Wyniki wyszukiwania - Biblioteka Nauki

1

Analysis of peripheral blood white blood cell parameters in White stork (Ciconia ciconia L.) chicks varies by sex

100%

Grandtke M. , Kasprzak M. , Ciepliński M. , Burda E. , Durajski A. , Siekiera J. , Jerzak L.

Annales Universitatis Paedagogicae Cracoviensis Studia Naturae

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2018

EN

The aim our study was qualitative and quantitative analysis of white blood morphometric elements of peripheral blood (determining the quantity, blood cells dimension and several hematological values) in White Stork chicks. One of the aims was to indicate whether the sex relevantly influences the variety of the examined white blood indicators. White blood cells parameters of 53 White Stork chicks, with molecularly marked sex, was examined. Blood samples were collected in southern Poland (around Krapkowice town, near Opole city). Lymphocytes of White Storks (mean 37% for females and males) were identified as round cells with dark purple non-lobed, eccentrically positioned nucleus. Among the whole population we differentiated small lymphocytes: with diameters of 5.31 ± 0.65 μm in males and 5.57 ± 0.59 μm in females, and large lymphocytes with a diameter of 8.10 ± 0.66 μm and 8.28 ± 0.74 μm, respectively in females and males. Monocytes (mean 8% for female and males) were the largest leukocytes found in the blood film of White Stork, measuring 13.40 ± 0.97 μm for males and 13.09 ± 1.05 μm for females in diameter. The cytoplasm was abundant and it stained blue-gray, very often contained vacuoles. Heterophils (mean 42.7%) were the largest in granular leukocytes group. They were round and 11.14 ± 0.65 μm for males and 11.01 ± 0.48 μm for females in diameter. The nucleus of heterophils was lobed, usually with two or three lobes. The cytoplasm contained brick-red, elongated granules. Eosinophils (mean 9.44%) were round cells, with a diameter of 10.72 ± 0.49 μm and 10.97 ± 0.53 μm, respectively in males and females. The nucleus was lobed and mostly stained clear blue and contained red-orange, round or rod-shaped granules. Basophils (mean 1.84%) were round and contained dark blue granules, with average of 9.56 ± 0.78 and 9.13 ± 0.84 μm in diameter, for males and females. The nucleus was usually non-lobed. The H/L ratio was 4/4 for both sexes. No significant differences in levels and types of leukocytes between male and female juvenile storks have been observed.

2

The effects of rearing conditions on hematology and susceptibility of common carp to experimental manipulation stress

88%

Kondera E. , Witeska M.

Annals of Warsaw University of Life Sciences - SGGW. Animal Science

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2019

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tom 58[1]

3

Effect of milk replacer on calf rearing performance and hematological and biochemical blood indices

75%

Bogucki M. , Boguslawska-Tryk M. , Stanek M. , Kuropatwinska I. , Bujko J.

Acta Scientiarum Polonorum. Zootechnica

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2023

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tom 22

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nr 1

4

Biological variations of hematologic and biochemical parameters in cows during early lactation

75%

Kovačević V. , Cincović M. R. , Belić B. , Đoković R. , Lakić I. , Radinović M. , Potkonjak A. , Kovačević V. , Cincović M. R. , Belić B. , Đoković R. , Lakić I. , Radinović M. , Potkonjak A.

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5

Response of vitamins A, E, hematological and serum biochemical markers in Crucian carp (Carassius auratus gibelio) exposed to environmental Pb2+ and Cd2+

75%

Khan S. , Zhou P. , Liu X. , Li H. , Li J. , Rehman Z. u. , Ahmad I.

Acta Biochimica Polonica

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2015

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tom 62

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nr 3

581-587

EN

Toxicity of Pb2+ and Cd2+ is a widespread issue in the world; however, few studies have been conducted to understand their effect at environmentally realistic concentration in a mixture. In the present study, Crucian carp was exposed to Pb2+ (30 µg·l-1), Cd2+ (100 µg·l-1) and their mixture (30+100 µg·l-1) for 96 h and 21 d period to assess changes in the liver and muscle vitamin A and E content, and hematological and serum biochemical parameters. The results indicated significant decline in the level of antioxidant vitamins A, E and alterations in the hematological and serum biochemical indices. The toxicity revealed anemia, impairment of the liver and kidney with evident responses after 21 d exposure due to additive effect of Pb2+ and Cd2+ in mixture. Moreover, the differential response of vitamins A, E and blood parameters to low levels of waterborne Pb2+ and Cd2+ in freshwater fish can be used as biomarkers for monitoring contamination of aquatic environment.

6

Efficacy of β-hydroxy-β-methylbutyric acid (HMB) for growing rate and its influence for health indicators in blood test of young early-weaning goats

75%

Cebulska K. , Sobiech P. , Milewski S. , Ząbek K. , Cebulska K. , Sobiech P. , Milewski S. , Ząbek K.

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nr 1

7

Hematological alterations in common carp (Cyprinus carpio L.) exposed to herbicides: pendimethalin and ethofumesate tested separately and in mixture

75%

Bojarski B. , Ludwikowska A. , Kurek A. , Pawlak K. , Tombarkiewicz B. , Lutnicka H.

Folia Biologica

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2015

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tom 63

|

nr 3

8

Analysis of red blood cell parameters in dogs with various stages of degenerative mitral valve disease

75%

Kumiega E. , Michalek M. , Kasztura M. , Noszczyk-Nowak A.

Journal of Veterinary Research

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2020

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tom 64

|

nr 2

9

The 10th Congress of the European Hematology Association

75%

Wiktor-Jedrzejczak W.

|

2005

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tom 53

|

nr 4

10

Changes in the haematology of the Nile tilapia, Oreochromis niloticus Trewavas under the effect of crude oil

75%

Omoregie E.

Acta Hydrobiologica

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1998

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tom 40

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nr 4

11

Influence of zearalenone micotoxicosis on selected immunological, haematological and biochemical indexes of blood plasma in bitches

75%

Gajecka M. , Jakimiuk E. , Skorska-Wyszynska E. , Zielonka L. , Polak M. , Paluszewski A. , Rybarczyk L. , Gajecki M.

Polish Journal of Veterinary Sciences

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2004

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tom 07

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nr 3

EN

The immunological, haematological and biochemical analyses of blood plasma in bitches with 50 days lasting induced zearalenone micotoxicosis were carried out. It can be indirectly suggested that the inhibition of the humoral reaction of the organism, stimulation of detoxification effect in the liver and decreased cellular answer took place.

12

Fungal contamination of air in the department of haematology

63%

Nawrot U. , Usnarska-Zubkiewicz L. , Pajaczkowska M. , Mokracka-Latajka G. , Kuliczkowski K. , Nowicka J.

Polish Journal of Environmental Studies

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2010

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tom 19

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nr 5

EN

The aim of this study was to test the air in a single hospital department for fungal contamination. The department included three fully protected rooms with laminar air flow, comprising a bone marrow transplant unit (BMTU) and eleven naturally ventilated patient rooms of a haematology unit (HAEMU). Air samples were taken with an IDEAL air sampler (bioMerieux) on Sabouraud dextrose agar plates. The concentration of fungi in the air of the HEPA-filtered rooms of the BMTU ranged from 0-75 CFU/m3. Penicillium and Cladosporium were dominant among the fungal biota in the whole department. Of aspergilli, A. fumigatus was prevalent and seasonal increases in the frequencies of A. clavatus and A. niger isolation were observed. The detection of potentially pathogenic species of Aspergillus and Mucor in the BMTU and an increased concentration of Aspergillus in the HAEMU (up to 200 CFU/m3) instigated the introduction of additional preventive measure besides routine disinfection, namely an exchange of the HEPA filters in the BMTU and the installation of equipment based on multifunctional ion technology in the HAEMU. In a subsequent examination, a diminished number of fungi in the air was observed. During the study, 2 cases of proven and 3 of probable aspergillosis (according to EORT criteria) were noted. There was no link observed between the higher concentration of Aspergillus detected in the hospital air and the development of the infection. The authors conclude that hospital air examination can be helpful in indicating problems with hospital air facilities, enabling the introduction of procedures improving air quality and subsequently diminishing the risk of nosocomial mycoses.

13

Assessment of physiological status of felids as an indicator of their welfare in the wild

63%

Pavlova E. , Ivanov E. , Kirluk V. , Rozhnov V. , Naidenko S.

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tom 13

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nr 1

107-122

EN

One of the main approaches to monitoring the welfare of wild mammal populations in the wild is animal health estimation based on an analysis of their physiological parameters. To assess that for the natural felid populations, we analyzed adrenal activity level, white blood cells (WBC) counts and occurrence of seropositive reactions to different diseases. The first study was conducted in the Southwest Primorye, in natural habitats of Far Eastern leopard. Unevenly cold and snowy winters in 2010 and 2011 provided extreme natural conditions for overwintering mammals across the Russian Far East. Adrenocortical activity of leopards was significantly higher in 2011 (fecal cortisol level was 712.7 ± 92.4 ng g-1) than in 2010 (361.4 ± 80.5 ng g-1). We suggest that abundance and availability of weakened deer and carcasses could facilitate hunting for leopards and help them to avoid starvation. In the second study we used WBC counts as a health index for three felid species: Pallas cat (Daursky State Nature Biosphere Reserve, 2010), Far Eastern leopard (Southwestern Prymorie, 2011), Siberian tiger (Ussuriskii Reserve, 2010-2011). The ratio of neutrophils to lymphocytes (N:L) was used as an indicator of stress and/or disease state. Occurrence of seropositive reactions to 12 different pathogens was analyzed in all the animals after the hematological analysis. Pallas cats had the lowest N:L ratio (1.0 ± 0.1), significantly differing from leopards (42.8 ± 16.0) and tigers (24.5 ± 9.2). The N:L ratio correlated with the occurrence of seropositive reactions but not with the pathogen diversity. Pallas cats had the lowest occurrence of positive reactions to 4 pathogens: Toxoplasma gondii (12.5%), Mycoplasma sp. (12.5%), influenza A (7.1%), and feline leukemia virus (8.3%). Leopards had the highest N:L and occurrence of seropositive reactions only to 2 infections: T. gondii (16.6%) and Candida sp. (100%). On the contrary, tigers had positive responses to 6 pathogens, including canine distemper (7.7%), feline immunodeficiency virus (8.3%) and Aujeszky's disease (31%), which were not found in other cats. Values of N:L depended also on the time spent to get blood samples. In the big felid species effects of glucocorticoids on the WBC ratio (neutrophilia) were obtained in blood samples from anesthetized animals, taken more than 2 hours after capture. In Pallas cats blood samples were collected within 13 ± 2 min without anesthesia. Thus the high N:L ratio in big felids can be explained by several reasons: stress of capture, immunosuppression and a high diversity of detected infections. The present findings may have important implications for creation of effective conservation strategies of rare felid species in the wild.

14

Hematology of the freshwater fish, Notopterus notopterus in relation to Physico-chemical characteristics of the water

63%

Kulkarni R. S.

International Letters of Natural Sciences

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2015

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tom 40

EN

Hematological and some biochemical contents of the blood in relation to physico- chemical characteristics of the water have been studied in the fresh water fish, Notopterus notopterus. The results indicated that normal values have been found in comparison to the studies carried out in other fishes. Hence, the fish Notopterus notopterus is having favorable environmental temperature, pH and dissolved oxygen required for the fish to survive and regulate breeding activities.

15

Mikromacierze w hematologii : nowe kierunki diagnostyki hematologicznej

63%

Demkow U.

Laboratorium - Przegląd Ogólnopolski

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2012

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tom nr 5-6

36--38

PL

Mikromacierze znajdują coraz szersze zastosowanie w biologii, medycynie oraz w diagnostyce. Ważnym kierunkiem badawczo-rozwojowym jest zastosowanie mikromacierzy w diagnostyce hematologicznej, gdzie pozwalają one m.in. identyfikować geny istotne w patogenezie nowotworów hematoonkologicznych czy opracowywać nowe leki na podstawie oceny ekspresji genów w komórkach nowotworowych.

EN

Microarrays are becoming widely used in biology, medicine and diagnostics. An important direction of research and progress is the use of microarrays in the haematological diagnostics, where they allow to identify genes important in the pathogenesis of cancer. They are also useful for the development of new drugs based on the assessment of gene expression in cancer cells.

16

Age related changes and reference intervals of haematological variables in dairy calves

63%

Jezek J. , Nemec M. , Staric J. , Klinkon M.

Bulletin of the Veterinary Institute in Puławy

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2011

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tom 55

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nr 3

EN

The aim of the study was to investigate the physiological pattern of haematological variables and to acquire reference intervals for calves in different age periods. Sixty-five Holstein Friesian calves from two dairy farms were serially sampled from birth to the age of 24 weeks. Red blood cell count (RBC), haemoglobin concentration (Hb), mean corpuscular volume (MCV), haematocrit (PCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), number of platelets (PLT), and white blood cell count (WBC) were measured in blood samples. The health status of the calves was monitored. The age influenced significantly the values of all haematological variables (P<0.001). The effect of farm was significant for RBC (P=0.048) and MCHC (P<0.001), other variables did not differ significantly between farms. The temporal dynamics of RBC, Hb, and PCV values was harmonised. A progressive reduction in the values occurred over the first weeks of life; the lowest values were established at the age of 5 to 6 weeks. The reference intervals of haematological variables were calculated for three age groups of calves; 1-4 weeks, 5-8 weeks, and 12-24 weeks of age.

17

Wielometryczna cytometria przepływowa jako nowa technika w ręku diagnosty laboratoryjnego - cz. II

63%

Sędek Ł. , Mazur B.

Laboratorium - Przegląd Ogólnopolski

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2007

|

tom nr 4

40--46

PL

Próbka materiału poddawanego pomiarowi cytometrycznemu musi być odpowiednio przygotowana, w sposób zależny od jej rodzaju oraz od charakteru barwionych antygenów. W prawidłowo przeprowadzonym badaniu nie powinno brakować kontroli, zarówno negatywnej, jak i pozytywnej. Z analizy wyniku można uzyskać jakościowe i ilościowe informacje na temat populacji komórek i ekspresji antygenów. Parametry te są wysoce informatywne w wielu dziedzinach, takich jak hematologia, immunologia, diagnostyka medyczna, a także biofizyka, biochemia czy biologia komórki. Szybki rozwój cytometrii przepływowej w ostatnich latach i rozszerzenie wachlarza jej zastosowania są konsekwencją odkrywania nowych antygenów, barwników fluorescencyjnych i przeciwciał monoklonalnych oraz unowocześniania samych cytometrów.

EN

Flow cytometric assay requires appropriate material preparation, depending on the type of the sample and stained antigens. To determine the level of non-specific antibody binding an additional isotype control sample must be included. Flow cytometric assay produces qualitative data on cell populations and atigen expression, which is highly informative in many fields of science such as hematology, immunology, medical diagnostics, biophysics, biochemistry and cell biology. Rapid development of flow cytometry in recent time, and broadening of its appliacations are a consequence of continuous and successful chase of new antigens, fluorescent dyes, monoclonal antibodies, as well as implementation of modern technologies in fow cytometers

18

Effect of supplementation with copper in different chemical forms on selected physiological blood markers and content of minerals in selected tissues of turkeys

63%

Makarski B. , Gortat M.

Journal of Elementology

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2011

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tom 16

|

nr 4

EN

Scientific research has demonstrated that different compounds containing copper are capable of stimulating body weight gains, improving the health of animals and attaining high production performance without causing excessive accumulation of various chemical compounds in animal tissues. Besides, copper has been reported to exert a positive effect on the immune system, haematological blood markers and the microbiological balance in the gastrointestinal tract of turkeys. The experiment was conducted on 60 BUT-9 line turkeys. The birds were divided into 5 experimental groups. Each group included 12 turkeys kept in cages. The bird were reared for 19 weeks. The first group served as control. Group II received CuSO4 added to water in a dose of 10 mg Cu dm–3 H2O. Group III was com posed of birds administered a Cu chelate with lysine in a dose of 10 mg Cu dm–3 H2O. Group IV received CuSO4 in a dose of 20 mg Cu dm–3 H2O, whereas group V were given a Cu-lysine supplement in a dose of 20 mg Cu dm–3 H2O. The birds were fed commercial complete feed mixes. The copper preparations were added to drinking water since the 3rd week of rearing. The objective of this study has been to determine the effect of Cu supplementation in the organic and inorganic form and in two doses on the health of birds and on accumulation of minerals in their breast muscle and liver. Further analyses involved the determination of haematological and biochemical markers in blood as well as assays of selected minerals in the birds’ blood, breast muscle and liver. The analysis of the results indicated that the doses of copper improved the health of the turkeys, as verified by better haematological markers. Statistically significant difference appeared in the group receiving 20 mg Cu dm–3 H2O irrespective of the chemical form in which copper was administered. The study also shows that Cu supplementation has a significant effect on the metabolism of lipids and a non-significant impact on the content of mineral elements in the analyzed tissues. It was not until the 20 mg dose of Cu as a chelate had been applied that the accumulation of copper in the turkey’s liver was enhanced.

PL

Z badań naukowych wynika, że różne związki miedzi można zaliczyć do substancji stymulujących przyrosty masy ciała oraz poprawiających zdrowotność zwierząt, co sprzyja uzyskiwaniu lepszych efektów produkcyjnych, nie powodując nadmiernego akumulowania związków chemicznych w tkankach zwierząt. Ponadto miedź korzystnie wpływa na funkcjonowanie układu immunologicznego, poprawia wskaźniki hematologiczne krwi oraz umożliwia utrzymanie równowagi mikrobiologicznej przewodu pokarmowego indyków. Badania przeprowadzono na 60 indykach linii BUT-9 podzielonych na 5 grup doświadczalnych. Każda grupa liczyła po 12 indyków, które były utrzymane w klatkach. Odchów zwierząt trwał 19 tygodni. Pierwsza grupa stanowiła kontrolę. Grupa II otrzymywała dodatek CuSO4 do wody w ilości 10 mg Cu dm–3 H2O, grupa III – dodatek chelatu Cu z lizyną w dawce 10 mg Cu dm–3 H2O, grupa IV – dodatek CuSO4 w dawce 20 mg, grupa V – suplement Cu – lizynę w dawce 20 mg Cu dm–3 H2O. Paszę stanowiła typowa mieszanka pełnoporcjowa dostępna na rynku. Preparaty miedzi dodawano do wody pitnej od 3. tygodnia odchowu. Celem badań było określenie wpływu suplementacji Cu stosowanej w formie nieorganicznej i organicznej oraz w dwóch różnych dawkach na zdrowotność indyków oraz stopień akumulacji elementów mineralnych w mięśniu piersiowym i wątrobie. W badaniach oznaczono wskaźniki hematologiczne i biochemiczne krwi indyków oraz zawartość wybranych elementów mineralnych we krwi, mięśniu piersiowym i wątrobie ptaków. Wykazano, że zastosowane dawki miedzi spowodowały poprawę stanu zdrowia indyków, co wyrażało się korzystnymi zmianami wskaźników hematologicznych. Statystycznie istotne wartości odnotowano w grupie ptaków, które otrzymywały 20 mg Cu dm–3 H2O niezależnie od formy chemicznej. Z badań wynika, że suplementacja Cu ma znaczący wpływ na gospodarkę lipidową. Miedź miała nieznaczny wpływ na zawartość elementów mineralnych w tkankach. Jedynie dawka 20 mg Cu w formie chelatu spowodowała zwiększoną kumulację tego pierwiastka w wątrobach ptaków.

19

Zastosowanie NGS i GWAS w diagnostyce i prognozowaniu chorób hematologicznych

63%

Pierzyna-Świtała M. , Sędek Ł.

|

2017

|

tom nr 9-10

22--27

PL

W ostatnich latach obserwujemy gwałtowny rozwój diagnostyki laboratoryjnej w kierunku diagnostyki spersonalizowanej. W pracy przedstawiono nowoczesne metody molekularne, ich praktyczne zastosowanie we współczesnej diagnostyce chorób hematologicznych oraz perspektywy wykorzystania tych metod w przyszłości.

EN

In recent years we have observed a rapid development of laboratory diagnostics into personalized diagnostics. The article presents modern molecular methods and their practical application in the contemporary diagnostics of haematological diseases, as well as the prospects of their future clinical use.

20

Hematological and biochemical changes in naturally occurring equine piroplasmosis in donkeys (Equus asinus) of Northwest of Iran

63%

Afshar N. A. , Malekifard F. , Rezaei S. A. , Tavassoli M.

Acta Parasitologica

|

2020

|

tom 65

|

nr 4