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Znaczenie plazmidowego zgrupowania genow spv u Salmonella enterica subsp.enterica

100%

Binek M. , Madajczak G.

nr 09

911-914

Znaczenie plazmidowego zgrupowania genow spv w chorobotworczosci paleczek Salmonella enteritidis dla kur. I. Wystepowanie zgrupowania genow spv w duzych plazmidach zjadliwosci paleczek Salmonella enteritidis

67%

Madajczak G. , Binek M.

nr 2

163-174

Zakażenia pałeczkami Salmonella są powszechne wśród ludzi i zwierząt, występują na całym świecie, zarówno w krajach wysoko rozwiniętych, cechujących się znacznym poziomem higieny, jak i w krajach ubogich, z niedostateczna opieką zdrowotną. Z tych przyczyn salmoneloza jest nadal poważnym zagrożeniem dla zdrowia człowieka. Problem ten może okresowo urastać nawet do rangi politycznej, jak np. w przypadku wybuchu epidemii.

Many Salmonella Enteritidis virulence factors are encoded by genes localized on plasmids, especially large virulence plasmid, in highly conserved fragment, they create spv plasmid gene group. The aims of realized researches were spv genes occurrence evaluation and composition analysis among Salmonella Enteritidis strains caused infection in chickens. Researches were realized on 107 isolates, where in every cases large virulence plasmid 59 kbp size were detected. Specific nucleotides sequences of spv genes (spvRABCD) were detected in 47,7% of isolates. In the rest of examinated bacterias spv genes occurred variably. Most often extreme genes of spv group, like spvR and spvD were absent, what could indicate that factors encoded by them are not most important for Salmonella Enteritidis live and their expressed virulence.

Ocena występowania plazmidowych genów zjadliwości spv oraz antybiotykooporność szczepów Salmonella izolowanych z mleka

67%

Wiszniewska-Laszczych A. , Zdrodowska B. , Wysok B. , Szteyn J. , Liedtke K. , Gomolka-Pawlicka M. , Wojtacka J.

Medycyna Weterynaryjna

2018

tom 74

nr 01

The aim of the study was to measure the frequency of occurrence of Salmonella spp. in raw milk, to identify their serotype, as well as to determine their antibiotic resistance and the presence of Salmonella plasmid virulence (spv) genes. Out of 300 bulk tank milk samples, 5.3% were contaminated with Salmonella spp. All strains isolated belonged to the serovar S enteritidis, as confirmed by serotyping and molecular methods. The presence of spv genes was determined by PCR. Spv genes were present, in different patterns, in all strains tested. SpvA gene was present in all isolates (100%), spvB in 56.25%, spvC in 62.5%, spvD in 75%, and spvR in 56.25%. Antibiotic resistance was evaluated according to the NCCLS recommendations. All isolates were sensitive to ciprofloxacin (CIP), gentamicin (GE), and chloramphenicol (CH). Thirteen strains were resistant to ampicillin (AMP), 8 to erythromycin (E), 1 to doxycycline (DO), and 1 to tetracycline (TE). Different frequency of occurrence of the spv genes in Salmonella strains isolated from raw milk demonstrates their high adaptability. As many as 87.5% of isolates showed resistance to at least one of the antibiotics tested.