Wyniki wyszukiwania - Biblioteka Nauki

1

Selenium supplementation to chronic kidney disease patients on hemodialysis does not induce the synthesis of plasma glutathione peroxidase

100%

Zachara B. A. , Gromadzinska J. , Zbrog Z. , Swiech R. , Wasowicz W. , Twardowska E. , Jablonska E. , Sobala W.

Acta Biochimica Polonica

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2009

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tom 56

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nr 1

183-187

EN

Background: Numerous authors have shown that selenium (Se) concentration and glutathione peroxidase (GSH-Px) activity in plasma of chronic kidney disease (CKD) patients are lower than in healthy subjects, but there are only few publications on the level of GSH-Px protein in those patients and no reports on the effect of Se supplementation to HD patients on the level of this enzyme. Subjects and Methods: Se concentration and GSH-Px protein level in plasma were measured in a group of 30 CKD patients on hemodialysis (HD) supplemented with 200 µg Se/day for 3 months, and 28 patients on HD administered with placebo. Se concentration was measured by graphite furnace atomic absorption spectrometry and plasma GSH-Px protein level by the sandwich ELISA method using polyclonal antibody specific for human plasma GSH-Px. Results: Se concentration in patients on placebo did not change throughout the 3-month study period, but increased significantly in Se supplemented group. Se supplementation to CKD patients on HD had no effect on the level of GSH-Px protein. Conclusions: The lack of GSH-Px protein in CKD patients on HD is not linked to Se deficiency since the level of this element increased after Se supplementation while enzyme protein level did not change. The damaged kidney of HD patients is unable to synthesize GSH-Px, even after induction with selenium.

2

Selenium, glutathione and glutathione peroxidases in blood of patients with chronic liver diseases.

100%

Czuczejko J. , Zachara B. A. , Staubach-Topczewska E. , Halota W. , Kędziora J.

Acta Biochimica Polonica

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2003

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tom 50

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nr 4

1147-1154

EN

Disturbances in the antioxidant system could play a role in pathogenesis of chronic liver disease. The aim of our study was to evaluate the levels/activities of antioxidants in blood of patients with chronic liver disease. We estimated selenium and glutathione concentrations and glutathione peroxidase activities in blood of 59 patients with chronic hepatitis B or C virus infection (group 1) and 64 patients with alcoholic, autoimmune or cryptogenic chronic liver disease (group 2). The results were compared with 50 healthy controls. Whole blood and plasma selenium and red cell glutathione concentrations were significantly lower in the patients compared with the controls. Red cell glutathione peroxidase activity was slightly reduced in both subgroups of group 1 and in group 2 with normal alanine aminotransferase values. Plasma glutathione peroxidase activity was slightly but significantly higher in patients with elevated aminotransferase values. The findings suggest that disturbances in antioxidant parameters in blood of patients with chronic liver disease may be the cause of the peroxidative damage of cells.

3

Glutathione and GSH-dependent enzymes in patients with liver cirrhosis and hepatocellular carcinoma

86%

Czeczot H. , Ścibior D. , Skrzycki M. , Podsiad M.

Acta Biochimica Polonica

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2006

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tom 53

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nr 1

237-242

EN

We investigated glutathione level, activities of selenium independent GSH peroxidase, selenium dependent GSH peroxidase, GSH S-transferase, GSH reductase and the rate of lipid peroxidation expressed as the level of malondialdehyde in liver tissues obtained from patients diagnosed with cirrhosis or hepatocellular carcinoma. GSH level was found to be lower in malignant tissues compared to adjacent normal tissues and it was higher in cancer than in cirrhotic tissue. Non-Se-GSH-Px activity was lower in cancer tissue compared with adjacent normal liver or cirrhotic tissue, while Se-GSH-Px activity in cancer was found to be similar to its activity in cirrhotic tissue and lower compared to control tissue. An increase in GST activity was observed in cirrhotic tissue compared with cancer tissue, whereas the GST activity in cancer was lower than in adjacent normal tissue. The activity of GSH-R was similar in cirrhotic and cancer tissues, but higher in cancer tissue compared to control liver tissue. An increased level of MDA was found in cancer tissue in comparison with control tissue, besides its level was higher in cancer tissue than in cirrhotic tissue. Our results show that the antioxidant system of cirrhosis and hepatocellular carcinoma is severely impaired. This is associated with changes of glutathione level and activities of GSH-dependent enzymes in liver tissue. GSH and enzymes cooperating with it are important factors in the process of liver diseases development.

4

Serum total antioxidant capacity and enzymatic defence of dogs with chronic heart failure and atrial fibrillation: a preliminary study

86%

Michalek M. , Tabis A. , Noszczyk-Nowak A.

Journal of Veterinary Research

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2020

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tom 64

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nr 3

5

Antioxidant supplementation to medium for in vitro embryo production in Felis catus

86%

Cocchia N. , Tafuri S. , Del Prete C. , Palumbo V. , Esposito L. , Avallone L. , Ciani F. , Cocchia N. , Tafuri S. , Del Prete C. , Palumbo V. , Esposito L. , Avallone L. , Ciani F.

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6

Red blood cell and plasma glutathione peroxidase activities and selenium concentration in patients with chronic kidney disease: A review

86%

Zachara B. A. , Gromadzińska J. , Wąsowicz W. , Zbróg Z.

Acta Biochimica Polonica

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2006

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tom 53

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nr 4

663-677

EN

The metabolism of oxygen in aerobic organisms leads to generation of reactive oxygen species (ROS). These entities are able to oxidize almost all classes of macromolecules, including proteins, lipids and nucleic acids. The physiological level of ROS is usually regulated by antioxidant defense mechanisms. There are at least three groups of antioxidant enzymes: superoxide dismutases, catalases and glutathione peroxidases (GSH-Pxs) which neutralize ROS. The trace elements (copper, zinc and selenium) bound to the active sites of the above listed enzymes play an important role in the antioxidant defense system. In mammals, a major function of selenium (Se) and Se-dependent GSH-Pxs is to protect cells from oxidative stress. Selenium concentrations and GSH-Px activities are altered in blood components of chronic kidney disease (CKD) patients. The Se level is frequently lower than in healthy subjects and the concentration very often decreases gradually with advancing stage of the disease. Studies on red cell GSH-Px activity in CKD patients reported its values significantly lower, significantly higher and lower or higher, but not significantly as compared with healthy subjects. On the other hand, all authors who studied plasma GSH-Px activity have shown significantly lower values than in healthy subjects. The degree of the reduction decreases gradually with the progression of the disease. High inverse correlations were seen between plasma GSH-Px activity and creatinine level. A gradual decrease in plasma GSH-Px activity in CKD patients is due to the fact that this enzyme is synthesized predominantly in the kidney and thus the impairment of this organ is the cause of the enzyme's lower activity. Se supplementation to CKD patients has a slightly positive effect in the incipient stage of the disease, but usually no effect was observed in end-stage CKD. Presently, kidney transplantation is the only treatment that may restore plasma Se level and GSH-Px activity in patients suffering from end-stage CKD. A few studies have shown that in kidney recipients, plasma Se concentration and GSH-Px activity are restored to normal values within a period of 2 weeks to 3 months following surgery and thus it can be acknowledged that Se supplementation to those patients has a positive effect on plasma GSH-Px activity.

7

Changes in GSH-antioxidant system induced by daunorubicin in human normal and diabetic fibroblasts.

86%

Zatorska A. , Maszewski J. , Jóźwiak Z.

Acta Biochimica Polonica

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2003

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tom 50

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nr 3

825-835

EN

We investigated the effect of daunorubicin on glutathione content and activity of GSH-related enzymes in cultured normal and diabetic human fibroblasts. Cells were incubated with 4 μM daunorubicin (DNR) for 2 h followed by culture in drug-free medium for up to 72 h. Treatment of diabetic cells with the drug caused a time-dependent depletion of intracellular GSH and a decrease of the GSH to total glutathione ratio. GSH depletion was accompanied by apoptotic changes in morphology of the nucleus. Analysis of GSH-related enzymes showed a significant increase of the activities of Se-dependent and Se-independent peroxidases and glutathione S-transferase. In contrast, glutathione reductase activity was reduced by 50%. Significant differences between normal and diabetic cells exposed to DNR were observed in the level of GST and Se-dependent glutathione peroxidase activities. These findings indicated that daunorubicin efficiently affects the GSH antioxidant defense system both in normal and diabetic fibroblasts leading to disturbances in glutathione content as well as in the activity of GSH-related enzymes.

8

Damage to erythrocytes caused by 2,3,7,8-tetrachlorodibenzo-p-dioxin [in vitro]

86%

Bukowska B.

Cellular and Molecular Biology Letters

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2004

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tom 09

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nr 2

EN

The effects of the exposure of human erythrocytes to different concentrations of 2,3,7,8-tetrachlorodibenzo-p-dioxin were studied. Particular attention was paid to lipid peroxidation, haemoglobin oxidation, and changes in the activity of catalase and glutathione peroxidase. Human erythrocytes at a 5% haematocrit were incubated with 2,3,7,8-TCDD at concentrations of 0.2 ppm to 1.6 ppm (ng-mg/ml erythrocytes) for 1 hour. The results obtained show that 2,3,7,8-TCDD induces the generation of lipid peroxides and the oxidation of Hb, and decreases the activity of catalase and glutathione peroxidase. This supports the thesis that TCDD causes oxidative stress in erythrocytes.

9

Influence of selenium compounds on glutathione peroxidase [GSH-Px] level in lamb blood

72%

Hadrys M. , Kinal S. , Antonowicz-Juchniewicz J. , Jedrychowska I.

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nr 1

10

Mimicking of glutathione peroxidase deficiency by exposition of JAR cells to increased level of synthetic hydroperoxide

72%

Hallmann A. , Milczarek R. , Szkatula M. , Wozniak M. , Spodnik J. H. , Klimek J.

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nr 4

304-308

EN

A short chain synthetic analogue of lipid hydroperoxides was used to overload glutathione peroxidase (GPx) in human choriocarcinoma cell line JAR cells. Cells exposed to 100 µM tBuOOH displayed a 40% reduction in ATP level and significantly increased in membrane permeability, visualised by the lactate dehydrogenase (LDH) release into the extracellular medium. The intracellular level of oxygen free radicals measured as an oxidation of the dichlorodihydro-fluorescein diacetate (H₂DCF-DA) significantly increased after 2 hours of cell exposition to 100 µM tBuOOH. Concomitantly MDA, 4-HNE level increased to 2 nmol/mg of cell protein after 2 hours. Mitochondria stained with MitoTracker Red CMXRos displayed a filamentous appearance in control cells but changed into granular less energised organelles after exposition to tBuOOH. Collectively, the above results indicate the importance of the contribution of oxidative stress in the development of pre-eclampsia.

11

The effects of soybean selenium proteinate on tissue selenium and meat quality traits in finishing pigs

72%

Svoboda M. , Fajt Z. , Banoch T. , Salakova A. , Drabek J.

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nr 1

EN

The purpose of the present study was to evaluate the effects of soybean selenium proteinate on Se tissue retention and meat quality in pigs. In group A (n=11) the mixtures were supplemented with soybean selenium proteinate, in group B (n=11) with sodium selenite and in group C (n=11) with Se-enriched yeast (0.3 mg Se per kg in all groups). The use of soybean selenium proteinate resulted in lower retention of Se in tissues (liver, heart, muscle) compared to Se-enriched yeast. Selenium concentrations in tissues achieved by soybean selenium proteinate and sodium selenite were comparable. No differences in serum Se, serum GSH-Px and meat quality traits were found among the groups.

12

Alterations in glutathione peroxidase and superoxide dismutase activities in plasma and saliva in relation to disease activity in patients with Crohn's disease

72%

Szczeklik K. , Krzysciak W. , Domagala-Rodacka R. , Mach P. , Darczuk D. , Cibor D. , Pytko-Polonczyk J. , Rodacki T. , Owczarek D.

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nr 5

13

Glutathione-dependent responses of plants to drought: a review

72%

Labudda M. , Azam F. M. S.

Acta Societatis Botanicorum Poloniae

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2014

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tom 83

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nr 1

EN

Water is a renewable resource. However, with the human population growth, economic development and improved living standards, the world’s supply of fresh water is steadily decreasing and consequently water resources for agricultural production are limited and diminishing. Water deficiency is a significant problem in agriculture and increasing efforts are currently being made to understand plant tolerance mechanisms and to develop new tools (especially molecular) that could underpin plant breeding and cultivation. However, the biochemical and molecular mechanisms of plant water deficit tolerance are not fully understood, and the data available is incomplete. Here, we review the significance of glutathione and its related enzymes in plant responses to drought. Firstly, the roles of reduced glutathione and reduced/ oxidized glutathione ratio, are discussed, followed by an extensive discussion of glutathione related enzymes, which play an important role in plant responses to drought. Special attention is given to the S-glutathionylation of proteins, which is involved in cell metabolism regulation and redox signaling in photosynthetic organisms subjected to abiotic stress. The review concludes with a brief overview of future perspectives for the involvement of glutathione and related enzymes in drought stress responses.

14

Selenium supplementation to chronic kidney disease patients on hemodialysis does not induce the synthesis of plasma glutathione peroxidase

72%

Zachara B. A. , Gromadzinska J. , Zbrog Z. , Swiech R. , Wasowicz W. , Twardowska E. , Jablonska E. , Sobala W.

Acta Biochimica Polonica

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2009

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tom 56

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nr 1

183-187

EN

Background: Numerous authors have shown that selenium (Se) concentration and glutathione peroxidase (GSH-Px) activity in plasma of chronic kidney disease (CKD) patients are lower than in healthy subjects, but there are only few publications on the level of GSH-Px protein in those patients and no reports on the effect of Se supplementation to HD patients on the level of this enzyme. Subjects and Methods: Se concentration and GSH-Px protein level in plasma were measured in a group of 30 CKD patients on hemodialysis (HD) supplemented with 200 μg Se/day for 3 months, and 28 patients on HD administered with placebo. Se concentration was measured by graphite furnace atomic absorption spectrometry and plasma GSH-Px protein level by the sandwich ELISA method using polyclonal antibody specific for human plasma GSH-Px. Results: Se concentration in patients on placebo did not change throughout the 3-month study period, but increased significantly in Se supplemented group. Se supplementation to CKD patients on HD had no effect on the level of GSH-Px protein. Conclusions: The lack of GSH-Px protein in CKD patients on HD is not linked to Se deficiency since the level of this element increased after Se supplementation while enzyme protein level did not change. The damaged kidney of HD patients is unable to synthesize GSH-Px, even after induction with selenium.

15

The protective effects of zinc in experimental gentamicin induced acute renal failure in rats

72%

Teslariu O. , Pasca A. S. , Mititelu-Tartau L. , Schiriac C. E. , Gales C. , Saftencu P. M. , Nechifor M.

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nr 5

16

Evaluation of oxidative stress parameters in dogs with brachycephalic obstructive airway syndrome before and after surgery

72%

Erjavec V. , Vovk T. , Nemec Svete A.

Journal of Veterinary Research

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2021

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tom 65

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nr 2

17

Isolation and computational characterization of glutathione peroxidase gene from an aquatic fern - Salvinia molesta

72%

Rahaman S. , Singh P. K. , Basu P. , Gupta S. , Basu M. , Ganguli S.

International Letters of Natural Sciences

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2016

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tom 51

EN

Pteridophytes and more specifically ferns represent a large but threatened group of plants which often serve as important environmental markers for pollution. Reports regarding stress responses in ferns are rare, apart from a few studies involving the ecological distribution and molecular marker studies. This work isolates a glutathione peroxidase enzyme from an aquatic fern widely distributed in fresh and polluted water bodies adjacent to sources of environmental polluted sources. Further computational analyses were performed to study the structure of the protein encoded by the open reading frame. Results indicate the presence of a large number of binding pockets which serve as important binding sites in the interactions with the cognate ligands.

18

Activities of glutathione peroxidase and catalase in organs from vanadium-treated rats

72%

Krosniak M. , Krzysciak W. , Kareta A. , Grybos R.

Acta Biologica Cracoviensia. Series Zoologia

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2009

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tom 51

39-48

19

Utilization of selenium compounds in nutrition of lambs

72%

Slupczynska M. , Kinal S. , Hadrys M. , Krol B.

Journal of Elementology

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2009

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tom 14

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nr 1

157-163

EN

The level of mineral utilization depends on many factors related to animals as well as to the chemical form of given nutrients. It has been experimentally demonstrated that animals utilize inorganic forms of minerals less efficiently than organic ones. Hence, an attempt has been made to evaluate the bioavailability of selenium bound in different compounds to lambs. Selenium supplementation in fodder mixtures was another aspect included in our tests. Thus, an experiment was conducted on 48 growing lambs, testing the level and chemical forms of selenium in fodder mixtures for animals. In group I (the control) lambs received fodder mixture without any selenium supplement. In the experimental groups, selenium was supplemented as sodium selenite in the amount 0.2 mg Se kg-1 d.m. feeds (II), or selenium enriched yeast (Se-yeast) in the amounts of 0.1 and 0.2 mg Se kg-1 d.m. of feed groups III and IV, respectively. At the end of the experiment, when animals had reached 35 kilos of weight, blood samples were taken. The activity of glutathione peroxidase was estimated in heparinized blood samples. Eight lambs were chosen from each group and killed to collect samples of soft tissues (liver, kidney, brain, muscle). The content of selenium was determined in the tissue samples. Supplementation of feeds for lambs with selenium, both inorganic (sodium selenite) and organic (Se-yeast), increased the content of the element in soft tissues of animals. The highest level of the element was found in the liver and kidneys: 4.65 and 4.90 and 2.10 and 2.30 mg kg-1 fresh tissue, of the lambs receiving sodium selenine and Se-yeast in the amount 0.2 mg Se kg-1 D.M. of feed (groups II and IV), respectively. Selenium compounds added to feeds given to lambs significantly (P£0.01) increased activity of GSH-Px in blood, especially in the case of animals which received yeast enriched with selenium. In blood of the lambs which received feeds with Se-yeast in the amount 0.2 mg Se kg-1 d.m. of feed (group IV), the activity of glutathione peroxidase was by 129.71 U gHb-1 higher, and of the animals which received Se-yeast in the amount 0.1 mg kg-1 d.m. or sodium selenite, the activity of the enzyme was higher by 86.33 and 86.35 U gHb-1, respectively, than the activity of this enzyme in blood of animals from the control group. Supplementation of lambs’ rations with Se in the form of selenite or yeast enriched with selenium forms increased the content of Se in soft tissues and gluthatione peroxidase activity in comparison with animals which did not receive additional doses of this nutrient in fodder mixtures. The availability of Se was more profoundly affected by the amount of the element added rather than its form.

PL

Stopień wykorzystania składników mineralnych z paszy zależy od wielu czynników związanych zarówno ze zwierzęciem, jak i z formą chemiczną stosowanych związków. W badaniach wskazano, że związki nieorganiczne są najczęściej gorzej wykorzystywane niż ich organiczne odpowiedniki. Dlatego podjęto próbę oceny przyswajalności przez jagnięta różnych związków selenu, uwzględniając także poziom dodatku tego pierwiastka w mieszankach. W badaniach na 48 rosnących jagniętach czynnikiem różnicującym poszczególne grupy były różne poziomy i formy połączeń chemicznych selenu w mieszankach treściwych. W grupie I (kontrolnej) jagnięta otrzymywały mieszankę bez dodatku selenu. W grupach (II) doświadczalnych mieszanki uzupełniano seleninem sodu w ilości 0.2 mg Se kg-1 s.m. paszy, a w grupach III i IV – drożdżami wzbogaconymi w selen (drożdże-Se), odpowiednio w ilości: 0.1 i 0.2 mg Se kg-1 s.m. paszy. Na końcu doświadczenia, po osiągnięciu przez zwierzęta masy ciała ok. 35 kg, pobrano od nich krew. Następnie w pełnej heparynizowanej krwi oznaczono aktywność peroksydazy glutationowej (GSH-Px). Z każdej grupy wybrano losowo po 8 jagniąt, zwierzęta ubito i pobrano tkanki miękkie (wątroba, nerki, mózg, mięsień przywodziciel uda). W zliofilizowanym materiale biologicznym oznaczono zawartość selenu. Podane jagniętom w mieszankach treściwych zarówno nieorganiczne (selenin sodu), jak i organiczne (drożdże-Se) formy połączeń selenu zwiększyły zawartość tego pierwiastka w tkankach miękkich jagniąt. Najwięcej selenu znajdowało się w wątrobie i nerkach, odpowiednio 4.65 i 4.90 oraz 2.10 i 2.30 mg kg-1 świeżej tkanki jagniąt otrzymujących w paszy selenin sodu i drożdże–Se w ilości 0.2 mg Se kg-1 s.m. paszy (gr. II i IV). Stosowane w paszy jagniąt związki selenu wyraźnie (P≤0.01) zwiększyły aktywności GSH-Px we krwi owiec, zwłaszcza u zwierząt, które w mieszance otrzymywały drożdże wzbogacone w selen. U owiec otrzymujących w paszy drożdże-Se w ilości 0.2 mg Se kg-1 s.m. paszy (grupa IV) wykazano wyższą o 129.71 U gHb-1 aktywność peroksydazy glutationowej, a u zwierząt, którym w paszy podawano drożdże-Se w ilości 0.1 mg Se kg-1 s.m. paszy lub selenin sodu, aktywność tego enzymu we krwi była wyższa odpowiednio o 86.33 i 86.35 U gHb-1 w porównaniu z grupą kontrolną. Zastosowanie w dawkach dla jagniąt dodatku Se, zarówno w formie seleninu, jak i wzbogacanych drożdży, spowodowało wzrost jego zawartości w tkankach miękkich oraz wzrost aktywności peroksydazy glutationowej w porównaniu ze zwierzętami z grupy kontrolnej. Większy wpływ na dostępność Se miał jego poziom w dawce niż stosowana forma.

20

Characterization and analyses of hydrophobic clusters, acetylation and myristoylation sites in plant glutathione peroxidase sequences

72%

Ganguli S. , Polley A. , Datta A.

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tom 01

EN

Incomplete reduction of oxygen molecules is the primary source for the formation of reactive oxygen species (ROS) during cytosolic metabolism or mitochondrial respiration. These phenomenons may be as a result of biotic or abiotic stress. Exposure to exogenous stimuli such as radiation might be an alternative pathway of ROS production. Thus plants require counter defense strategies to combat the increase of this toxic molecular build up in its cell cytoplasm. As a result they have devised an army of free radical scavenging enzymes which enable them to dissipate the oxidative stress imposed by the accumulation of these toxic moieties. Glutathione Peroxidase forms an important part of this arms race along with several catalases and organelle specific enzymes such as superoxide dismutase. Plant glutathione peroxidases(GPXs) have been studied exclusively for their evolutionary lineages since they represent a hybrid class of molecules in context of the presence and absence of selenocysteine at their catalytic centres, the former situation predominant in non vascular plant groups while the later a predominant feature of vascular plants. This analysis focuses on three important aspects of protein structure analyses – hydrophobic cluster analyses for identification of homologues, and acetylation and myristoylation sites which provide us with information regarding the post translational modifications of a particular protein group. Specific patterns of clusters along with acetylation and myristoylation site frequencies were obtained which indicate that GPXs of non vascular plant members possess less chances of getting myristoylated while acetylation was predominant in most land plant lineages but absent in aquatic members.