (EDTA), E-64 (trans-epoxysuccinyl-L-leucylamido-(4-guanidino)-butane), soybean trypsin inhibitor and Kunitz inhibitor) suggested that parasite extracts mainly inhibited serine proteinases and, to a lower degree, cysteine and aspartyl proteinases. At pH 3.5 and pH 5, a decrease of approximately 40% in parasite proteinase activity was also observed in the presence of bee haemolymph. The result points to the presence of aspartyl proteinase inhibitors in bee haemolymph, which may be an important defence element for bees during food intake by a mite. It was demonstrated that trypsin and trypsin inhibitors are active in the excretion/secretion products of V. destructor, the proteinases of which may assist the parasite in food suckling by preventing haemolymph coagulation, among other things.

15

The influence of hibernation on Dendrolimus pini haemocytes

72%

Kedra E. , Bogus M. I.

Pestycydy

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2005

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nr 4

139-142

16

Physiological effects of adipokinetic hormone, topically applied to the Mediterranean field cricket

72%

Lorenz M. W.

Pestycydy

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2005

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nr 3

109-116

17

Identification of specific interaction of juvenile hormone binding protein with isocitrate dehydrogenase

72%

Zalewska M. , Ozyhar A. , Kochman M.

Acta Biochimica Polonica

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2011

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tom 58

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nr 1

EN

Juvenile hormone (JH) is essential for multiple physiological processes: it controls larval development, metamorphosis and adult reproduction. In insect hemolymph more than 99 % of JH is bound to juvenile hormone binding protein (JHBP), which protects JH from degradation by nonspecific hydrolases and serves as a carrier to supply the hormone to the target tissues. In Galleria mellonella hemolymph, JHBP is found in a complex with lipid-binding high molecular weight proteins (HMWP) and this interaction is enhanced in the presence of JH. In this report, we present studies on the interaction of JHBP with low molecular weight proteins (LMWP) in the hemolymph. Using ligand blotting we found that JHBP interacts with a protein of about 44 kDa. To identify the protein that preferentially binds JHBP, a LMWP fraction was applied to a Sepharose-bound JHBP and, after washing, the column was eluted with free JHBP acting as a specific competitor or with carbonic anhydrase as a negative control. The eluted proteins were separated by SDS/PAGE and analyzed by mass spectrometry. Isocitrate dehydrogenase was identified as a component of the supramolecular complex of JHBP with hemolymph proteins.

18

The effect of a small conotoxin-like ctx gene from Autographa californica nuclear polyhedrosis virus (AcMNPV) on insect hemolymph melanization

72%

Cao Q. , Zhu S.-Y. , Wu Y. , Liu Y. , Zhu J. , Wang W.

Polish Journal of Microbiology

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2012

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tom 61

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nr 3

EN

The conotoxin-like (ctx) gene encodes a small cysteine-rich polypeptide in various baculoviruses. Previous research has demonstrated that the product of the ctx gene could be purified from insect cells infected by Autographa californica nuclear polyhedrosis virus (AcMNPV), but its function was unknown. In this paper, we compared the conserved cysteine motif structure (CX3GX2CX5CCX3CX6C) of the ctx gene in baculoviruses and generated recombinant Bombyx mori nuclear polyhedrosis virus (BmNPV) with the BmNPV bacmid system. !e recombinant BmNPV contained the ctx gene from AcMNPV or a fusion gene of ctx with eGFP, respectively. Fluorescence in CTX-eGFPpositive cells was mainly observed on the cell membrane. To gain insight into CTX function, two methods were used to elucidate the affect CTX had on hemolymph melanization in vivo and in vitro in insect larvae and pupae. The results indicated that CTX abrogates hemolymph melanization; however, the mechanisms require further evaluation.

19

Range extension of Dikerogammarus villosus (Sowinsky, 1894) in Poland (the Baltic Sea basin) and its ability to osmoregulate in different environmental salinities

72%

Dobrzycka-Krahel A. , Melzer M. , Majkowski W.

Oceanological and Hydrobiological Studies

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2015

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tom 44

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nr 3

20

Influence of proleg ligature on the protein level in haemolymph of Bombyx mori [L.] larvae

72%

Hyrsl P. , Marek M.

Biological Bulletin of Poznań

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1999

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tom 36

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nr 2