Wyniki wyszukiwania - Biblioteka Nauki

1

One-step fabrication of gentamicin nanoparticles embedded in polymeric biomaterials surface: sonochemical approach

100%

Gołda-Cępa M. , Chytrosz P. , Kotarba A.

Engineering of Biomaterials

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2017

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tom Vol. 20, no. 143 spec. iss.

20

2

Halloysite nanotubes as potential carrier for gentamicin

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Stodolak-Zych E. , Skóra K. , Rapacz-Kmita A. , Mikołajczyk M. , Gajek M. , Bućko M. M.

Engineering of Biomaterials

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2018

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tom Vol. 21, no. 148

93

3

Fotokatalityczna degradacja gentamycyny w roztworach wodnych

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Makowski A. , Baran W. , Adamek E. , Nocoń W.

Proceedings of ECOpole

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2011

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tom Vol. 5, No. 2

561--566

PL

Metody zawansowanego utleniania polegające na powstawaniu wysokoreaktywnych rodników hydroksylowych są stosowane do usuwania substancji leczniczych z roztworów wodnych. Mogą być stosowane do degradacji antybiotyków i ich metabolitów do związków przejściowych, które następnie ulegają łatwiejszej biodegradacji. Same antybiotyki należą do tzw. związków refrakcyjnych, czyli nieulegających łatwo biodegradacji. Roztwory gentamycyny naświetlano promieniowaniem UV λ = 366 nm w obecności fotokatalizatora P 25 Degussa. Zawartość gentamycyny badano metodą HPLC. Stałe szybkości fotokatalitycznego rozkładu wyznaczano z zależności logC/Co od czasu trwania procesu. Jony żelaza i kobaltu przyspieszają proces fotokatalitycznego rozpadu gentamycyny, natomiast jony miedzi, wapnia i magnezu spowalniają ten proces. Na powierzchni P 25 następuje proces adsorpcji gentamycyny; proces ten nasila się w obecności jonów metali, zwłaszcza jonów żelaza. Najszybsza mineralizacja gentamycyny następuje również w obecności jonów żelaza. Jony żelaza, wapnia i magnezu tworzą kompleksy z gentamycyną o stosunku molowym 1:1, rozpadające się w trakcie naświetlania. Jony miedzi osadzają się na powierzchni fotokatalizatora podczas procesu fotokatalitycznego.

EN

Drugs and their metabolites belong to the group of compounds which can be micropollutants present in surface waters, sewage and soil. The widespread use of pharmaceuticals for the human health care results in their undesirable accumulation in the environment. The most hazardous groups of drugs are antibiotics because they are hardly biodegradable and can cause the formation of a drug resistance in numerous bacteria strains. The aim of the work was to investigate of the photocatalytic degradation of aminoglycoside antibiotic, namely gentamicin, the influence of metal ions (Fe2+, Co2+, Cu2+, Mg2+ and Ca2+). It was stated that the iron and cobalt ions accelerated the process of the photocatalytic degradation of gentamicin, while copper, calcium and magnesium slowed down this process. It was observed the adsorption of gentamicin on the photocatalyst surface. The fastest mineralization of organic substances was obtained in the presence of iron ions. It was found that the iron ions formed stable complexes with gentamicin (in the molar ratio 1:1).

4

A new simple liquid chromatographic assay for gentamicin in presence of methylparaben and propylparaben

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Ivković B. , Milutinović I. , Čudina O. , Marković B.

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tom Vol. 35, no. 1

81--87

EN

Gentamicin sulfate is a potent broad spectrum aminoglycoside antibiotic which is used against Gram-positive and Gram-negative bacteria. A simple, isocratic HPLC method for separation, identification and determination of gentamicin and parabens (methylparaben and propylparaben) was developed and validated. To our knowledge there is no report about simultaneous determination of those three analytes in pharmaceutical products. The optimum chromatographic conditions were achieved on CN column with a mobile phase consisting of 0.15% triethylamine in 10 mM KH₂PO₄ aqueous solution (final pH 3.0 adjusted with H₃PO₄) and methanol in the ratio 70:30 (v/v), providing selective quantification of analytes within 5 min. The method was successfully validated according to ICH guidelines acceptance criteria in terms of selectivity, linearity, accuracy, precision and robustness. The linearity of the method was proved in defined concentrations ranges for gentamicin (0.32–1.04 mg mL⁻¹), methylparaben (0.0072–0.0234 mg mL⁻¹) and propylparaben (0.0008–0.0026 mg mL⁻¹). Relative standard deviations calculated for all analytes in precision testing were <2% (analysis repeatability) and <3% (intermediate precision). Recovery values were between 98.87% and 101.67%. Chromatographic parameters are not significantly influenced by small variations of column temperature, pH and molarity of KH₂PO₄. Finally, the method was successfully applied for quantitative determination of gentamicin and parabens in commercially available solution for injection. Proposed HPLC method is found to be promising in terms of simplicity, analysis times and non-use of derivatization and ion-pair agents.

5

Wpływ modyfikacji białkowej na właściwości hydroksyapatytu jako nośnika gentamycyny

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Zalewska J. , Ginalska G. , Ślósarczyk A. , Godlewski P.

Inżynieria Biomateriałów

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2006

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tom R. 9, nr 58-60

214-216

6

Optymalizacja warunków wiązania gentamycyny na różnych typach protez naczyniowych

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Osińska-Jaroszuk M. , Ginalska G.

Inżynieria Biomateriałów

|

2006

|

tom R. 9, nr 58-60

170-172

7

Analysis of ciprofloxacin and gentamicin diffusion in Proteus mirabilis O18 biofilm by laser interferometry method

88%

Arabski M. , Wąsik S. , Zych M. , Łakomiec W. , Kaca W.

Acta Biochimica Polonica

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2013

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tom 60

|

nr 4

707-711

EN

Laser interferometry is a measurement technique used in physical sciences, with a potential for new applications in microbiology. Our previously studies, focused on the quantitative analysis of antibiotics diffusion through membranes or their releasing from gel structure, indicate that this method might be useful in analysis of substances diffusion across the bacterial biofilms. As antibiotic - biofilm interaction model, we tested above method for determination of ciprofloxacin or gentamicin diffusion through Proteus mirabilis O18 biofilm. Laser interferometry analysis of antibiotics diffusion showed that the amount of ciprofloxacin transported through mature biofilm is 1.9 times higher than gentamicin. It was correlated with lower level of gentamicin in compare to the level of ciprofloxacin in biofilm, which amounts were predicted in biofilm during diffusion process by laser interferometry method. We suggest that the analysis of antibiotic diffusion in biofilm might by helpful in evaluation of effectiveness of antibacterial agents.

8

CHITOSAN/POLY(VINYL ALCOHOL) HYDROGELS AS CONTROLLED DRUG DELIVERY SYSTEMS

88%

Kocemba A. , Mucha M.

Progress on Chemistry and Application of Chitin and its Derivatives

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2017

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tom 22

97-105

EN

In order to achieve hydrogel and drug release profiles, a comprehensive knowledge of the types, properties and syntheses of hydrogel polymer networks are needed. For this reason, a natural biopolymer hydrogel based on chitosan was described. Chitosan has many advantages, which meet the requirements necessary for the preparation of medical materials; for example, wound dressings. This article focused on the biomedical use of a chitosan hydrogel: chitosan–poly(vinyl alcohol) (PVA). The method of preparation of hydrogels containing a drug as an active wound dressing was described. To obtain a hydrogel dressing to be applied in patients with burns or difficult curative wounds, gentamicin (an aminoglycoside antibiotic) was used as a medicament. The effect of the PVA concentration in hydrogels on the release rate of the antibiotic was examined. For this, the crosslinking agent of the hydrogel, glutaraldehyde, was used. The release process of gentamicin was described by using an equation of first order kinetics.

9

Comparison of in vitro antibacterial activity of meropenem and gentamicin encapsulated in liposomes

88%

Drulis-Kawa Z. , Gubernator J. , Dorotkiewicz-Jach A. , Doroszkiewicz W. , Kozubek A.

Cellular and Molecular Biology Letters

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2005

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tom 10

|

nr Suppl.

10

Glucose and glyoxal modify the diffusion of gentamicin through the peritoneal membrane in vitro

88%

Szary B. , Czyzewska K. , Grzelak T.

Cellular and Molecular Biology Letters

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2002

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tom 07

|

nr Suppl.

11

A new achievement in obtaining durable antibacterial protection for vascular prostheses

75%

Ginalska G. , Osińska M. , Urbaniak-Sypniewska T. , Uryniak A. , Łobarzewski J.

Biocybernetics and Biomedical Engineering

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2004

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tom Vol. 24, no. 1

17-24

EN

Gentamicin covalently bound to bead shaped silica supports or Tricogel (vascular prosthesis) demonstrates stability for even up to 30 days. A comparison was made of the results of gentamicin immobilization to silica supports or Tricogel pieces activated in two modes. Gentamicin retention (during a 30-day experiment) on the Tricogel was shown to be higher than that on silica supports. The release of gentamicin from four matrices was observed only during the first 4 days of the 30-day experiments. The biological activity of gentamicin against "Escherichia coli", "Pseudomonas aeruginosa" and "Staphylococcus aureus" was also checked. In these experiments we used samples of Tricogel irradiated in UV (control), soaked in gentamicin solution or containing gentamicin covalently bound to this matrix. The covalently bound gentamicin was shown to inhibit completely the growth of all the three bacterial strains during the experiment.

12

Indirect spectrophotometric determination of gentamicin and vancomycin antibiotics based on their oxidation by potassium permanganate

75%

El-Didamony A. , Amin A. , Ghoneim A. , Telebany A.

Open Chemistry

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2006

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tom 4

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nr 4

708-722

EN

Four simple, accurate, sensitive and economical procedures (A–D) for the estimation of gentamicin sulphate and vancomycin hydrochloride, both in pure form and in pharmaceutical formulations have been developed. The methods are based on the oxidation of the studied drugs by a known excess of potassium permanganate in sulphuric acid medium and subsequent determination of unreacted oxidant by reacting it with amaranth dye (method A), acid orange II (method B), indigocarmine (method C) and methylene blue (method D), in the same acid medium at a suitable λmax=521, 485, 610 and 664 nm, respectively. The reacted oxidant corresponds to the drug content. Regression analysis of Beer-Lambert plots showed good correlations in the concentration ranges 4–8, 3–8, 4–9 and 5–9 µg ml−1 with gentamicin and 4–8, 1.5–4, 1.5–4 and 3.5–5.5 µg ml−1 with vancomycin for methods A, B, C, and D, respectively. The molar absorptivity, sandell sensitivity, detection and quantification limits were calculated. The stoichiometric ratios for the cited drugs were studied. The optimum reaction conditions and other analytical parameters were evaluated. The influence of the substance commonly employed as excipients with these drugs were studied. The proposed methods were applied to the determination of these drugs in pharmaceutical formulations. The results have demonstrated that the methods are equally accurate and reproducible as the official methods.

13

The use of a hydrocolide dressing on a chronic wound - case study

75%

Wilczyńska A. , Wójcik M. , Przekora A. , Ziętek J. , Adaszek Ł.

Engineering of Biomaterials

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2021

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tom Vol. 24, no. 163

58

14

Microscopic-electron examinations of the influence of the selected antibiotics on the secretory cells of the mammary gland

75%

Cegielski M.

Folia Histochemica et Cytobiologica

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1986

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tom 24

|

nr 4

15

Evaluation of the developmental toxicity of gentamicin using postimplantation rat whole embryo culture

75%

Biernacki B. , Wlodarczyk B. , Minta M.

Bulletin of the Veterinary Institute in Puławy

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2001

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tom 45

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nr 2

EN

The embryotoxicity of gentamicin was evaluated with rat whole embryo culture (WEC) assay. Rat embryos displaying 1-3 somites were explanted on gestation day (GD) 9.5 and cultured for 48 h in rat serum. Gentamicin sulfate was added to the culture medium at a concentration of 1, 10 and 100 μg/ml. At the end of the cultivation five endpoints were evaluated for each viable embryo: yolk-sac diameter, crown-rump length, head length, somites number and morphological score. Gentamicin at the concentration of 100 μg/ml was embryotoxic and caused growth retardation in rat embryos. At the concentration of 10 μg/ml about 45% of embryos showed haematomas. The lowest dose of antibiotic (1 μg/ml) did not interfere with embryonic development in vitro.

16

Study on the role of urine gamma-glutamyl transpeptidase activity during investigation of nephrotoxicity

75%

Kocaoglu S. , Berkan T. , Karan A. , Basdemir G.

Archivum Immunologiae et Therapiae Experimentalis

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1994

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tom 42

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nr 2

17

Distribution and amount of cathepsin B in gentamicin-induced acute kidney injury in rats

63%

Svara T. , Pogacnik M. , Juntes P.

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tom 13

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nr 1

75-82

EN

The aim of our study was to investigate how the distribution and amount of cathepsin B change during acute kidney injury. The research was done on a rat model of acute kidney injury that was induced by nephrotoxic antibiotic gentamicin. Gentamicin was injected at a dose of 40 mg/kg body weight (the first treated group) and 80 mg/kg body weight (the second treated group) for 14 days. Control groups received injections of physiological saline only. One day after the last injection, animals were euthanized, dissected and kidney samples were taken and fixed in 10% buffered formalin. Tissue sections were stained with haematoxylin and eosin, periodic Acid Schiff (PAS) and Oil-red-O. Immunohistochemistry was used for the demonstration of cathepsin B. Vacuolar degeneration of the proximal convoluted tubules was the most prominent pathologic lesion found in the first treated group, while necrosis prevailed in the second treated group in the same localisation. In both treated groups significantly weaker immunohistochemical reaction for cathepsin B was noticed in the proximal convoluted tubules in comparison to the control groups (P < 0.05). The decrease of positive reaction was the largest in the proximal convoluted tubules of the outer renal cortex. Stronger positive reaction for cathepsin B, although not statistically significant, was found in the proximal straight tubules (P > 0.05), as well. However, more numerous cathepsin B-positive large granules appeared in the proximal straight tubules of the second treated group then in the second control group (P < 0.05). We can conclude that the amount of cathepsin B in the affected proximal convoluted tubules significantly decreases along the increased severity of the histopathological lesions of the proximal convoluted tubuls, the amount of enzyme in the well preserved proximal straight tubules increases and more cathepsin B-positive large granules appear in the cytoplasm.

18

The protective effects of zinc in experimental gentamicin induced acute renal failure in rats

63%

Teslariu O. , Pasca A. S. , Mititelu-Tartau L. , Schiriac C. E. , Gales C. , Saftencu P. M. , Nechifor M.

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nr 5

19

Polymyxin B, gentamycin and neomycin inhibit phagocytic activity of Tetrahymena pyriformis

63%

Krawczynska W.

Acta Protozoologica

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1990

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tom 29

|

nr 3

20

Uwalnianie gentamycyny z nośników polimerowych

63%

Morawska-Chochół A. , Chłopek J.

Engineering of Biomaterials

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2012

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tom Vol. 15, no. 114

21--27

PL

Siarczan gentamycyny jest skutecznym antybiotykiem stosowanym w leczeniu głębokich infekcji kości. Jednak jego miejscowe dostarczanie za pomocą implantowanych nośników leków wymaga znajomości mechanizmu i kinetyki jego uwalniania, co powinno pozwolić w sposób kontrolowany na jego dozowanie w zależności od potrzeb i miejsca implantacji. Celem pracy była ocena wpływu lokalizacji gentamycyny w materiale nośnika (matryca/modyfikator) oraz typu modyfikatora zawierającego lek (kapsułki alginianowe/włókna PLA) na szybkość uwalniania gentamycyny. Jako matrycę w przypadku wszystkich badanych materiałów zastosowano polilaktyd (PLA). Szybkość uwalniania leku określono metodą spektrofotometryczną UV-vis. Wyniki te poparto pomiarami pH i przewodnictwa płynów inkubacyjnych. Dodatkowo wykonano obserwacje mikroskopowe wraz z mikroanalizą rentgenowską (SEM+EDS). Wykonano również badania spektroskopowe w podczerwieni (FTIR). Uwalnianie gentamycyny z polimerowego nośnika zachodzi najintensywniej w czasie pierwszego tygodnia inkubacji pomimo braku degradacji osnowy w tym okresie. Większej szybkości uwalniania oraz większej ilości uwalnianego leku sprzyja obecność granic międzyfazowych. Oznacza to, że umieszczenie gentamycyny w fazie modyfikującej osnowę (włókna, kapsułki) ułatwia uwalnianie leku z materiału. Uwalnianie antybiotyku z kapsułek z alginianu wapnia przebiega szybciej niż z włókien PLA, co wiąże się prawdopodobnie z ich znacznym pęcznieniem w środowisku wodnym, co tym samym ułatwia uwalnianie leku.

EN

Gentamicin sulfate is an effective antibiotic used in the treatment of deep bone infections. However, its local provision by means of implanted medicine carriers requires the knowledge of the mechanism and kinetics of its release, which should allow for its dosage in a controlled manner, depending on the need and the implant's location. The aim of the work was an evaluation of the effect of the gentamicin's location in the carrier material (matrix/modifier) and the type of the medicine-containing modifier (alginate capsules/PLA fibres) on the rate of the gentamicin release. In the case of the examined materials, polylactide (PLA) was applied as the matrix. The rate of the medicine's release was determined with the spectrophotometric method, UV-vis. These results were supported by the measurements of the pH and the conductivity of the incubation fluids. Additionally, microscopic observations were performed, together with an X-ray microanalysis (SEM+EDS). Fourier-transform infrared spectroscopic tests were also conducted (FTIR). The gentamicin release from the polymer carrier proceeds the most intensely within the first incubation week, despite the lack of the matrix's degradation at that time. A greater release rate and a bigger amount of the released medicine are stimulated by the presence of phase boundaries. This means that locating gentamicin in the phase modifying the matrix (fibres, capsules) facilitates the medicine's release from the material. The antibiotic's release from calcium alginate capsules proceeds faster than from PLA fibre ones, which is probably connected with their significant swelling in the water environment, facilitating the medicine release.