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1 Medycyna Weterynaryjna

1 2009

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Mikrobiota grzybicza powlok zewnetrznych zwierzat wolno zyjacych

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Ziolkowska G. , Budziwojska M. , Nowakiewicz A. , Zieba P.

Medycyna Weterynaryjna

2009

tom 65

nr 10

719-722

The objective of the research was to assess the presence of mycotic microbiota on the integument of wild boars and roe-deer, as well as to isolate and identify each species. The research material comprised groin screening swabs collected from 13 wild boars and 56 roe-deer from the Lublin State Forests. The fungi were identified concurrently on the Sabouraud and MLNA medium at 25°C, 32°C and 37°C temperature for 14 days. Initial identification proceeded according to the conventional mycological procedures followed by the application of the commercial API Candida and API 20C Aux (bioMerieux) (Candida genus) tests and the phenotypic scheme developed by Guillot et al. (Malassezia genus). The present research has revealed that mycotic flora was recovered in all a total of 69 examined animals. The most frequently isolated fungi included Penicillium spp., Alternaria spp., Cladosporium spp. and Malassezia spp. and Rhodotorula spp. The species analysis of the isolated fungi has confirmed the presence of potential pathogens, such as Malassezia sympodialis, Aspergillus fumigatus and Candida non-albicans. The obtained results indicate that a population of free-living animals may constitute a critical link in the epidemiologic chain of mycotic infections.